돼지 폐렴병소로부터 분리한 Pasteurella multocida에 관한 연구 : 항균제 감수성, plasmid profile 및 toxA 유전자 분포

신나리,박주연,박용호,유한상,Shin, Na-ri,Park, Joo-youn,Park, Yong-ho,Yoo, Han-sang 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.6

Antimicrobial susceptibility, plasmid profiles and distribution of toxA gene were investigated in Pasteurella multocida isolated from pneumonic lung lesions of swine. The bacteria were highly susceptible to norfloxacin, cabenicillin, enrofloxacin and chloramphenicol, but resistant to colistin, sulfamethoxawle/trimethoprime, bacitracin, streptomycin. Sixty percentage of the isolates was resistant more than 2 drugs used in this experiment and 21 strains (23.6%) were resistant more than 5 drugs. This phenomenon meant that they had highly multi-drugs resistance. In the analysis of plasmid profiles, nineteen strains (47.5%) of 40 P multocida isolates harbored plasmids, ranging from 53.3kb to 2.49kb in size and the plasmid profiles could be classified into 5 groups. However, there was no relationship between the size and the profile of plasmid and the resistance pattern of antimicrobial agents. Thirty strains of 39 P multocida isolates (77%) investigated by PCR harbored toxA gene. This result suggested involvement of the ToxA protein expressed from the gene in pneumonic pasteurellosis of swine.

난황항체를 이용한 돼지 호흡기 질병 방제 관한 연구 I. Bordetella bronchiseptica, Pasteurella multocida 및 Actinobacillus pleuropneumoniae의 주요 면역원 분석 및 IgY의 생산

신나리,김종만,유한상,Shin, Na-ri,Kim, Jong-man,Yoo, Han-sang 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.3

Swine respiratory diseases have induced severe economic losses in swine industry worldwide. Several methods have been developed and applied to prevent and control the disease. However, those are still problematic in swine industry. Recently, the use of egg yolk antibodies with several advantages was introduced and applied to control diseases in animal as well as human. As the first step of the use of egg yolk antibodies in the control of the swine respiratory diseases, we investigated the immunogens of the causative agensts of the diseases and immune response in egg yolk of hens immunized with them. Bacterial antigens prepared from Bordetella bronchiseptica, Pasteurella multocida 3A and 4D, and Actinobacillus pleuropneumaniae serotype 2 and 5 were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Western blot and toxicity test in mice. The antigens were injected into laying hens in order to produce antibodies against them in egg yolk. After chickens were immunized three times in 2 weeks interval, the profile of antibody production was examined by ELISA. The production of antibody in egg yolk was started in 2 weeks after the first injection, reached peak in 6-8 weeks and maintained until 12 weeks. Of two adjuvants used in this study, ISA70 was more effective than aluminum hydroxide gel in enhancing immunogenecity, laying rates and safety in hens. These results suggested that egg yolk antibodies could be a good source for production of antibodies specific to pathogenic bacteria inducing respiratory diseases of swine.

돼지 위축성 비염, 파스튜렐라성 폐렴 및 흉막폐렴 원인균의 주요 항원에 대한 IgG 와 IgY 의 상관 관계 분석

신나리,김종만,유한상,Shin, Na-Ri,Kim, Jong-Man,Yoo, Han-Sang 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.3

Swine respiratory diseases have induced severe economic lasses in swine industry worldwide. Therefore, several methods have been made and applied to prevent and control the diseases. However, these methods still have a problem and also induce side effects. Recently, the use of egg yolk antibody was introduced to control and prevent the diseases as one of new trials. As a study of using egg yolk antibody, antibody titers against several different antigens of major pathogens in swine respiratory diseases were compared in egg yolk and serum of hens immunized with those antigens. The titers were measured by ELISA using the antigens as coating antigens. The relationship in antibody titers between egg yolk and serum were identified by analysis of variance for linear regression. Almost of antigens used in this study showed the high relationship in antibody titers between egg yolk and serum (r = 0.87 ~ 0.93) even though the relationship in antibody titers against P. multocida A:3 IROMP was slightly low (r = 0.74)(P<0.01). These results indicated that antibody titer in egg yolk could be useful to predict the titer in serum of chicken.

