조선 능제(陵制)의 회격(灰隔) 조성방법

안경호(An Kyoung-ho) 한국학중앙연구원 2009 한국학 Vol.32 No.3

해양생태독성 평가용 표준시험생물로서 섬모충류 Mesodinium rubrum에 대한 연구

안경호 ( Kyoung Ho An ),박경수 ( Gyung Soo Park ),이승민 ( Seung Min Lee ) 한국환경과학회 2011 한국환경과학회지 Vol.20 No.9

The mixotrophic marine ciliate Mesodinium rubrum possesses a highly modified algal endosymbiont as a nutrition source for the species. Accordingly, we assumed that the species can reflect the ecotoxicity on marine producer (as phytoplankton) and consumer (as zooplankton) both. A series of experiments were conducted to identify the potential of the species as a standard test species for marine ecotoxicological study. The comparison of species sensitivity on reference toxic materials was made using potassium dichromate for phytoplankton and copper chloride for zooplankton. The ciliate revealed the highest sensitivity on both reference materials among the seven test species including phytoplankton, benthic copepod and rotifer species. The toxicity end point of the species was 72hr-EC50=1.52 mg/L (as potassium dichromate) estimated by population growth inhibition (PGI), which is more sensitive than the most sensitive phytoplankton Skeletonema costatum (72hr-EC50=3.05 mg/L). As comparison to rotifer, it also revealed higher sensitivity on copper chloride; 72hr-EC50=0.38 mg/L for ciliate and 48hr-EC50=0.48 mg/L for rotifer. Also, the elutriate toxicity test of various ocean disposal wastes were conducted to identify the potential of ciliate toxicity test application using industrial waste sludges. The toxicity of leather processing waste sludge was highest on the ciliate, followed by dyeing waste sludge and dye production waste sludge as an increasing order of toxicity. 72h-EC50 of ciliate PGI test was 1.83% and that of S. costatum 3.84% for leather waste sludge which showed highest toxicity. The toxicity test results also revealed that the highest sensitivity was observed on ciliate species on ocean disposed sludge wastes. Also, ciliate toxicity test well discriminated the degree of toxicity between sludge sources; 72h-EC50 values were 1.83% for leather processing waste sludge, 16.75% for dye production waste sludge and 27.75% for textile production waste sludge. Even the laboratory culture methods of the species were not generally established yet, the species has high potential as the standard test species for marine toxicity test in terms of the dual reflection of phyto- and zooplankton toxicity from single test, sensitivity and test replicability.

세종대왕 초장지(舊 英陵)에 대한 재론(再論)

안경호(An Kyoung-ho) 한국학중앙연구원 2008 한국학 Vol.31 No.2

선천성 십이지장폐쇄

안경호(Kyoung Ho Ahn),최규형(Kyu Hyung Choe),이용배(Yong Bae Lee) 대한외과학회 1998 Annals of Surgical Treatment and Research Vol.54 No.3

원추각막에 있어서 각막이식수술후 발생한 마비성 동공에 대한 임상적 고찰

안경호(Kyoung Ho Ahn),정성근(Sung Kun Chung),이상욱(Sang Wook Rhee) 대한안과학회 1994 대한안과학회지 Vol.35 No.3

황해 공동 관리시스템 기반 조성을 위한 한.중 황해환경공동조사

허승,안경호,박승윤,박종수,강영실,손재경,김평중,김형철,황운기,이승민,황학진,최용석,고병설,방현우,Heo, Seung,An, Kyoung-Ho,Park, Soung-Yun,Park, Jong-Soo,Kang, Young-Shil,Shon, Jaek-Young,Kim, Pyoung-Joong,Kim, Hyung-Chul,Hwang, Woon-Ki,Lee, S 해양환경안전학회 2009 해양환경안전학회지 Vol.15 No.3

