경계성 난소종양의 임상적 고찰

남궁성은(Sung Eun Namkoong),이준모(Joon Mo Lee),이상희(Sang Hee Lee),배태욱(Tae Wook Bae),이노준(No Jun Lee),김찬주(Chan Joo Kim),박태철(Tae Chul Park) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.3

N/A Objectives: The purpose of this study is to investigate the clinicopathologic characteristics and the risk factors affecting the recurrence in patients with borderline ovarian malignancy. Methods: From January 1996 to January 2001,37 patients with borderline tumors of the ovaries were retrospectively investigated in the Department of Obstetrics and Gynecology, Catholic University, Kangnam and Uijongbu St. Mary's Hospital. Several clinicopathologic factors including DNA ploicly was analyzed for the prognosis and recurrence. sAnalysis for the kinds of treatment and recurrence were conducted to test the prognostic significance of several clinicopathologic factors including DNA analysis. Results: Histologically, 27 borderline tumors were serous,9 were mucinous and 1 was mixed epithelial type. The FIGO stage I was 91.8%(34/37)and stage II was 8.2%(3/37).Mean value of CA125 in mucinous borderline malignancy was significantly higher (162.4 IU/mL)than serous types (52.2 IU/mL)(p<0.05). The patients with elevated CA 125 levels (>35 IU/mL)were 56.3%(9/16)in serous type and 75%(6/8)in mucinous tumors. Ten of 13 cases with DNA flow cytometry showed aneuploidy (76.9%).When considering pathologic types between diploid and aneuploid groups, there were no statistically significant differences. However, the patients with old age (>40) were more likely to be aneuploid (p<0.05).Mean duration of follow-up investigation was 26 months after primary operation. In this period, only one patient with serous borderline tumor stage Ia had recurrence on the contra-lateral ovary at 13-month. Conclusion: Data from this study showed that the majority of borderline tumors have good prognosis. And young patients who have not completed childbearing can be safely treated with unilateral salpingo-oophorectomy and omentectomy in stage I diploid tumor. In ovarian bordeline tumors, further studies on DNA ploidy would be needed.

특별강연 : 한국 여성에서 자궁경부암 치료의 전망

남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 2006 대한산부인과학회 학술대회 Vol.92 No.-

자궁경부 비정상 세포진검사(ASCUS/LG SIL)의 처치방법

남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 1996 서울심포지움 Vol.3 No.-

자궁경부암을 유발하는 HPV E6 단백질에 의한 p73 단백질의 불활성화 : p53과 무관한 E6의 새로운 기능

남궁성은(Sung Eun Namkoong),김승조(Seung Jo Kim),김은주(Eun Joo Kim),엄수종(Soo Jong Um),박종섭(Jong Sup Park) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.11

N/A Objective: Human papillomavirus (HPV) is strongly implicated as a causative agent in the etiology of cervical cancer. Of its gene products, E6 and E7 oncoproteins play major roles by inactivation of cellular p53 and pRb tumor suppressor proteins, respectively. However, it has been recently suggested that p53 and/or pRb-independent functions of E6 and E7 are involved in cervical carcinogenesis. The purpose of this study is to identify novel a cellular target, p73, of E6 and to determine how E6 inactivates p73 function, Methods: The interaction between E6 and p73 were identified by the yeast two-hybrid assay in vivo and the GST pull-down assay in vitro. The function of the interaction was determined by transient transfections using p21 promoter-CAT reporter plasmid. The molecular mechanism underlying the functional significance of the interaction was further assessed by in vivo and in vitro protein degradation assays, and gel mobility shift assays. Results: Yeast two-hybrid and GST pull-down assays indicate a physical interaction between p73 and either HPV-16 or HPV-11 E6 proteins in vivo and in vitro, respectively. Transactivation domain (amino acid residues 1-49) is found to be absolutely required for this interaction. Transient co-expression of E6 significantly inhibits the p73-mediated activation of p21 promoter in a p53-defective C33A cell line. Using Ga14-p73 fusion protein, we demonstrate that E6 inhibition of p73 transactivation function is independent of sequence-specific DNA binding, which is confirmed by direct electrophoretic mobility shift assay. Moreover, E6 inhibits p73 function by interfering with the activity of the amino-terminal activation domain. The protein degradation assays in vivo and in vitro indicate that p73, unlike p53, is not susceptible to E6-dependent proteolysis. Conclusion: Throughout this study, we identified p73 as a novel cellular target of HPV-E6 protein and found that E6 binds p73 through the amino-terminal transactivation domain, and inhibits its transactivation function independent of the protein degradation and DNA binding. These overall results, consequently, suggest that in addition to the inactivation of p53, the functional interference of p73 by HPV-E6 may, at least in part, contribute to E6-mediated cellular transformation.

