택사 (澤瀉, Alismatis Rhizoma) 추출물이 RBL-2H3 비만세포와 OVA/alum 감작 생쥐의 알레르기 염증 반응에 미치는 영향

송지현,이진화,김은진,김윤희,Song, Ji Hyun,Lee, Jin Hwa,Kim, Eun Jin,Kim, Yun Hee 대한한방소아과학회 2018 대한한방소아과학회지 Vol.32 No.3

Objectives Alismatis Rhizoma has been known to suppress inflammation and allergic reaction. However, the cellular target of Alismatis Rhizoma and its mechanism of action remain unclear. This study was designed to examine the effect of Alismatis Rhizoma extract (ALC) on the RBL-2H3 mast cells in vitro and on the OVA/alum sensitized mice ex vivo. Methods In the study, RBL-2H3 mast cells were cultured in minimal essential medium (MEM) for 24 hours, and treated separately with cyclosporin A and varying doses of ALC, and then stimulated with Phorbol 12-myristate 13-acetate (PMA) (50 ng/ml) and Ionomycin ($0.5{\mu}M$). The levels of IL-13, IL-4 were measured by ELISA analysis. The mRNA levels of IL-4, IL-5, IL-6, IL-13, GM-CSF, $TNF-{\alpha}$ were analyzed with Real-time PCR. Also, manifestations of MAPKs transcription factors and $NF-{\kappa}B$ p65 translocation were analyzed by western blotting in vitro. Subsequently, for ex vivo experiment, we induced allergic inflammation on Balb/c mice by OVA/alum and administered ALC orally. And we measured serum OVA-specific IgE level and IL-4, IL-13 in the splenocyte culture supernatant by ELISA analysis. Results ALC was shown to suppress mRNA expression of IL-4, IL-5, IL-6, IL-13, GM-CSF, $TNF-{\alpha}$, and to inhibit the IL-13, IL-4 production. Also ALC reduced an activation of mast cells specific signal MAPKs transcription factors and $NF-{\kappa}B$ p65 from the western blot analysis in in vitro experiment. In ex vivo, ALC oral adminstration decreased the level of OVA-specific IgE in serum, and IL-4, IL-13 in the splenocyte culture supernatant. Conclusions ALC is shown to reduce inflammation and allergic response by suppressing Th2 cytokines through the regulation of transcription factors MAPKs and $NF-{\kappa}B$ p65 in mast cells. Administration of ALC suppressed OVA-specific IgE in ovalbumin allergy model through the inhibition of Th2 cytokine. In conclusion, ALC can be considered as an effective treatment for allergic diseases such as atopic dermatitis.

삼복첩의 치료 효과와 만족도에 대한 연구

송지현,이진화,김윤희,Song, Ji Hyun,Lee, Jin Hwa,Kim, Yun Hee 대한한방소아과학회 2018 대한한방소아과학회지 Vol.32 No.1

Objectives The purpose of this study is to understand effect on respiratory disease and satisfaction of parents about acupoint sticking in hottest period of summer. Methods A survey was conducted to the parents of their children who took acupoint sticking in Dog-days for two years in a row. 17 children (10 boys, 7 girls, 4.0-12.11 years) were attached Socheongo to BL-13, BL-15, BL-17 for 3 hours. In terms of the effectiveness of the therapy, the treatment only considered as effective when there is any improvement in respiratory symptoms. Results 11 children (64.7%) from the study group have shown positive effect. There were significant improvements in frequency of having a cold or duration of a cold; duration of rhinitis; frequency of having empyema or its duration; frequency of having bronchitis; frequency of having pneumonia; frequency of having otitis media. Respiratory health VAS (Visual Analog Scale) had significant increase from 5 to 6.71 (p<0.05). 7 parents (41.2%) were satisfied with acupoint in hottest period of summer and 16 parents (94.1%) wanted to have their children get treated again in next year. From the survey, inconveniences of acupuncture were weak attachment of the acupoint (23.5%), long attaching time (11.8%), pressure about number of treatments (5.9%), worry about side effects (5.9%). Side effects have been reported; 2 pruritus (11.8%), 1 pruritus and erythema (5.9%). Conclusions Acupoint sticking in hottest period of summer improves lung symptoms in children older than three years old. However, the ways to reduce the side effects and inconveniences are needed.