한 중 황해환경공동조사 연구는 1993년 11월 한 중 환경협력에 기초하여 1995년 5월 한 중 환경협력공동위원회에서 채택되어, 1997년부터 매년 1회씩 한국과 중국이 황해에 대한 공동조사 및 분석을 격년별로 주관하여 실시하고 있다. 본 연구는 한 중 양국이 황해를 대상으로 양국 과학자가 공동으로 승선조사 및 분석을 실시하는 연구로서, 황해에서 해양환경의 특성과 잠재적 오염압력을 평가하고, 과학적이며 체계적인 데이터베이스를 구축하며, 황해 환경에 대하여 한 중 양국이 국가적 차원에서 공동으로 관리할 수 있는 기반을 마련한다는 데 큰 의의가 있다. 또한 황해 오염현상의 공동연구를 통한 한 중 간 해양환경 협력 기반을 구축하고, 황해에 대한 과학적이며 체계적인 해양학적 자료의 축적 및 황해환경 보전을 위한 자료 제공 등 한 중 간 창해 공동관리 시스템 기반을 조성하기 위한 것이다. 지금까지 조사된 결과, 조사해역의 해양환경은 양호한 상태로서 해역 생활환경 수질기준 I등급을 나타내었다. 그러나 매년 황해의 COD 값이 점차 증가하는 경향을 보이고 있으며, 최근 양국 연안지역에서의 인구밀집과 산업화로 인하여 해양환경오염이 가중되고 있는 실정으로 오염 부하량이 지속적으로 증가할 것으로 예상된다. 지금까지 실시된 한 중 황해환경 공동조사는 연 1회 실시되고 있어, 황해의 다양한 해양환경 특성을 상세히 밝히기는 어려우나, 한 중 양국 간 황해 관련 각종 환경정책을 입안하는 데 필요한 기초 자료를 산출하는 데 있어 중요한 역할을 하고 있다. 따라서 양국은 앞으로 조사 횟수, 조사해역 및 조사항목을 연차적으로 확대할 계획이다. The Yellow Sea is extremely important to the economy and to the health and well-being of surrounding countries, Korea and China. Recently, the Yellow Sea is under constantly increasing threat of degradation due to the increase of environmental pollution and over-fishing. The governments of Korea and China have been aware of the importance of the Yellow Sea and reached an Environmental Agreement between Korea and China at the governmental level(November, 1993) According to this environmental agreement the Yellow Sea Environmental Cooperative Research between Korea and China has been undertaken since 1997. The joint cruise had been conducted once a year at 33 stations in the 4 lines of the Yellow Sea where the 9 stations of the D line was newly added in the 7th cruise in 2003. The samples were analyzed by scientists of both countries at the WSFRI, Korea and the OEMNC of the SEPA, China in turn, the annual report has been published every year during 1998-2008. The scientific efforts to fix the cruise time in October and to extend research frequency, as twice a year, should be considered, and this requires the governmental supports such as research funds and other related administrational assistance on both sides. Finally, scientists should also pay a concentrated attention to standardize the analytical methods including quality control and to improve this Yellow Sea research as one of the most representative international projects in the Yellow Sea where sharing additional informations available, if exist of dumping sites and material content, and of the freshwater quality will be of great help to broaden the output of this joint research project.

Effects of 4-Nonylphenol on the Induction of Plasma Vitellogenin (VTG), Alkaline-Labile Protein Phosphorus (ALPP), Calcium (Ca), Glutamate Pyruvate Transaminase (GPT) and Hepatosomatic Index (HSI) in the Immature Rockfish, Sebastes schlegeli

황운기,안경호,진현국,박승윤,김평중,이승민,Hwang, Un-Ki,An, Kyoung-Ho,Jin, Hyoun-Kook,Park, Seung-Youn,Kim, Pyoung-Joong,Lee, Seung-Min The Korean Society of Environmental Toxicology 2007 환경독성보건학회지 Vol.22 No.4