난황항체를 이용한 돼지 호흡기 질병 방제에 관한 연구 II. 면역된 산란계로부터 생산된 난황항체의 특이성 분석

신나리,김종만,최인수,유한상,Shin, Na-ri,Kim, Jong-man,Choi, In-soo,Yoo, Han sang 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2

Although several methods have been developed and applied to control swine respiratory diseases, the disease induces severe economic impact to swine industry worldwide. As one of the new trials, application of egg yolk antibody(IgY) was attempted for the purposes and immune response in sera and egg yolk was analysed with ELISA in previous study. In this study, immunological specificity of the IgY was analysed by Western blot analysis. In the analysis of causative agents of atrophic rhinits, B bronchiseptica and P multocida 4D, proteins of 33, 40, 43, 67 and 141 kDa were specifically reacted with IgY Also, 40 and 110 kDa proteins were identified as the major immunogens in P multocida 3A. In A pleuropneumoniae serotypes 2 and 5, 40 kDa and 47 kDa proteins were found to be the major reactive ones. These results suggested that egg yolk antibodies from immunized hens was specific with antigens injected into hens and partially purified antigens, outer membrane proteins and dermonecrotic toxin, were more effective than bacterin for the production of specific antibody.

난황항체를 이용한 돼지 호흡기 질병 방제에 관한 연구 III. 마우스에서의 방어 효과

신나리,김종만,유한상,Shin, Na-Ri,Kim, Jong-man,Yoo, Han-Sang 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3

As an attempt to control respiratory disease in swine, specific immunologloblin Ys(IgYs) against bacterial pathogens of the diseases were produced and specificity of the IgYs was analysed with Western blot in the previous studies. In this studies, the immunoprotective effects of produced IgY were evaluated in the mice. Mice were challenged with minimal lethal doses of P multocida 3A and 4D, B bronchiseptica and A pleuropneumoniae serotype 2 after intraperitoneal administration of IgY and the protectivity by IgY was dose-dependent at the concentration of 100, 200 and 400 mg/ml. These results suggested that IgY could be a potential immunoprophylactic candidates against those pathogens in swine and apply and effective source of passive immunity for other disease in animals.

중합방법의 차이가 PU/organoclay 나노복합체의 미세 구조 및 열적/기계적 특성에 미치는 영향

신나리,권병철,박기호,이한섭,Shin, Na-Ri,Kwon, Byung-Chol,Park, Ki-Ho,Lee, Han-Sup 한국섬유공학회 2007 한국섬유공학회지 Vol.44 No.1

Two different polymerization methods were used to synthesize the PU/organoclay nanocomposite by either 'one step addition' or 'two step addition' method. In two step addition method, the modified clay was reacted with isocyanate-terminated prepolymer to anchor the polymer chains to the silicate layers before the chain extension reaction occurred. On the other hand, the modified clay and chain extender were added simultaneously in the one step addition method. The effect of polymerization method on the exfoliated/intercalated structure of silicate layers as well as on the microphase separated structure of PU matrix has been studied with FTIR, SAXS and TEM methods. The PU/organoclay nanocomposite prepared with one step addition reaction showed better exfoliated structure which is effectively retarding the micro-phase separation process of PU. However, compared with two step addition one, one step addition method was more effective to enhance the initial modulus and tensile strength of the nanocomposites. The role of modified clay for the segmental orientation process was also studied by infrared dichroism method. The orientation process was hindered by the silicate layers and its effect was more pronounced in the nanocomposite by one step addition method.

해체주의 특성을 응용한 아트마스크 작품 연구 -프랭크 게리의 작품을 중심으로-

신나리 ( Na Ri Shin ),문윤경 ( Yun Kyeong Mun ) 한국미용학회 2016 한국미용학회지 Vol.22 No.3

De-constructivism has influences in various fields of literature, art, architecture, fashion, and cosmetics, based on a philosophical theory to overcome limits by de-constructing and de-building the existing things, pursuing new changes and showing possibilities of interpretation of new designs by encounter with diverse fields. However, it still lacks for discussing artistic values in make-up. The contemporary make-up requires unique designs as their own artistic values and reflecting the artists` ideas and social backgrounds at that time, transcending just a pursuit of beauty. This study aims at overcoming limits of make-up arts, suggesting directions for new make-up designs with diverse attempts, and extending the field by applying figurativeness of Frank Gehry, one of architects who represent Deconstructivism. This study shows that an artistic trend, architecture and make-up collaborated and represent a new fusion art and provides an opportunity to establish a new genre as well as possibility of infinite make-up designs beyond the scope of expressions and limits of materials and everlasting exhibition of make-up through art-mask works.

