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양주현,김성태 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.5
Chicken primordial germ cells (cPGCs) are founder germ cells in embryonic stage of development that eventually give rise to sperms or oocytes. Currently cPGCs are only known cells enabling germline transmission in chicken and their cultivation protocols were recently established. Although genome modifications of chickens are now theoretically possible using cPGCs, there are still several hurdles to overcome to practically use cPGCs as mediators for chicken transgenesis. First, efficiency of gene delivery into cPGCs remains low with current methods. Second, there aregene silencing mechanisms against the expression of foreign genes in cPGCs. In this study, we successfully increased the efficiency of gene delivery in cPGCs by taking advantage of the TTAAspecific piggybac transposon system. Moreover, a pipette-type electroporator significantly enhanced transfection efficiency up to 5-fold compared withcuvette-type methods. Taken together, the technological advances in our study will provide practical benefits for the application to fulfill genetic modifications of chicken genome.
방문 돌봄 노동자의 근골격계 자각증상, 수면문제와 주관적 정신건강 간의 관련성: 수면문제의 매개효과를 중심으로
양주현,이은정,박보현,정인옥 한국직업건강간호학회 2022 한국직업건강간호학회지 Vol.31 No.1
Purpose: This study aimed to identify the relationship among musculoskeletal problems, sleep problems, and self-rated mental health of home-care workers. Methods: Data were collected from 447 home-care workers spanning three occupation types: life supporters for the elderly, home-visit caregivers, and life supporters for the disabled. Musculoskeletal problems, sleep problems, and self-rated mental health were assessed using structured questionnaires. Factors affecting self-rated mental health were analyzed using multiple regression. SPSS was used to test the mediating effects of sleep problems on musculoskeletal problems and self-rated mental health. Results: Among the general characteristics, the variables that showed significant differences in musculoskeletal problems were monthly income level, caring-related career duration, weekly working hours, and occupation type; and the variable that showed significant differences in self-rated mental health was occupation type. Among the occupation types, supporters for the disabled had the most musculoskeletal problems and the lowest self-rated mental health. Musculoskeletal problems among home-care workers had a direct negative effect on self-rated mental health and indirect negative effects on sleep problems. Conclusion: Measures are needed to reduce the differences in working conditions and health status among the occupation types of home-care workers. Considering the relevance between the health issues of home-care workers, the development of a carefully designed health promotion strategy is required.
노인장기요양 재가서비스 종사자들이 서비스 제공과정에서 경험하는 어려움: 방문간호사, 사회복지사, 요양보호사를 중심으로
양주현,박보현,진보영,김미경 한국노년학회 2020 한국노년학 Vol.40 No.6
The objective of this study was to investigate the difficulties experienced by long-term care service providers working in home settings—specifically, home-visit nurses, social workers, and paid caregivers. Study participants consisted of 18 providers, including 7 home-visit nurses, 4 social workers, and 7 paid caregivers. The in-depth interviews were conducted in December 2018, with each lasting about 2 hours. The data were analyzed by qualitative content analysis. Common issues among participants included difficulty in achieving rapport with clients and their families, lack of communication among providers, and an inefficient compensation system for long-term care insurance. Home-visit nurses identified issues with lack of standardized nursing intervention, and difficulty in providing comprehensive nursing care due to treatment-focused interventions. Social workers pointed out ‘the difficulty of mediation due to inappropriate demands of the clients(family) as well as family members’, ‘lack of knowledge about clients’ condition change’. Lastly, paid caregivers discussed difficulties such as ‘lack of understanding about the role of caregivers’, ‘overtime due to lack of understanding of the working hours’. To improve the quality of home-based long-term care services and expand the number of clients served, policies and interventions should be developed to reduce the difficulties experienced by service providers.
Ti oxide 기판에 오염된 유사 G-series 신경 화학작용제의 광분해 연구
양주현,박소정,홍성민,김정권,이기주,정희수,손영구 한국진공학회 2021 한국진공학회 학술발표회초록집 Vol.2021 No.2
본 연구는 UVC, UVB, UVA 광원을 이용하여 Ti oxide 기판에 오염된 유사 G-series 신경 화학작용제인 dimethyl methylphosphonate, dimethyl phosphite, diethyl methylphosphonate, dimethyl phosphite의 광분해 연구에 대해 수행하였다. 휘발성의 2차 광분해 부산물은 gas chromatography로 분석하고, 기판 위 잔여물은 scanning electron microscopy와 energy-dispersive X-ray spectrometry로 분석하였다. 본 연구는 광원에 따른 광분해 부산물 분석을 통해 유사 G-series 신경 화학작용제의 광분해 메커니즘을 추적하고, 이차 생성물의 2차 오염원 처리 및 다양한 신경 화학작용제의 레이저 광분해 연구에 유용한 정보를 제공할 것으로 본다.
미숙아의 백질연화증 (Periventricular Leukomalacia) : 자기공명영상과 초음파진단의 비교연구
양주현 대한영상의학회 1993 대한영상의학회지 Vol.29 No.6
MRI(magnetic resonance imaging) had been the most up-to-dated modality in evaluating white matter disease in recent years, shereas US (ultrasonogram) has been used extensively in diagnosis of neonatal PVL (peri-ventricular leukomalacia) conventionally. We evaluated the diagnostic value of MRI by reviewing the MR findings and correlation of MR and US of PVL in 11 preterm infants. Evaluation criteria were MR signal intensity and discrimination of PVL on each pulse sequences and comparision between MR and US findings performed simultaneously, on the extent of PVL, size of the largest cyst and detectability of hemorrhagic lesion, MR findings of 1 cases of PVL were of low signal patterns on T1W1 (T1 weighted image) in 7, low signal patterns on PDWI (proton density weighted image) in 9, iso signal patterns on T2W1(T2 weighted image) in 8 and low signal patterns on STIR (short time inversion recover) in 7 cases. The lesions of 11 PVL were well discriminated in all 11 cases of T1W1, 7 cases of STIR, 5 case of PDWI and 2 cases of T2W1. The lateral ventricle was diffuse dilated (n=1) and focally dilated (n=3) in atrial area. In the comparative study, MR presented more extensive lesions in 7 cases, larger cysts in 6 cases out of 7 PVL and more definitive hemorrhage in 3 cases out of 4 cases than sonography, In conclusion, MR was more valuable than sonography in evaluating the extent of lesions, size of the largest cystic lesion and detetion of hemorrhage. T1WI and STIR images were more useful in detection of PVL than T2WI and PDWI.