자궁경부암 세포주들과 Nude mouse 에 이종이식한 자궁경부암 세포에서 아데노바이러스와 리포펙틴을 이용한 종양억제 유전자 ( p53 ) 의 이입율

여경아(Kyeong A Yeo),정재은(Jae Eun Jung),이혜영(Hye Young Lee),이방현(Bang Hyon Lee),김태형(Tae Hyung Kim),천병수(Byeung Soo Cheung),노민석(Min Suk Rho),이준모(Joon Mo Lee),남궁성은(Sung Eun Namkoong),박용석(Yong Seok Park),김종국(Ch 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.6

N/A Background: The classical treatment of the cervical cancer is surgery, radiotherapy, chemotherapy. Even though the improvement of treatment successful rate, conventional therapy has some limitations. Recent cutting edge of cancer therapy has been developed in gene level including understand the biological characteristics of the cancer cells, enhance the human immune response, suppress the cancer cell proliferation. Therefore, the gene therapy is proposed to new treatment strategy. Purpose: The transfection efficiency of cervical cancer cell lines and cervical cancer cell line xerografted nude mouse was investigated by transfection of liposome and infection of adenovirus mediated suppressor(p53) and reportor(LacZ) gene. Method: The cervical cancer cell lines was used in this study were CaSki, SiHa (HPV16 positive, wild type p53 gene), HeLa, He]aS3(HPV18 positive, wild type p53 gene) and C33A, HT3(HPV negative, mutant p53). Direct plasmide and AdCMVp53 gene transfection was performed by using liposome system (pRcCMVLacZ / lipofectin, FuGene 6, Ca-phosphate). LacZ gene was used as the reportor gene for the transfection efficiency evaluation. Expression of p53 in cell lines and tumor tissue was confirmed by western blot and immunohistochemical staining. Xenografted nude mouse of SiHa cell line was infected by AdCMVp53 and AdCMVLacZ. Transfection efficiency was observed by same as above. Results: In cervical cancer cell lines, gene transfection using liposome system(pRcCMVLacZ/lipofectin, FuGene 6, Ca-phosphate)revealed different transfection efficiency, especially pRcCMVLacZ in Fugene 6 showed 18-40% of high transfection efficiency in 6 cervical cancer cell lines by X-gal staining and AdCMVp53 showed 95-98% of the high transfection efficiency in HeLa, C33A. AdCMVp53 was significantly expressed at 2-5days after injection xenografted nude mouse on the western blot and transfection efficiency was 19.79±5.36, 26.26 ± 11.69, 14.77±3.98,15.99 ±6.43%(day1-5). AdCMVLacZ were found to immunohistochemistry analysis, in vivo transFection efficiency was 61.26±4.66, 59.63±9.12, 29.46±14.33, 31.73±22.64%(day 1-5) at ×200 and 88.68.65, 70.85±20.94, 40.75±25.44, 48.21±10.97% (day 1-5) at ×400. Conclusion: As a results , adenovirus-mediated transfection efficiency was higher in vivo experiment compared to cell lines. These high efficiency of adenovirus-mediated suppressor gene(p53) could become a significant meaningful data gene therapy strategy both transgenic mice and cervical cancer cell lines.

터어키양 안장내 지주막 낭종에 의한 저성선자극호르몬성 저성선증

조현희(Hyun Hee Jo),여경아(Kyeong A Yeo),김진홍(Jin Hong Kim),길기철(Ki Cheol Kil),최형주(Hyoung Ju Choi),류순원(Sun Won Yoo) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.7

Primary amenorrhea due to intrasella arachnoid cyst is a very rare disease and require careful and frequent evaluation because may produce intracranial hemorrhage, elevated intracranial pressure and rapid expansion. Surgical intervention is needed only when visual disturbance, hypopituitarism or enlarging lesion is shown. Thus, we present a case of primary amenorrhea due to intrasella arachnoid cyst which was resected through the transsphenoidal approach.

