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자궁경부암 세포주들과 Nude mouse 에 이종이식한 자궁경부암 세포에서 아데노바이러스와 리포펙틴을 이용한 종양억제 유전자 ( p53 ) 의 이입율
여경아(Kyeong A Yeo),정재은(Jae Eun Jung),이혜영(Hye Young Lee),이방현(Bang Hyon Lee),김태형(Tae Hyung Kim),천병수(Byeung Soo Cheung),노민석(Min Suk Rho),이준모(Joon Mo Lee),남궁성은(Sung Eun Namkoong),박용석(Yong Seok Park),김종국(Ch 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.6
N/A Background: The classical treatment of the cervical cancer is surgery, radiotherapy, chemotherapy. Even though the improvement of treatment successful rate, conventional therapy has some limitations. Recent cutting edge of cancer therapy has been developed in gene level including understand the biological characteristics of the cancer cells, enhance the human immune response, suppress the cancer cell proliferation. Therefore, the gene therapy is proposed to new treatment strategy. Purpose: The transfection efficiency of cervical cancer cell lines and cervical cancer cell line xerografted nude mouse was investigated by transfection of liposome and infection of adenovirus mediated suppressor(p53) and reportor(LacZ) gene. Method: The cervical cancer cell lines was used in this study were CaSki, SiHa (HPV16 positive, wild type p53 gene), HeLa, He]aS3(HPV18 positive, wild type p53 gene) and C33A, HT3(HPV negative, mutant p53). Direct plasmide and AdCMVp53 gene transfection was performed by using liposome system (pRcCMVLacZ / lipofectin, FuGene 6, Ca-phosphate). LacZ gene was used as the reportor gene for the transfection efficiency evaluation. Expression of p53 in cell lines and tumor tissue was confirmed by western blot and immunohistochemical staining. Xenografted nude mouse of SiHa cell line was infected by AdCMVp53 and AdCMVLacZ. Transfection efficiency was observed by same as above. Results: In cervical cancer cell lines, gene transfection using liposome system(pRcCMVLacZ/lipofectin, FuGene 6, Ca-phosphate)revealed different transfection efficiency, especially pRcCMVLacZ in Fugene 6 showed 18-40% of high transfection efficiency in 6 cervical cancer cell lines by X-gal staining and AdCMVp53 showed 95-98% of the high transfection efficiency in HeLa, C33A. AdCMVp53 was significantly expressed at 2-5days after injection xenografted nude mouse on the western blot and transfection efficiency was 19.79±5.36, 26.26 ± 11.69, 14.77±3.98,15.99 ±6.43%(day1-5). AdCMVLacZ were found to immunohistochemistry analysis, in vivo transFection efficiency was 61.26±4.66, 59.63±9.12, 29.46±14.33, 31.73±22.64%(day 1-5) at ×200 and 88.68.65, 70.85±20.94, 40.75±25.44, 48.21±10.97% (day 1-5) at ×400. Conclusion: As a results , adenovirus-mediated transfection efficiency was higher in vivo experiment compared to cell lines. These high efficiency of adenovirus-mediated suppressor gene(p53) could become a significant meaningful data gene therapy strategy both transgenic mice and cervical cancer cell lines.
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최훈,이홍균,김복린,김명주,유태환,김용범,김종국,서정식,양창현 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.11
The carcinoma of the uterine cervix is the most prevalent life-threatening cancer among female genital tract cancers. However, it can be cured by early diagnosis and treatment. So, the purpose of this study was to improve the prognosis of this disease by early detection, diagnosis, and treatment through analyzing the clinical profiles. From September 1989 to December 1994, 159 cases of the carcinoma of the uterine cervix diagnosed and treated at department of obstetrics and gynecology, Sanggye hospital were evaluated through medical records. The results were as follows: 1) the common chief complaint were abnormal Pap smear without symptome(40.3%), vaginal spotting(40.9%), and contact bleeding(11.9%). 2) The distribution of clinical stage by FIGO classification were stage I a1(32.6%), stage I a2 (6.3%), stage I b(28.9%), stage II a(16.3%), stage II b(11.4%), stage III(3.8%), and stage IV(1.2%). 3) The most common histologic type was squamous cell carcinoma(94.3%). 4) The types of treatment were operation alone(65.5%), operation and radiotherapy(12.6%), radiotherapy(13.9%), neoadjuvant chemotherapy and operation(3.2%), radiotherapy and chemotherapy(3.7%), and conservative treatment(1.2%). 5) Median follow up time was 48.3 months and median disease free survival interval were I a(48.3 months), I b(44.4 months), II a(41.5 months), II b(33.3 months), III(21.2 months), and IV(14.1 months). With these results, we concluded that the cervical intraepithelial neoplasam and early carcinoma of uterine cervix are expected to be cured completely and even the advanced carcinoma of uterine cervix can be treated with better prognosis by early detection, diagnosis, and treatment through periodical Pap smear and systematic examination.
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최훈,이홍균,김복린,유태환,김용범,김종국,서정식,양창현,민준식 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.10
Peripartal hysterectomy is associated with high risks of severe blood loss, postoperative complications, and maternal morbidity. We performed this study to identify the risk factors of peripartal hysterectomy and to reduce the postoperative complications and maternal morbidity. In this study, the outcomes of 28 cases of peripartal hysterectomy performed at the department of obstetrics and gynecology, Sanggye Paik Hospital from Aug. 1989 to Dec. 1995 were reviewed and associated risk factors were identified. The results were as follows; There were 24,138 deliveries during this period, and peripartal hysterectomy was performed in 12 cases among 5,502 cesarean deliveries(0.21%) and in 16 cases among 18,636 vaginal deliveries(0.08%). The incidence of peripartal hysterectomy was 1.16%(28/24, 138). The age of patients ranged from 26 to 40 years old. The maternal mortality and morbidity were 7.1%(2/28) and 64.2%(18/28), respectively. The indications for peripartal hysterectomy were uterine atony(46.4%), placenta accreta and/or percreta(38.7%), and uterine rupture(21.4%) in orders. The associated risk factors of hysterectomy were prior cesarean delivery(32.1%) and placenta previa(21.4%). The postoperative complications were febrile morbidity(72.2%), bladder injury(16.7%), and disseminated intravascular coagulopathy(11.2%). From the above results, we concluded that cesarean delivery, prior cesarean delivery, placenta previa, placenta accreta, and uterine atony were risk factors for peripartal hysterectomy.
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