Thermitomyces albuminosus powder의 랫드를 이용한 90일 경구투여독성시험

안민지(Min Ji An),허혜선(Hye Seon Heo),이지선(Ji Sun Lee),손혜영(Hye Young Son),임해옥(Hae Ok Lim),박강백(Gang Baek Park),이준헌(Joon Heun Lee),지재규(Jae Gyu Jee),박영철(Yeongchul Park) 한국생명과학회 2016 생명과학회지 Vol.26 No.10

계종버섯(Thermitomyces albuminosus)의 안정성을 입증하기 위하여 사료에 계종버섯의 분말을 5%, 2.5%, 1.25%, 0%를 혼합하여 90일 동안 랫드에 자유 급이 시켜 실험동물의 일반증상, 체중변화, 혈액학적 검사, 부검소견, 임상병리, 조직병리학적 검사의 결과를 토대로 독성을 평가하였다. 독성판정기준은 독성의 정도와 양상에 따라 weight-based classification (독성 강도에 따른 분류)로 분류 하였는데 비시험물질-유래 변화인 NOEL, 시험물질-유래 경미한 변화는 NOAEL, 시험물질-유래 중요한 변화는 LOAEL을 기준으로 나누었다. 시험결과, 수컷 좌, 우측 신장 중량이 용량의존적으로 증가하여 고용량군에서는 12%, 8% 증가 하였다. 하지만 임상, 조직병리학적 결과 독성으로 판단되는 소견이 관찰되지 않아 시험물질-유래 경미한 변화로 판단으로 분류하였다. 따라서 판정기준에 따라 수컷은 NOAEL 암컷은 NOEL이 식이함량 5%로 추정되지만 식약처고시에 따라 암수 모두 NOAEL이 식이함량의 5%로 추정할 수 있다. 따라서 계종버섯은 식품 또는 기능성식품으로의 개발에 문제가 없을 것으로 사료된다. Termitomyces albuminosus (Berk.) Heim is a well-known wild edible mushroom in the southern region of China. Novel cerebrosides, termed termitomycesphins, isolated from EtOH extract of T. albuminosus have shown significant neuritogenic activity. Neurotrophic factors have been targeted as potential therapeutic drugs for the treatment of neurodegenerative disorders. However, before expanding their applications to include food or therapeutic agents in Korea, a safety evaluation of T. albuminosus is needed. Herein, in a repeated-dose 90-day oral toxicity study, rats were exposed to a basal diet of powder ground from dried T. albuminosus at dose levels of 5%, 2.5%, 1.25%, and 0%. The following endpoints were evaluated: clinical observation, body weight, gross and microscopic pathology, clinical chemistry, and hematology. Significant dose-dependent increases in the weight of the left kidney were observed, possibly due to the test substance. Based on toxicity-decision criteria for minor compound-related changes (no observed adverse effect level [NOAEL] and no observed effect level [NOEL]), NOAEL was observed in male rats at a dose of 5% of dried T. albuminosus powder, and NOEl was observed in female rats at the same dose. The results point to the safety and potential use of T. albuminosus as a nontoxic neurotrophic factor.

IoT 기반 PM 수집 시스템과 예측 방법

안민지(Minji An),박찬영(Chanyeong Park),노현정(Hyeonjung Noh),윤재석(Jaeseok Yun) 한국정보과학회 2023 한국정보과학회 학술발표논문집 Vol.2023 No.6

학동기 어린이 주의력결핍 과잉행동장애에서 식이요인의 역할 규명

안민지(Minji An),안효진(Hyojin-An),황효정(Hwang-Hyo-Jeong),권호장(Ho-Jang Kwon),하미나(Mina Ha),홍윤철(Yun-Chul Hong),홍수종(Soo-Jong Hong),오세영(Se-Young Oh) 대한지역사회영양학회 2018 대한지역사회영양학회지 Vol.23 No.5