CNG차량의 연료공급라인용 Check Valve Seal의 거동해석

유재찬(Jae-Chan Yoo),여경모(Kyeong-Mo Yeo),박태조(Tae-Jo Park),강병루(Byeong-Roo Kang) 한국트라이볼로지학회 2006 한국트라이볼로지학회지 (Tribol. Lubr.) Vol.22 No.6

In CNG (Compressed natural gas) fuel supply line, whose main components are receptacle and check valve are used to charge high pressure gas to the tank of NGV (Natural gas vehicle). It is reported that the seal is separated occasionally form valve seat and results in blockage of gas flow. In this paper, MARC is used to investigate the reasons of seal separation and suggest design improvements. The static gas pressure distributions acting on the seal which calculated using FLUENT are considered to investigate accurate seal deformation behaviors. Deformed seal shapes are obtained for various amounts of seal interference and its location, gas pressure distributions and Young's modulus of the rubber used. The results showed that the reasons of seal separation problems are verified theoretically, and suggested examples of new design method. Therefore the present numerical methods can be applied in designing and performance analysis of rubber seals adopted in high pressure fluid machineries.

수소자동차의 연료주입라인용 Check Valve 내의 유동해석

박경택(Park, Kyong-Taek),여경모(Yeo, Kyeong-Mo),박태조(Park, Tae-Jo),강병루(Kang, Byeong-Roo) 한국신재생에너지학회 2007 한국신재생에너지학회 학술대회논문집 Vol.2007 No.06

The high pressure hydrogen gas refueling system is required for fuel cell vehicle. In this paper, a commercial computational fluid dynamics (CFD) code, FLUENT is adopted to investigate the gas flow characteristics inside the check valve for various refueling and tank pressures. The results showed that the choking phenomena can occur for certain refueling pressures, therefore refueling processes should be divided by multiple stages. And a design method to prevent the seal departure problem which reported in CNG usages is required.

갱년기 여성에서 호르몬 대체요법시 Vitamin D₃추가투여가 골밀도에 미치는 영향

유순원(Sun Won Yoo),조현희(Hyun Hee Jo),여경아(Kyeong A Yeo),유영옥(Young Ok Lew),권동진(Dong Jin Kwon),정기욱(Gi Wook Chung),김장흡(Jang Heub Kim),김진홍(Jin Hong Kim) 대한산부인과학회 2000 Obstetrics & Gynecology Science Vol.43 No.6