발암원을 투여한 쥐에서 들기름이 대장 종양발생과 조직의 지방산 조성 및 Eicosanoids 생성에 미치는 영향

송지현,박현서 ( Ji Hyun Song,Hyun Suh Park ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.6

The study was to observe the effect of perilla oil rich in a-linolenic acid(18:3ω3) on the incidence of colorectal tumor and its relation to the levels of plasma TXB₂ and colonic mucosal PGE₂ and fatty acid profiles of colonic mucosal lipid and hepatic microsomal phospholipid in colon carcinogenesis. Male Sprague Dawley rats, at 7 weeks of age, were divided into 2 groups and intrarectally infused with saline or chemical carcinogen (methylnitrosourea, MNU) and fed 16% (w/w) fat diet containing one of dietary fats (beef tallow, com oil, perilla oil) for 30 weeks. Large number of tumors were found in the distal colon of MNU-treated rats by all fats and tumor incidence was lower in the rats fed perilla oil (57.7%) compared to those of com oil (82.1%) or beef tallow (87.5%) diet. The overall fatty acid profiles of colonic mucosal lipid and hepatic microsomal phospholipid reflected dietary fatty acids. The levels of C20:4 were similarly higher in tissues of beef tallow or com oil fed rats even though dietary levels of C18:2 were significantly different in two groups, which suggests that there might be some regulatory mechanism present to control the level of C20:4 in tissue. The decreased level of C20:4w6 and increased level of w3 fatty acids in perilla oil fed rats were associated well with the decreased levels of plasma TXB₂ and colonic mucosal PGE₂. Perilla oil rich in ω α-linolenic acid decreased the incidence of colon tumor possibly through modification of tissue fatty acid profile and eicosanoid synthesis.

자동차 제작결함조사 통계 분석을 통한 리콜제도 연구

송지현,권해붕,이광범,김희준,Song, Ji-hyun,Kwon, Hae-boung,Lee, Kwang-bum,Kim, Hee-june 한국자동차안전학회 2015 자동차안전학회지 Vol.7 No.4

The basic point of a vehicle recall is to remove vehicle defects as soon as possible and thus prevent possible road traffic accidents caused by the defects beforehand. Therefore, the core of vehicle recall under the self-certification system consists of a timely response and fast remedy of defects. The present study aimed to deduce a plan for improvement of the system necessary for the fast remedy of defects through a phased analysis of defect investigation procedure based on defect investigation statistical data. There will be a need to make the TSB(Technical Service Bulletin) or service campaign data submission of a manufacturer compulsory for the collection of broad defect information in the stage of information analysis and to impose a higher penalty when the manufacturer violates the data submission in the investigation stage. In addition, it is considered that an active service campaign should be induced and a punishment for late recall will be needed for consumer protection.

3GPP2 SMV의 실시간 음성/음악 분류 성능 향상을 위한 Gaussian Mixture Model의 적용

송지현,이계환,장준혁,Song, Ji-Hyun,Lee, Kye-Hwan,Chang, Joon-Hyuk 한국음향학회 2007 韓國音響學會誌 Vol.26 No.8