SiHa 세포주에서 비소화합물 ( As2O3 , As4O6 ) 투여 후 cDNA microarray 를 이용한 유전자 발현의 변화

남궁성은(Sung Eun Namkoong),서미영(Mi Young Seo),박은경(Eun Kyung Park),팽기영(Gi Young Pang),김종국(Chong Kook Kim),박용석(Yong Serk Park),안웅식(Woong Shick Ahn),이준모(Jun Mo Lee),김도강(Do Gang Kim) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.7

목적 : SiHa 세포주에서 비소 화합물에 의한 세포성장 억제 효과와 유전자 발현 변화를 cDNA microarray 기술을 이용하여 확인하고자 한다. 연구 방법 : 자궁경부암 세포주인 SiHa의 성장억제에 관한 연구를 위하여 96 well plate에 103의 SiHa cell을 분주한 후, 비소화합물을 처리하여 세포 성장억제를 MTT assay 방법을 이용하여 확인하였다. 한편 384개의 유전자로 제작된 DNA chip을 이용하여 자궁경부암 SiHa 세포주에 비소화합물 1 uM을 처리하고 48시간 후의 total RNA를 추출하여 gene expression profile을 관찰하였다. 결과 : 자궁경부암 세포주인 SiHa cell의 성장억제에 관한 연구에서 비소화합물을 각각 0.5, 1, 2, 3, 4, 5 uM로 처리하여 4일간 관찰한 결과 4일째에 As2O3의 경우 각각 9.2, 56, 89, 93, 96, 96%, As4O6의 경우 54, 84, 84, 85, 87%의 세포증식억제가 관찰되었다. DNA 칩의 유전자 발현에 있어서 As2O3의 경우, 2배 이상 변화한 유전자는 thymidylate synthetase, cyclin B1, CDC 20 등 약 47여개 정도였으며, As4 O6의 경우, 대조군과 비교하여 2배 이상 변화한 유전자는 CDC 20, cyclin B1, primase, proliferating cell nuclear antigen 등 약 78여 개의 유전자가 변화한 것으로 나타났다. 결론 : SiHa cell에서 비소화합물에 의한 세포성장억제를 확인할 수 있었으며 유전자 발현의 변화에 있어서는 As2O3보다는 (47 genes) As4O6가 더 많은 변동을 일으켰으며 (78 genes) 이중 공통적으로 증가한 유전자는 6개이고 공통적으로 감소한 유전자는 26개로서 이에 대한 기능적인 면은 더 많은 연구가 필요하리라 생각된다. Objective : To obtain information on the growth inhibition effect of arsenic compounds and gene expression profiles using cDNA microarray technique in SiHa cell lines. Methods : We cultured 103 SiHa cell in 96 well plate and we investigated growth inhibition effects using MTT assay and also we performed gene expression profile experiment using 384 cDNA chip in SiHa cell after exposure of arsenics (As2O3, As4 O6 - 1 μM) for 48 hrs. Results : Arsenics (As2O3, As4 O6) inhibit the growth of SiHa cells (As2O3: 0.5, 1, 2, 3, 4, 5 μM - 9.2, 56, 89, 93, 96, 96%, As4O6: 0.5, 1, 2, 3, 4, 5 μM- 54, 84, 84, 85, 85, 87%) in 4 days culture. As2O3 and As4O6 induced apoptosis in SiHa cells. After exposure of As2O3, 47 genes were changed more than 2 times (eg, thymidylate synthetase, cyclin B1, CDC 20). In case of As4O6, 78 genes were changed more than 2 times (eg, CDC 20, cyclin B1, primase, proliferating cell nuclear antigen). Conclusion : we observed arsenic compound (As2O3, As4 O6) inhibit the growth of SiHa cell. In gene expression profiling experiment, 78 genes was changed the expression level 2 times more than that of reference RNA after treatment of As4O6 and 47 genes after treatment of As2O3. Through these result, we thought more study need in functional genomics after arsenic treated cervical cancer cells.