초등학생 대상의 STEAM프로그램 개발에 대한 국내 연구 동향 분석

안민지(An, Min-Jee) 한국디자인학회 2017 한국디자인학회 학술발표대회 논문집 Vol.2016 No.10

요가명상을 병행한 케이팝 댄스 프로그램이 여중생의 뇌유래신경영양인자와 주의력에 미치는 영향

안민지 ( Min-ji An ) 한국리듬운동학회 2021 한국리듬운동학회지 Vol.14 No.2

The purpose of this study is to provide basic data for the development of K-pop dance program that incorporates diverse activities, so that K-pop dance activities, which has currently received great attention from adolescent is helpful to students in growth stages from multiple aspect by investigating the effect of the 8-week K-pop dance program with yoga meditation on brain-derived neurotrophic factor and attention in female middle school students. In order to achieve the purpose of this study, 30 female students in the second year of H middle school were randomly selected and 10 students each were assigned to Yoga meditation+K-pop dance group(YKG), K-pop dance group(KG), and control group(CG). Brain-derived neurotrophic factor and attention were measured before and after 8 weeks of program intervention. The program was conducted for 70 minutes of 2 times a week for 8 weeks. Because the data were nor normally distributed, the Kruskal-Wallis test was used to verify the difference between the groups and the Mann-Whitney U test was used to post hoc test when there is significance between groups. The results were as follows; First, the changes of brain-derived neurotrophic factor by program intervention for 8 weeks were not significantly different between the three groups, but the width of decrease in the mean value showed large in the order Yoga meditation+K-pop dance group(YKG), K-pop dance group(KG) and control group(CG). Second, as a result of analyzing the attention difference between the groups by program intervention for 8 weeks, Yoga meditation+K-pop dance group(YKG) were significantly higher than K-pop dance group(KG) in the performance value P and continuity value C. In conclusion, compared to single K-pop dance activities, it was confirmed that K-pop dance program with yoga meditation had a more excellent effect on the development of brain functions and cognitive functions of adolescents.

백부자-대체 가능 한약재의 계종버섯에 대한 급성독성시험과 안전성등급화

안민지 ( Minji An ),박영철 ( Yeongchul Park ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.4

Objectives : Termitomyces albuminosus (Berk.) Heim is one of the famous wild edible mushrooms in the southern part of China. It is known that Termitomyces albuminosus, like Aconitum koreanum used in Korean traditional medicine, contains a kind of cerebroside, termitomycesphin, causing a pharmacologic effect on the neuron system. The pharmacologic effect of Termitomyces albuminosus can be used to possibly replace Aconitum koreanum. However, It needs to be certified as safe before it can be used. Here, a single-oral toxicity test and safety classification was conducted to obtain acute information of the toxicity of dried-Termitomyces albuminosus powder and to secure its safety in clinical applications. Methods : In order to calculate approximate lethal dose(ALD), test substance was orally administered to male and female SD-rat at dose levels of 5,000 and 0 (vehicle control) ㎎/㎏ (body weight). Based on the result of this toxicity, also the estimation of safety classification was calculated using the HED-based (human equivalent dose) MOS (margin of safety). Results : There were no mortalities, test substances treatment-related clinical signs, no changes in the body or organ weights, and no gross or histopathological findings at 14 days after treatment with test substance. Thus, the approximate lethal dose of dried-Termitomyces albuminosus powder was considered over 5,000 ㎎/㎏ in both female and male mice. Conclusions : Based on the limit dose, 5000 ㎎/㎏, it was estimated that dried-Termitomyces albuminosus powder is classified as “Specified class B” indicating that clinical dose is not limited to patients as safe as food.