4-nonylphenol (4-NP)이 해산어류인 조피볼락, Sebastes schlegeli의 혈장 vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), calcium(Ca), glutamate pyruvate transaminase (GPT) 및 hepatosomatic index (HSI)에 미치는 영향을 조사하였다. 실험어에 3일간격으로 $estradiol-17{\beta}$ ($E_2$, 5 mg/kg B.W.) 또는 4-NP(0, 10, 50, 100및 200 mg/kg B.W.)을 복강에 2번 주사한 후, 7일째에 채혈과 적출을 통해 혈장과 간장을 수집해 분석이 실시되었다. 대조 실험어에는 용매로 사용된 70% 에탄올만이 투여되었다. $E_2$ 투여 실험어의 혈장 단백질을 전기 영동상으로 분석한 결과 약 170 kDa의 위치에서 짙은 VTG 밴드가 관찰되었으나, 용매만 투여한 대조 실험어의 혈장에서는 동일 밴드가 관찰되지 않았다. 4-NP 투여한 모든 실험어의 혈장 단백질에서는 $E_2$ 투여 실험어와 동일한 VTG 밴드가 관찰되었다. 혈장 ALPP와 Ca 농도도 4-NP 투여 실험어에서 $E_2$ 투여 실험어와 유사하게 증가하였으며, 이들 농도 변화는 VTG 합성과 더불어 증가하는 경향을 나타냈다. 혈장 전위효소인 GPT와 HSI도 $E_2$ 투여 실험어와 유사하게 4-NP가 투여된 모든 실험어에서 급격히 증가하였다. 이상의 결과로부터 연안생태계 내에서 서식하는 어류가 4-NP과 같은 내분비 장애물질(Endocrine Disrupting compounds, EDCs)에 의해 영향을 받는지를 규명하기 위한 생물학적 지표로서 VTG와 더불어 혈장 ALPP와 Ca이 사용가능 할 것으로 판단된다. 또한, 조피볼락과 같은 해산어가 EDCs에 노출되어 VTG가 합성될 때 간장 기능의 손상으로 혈장 전위효소인 GPT가 일시적으로 종가하고 간장도 비대해져 HSI가 높아지는 것으로 판단된다.

한국 태안군 진산리 저서미세조류의 계절별 종조성 및 생물량 변화

박서경 ( Seo Kyoung Park ),김보연 ( Bo Yeon Kim ),최한길 ( Han Gil Choi ),오정순 ( Joung Soon Oh ),정상옥 ( Sang Ok Chung ),안경호 ( Kyoung Ho An ),박광재 ( Kwang Jae Park ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.2

Seasonal species composition and biomass of microphytobenthos were examined at the intertidal flats of Jinsanri. Taean, Korea. from April to December 2011. In total, 121 species were identified, including 109 diatoms, 5 blue-green algae, and 5 dinoflagellates. Seasonal variation in species number ranged from 49 to 56 species (minimum in October and maximal in April), while vertical variation across three different shore levels ranged from 64 to 77 species with maximum at high and minimum at low shore. Species diversity ranged from 3.22 to 3.42 seasonally and from 3.50 to 3.17 across vertical shore levels. Seasonal average values of the normalized difference vegetation index and the vegetation index were 0.06 and 1.14, respectively. Chlorophyll a concentrations were 66.00-120.73 mg/m2, with a seasonal minimum and maximum in July and October. respectively, and a vertical shore maximum of 137.35 mg/Itt2 at mid shore. Phaeopigmcnt concentrations peaked in October (50.78 mg/rn2) and at high shore (61.58 mg/ rn2) and were lowest in December (22.53 mg/rn2) and at low shore (15.28 mg/rn2). The number of microphytobenthos ranged from 78,556 to 287.898 cells/cm2. reaching a maximum in April and a minimum in July. The diatoms Paralia sulcata, Navicula sp.. and Delphines surirella were dominant at all tidal levels over the study period. Dinoflagellates were dominant in July at low shore, and blue-green algae were abundant between July and October at mid shore. In conclusion, species composition and primary production of microphytobenthos exhibited clear seasonal and vertical patterns.

