인슐린 비의존형 당뇨병 환자에서 MTHFR 유전자 다형성과 당뇨병성 합병증과의 관계

신종성(Jong Sung Shin),오광식(Kwang Sik Oh),권순길(Soon Kil Kwon),지정훈(Jeong Hoon Ji),이태원(Tae Won Lee),박영선(Young Sun Park),김미성(Mi Sung Kim),궁성수(Seong Su Koong),오태근(Tae Geun Oh) 대한내과학회 2001 대한내과학회지 Vol.61 No.4

N/A Background: Diabetic nephropathy develops in 20-30% of patients with non-insulin dependent diabetes mellitus (NIDDM). Poor glycemic control, hypertension and duration of diabetes are known as risk fact ors for the development of diabetic nephropathy and there is high prevalence of diabetic nephropathy in the patients who have familial history of diabetic nephropathy, so it has been assumed that genetic fact or is associated with the background of its occurrences. Recently it has been observed that a cytosine to thymidine substitution of the methylenetetrahydrofolate reductase (MTHFR) gene at nucleotide 677 (C677T) was related to diabetic nephropathy in patients with NIDDM and MTHFR gene polymorphism was also known to predispose to vascular disease. This study was performed to investigate whether MTHFR gene polymorphism is associated with the development of diabetic nephropathy and macrovascular disease in NIDDM patients. Methods: The study population consisted of 243 NIDDM patients (duration≥10 years). Nephropathy was defined by 24 hour urinary protein excretion of more than 500 mg. The MTHFR gene fragment was extracted using the polymerase chain reaction. The presence of the mutation was identified by HinfI digestion, which cuts at the mutation site, followed by 2.5% metaphore agarose electrophoresis and ethidium bromide staining. Statistical differences in genotype distribution and allele frequencies among the groups were assessed by the chi-square test. Results: There was no difference in clinical characteristics except the prevalence of hypertension and diabetic retinopathy between nephropathy group and non-nephropathy group. The data do not show any difference of genotype distribution or allele frequencies between patients with or without diabetic nephropathy and macrovascular disease Conclusion: With the above results, it is assumed that there are no significant relationships among MTHFR gene polymorphism, diabetic nephropathy, and macrovascular disease. (Korean J Med 61:391-398, 2001)

백서 심근경색모델에서 시간경과와 경색의 크기에 따른 심자도의 변화

김미성(Mi Sung Kim),박영선(Young Sun Park),권순길(Sun Gil Kwon),지정훈(Jeong Hoon Ji),신종성(Jong Sung Shin),오광식(Kwang Sik Oh),양용모(Yong Mo Yang),연태진(Tae Jin Youn),김동운(Dong Woon Kim),조명찬(Myeong Chan Cho),이용호(Yong Ho 대한내과학회 2002 대한내과학회지 Vol.62 No.1

N/A Background: Magnetocardiogram (MCG), which records the changes of magnetic fields generated by the heart`s electrical activity, theoritically can provide unique data for clinical application. To date, MCG has been investigated only at a single time point after myocardial infarction (MI) with severe left ventricular dysfunction in rats. The purpose of the present study was to investigate sequential changes of MCG after MI and to evaluate effects of infarct size on MCG. Methods: Acute MI were induced by the permanent ligation of left coronary artery in 22 rats. Magnetic fields were recorded just above a rat with Nb Superconducting Quantum Interference Device (SQUID) gradiometer inside a magnetically shielded room. MCG was measured before and immediately after surgery and it was subsequently recorded at the time points of 1, 4 and 6 hours post operatively. MCG was also measured at 1, 3, 7 and 21 days after surgery. Results: Elevation of ST segment and appearance of pathological Q wave on the MCG were evident immediately after the ligation of coronary artery and persisted to 6 hours after MI. On MCG, ST segment was depressed and T wave was inverted from 1 day after MI. In rats with small-and moderate- sized MI (infarct size〈30%), ST depression returned to near the isoelectric level and Q wave disappeared from 7 days after MI. However, ST depression and Q wave were still present in rats with larger infarct (infarct size≥30%). Conclusion: Evolutional changes of MCG were well-recognized up to 21 days after MI. Furthermore, the infarct size can be expressed by the extent of Q wave and ST segment depression on MCG. Taken together, these data indicate that MCG is a helpful modality for the diagnosis, evaluation of infarct size and follow up after MI.(Korean J Med 62:42-48, 2002)

웹사이트 구축과 활용에 의한 효율적인 선교전략

김희완 ( Hee Wan Kim ),신종성 ( Jong Sung Shin ),이석민 ( Suk Min Lee ),장용선 ( Yong Seon Jang ),정진수 ( Jin Soo Jeong ) 글로벌기독교세계관학회 2011 학문과기독교세계관 Vol.2 No.-