STEAM 교육기반 박물관 체험전시에 게이미피케이션 적용이 교육에 미치는 영향

안민지(An, Min-Jee) 한국디자인학회 2016 한국디자인학회 학술발표대회 논문집 Vol.2016 No.5

요가를 활용한 K-POP 댄스 프로그램이 여자중학생의 수면의 질과 자각적 피로도에 미치는 영향

안민지(An, MinJi),김경래(Kim, KyeongLae) 학습자중심교과교육학회 2017 학습자중심교과교육연구 Vol.17 No.22

이 연구는 8주간의 요가를 활용한 K-POP 댄스 프로그램이 여자중학생의 수면의 질과 자각적 피로도에 미치는 영향을 분석하여, 학생들에게 실질적인 도움이 되는 신체활동으로서의 K-POP 댄스 프로그램 개발에 도움이 되고자 하였다. 이 연구의 목적을 달성하기 위해 충청남도 C에 소재한 H중학교 1, 2학년 여학생 30명을 임의로 선정한 후, 요가+K-POP 댄스 집단(A), K-POP 댄스 집단(B), 비교 집단(C)에 각각 10명씩 무선 배정하고 2017년 9월 수면의 질과 자각적 피로도를 사전측정하였고, 8주간의 프로그램 처치 후 2017년 11월 사후측정을 하였다. 프로그램은 주2회 70분씩 실시하였으며, 수면의 질에 대한 차이 검증은 공변량분석(ANCOVA), 자각적 피로도는 일원변량분석(one-way ANOVA)을 실시하였다. 실험의 주요 결과는 다음과 같다. 첫째, 수면의 질 총점에서A집단이 다른 두 집단에 비해 유의하게 낮은 점수가 나타나 수면의 질이 가장 높아졌음을 확인하였다. 둘째, 자각적 피로도의 총점수는 A집단의 평균이 다른 두 집단에 비해 낮은 점수를 나타내었으나 B집단과는 유의한 차이가 없는 것으로 나타났고 C집단에 비해서는 A집단과 B집단이 유의한 차이로 낮은 점수를 나타내어 자각적 피로도가 낮아졌음을 확인하였다. The purpose of this study was to analyze the effects of 8-week K-POP dance program using yoga on quality of sleep and self-reported fatigue of female middle school students to provide the basic data necessary for correcting and supplementing the K-POP dance program so that K-POP dance activities, which are currently receiving great attention from students, can be practically useful in many ways. In order to accomplish the purpose of this study, we selected 24 female second grade students in H-middle school in Chungcheongnam-do, were selected at random and the allocated 10 students each for the yoga + K-POP dance group(A), K-pop dance group(B) and comparison group and in September 2017, sleep quality and subjective fatigue were measured in advance, and after 8 weeks of program treatment, post-mortem measurements were taken in November 2017. The program was conducted for 70 minutes of 2 times a week for 8 weeks. ANCOVA was used to test for differences in quality of Sleep and one-way ANOVA was used for self-reported fatigue. The main results of the experiment are as follows. First, the group A showed significantly lower scores on quality of sleep than the other two groups, indicating that quality of sleep was the highest. Second, the total scores of subjective fatigue were lower in the A group than in the other two groups, but not significantly different from the B group. The group A and the group B showed lower scores than the group C, indicating that the self-reported fatigue was lower

리듬 점프 트레이닝이 여중생의 체력과 스트레스 및 자율신경계 활성도에 미치는 영향

박상균(Sang-Kyun Park),안민지(Min-Ji An),허승재(Seung-Jae Heo),이원찬(Won-Chan Lee) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.15