KASP 마커를 이용한 호박 유전자원의 다양성 분석 연구

신나리(Na-Ri Shin),강한솔(Han Sol Kang),정우진(Woo Jin Jeong),이오흠(Oh Hum Lee),배익현(Ik Hyun Bae),최윤서(Yun Seo Choi) 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.5

다양한 지면 보행 훈련이 뇌졸중 환자의 보행 및 균형 능력에 미치는 영향

신나리(Shin Na-Ri),우영근(Woo Young-Geun),유영열(You Young-Youl) 대한치료과학회 2015 대한치료과학회지 Vol.7 No.2

Objective: The research identifies the effects of various stimulation gait training on gait capacity for stroke patients. Method: 40 stroke patients, 20 various stimulation gait training group(VSGT) and 20 Ground gait training(GGT) were randomly assigned. Both group had gait training for 8 weeks, 5 times a week for 30 minutes. VSGT had gait training with mixed various obstacles over a surface and instability ground for 30 minutes, GGT trained with general thera py environment for 30 minutes. To exclude exercise effect, each patient allowed to walk as gait habbit, and induced to get enough rest. Spatiotemporal elements of Gait measured through Zebris FDM-T Treadmill, function and capacity mesured by FMA-L, K-BBS, TUG, 10mWT. General characteristics of the patients were subjected to normality test, i n order to compare the change before and after the tests in two groups, one was used for repeated measures analysis of variance(repeated ANOVA) and the Wilcoxon Signed-Rank test. To compare the change before and after between two groups were analyzed by independent t-test and Mann-Whitney test. The level for statistical significance was set at p<.05 for all data. Results: There were significant difference on before and after experiment between VSGT and GGT on spatiotemporal element(p<.05). VSGT and GGT score were significantly incresed(p<.05) on before and after experiment. There were significantly decrese on TUG, 10mWT time for both VSGT and GGT(p<.05). Conclusion: Gait training in a variety of stimulating and balancing abilities of stroke patients is more effective than regular ground gait training, To improve the walking ability and the balance of stroke support instability through gait training stimulates a number of different obstacles over a surface, which provides an effective treatment

그레이스우수논문상(부인종양학)

신나리 ( Na Ri Shin ),( Yoo Young Lee ),( Seung Hyun Kim ),( Chel Hun Choi ),( Tae Joong Kim ),( Jeong Won Lee ),( Duk Soo Bae ),( Byoung Gie Kim ) 대한산부인과학회 2015 대한산부인과학회 학술대회 Vol.101 No.-

Objective: The purpose of this study is to investigate the prognostic role of pretreatment anemia in patients with early cervical cancer who underwent radical hysterectomy. Methods: In this study, we retrospectively enrolled patients with early cervical cancer (International Federation of Obstetrics and Gynecology stage 18 to ItA) who were treated at Samsung Medical Center, Seoul, Korea, from 1996 to 2007. Results: We retrospectively enrolled 805 patients. Median pretreatment hemoglobin (Hb) level was 12.8 g/dL (4.0-16.9) in all patients. Ninety-ninth out of 805 patients had pretreatment anemia (12.3%). Pretreatment anemia was significantly associated w th large tumor size, advanced clinical stage, and parametrial invasion. In multivariate analysis, higher pretreatment Hb entailed better prognostic significance in disease free survival (hazard ratio [HRl. 0.88; 95% confidence interval [GI}, 0.078-0.99) but not in overall survival (HR, 0.94; 95% Ct, 0.80-1.10). Conclusion: In conctusion, we found that the negative association between pretreatment Hb level and tumor size and the impact of anemia before treatment on disease free survival adjusted for other factors including clinica! stage and pathOlogical findings in early stage cervical cancer.