The purpose of this study is to introduce the website constructed by Sahmyook University church and to suggest that the practical use of the website is one of the efficient evangelism strategies. First of all, right information on the adventist church and good news of the Bible can be given to the majority of non-adventist students on campus through the website. Furthermore, various kinds of worships are able to be known to those who want to attend one of the worship services and the Bible search is also one of the services available in this website. Especially, through the Sahmyook University church website, more than 800 worship services can be broadcasted, shared and watched by other people in local churches for which there are no pastors, those in the hospital, the handicapped people, and all the people around the country and the world, fulfilling the God`s command of "being my witnesses in Jerusalem and in all Judea and Samaria and to the end of the earth."(Acts 1:8)

XML / JAVA를 이용한 상품 카달로그 시스템 구현

이경호,신종성,박두순 순천향대학교 부설 산업기술연구소 2002 순천향 산업기술연구소논문집 Vol.8 No.1

A catalog system is defined as an electronic commerce entity that provides a common web-based storefront to a group of merchants. Customers access the "catalog system" web site to browse catalogs of merchant companies that deliver goods and services. This enables customers to search for goods, compare prices at a central location instead of visiting multiple dislocated web sites. The catalog system model is attractive to small business for which the benefits of maintaining an individual web site may never justify the overhead. The catalog system maintains a database containing catalog information for each merchant. A existing catalog system don's have a standard catalog system. This paper presents an implementation model, that uses Extensible Markup Language(XML) and JAVA to overcome the standard problem. We propose goods catalog system with XML, and JAVA.

Escherichia coli O157:H7 감염의 신속한 분자생물학적 진단

신형식,오태근,신종성,지정훈,손보라,신경섭 대한감염학회 2001 감염 Vol.33 No.2

Background : Sorbitol fermenting Escherichia coli O157 were reported. And E. coli O157 : H7 produce various Shiga toxin (Stx) such as Stx1, Stx2, or variants of Stx2. In this study, we tried to establish laboratory methods that detect E. coli O157 : H7 quickly and precisely by analyzing sensitivity of colony hybridization test and PCR technique. Methods : Stx1-producing E. coli ATCC 43890, Stx2-producing E. coli ATCC 43889, and Stx2vha- producing E. coli ATCC 51435 were tested. Three strains of E. coli were diluted with 0.1 g of diarrheal stools from 107 CFU to 101 CFU respectively. The stool samples were incubated overnight in MacConkey agar plates. A mean of 63 colonies were hybridized by stx1- and stx2-specific oligonucleotide probes. PCR for stx1 gene and stx2 gene was done after overnight-incubation of stool samples in the LB broth with vancomycin (6 ug/mL). Positive colonies by colony hybridization were confirmed by PCR for stx1 gene and stx2 gene. Results : Colony hybridization test could detect Stx1-producing E. coli at 103 CFU per 0.1 g of stool, Stx2-producing E. coli at 105 CFU per 0.1 g of stool, and Stx2vha-producing E, coli at 104 CFU per 0.1 g of stool. PCR technique after enrichment in LB broth with vancomycin (6 ug/mL) could detect stx1-, stx2-, and stx2vha-containing E. coli at 10 CFU per 0.1 g of stool respectively. Conclusion : A combination of colony hybridization and PCR after enrichment in broth with van-comycin (6 ug/mL) is usefu1 for the rapid and precise diagnosis of infections of Shiga toxin-producing E. coli O157 : H7.(Korean J Infect Dis 33:97∼103,2001)

알코올성 간경변 환자에서 위대망 정맥류 출혈로 인한 자발성 혈복강 1 예

채희복,윤세진,박선미,성노현,배일헌,임정묵,장이찬,최기원,신종성,지정훈 대한간학회 2001 Clinical and Molecular Hepatology(대한간학회지) Vol.7 No.3

Portal hypertension often leads to the development of several collateral vessels that shunt blood flow from the portal to the systemic circulation. The rupture of intra-abdominal varix is an unusual complication of portal hypertension that can lead to life-threatening hemoperitoneum of the patient is herrmdynamically unstable due to massive intra-abdominal bleeding, exploratory laparotomy should be performed on the patient. There are several reported cases of intra-abdominal variceal bleeding such as paraumbilical varix, the varix from the small intestine and proximal colon etc. Spontaneous hemoperitoneum caused by the rupture of gastroepiploic vein varix, however, has not been repzted in Korm, to the best of our knowledge. We wiU discuss a patient with portal hypnsion due to liver cirrhosis who pnmented with acute intra-abdominal bleeding. During the laparotomy, he was found to have a rupture of the gastnxpiploic vein. 'Ihe vessel was ligated, and the patient recovered uneventfully by operative variceal ligation. (Korean J Hepatol 2001;7:336-340)