목적 이 연구는 12주간의 리듬 점프 트레이닝이 여중생의 체력과 스트레스 및 자율신경계 활성도에 미치는 영향을 규명하였다. 방법 이를 위하여 여자중학생 45명을 대상으로 리듬 점프 트레이닝 집단(RJG), 점프 트레이닝 집단(JG), 통제집단(CG)에 각각 15명씩 무선 배정하여 리듬 점프 트레이닝 전⋅후 체력과 스트레스, 자율신경계 활성도를 측정하였다. 리듬 점프 트레이닝 프로그램은 주 3회, 1일 45분, 총 12주간 실시하였으며, 연구 수행을 통해 얻어진 자료는 SPSS 20.0 통계 프로그램을 이용, 반복측정이원변량분석을 실시하였다. 결과 첫째, 체력 변인은 심폐지구력과 순발력에서 상호작용 효과가 나타났으며, RJG는 모든 변인에서 유의하게 증가하였고, JG는 심폐지구력과 유연성에서 유의한 증가가 나타났다. 둘째, 스트레스 변인은 누적 스트레스 지수, 신체적 스트레스, 심리적 스트레스 모든 변인에서 상호작용 효과가 나타났으며, RJG는 세 가지 변인 모두에서 유의하게 개선되었고, JG는 누적 스트레스 지수와 신체적 스트레스에서 유의하게 개선되었다. 셋째, 자율신경계 활성도 변인은 SDNN과 HRV index에서 상호작용 효과가 나타났으며, RJG는 HF, SDNN, HRV index에서 유의하게 증가하였고, JG는 SDNN에서 유의하게 증가하였다. 결론 점프 트레이닝은 여중생은 체력, 스트레스, 자율신경계 활성도에 부분적으로 긍정적인 영향이 있었으며, 리듬을 적용한 리듬 점프 트레이닝의 경우 그 효과가 더욱 크다는 것을 확인할 수 있었다. Objectives The purpose of this study was to analyze the influence of 12-week rhythm jump training on physical fitness, stress and autonomic nervous system activity in middle school girls. Methods The subjects were 45 middle school girls were selected at random and the allocated 15 students each for the rhythm jump training group(RJG), jump training group(JG) and control group(CG) and measured physical fitness, stress and autonomic nervous system activity before and after rhythm jump training. The program of rhythm jump training is implemented for 45 minutes of 3 times each week for 12 weeks. The data obtained from this study were analyzed using the SPSS 20.0 statistical program and two-way repeated ANOVA was used. Results First, physical fitness variables showed an interaction effect on cardiovascular endurance and power, RJG significantly increased in all variables, and JG showed significant increases in cardiovascular endurance and flexibility. Second, the stress variable showed an interactive effect in all variables of cumulative stress index, physical stress, and psychological stress, RJG was significantly improved in all three variables, and JG was significantly improved in cumulative stress index and physical stress. Third, autonomic nervous system activity variables showed an interactive effect in SDNN and HRV index, and RJG significantly increased in HF, SDNN and HRV index, and JG significantly increased in SDNN. Conclusions Jump training had a partial positive effect on physical fitness, stress, and autonomic nervous system activity in middle school girls, and it was confirmed that the effect was greater in the case of rhythm jump training to which rhythm was applied.

스텝박스 운동이 비만 여중생의 건강 관련 체력, 골밀도 및 신체적 자기개념에 미치는 영향

이성준(Seong-Joon Lee),안민지(Min-Ji An),김경래(Kyeong-Lae Kim) 한국교원대학교 뇌기반교육연구소 2019 Brain, Digital, & Learning Vol.9 No.4

The purpose of this study is to provide basic data necessary for the development of an exercise program for the prevention and management of obese female students at school by investigating the effect of the participation in Step Box Exercise for 12 weeks on the Health Related Fitness, Bone Mineral Density and Physical self-concept of obese female middle school students. In order to accomplish the purpose of this study, 24 1st and 2nd grade obese female students at H Middle School located in C City, Chungcheongnam-do, were selected at random and the allocated 10 students each for the exercise group and comparison group and measured Health Related Fitness, Bone Mineral Density and Physical self-concept before and after the participation in Step Box Exercise. The data obtained from this study were analyzed using the SPSS 20.0 statistical program and Two-way repeated ANOVA was used to measure the interaction effect of each variable. The conclusions obtained through this study are as follows. First, flexibility showed significant differences in group, cardiovascular endurance showed significant differences in timing, and muscle endurance, cardiovascular endurance, body fat percentage showed interaction effects in the group and time. Second, in Bone Mineral Density, there was improvement of average value in exercise group, but no significant difference in group, timing and interaction effects. Third, in physical self-concept, health, physical activity, self-esteem and endurance showed significant differences in group, body fat, physical activity, self esteem, body overall showed significant differences in timing, and sports competence, body fat, health, physical activity, self esteem, endurance, body overall showed interaction effects in the group and time.