연구목적 : 갱년기 여성에서 호르몬 대체요법 실시시 Vit. D3의 추가 투여가 골밀도에 미치는 영향을 알아보고자 하였다. 연구 대상 : 1992년부터 1998년 사이에 갱년기 클리닉을 방문한 388명을 대상으로 하여 투여 방법에 따라 5군으로 나누었다. A 군 : 에스트로젠 단독요법군 (n=148), B 군 : 에스트로젠-프로제스테론 병합요법군 (n=103), C군 : 에스트로젠 + Vit. D3 투여군 (n=36), D 군 : 에스트로젠, 프로제스테론 + Vit.D3 투여군, E 군 : 비 투여군 (n=60). 연구 방법 : 호르몬 대체 요법 시작 전과 시작 후 2년 간격으로 이중에너지 방사선 흡수 계측법(DEXA, Hologic)을 사용하여 요추의 골밀도와 대퇴골 경부의 골밀도를 측정하였다. 통계 : 연구 대상군들의 골밀도 변화 비교는 paired t-test, one way ANOVA test와 student's t-test를 실시하였다. 결과 : 각 치료군간의 환자특성 및 기저 골밀도 치에 따른 환자특성은 차이가 없었으며, 프로제스테론 병합투여 여부는 골밀도에 의의있는 영향을 끼치지 않았다. Vit.D3 추가 투여시 기저 골밀도가 정상인 군은 대퇴 골두와 요추에서 단독 호르몬 치료군에 비해 모두 의의 있는 증가소견을 보이지 않았으나 기저 골밀도가 골결핍을 보인 군은 에스트로젠과 Vit.D3 투여군의 경우 요추에서 6.1±3.6% (p > 0.05), 대퇴 골두에서 8.5±3.1%(p < 0.05), 프로제스테론 병합요법과 Vit.D3 투여군의 경우 요추에서 5.9±4.1% (p > 0.05), 대퇴골두에서 6.7±4.1%(p < 0.05), 골다공증을 보인 군은 위순서대로 각각 6.7±3.4% (p > 0.05), 11.0±2.1%(p < 0.05), 6.1±1.1% (p > 0.05), 9.1±0.8%(p < 0.05)을 보였다. 결론 : 갱년기 호르몬 치료시 Vit.D3 추가투여는 기저 골밀도가 정상인 군에서는 골밀도 증가에 의의가 없으며, 골결핍증과 골다공증이 있는 여성은 Vit.D3 추가 병합투여가 골밀도 증가 도움을 준다고 할 수 있지만 요추보다는 대퇴골두 골밀도 증가에 더욱 효과가 있다고 할수 있다. Objective : To determine the effect of the addition of Vit. D3 (1,25-Dihydroxychole calciferol D3) to the conventional postmenopausal hormone replacement therapy on bone mineral density(BMD) Design : A 2-year retrospective , randomized study Setting : Department of Obstetrics and Gynecology of Catholic university hospital Patients : 388 postmenopausal women were recruited and divided into 5 groups according to treatment regimen; A: conjugated estrogens only treated group(n=146), B : conjugated estrogens and progesterone treated group(n=103), C : conjugated estrogens and Vit.D3 treated group (n=36), D : conjugated estrogens, progesterone and Vit.D3 treated group (n=41), E : control group (n=60). Methods : The bone mineral density of the lumbar spines and femoral neck were determined by dual-energy X-ray absorptiometry(DEXA) every 2 years.Statistics : The difference between before and after treatment was determined by paired t-test. The comparison among the groups were determined by one way ANOVA test and student's t-test Results : The addition of progesterone to estrogen showed insignificant increase in the lumbar and femoral neck BMD. The addition of Vit. D3 compared with conventional hormone replacement therapy insignificantly influened bone density in women with initially normal BMD, but definitely increase in women with initially osteopenic and osteoporotic BMD of femoral neck rather than lumar spine(p<0.05). Conclusion : The use of Vit. D3 combined with postmenopausal estrogen replacement effects the increase of BMD in low bone density than normal bone density, especially femoral neck.

자색고구마 anthocynin 색소의 대량추출 및 농축

임종환(Jong-Whan Rhim),이장욱(Jang-Wook Lee),조재선(Jae-Sun Jo),여경목(Kyeong-Mok Yeo) 한국식품과학회 2001 한국식품과학회지 Vol.33 No.6

화해를 입은 철근콘크리트 보의 다양한 보수보강 방법에 따른 구조거동 파악을 위한 유한요소해석 연구

이여경(Lee, Yeo Kyeong),류진아(Ryu, Jin A),김희선(Kim, Hee Sun),신영수(Shin, Yeong Soo) 한국콘크리트학회 2015 한국콘크리트학회 학술대회 논문집 Vol.2015 No.5