Cochlodinium polykrikoides(Dinophyceae)에서 분비되는 유기물질의 특성

강양순,권정노,안경호,Kang, Yang-Soon,Kwon, Jung-No,An, Kyoung-Ho 한국조류학회(藻類) 2006 ALGAE Vol.21 No.2

Organic substances are released from phytoplankton cells during all phases of growth. The type and amounts of organic substance excreted and the effects of nutrient limitation are often highly species-specific. Dinoflagellate, Cochlodinium polykrikoides grown in batch culture produced an exopolysaccharide. Exopolysaccharide and intracellular polysaccharide concentrations increased as C. polykrikoides cultures progressed from exponential phase, through stationary phase, to declining phase. In the exponential phase, the concentration of exopolysaccharide was relatively low, but in the stationary phase, it showed a rapid increase which seemed to coincide with the depletion of nitrate from the medium. Of the 20 amino acids analyzed, proline dominated in the organic matter of all cultures ranging from 48.2 to 79.9 nmol L?1, and constituting the 20-90% of total amino acids, and followed by histamine varying from 0.7 to 47.5 nmol L?1. Leucine and cysteine were also abundant in the stationary phase. The release rates of exopolysaccharide and intracellualr polysaccharide were higher the end of stationary phase than in the exponential phase. Exopolysaccharide concentration per cell was more than two times higher during the end of stationary phase than that in exponential phase. C. polykrikoides produced extracellular polysaccharide at a rate of 47.04 pg cell?1 day?1.

황해의 해양환경 모니터링 시스템

허승,박종수,안경호,이윤,최옥인,임동현,황운기,이승민,김평중,방현우,Heo, Seung,Park, Jong-Soo,An, Kyoung-Ho,Lee, Yoon,Choi, Ok-In,Lim, Dong-Hyun,Hwang, Woon-Ki,Lee, Seung-Min,Kim, Pyoung-Joong,Bang, Hyun-Woo 해양환경안전학회 2010 해양환경안전학회지 Vol.16 No.3

황해의 해양환경, 어업자원 및 증양식에 대한 연구 및 개발을 담당하고 있는 국립수산과학원 서해수산연구소에서 해양생태계 보전의 일환으로 황해의 해양환경에 대한 다양한 모니터링을 실시하고 있다. 현재 서해수산연구소가 황해에서 수행 중인 모니터링은 연안정지관측, 서해해양조사, 서해어장환경모니터링, 국가해양환경측정망, 유해적조모니터링, 한중황해환경모니터링조사 및 해파리모니터링 등이 있다. 이러한 모니터링 결과들은 중요 어족의 어장형성 해역에 대한 기초자료를 제공하여 연근해 어업생산성을 제고하고, 기후변동에 따른 향후 수산자원 변동 예측을 위한 기초자료로 활용되고, 한반도 주변해역의 체계적인 해양과학자료를 제공하여 수산업, 해양환경보전, 해양레저 등 한반도 주변해역의 부가가치 창출에 기여하고 있으며, 연안어장의 지속적 생산을 유지하기 위한 어장환경 관리 및 보전정책 수립을 위한 국가 기본 자료로 활용하고 있다. The West Sea Fisheries Research Institute of National Fisheries Research and Development Institute which is in charge of research on marine environment, fisheries resources and aquaculture carries out various monitoring projects with an aim of marine ecosystem conservation. The monitoring projects include costal oceanographic observation, serial oceanographic observation, fishing ground monitoring, national marine environmental monitoring, harmful algal bloom monitoring, Korea-China joint monitoring on the Yellow Sea and jellyfish monitoring. The monitoring produces basic data on fishing ground locations of main fishery species to improve fishery productivity. The data are also used to estimate variations in fisheries resources caused by climate change and to set up the policy for creating economic value from fishery, marine environmental conservation and marine leisure activities and conserving/controlling the marine environment for the sustainable production in the fishing ground.