ASCUS나 LSIL이 보고된 폐경여성에서 고등급 자궁경부병변을 예측하는데 있어 HPV viral Load 값의 의의

이해남 ( Hae Nam Lee ),정인철 ( In Cheul Jeung ),이용석 ( Yong Sek Lee ),박은경 ( Eun Kyung Park ),김찬주 ( Chan Joo Kim ),박종섭 ( Jong Sup Park ),남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 2008 Obstetrics & Gynecology Science Vol.51 No.11

목적: Unsatisfactory colposcopy 소견이면서 ASCUS나 LSIL이 보고된 폐경여성에서 고등급 자궁경부병변을 예측하는데 HPV viral load 값이 도움이 되는지 평가하였다. 방법: HPV 검사가 양성이면서 ASCUS나 LSIL이 보고된 폐경여성들 중 진단적 conization을 시행한 54명을 분석하였다. HPV검사는 hybrid capture II을 이용하였다. 자궁경부의 조직학적 결과에 따라 저위험군 (만성자궁경부염, CIN I)과 고위험군 (≥CIN II)으로 나누었다. Viral load값에 따라 100 RLU/PC 미만인 군과 100 RLU/PC 이상인 군으로 나누었고 각 군의 자궁경부 병변들을 서로 비교하였다. 결과: 만성 자궁경부염이 30명, CIN I이 9명, CIN II가 3명, CIN III가 12명이었다. Viral load가 100 RLU/PC 미만인 경우에는 CIN II 이상이 4명 있었으나 100 RLU/PC 이상인 경우에는 11명으로 유의하게 많았으며 (P=0.013) viral load가 100 RLU/PC 이상일 경우 100 RLU/PC 미만일 경우에 비해 ≥CIN II을 가질 상대위험도는 2.043 (95% CI, 1.216~3.432)으로 유의하게 높았다. 결론: Unsatisfactory colposcopy 소견이면서 ASCUS나 LSIL이 보고된 폐경여성에서 HPV viral load값이 높을 경우 ≥CIN II을 가질 위험이 높다. Objective: To evaluate whether determination of the Human Papilloma Virus (HPV) viral load is helpful to predict the presence of high-grade cervical intraepithelial neoplasia (CIN) that requires aggressive treatment in menopausal women who have presented with an unsatisfactory colposcopic finding and ASCUS or LSIL. Methods: In menopausal women with ASCUS or LSIL and with who were positive for HPV, 54 women had diagnostic conization were analyzed retrospectively. We used the Hybrid Capture II method. We classified women in a low-risk group (chronic cervicitis, CIN I) and a high-risk group (≥CIN II) according to the pathological results of conization. We also classified patients into the HPV viral load <100 RLU/PC group and ≥100 RLU/PC group. We analyzed the relationship between the pathological results of conization and viral load. Results: For the conization, 30 women had chronic cervicitis, nine women had CIN I, three women had CIN II and 12 women had CIN III. In the low Hybrid Capture titer-group (<100 RLU/PC), four women had high risk ≥CIN II pathology but in the high Hybrid Capture titer-group (≥100 RLU/PC), 11 women had high risk pathology this finding was statistically significant (P=0.013). Compared to the low Hybrid Capture titer group, the relative risk of high grade CIN (≥CIN II) in the high Hybrid Capture titer group was 2.043 (95% CI, 1.216~3.432). Conclusions: For menopausal women with an unsatisfactory colposcopic finding and also have ASCUS or LSIL women with high HPV viral load might have tendency of ≥CIN II pathology.

