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탄소나노튜브를 이용한 Microfluidic Chip용 나노필터 제작
안윤호(Yun-Ho An),송시몬(Simon Song) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6
This paper describes the fabrication of a simple and cost-effective nanofilter for microfluidic chips. A microfluidic chip is fabricated using PDMS by standard photo-lithography technique. A nanofilter is synthesized using single-walled carbon nanotubes (SWNTs). Individual nanotubes are converted to carboxylated SWNTs (SWNT-COOH) through a chemical oxidation. The SWNT-COOHs are entangled each other by 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) as a linker. We use a PDMS mold to make a specific shape of the SWNT microblock required for the microfluidic chip. The nanofilter incorporated into the microfluidic chip can be used to concentrate biomolecules in a dilute solution or to separate the biomolecules based on size by microdialysis.
샘플 전처리 성능 향상을 위한 원형 포스트가 배열된 마이크로 채널의 최적화
권재성(Jae-Sung Kwon),안윤호(Yun-Ho An),맹주성(Joo-Sung Maeng),송시몬(Simon Song) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.11
Microfluidic chips are frequently used for the automation and miniaturization of the analytic processes of bio molecules such as DNA or proteins. Among physical phenomena occurred on a microfluidic chip, electrokinesis and dielectrophoresis are paid attention to as means to transport and control the bio molecules. To effectively utilize electrokinesis and dielectrophoresis, it is important to design the shape of a microchannel on a microfluidic chip. We investigate the effects of microchannel geometry with circular posts on electrokinesis and dielectrophoresis, and determine a microchannel geometry suitable for the clean-up or separation of bio molecules. In addition, we examine forces on particles exerted by electrokinesis and dielectrophoresis for various post diameters and spacings using an in-house CFD code. As a result, an optimal shape of a microchannel is suggested that is suitable for separating bio molecules with different surface charges.