소규모 건축구조 기준

이여경(Lee, Yeo-Kyeong),신영수(Shin, Yeong-Soo) 대한건축학회 2022 建築 Vol.66 No.3

장신구 디자인과 보석세팅의 효과적 연계에 대한 연구

박여경 ( Park Yeo-kyeong ),안일훈 ( Ahn Il-hoon ) 한국디자인트렌드학회 2017 한국디자인포럼 Vol.56 No.-

연구배경 활짝 핀 꽃이 아름답듯이 확연한 의도와 뛰어난 감각에 의해 디자인된 주얼리는 아름답다. 세상에는 수많은 보석이 있고, 아름다운 보석들을 다양한 디자인과 적절한 보석 세팅기법을 활용하여 훌륭한 장신구로 탄생시키는 것이 모든 주얼리 디자이너들의 소망이다. 아름답고 고귀한 다이아몬드도 장신구에 적절히 세팅되지 않는다면 진정한 가치를 발휘할 수 없기에 디자인에 걸맞은 적절한 보석 세팅 기법을 선택하는 것이 좋은 디자인을 하기 위한 기본이다. 연구방법 장신구 디자인에서 보석의 세팅에 초점을 맞추어 보석 세팅이 디자인적 요소로 주요한 단서를 제공하고 있고, 또한 수많은 장신구가 보석과 함께 세팅되고 있으므로 보석 세팅의 영향력이 크다는 것을 전제로 디자인적 요소를 고려한 상황과 여건에 맞는 아름다운 장신구를 디자인하는 데 의의가 있다. 보석세팅을 이용한 디자인 개발에 앞서 현재 장신구에 사용되고 있는 다양한 보석세팅 기법과 이를 활용한 장신구 디자인을 자료 조사하였다. 보석 세팅 기법의 종류와 설명은 관련 논문 및 서적, 그리고 인터넷정보, 업계 관행 등을 참고로 인용하였으며, 업계에서 오랫동안 종사한 세공기술자와 업계대표와의 인터뷰를 참고하였다. 인터넷과 서적에서 장신구의 보석 세팅별 사진 이미지와 보석 세팅 해석 사진을 첨부하는 동시에 캐드를 프로그램을 활용하여 캐드 이미지 그림을 첨부하였다. 연구결과 이와 같이 조사된 자료 내용을 통하여 연구한 결과는 각각의 보석 세팅 기법에 따라 시각적으로 다른 이미지를 가지게 되므로 시각적 자극을 받게 되는 사람에게 다양한 장신구 이미지 정보를 제공할 수 있으며, 보석 세팅 방법에 따른 적절한 디자인 개발을 할 수 있다. 결론 보석세팅의 다양한 활용성이 디자인적 요소로 충분히 응용될 것이며, 장신구 디자인에 있어 긍정적인 결과가 있을 것으로 기대한다. Background As broad-blown flower is beautiful, jewelry designed by decided intention and outstanding sense is beautiful. There are many jewels in the world, and a wish of all jewelry designers is to produce these beautiful jewels as worthy jewelry with various designs and proper jewelry setting technique. If diamond emitting beautiful light is not properly set in jewelry, it is difficult for this diamond to create true value, so to consider the proper jewelry setting technique suited to design is to find the good design. Methods This study focuses on the setting of jewels in the jewelry design, so jewelry setting offers a major clue as a design factor, and many jewelries have been set with jewels, so it is meaningful to design a beautiful jewelry suited to conditions considering a design factor on the premise that jewelry setting is influential. Before the design development using the jewelry setting, I looked at the various jewelry setting techniques used in the jewelery and jewelry design using it. Related thesis and book, internet information, and practice in the business world were consulted and quoted about type and explanation of jewelry setting technique, and interview with CEO and jewelry worker working in the jewelry industry for ages was consulted. The researcher added jewelry setting analysis picture and image by jewelry settings of jewelry in internet and book, created jewelry supposed to be set by lino CAD principle of jewelry setting. Result Each jewelry setting technique has a visually different image based on above data for research, so a variety of jewelry image information can be offered to people with visual stimuli, and the study on the development of proper design according to jewelry setting methods is meaningful. Conclusion Diversity and use of jewelry setting will be fully applied as a design factor through this study, and there will be a positive result of jewelry design.

분리형 띠철근을 적용한 철근콘크리트 기둥의 축력거동 평가

이여경(Lee, Yeo Kyeong),최연희(Choi, Youn Hee),신영수(Shin, Yeong Soo) 한국콘크리트학회 2018 한국콘크리트학회 학술대회 논문집 Vol.30 No.2