본 논문에서는 음성 인식과 음악 인식에서 뛰어난 성능을 보이는 Expectation-Maximization(EM) 알고리즘 기반의 패턴인식기법인 가우시안 혼합모델(Gaussian Mixture Model, GMM)을 이용하여 기존의 3GPP2 Selectable Mode Vocoder(SMV)의 실시간 음성/음악 분류 성능을 향상 시키는 방법을 제안한다 SMV의 음성/음악 실시간 분류 알고리즘에서 사용된 특징벡터와 분류방법을 분석하고, 이를 기반으로 분류성능향상을 위해 패턴인식 알고리즘인 GMM을 도입한다. 구체적으로, SMV의 음성/음악 분류알고리즘에서 사용되어진 특징벡터만을 선택적으로 사용하여 효과적인 GMM을 구성한 실시간 분류기법이 제시되었다. SMV의 음성/음악 분류에 적용한 GMM의 성능 평가를 위해 SMV 원래의 분류알고리즘과 비교하였으며, 다양한 음악장르에 대해 시스템의 성능을 평가한 결과 GMM을 이용하였을 때 기존의 SMV의 방법보다 우수한 음성/음악 분류 성능을 보였다. In this letter, we propose a novel approach to improve the performance of speech/music classification for the selectable mode vocoder(SMV) of 3GPP2 using the Gaussian mixture model(GMM) which is based on the expectation-maximization(EM) algorithm. We first present an effective analysis of the features and the classification method adopted in the conventional SMV. And then feature vectors which are applied to the GMM are selected from relevant Parameters of the SMV for the efficient speech/music classification. The performance of the proposed algorithm is evaluated under various conditions and yields better results compared with the conventional scheme of the SMV.

절수시 Mongolian gerbil (Meriones unguiculatus) 콩팥의 Aquaporin 2, Aquaporin 4 발현변화

송지현,권진석,김용환,박용덕,한기환,류시윤,정주영,Song, Ji-Hyun,Kwon, Jin-Seuk,Kim, Yong-Hwan,Park, Yong-Deok,Han, Ki-Hwan,Ryu, Si-Yun,Jung, Ju-Young 대한수의학회 2007 大韓獸醫學會誌 Vol.47 No.4

Mongolian gerbil (Meriones unguiculatus) has been as an model animal for studing the neurological disease such as stroke and epilepsy because of the congenital incompleteries in Willis circle, as well as the investigation of water metabolism because of the long time-survival in the condition of water-deprived desert condition, compared with other species animal. Aquaporin 2 (AQP2) expressed at the surface of principal cells in collecting duct results from an equilibrium between the AQP2 in intracellular vesicles and the AQP2 on the plasma membrane. Aquaporin 4 (AQP4), which is expressed in cell in a wide range of organ, is also present in the collecting duct principal cells where this is abundant in the basolateral plasma membranes and represent potential exit pathways from the cell for water entering via AQP2. In this research, we divide 3 groups of which each group include the 5 animals. In the study of 7 or 14 days water restricted condition, we investigated the AQP2 and AQP4 by using a quantitative immunohistochemistry in the kidney. The results obtained in this study were summarized as followings. AQP2 is abundant in the apical plasma membrane and apical vesicles in the collecting duct principal cell and at rare abundance in connecting tubules. In the water-deprived Mongolian gerbil kidney, expression of AQP2 was continuosly increased in the cortical collecting duct and inner medullary collecting duct. This increase was both the apical region and cytoplasm. AQP4 is mainly expressed in the inner medulla, although some expression is also noted in the more proximal segment. In the water-deprived Mongolian gerbil kidney, AQP4 was also increased in the inner medullary collecting duct. Immunoactivity was increased in entire inner medullary collecting duct and newly detected in cytoplasm of principal cell. These findings suggest that increased levels of AQP2 and AQP4 in the cortical and inner medulalry collecting duct may play a important role for maintain fluid balance in the water-deprived kidney.