자궁경부선암의 임상병리학적 특징과 예후인자

이해남 ( Hae Nam Lee ),김찬주 ( Chan Joo Kim ),최정수 ( Jung Soo Choi ),박종섭 ( Jong Sup Park ),남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.4

한국 여성의 자궁경부에서 아데노 연관 바이러스와 고위험군 인유두종 바이러스 16과 18의 상관관계

이성하 ( Sung Ha Lee ),김병훈 ( Byung Hoon Kim ),곽선영 ( Sun Young Kwak ),배수미 ( Su Mi Bae ),김동재 ( Dong Jae Kim ),김용욱 ( Young Wook Kim ),이준모 ( Joon Mo Lee ),남궁성은 ( Sung Eun Namkoong ),남계현 ( Kye Hyun Nam ),조영래 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.2

목적: 아데노 연관 바이러스의 감염이 인유두종 바이러스에 역상관 관계이며, 자궁경부암 세포 성장을 억제한다고 보고되어 있다. 본 연구는 자궁경부 세포에서 아데노 연관 바이러스와 고위험군 인유두종 바이러스 아형 16과 18의 검출과 아데노 연관 바이러스와 인유두종 바이러스의 연관성을 조사하고자 하였다. 연구 방법: 정상 자궁경부 (49명), 인유두종 바이러스에 감염된 자궁경부 (45명), 상피내 경증 이형증 (31명), 중등도 이형증 (20명), 중증 이 Objective: Previous studies were showed that adenoassocited virus (AAV) infection was had negative effects on human papillomavirus (HPV) infection and that the cervical cancer cell growth is inhibited by AAV infection. We detected of AAV 2 and high-risk H

결핵성 복막염의 임상적 고찰

이성종 ( Sung Jong Lee ),남궁성은 ( Sung Eun Namkoong ),박은경 ( Eun Kyung Park ),임채춘 ( Chae Chun Rhim ),구남식 ( Nam Sik Ku ),류상우 ( Sang Woo Lyu ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.11

목적: 결핵성 복막염의 임상적 및 병리학적 특징과 난소암과의 감별점을 알아보고자 하였다. 연구 방법: 1994년부터 2002년까지 결핵성 복막염으로 진단받은 환자 15명을 대상으로 의무기록을 토대로 임상양상, 진단방법, 치료 등에 대해 알아보았다. 결과: 15명의 환자의 평균연령은 44.4세였고, 16세에서 25세, 56세에서 65세까지가 가장 많은 발생빈도를 보였다. 이들은 복부팽만, 복통, 체중감소, 발열 등의 증상을 보였으며, 일반 혈액검사, 일반 Objective: The aim of this study is to evaluate the clinicopathologic characteristics of tuberculous peritontitis from calcinomatosis peritonei. Methods: From July 1994 to May 2002, fifteen women who were diagnosed tuberculous peritonitis in Kangnam St. M

자궁적출술시 발견된 충수돌기 점액낭종

박은경 ( Eun Kyung Park ),서미영 ( Mi Young Seo ),팽기영 ( Ki Young Paeng ),임채춘 ( Chae Chun Rhim ),김도강 ( Do Kang Kim ),남궁성은 ( Sung Eun Namkoong ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.9

Mucocele arised at the vermiform appendix is uncommon, either benign or malignancy and their clinical presentation is not specific. The preoperative diagnosis is rare,^1 their diagnosis is an incidental event. Mucocele seems to be developed due to chronic obstruction of the lumen of the appendix. Higa and Cowerkers^2 classified 73 cases of mucocele into three clinicopathologic entities; focal or diffuse mucosal hyperplasia, mucinous cystadenoma, mucinous cystadenocarcinoma. Appendiceal mucoceles are rare lesions of the appendix, characterized by a gross enlargement of the appendix from accumulation of mucoid substance within the lumen. It is encountered in only 01-04% of all appendectomies with a female predominance and an average age at the time of diagnosis over 50 years. A case of appendiceal mucocle found during total hysterectomy is presented with a brief review of the leteratures.