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엄대원,박제성,이흔 한국화학공학회 2007 화학공학의이론과응용 Vol.10 No.2
In this research, Raman and 13C NMR spectroscopy are used to investigate the structure and distribution of guest molecules of substituted CH4 hydrates with N2+CO2 mixed gas and CH4+N2+CO2 mixed hydrates. All the structure of the hydrates in this work are revealed to be structure I hydrate from the NMR results. CH4, N2 and CO2 guest molecules are all identified in the substituted hydrate sample according to the Raman results. Therefore CH4 in the small and large cages of hydrates are replaced with N2 and CO2 gas respectively considering the size of guest molecules. From the 13C NMR results, the peaks which indicate CH4 in the small and large cavities independently have not a so great deviation. It means that similar amount of N2 and CO2 gas occupy the small and large cages of CH4 hydrates. Consequently, the flue gas consisting of mainly N2 and CO2 can be applied to recover CH4 gas from CH4 hydrate under the deep sea.
Preventive effect of fermented black ginseng against cisplatin-induced nephrotoxicity in rats
정기원,안준민,엄대원,강기성,김수남 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2
Background: Fermented black ginseng (FBG) is processed ginseng by the repeated heat treatment and fermentation of raw ginseng. The protective effect and mechanism of FBG on cisplatin-induced nephrotoxicity was investigated to evaluate its therapeutic potential. Methods: The free radical scavenging activity of FBG was measured using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In addition, the protective effect against cisplatin-induced renal damage was tested in rats. FBG was orally administered every day at a dose of 150 mg/kg body weight for 10 d, and a single dose of cisplatin was administered intraperitoneally (7.5 mg/kg body weight) with 0.9% saline on the 4th d. Results: The DPPH radical-scavenging activity of FBG (IC50¼ 384 mg/mL) was stronger than that of raw ginseng. The improved DPPH radical-scavenging activity was mediated by the generation phenolic compounds. The decreased cell viability by cisplatin was recovered significantly after treatment with FBG in a dose-dependent manner. Then, the protective effect of FBG on cisplatin-induced oxidative renal damage was investigated in rats. The decreased creatinine clearance levels, which are a reliable marker for renal dysfunction in cisplatin-treated rats, were reduced to the normal level after the administration of FBG. Moreover, FBG showed protective effects against cisplatin-induced oxidative renal damage in rats through the inhibition of NF-kB/p65, COX-2, and caspase-3 activation. Conclusion: These results collectively show that the therapeutic evidence for FBG ameliorates the nephrotoxicity via regulating oxidative stress, inflammation, and apoptosis.
식물성 배지에서 Lactobacillus plantarum의 배양을 위한 배지 최적화
정은지(Eun Ji Jeong),문대원(Dae Won Moon),오준석(Joon Suk Oh),문진석(Jin Seok Moon),엄현주(Hyun Ju Eom),최혜선(Hye Sun Choi),김창섭(Chang Sup Kim),한남수(Nam Soo Han) 한국생물공학회 2012 KSBB Journal Vol.27 No.6
This study was conducted to optim ize the composition of CEM (cabbage extract medium) and cryoprotectants on the growth of Lactobacillus plantarum, a probiotics growing in plant and milk. For this, we analyzed the growth characteristics of Lb. plantarum in CEM and subsequently optimized the medium composition by addition of carbon, nitrogen sources and buffering agents. Among carbon sources, glucose showed the best result to increase the cell density after dilution of CEM. When 0.5% yeast extract and 1% soy peptone were supplemented in the diluted CEM, Lb. plantarum grew up to the maximum cell density. Addition of buffering agents in CEM was not significantly effective to increase the cell density. Meanwhile, addition of 12% skim milk, 5% sucrose and 0.5% glycerol showed a cryoprotective effect against cell damage of Lb. plantarum during freeze drying process showing high survival rate after 150 days. This optimized CEM can be used for economical production of bacterial cells particularly originated from a plant-related ecosystem.
곽진호,장혁재,최건무,박천수,엄대원,김성수,한덕종,김인구 대한이식학회 2015 Korean Journal of Transplantation Vol.29 No.1
A 47-year-old man developed chronic alcoholic liver cirrhosis and end-stage renal disease. He underwent blood-type-compatible liver transplantation with a graft from his daughter. After 8 months, sequential ABO-incompatible (ABOi) kidney transplantation was performed, with his brother as the donor (A to O). The patient had anti-A antibody titers (1:256). We performed pretransplant desensitization, including administration of rituximab, mycophenolate mofetil, tacrolimus, and prednisolone 2 weeks before the scheduled transplantation, and plasmaphresis (PP) and administered an intravenous immunoglobulin injection. The patient underwent PP before kidney transplantation until the anti-A antibody titer was <1:8. The patient achieved normal renal function within 4 posttransplantation days. Postoperative bleeding (diffuse hemorrhage) requiring additional blood transfusions and radiological intervention (drainage procedure) occurred 9 days after transplantation. The patient was discharged on day 20 of hospitalization. Nine months after the kidney transplantation, the recipient’s and donor’s liver and kidney functions were normal. ABOi renal transplantation after liver transplantation can be successfully performed in patients with high baseline anti-ABO antibody titers after preconditioning with rituximab and PP, and quadruple immunosuppressive therapy. However, caution is required regarding an increased risk of bleeding complications.