다문화가족 부부의 결혼적응요인에 관한 연구

송지현 ( Song Ji-hyun ),이태영 ( Lee Tae-young ) 한국보건사회연구원 2010 保健社會硏究 Vol.30 No.2

본 논문은 대전·충청지역에 거주하고 있는 다문화가족 부부를 대상으로 그들의 결혼적응에 영향을 미치는 요인들을 파악하고 부부간에는 어떠한 차이를 보이는지 분석 하였다. 그 결과, 남편들의 경우 자녀의 수가 적을수록, 의사소통 효율성이 높을수록, 자문화 전달태도를 취할수록, 문화적응 스트레스가 낮을수록 결혼적응이 높게 나타났다. 반면에 아내들은 결혼기간이 짧을수록, 의사소통 효율성이 높을수록, 다문화 수용 태도와 자문화 전달태도를 취할수록, 문화적응 스트레스가 낮을수록 결혼적응이 높게 나타났다. 요약하면 부부간의 의사소통 효율성과 자문화 전달태도, 즉 문화적응이 결혼적응에 영향을 미치는 매우 중요한 요인임을 확인하였다. 본 논문은 성별에 따른 결혼생활의 차이를 이해하고, 부부사이의 친밀도를 더욱 높여 긍정적 관계를 유지할 수 있도록 도움을 주는데 의의가 있으며, 연구결과를 바탕으로 다문화가족 부부의 결혼적응을 높일 수 있는 다양한 가족복지실천방안에 대해서도 제언하였다. The purpose of this study was to examine factors affecting the marital adjustment of couples from multicultural families and any possible differences between the husbands and wives in that regard. It`s basically meant to get a grip on gender gap in marriage life to boost the intimacy and relationship of married couples. The subject in this study were couples from multicultural families in the regions of Daejeon and Chungcheong province, who included 130 husbands and 153 wives. A structured survey was conducted to gather data. The major findings of the study were as follows: concerning factors affecting marital adjustment, the husbands were better adjusted when they had fewer children, when they had a more efficient communication with their wives, when they made more efforts to convey Korean Culture to their wives, and when they suffered less stress due to cultural adjustment. In the event of the wives, a shorter length of marriage life, higher communication efficiency, better receptive attitude to multiculture, stronger attitude of conveying native culture to spouses and less cultural adjustment stress led to better marital adjustment. The above-mentioned findings suggested that conjugal communication efficiency and cultural adjustment have the most crucial impact on satisfaction level with marriage life.

골반내 염증 환자에서의 클라미디아 트라코마티스의 감염에 대한 임상적 고찰

송지현 ( Ji Hyun Song ),송성래 ( Seong Rae Song ),송재훈 ( Jae Hun Song ),정영욱 ( Young Wook Jung ),민정원 ( Jeong Won Min ),이석수 ( Suk Soo Lee ) 대한산부인과학회 2005 Obstetrics & Gynecology Science Vol.48 No.3

목적: 클라미디아 트라코마티스는 골반 내 염증을 일으키는 원인균 중 가장 흔한 것으로, 생식기 여성에서 불임, 난관 임신, 난관 폐쇄, 불임 등의 여러 합병증을 유발하고, 임산부에 있어서도 주산기 감염, 조기 진통, 신생아 결막염을 유발하는 등 임상적 중요성이 크다. 이에 클라미디아 감염의 유병률을 구하고, 임상적 특성을 분석하였다. 연구 방법: 2003년 3월부터 2004년 4월까지 산부인과를 방문하여 골반 내 염증으로 진단받은 입원 환자 137명과 Objective: Chlamydia trachomatis is one of the most common causative microorganism in pelvic inflammatory disease (PID). In this infection tubal obstruction, infertility, tubal pregnancy and recurrent pelvic infection has involved. Also, perinatal infecti

兒歌를 활용한 유아기 어린이 중국어 교수-학습 방법 제안

宋之賢(Song Ji-hyun) 중국어문학연구회 2010 중국어문학논집 Vol.0 No.65

韓ㆍ中 반의어 대조와 반의어의 교육적 활용

宋之賢(Song, Ji-hyun) 중국어문학연구회 2014 중국어문학논집 Vol.0 No.87

This article analyzes the structural, semantic, rhetorical, and social-economic differences in antonyms between Chinese and Korean and explores the reasons for errors in Korean students learning Chinese antonyms. This reveals inter-lingual, and inter-cultural commonalities and characteristics in the context of Chinese antonym instruction. The comparison between Chinese and Korean antonyms serves to raise the efficacy of Chinese language instruction and learning among Korean students. The results prove significant in cognitive linguistics and inter-cultural relations.