중만생 항산화 적색메벼 ‘건강홍미’

송유천(You-Chun Song),조준현(Jun-Hyeon Cho),이지윤(Ji-Yoon Lee),이종희(Jong-Hee Lee),오성환(Sung-Hwan Oh),한상익(Sang-Ik Han),정국현(Kuk-Hyun Jung),서우덕(Woo-Duck Seo),황운하(Woon-Ha Hwang),장기창(Ki-Chang Jang),박노봉(No-Bong Park) 한국육종학회 2017 한국육종학회지 Vol.49 No.1

To fit the market demands for functional rice such as black and red color rice, ‘Sugary’a high free sugar content line was backcrossed to the black rice ‘Milyang152. The pedigree method was adapted in selection and generation advance. A high polyphenol content line, YR19646-3-2-1-1-2-2 showing red pericarp was selected and designed as ‘Milyang234’ followed by variety name ‘Geonganghongmi’ in 2010. Heading date of ‘Geonganghongmi’ was Aug. 19, medium-late maturing cultivar. ‘Geonganghongmi’ showed resistance to leaf blast and rice stripe virus while susceptible to major diseases and insects. The total polyphenol content of ‘Geonganghongmi’ was high as much as 24.2mg/g compare to that of 4.9mg/g of ‘Nampyeongbyeo’ and 17.2mg/g of a red pericarp rice ‘Jeogjinju’ at 70% ethanol extraction conditions. In local adaptability tests, yield of ‘Geonganghongmi’ was 4.5MT/ha about 86% of ‘Nampyeongbyeo’ and 102% of ‘Jeogjinju’, respectively. ‘Geonganghongmi’ is suitable for southern and middle plain area of Korea.

중만생 기능성 당질미 ‘단미’

송유천(You-Chun Song),이종희(Jong-Hee Lee),이지윤(Ji-Yoon Lee),김춘송(Choon-Song Kim),김세리(Se-Ri Kim),정국현(Kuk-Hyun Jung),박노봉(No-Bong Park),곽도연(Do-Yeon Kwak),하운구(Woon-Goo Ha),이점식(Jeom-Sig Lee),강항원(Hang-Won Kang),조준 한국육종학회 2015 한국육종학회지 Vol.47 No.4

‘Danmi’, a sugary rice (Oryza sativa L.), was developed by the Department of Functional Crops, NICS, RDA in 2008. This variety was derived from a cross between ‘Sugary’ a genetic stock of germplasm as a source of sugary endosperm and ‘Seomjinbyeo’ in summer of 1994. The pedigree of ‘Danmi’ is YR16541-14-3-1-2-1-1-3-1-1 which was designated as ‘Milyang 227’ in 2005. Heading date of ‘Danmi’ is August 19, a medium-late maturing ecotype. The total sugar content of ‘Danmi’ was 21.7% with fructose (1.16%), glucose (7.87%), sucrose (9.69%), maltose (0.52%), and raffinose (2.13%), respectively. The appearance of grain is flat shape with 1,000 grain of brown rice is 16.1g. The yield potential of ‘Danmi’ in brown rice is 2.58MT/ha at ordinary fertilizer level of local adaptability test from 2006 to 2008. The sugary rice ‘Danmi’ would be adaptable to the plain paddy field of middle, honam and yeongnam province of Korea.

국내 육성된 향미 품종의이앙시기별 이화학적 특성 및 향기성분 비교 분석

조준현,송유천,이광식,최식원,이미자,장기창,김현영,강현중,박기도,서우덕,Cho, Jun Hyun,Song, You Chun,Lee, Kwang Sik,Choi, Sik Won,Lee, Mi Ja,Jang, Ki Chang,Kim, Hyun Young,Kang, Hyeon Jung,Park, Ki Do,Seo, Woo Duck 한국환경농학회 2017 한국환경농학회지 Vol.36 No.3

1. 본 연구의 향미의 품종별, 이앙시기별 이화학적 특성 평가한 결과 품종간의 차이는 나타나지 않았으며 단백질 함량은 5.8~7.3%로 나타났고 5월 22일 조기이앙시 평균 6.4%로 가장 낮았으며 지방 함량은 조기이앙시 평균 $2.6{\pm}0.1%$, 보통기이앙시 평균 $2.5{\pm}0.1%$, 만기이앙시 평균 $2.2{\pm}0.1%$를 나타내어 이앙시기가 빠를수록 높은 경향을 나타내었다. 2. 향미 품종 중 Hyangmibyeo 2 ho가 강하점도의 값이 152.3RVU로 가장 높아 전분의 구조가 호화되기 쉬운 구조적 특성을 가지고 다른 품종에 비해 밥맛이 우수 할 것으로 판단되며 이앙시기는 조기나 보통기 이앙이 좋을 것으로 판단된다. 3. 향미 품종, 이앙시기별 향기성분 정량 분석 비교에서는 향기성분 등 중 대표적으로 2-acetyl-1-pyrroline (2AP)를 지표성분으로 정하여 정량분석 한 결과 품종 중에는 Aromi가 $41.7{\sim}66.7{\mu}g/100g$으로 가장 높은 2AP의 함량을 나타내었고 이앙시기의 영향은 모든 품종에서 각각 보통기이앙시 평균 $37.4{\mu}g/100g$, 만기이앙시 $31.5{\mu}g/100g$ 조기이앙 시 평균 $37.4{\mu}g/100g$ 순으로 나타났다. 최적 이앙시기는 생산력 검정 등의 다양한 요소를 고려해 볼 때 6월 5~20일 사이에 이앙하는 것이 최적으로 판단된다. BACKGROUND:The Aromatic rice which is characterized by the flavor of Nurungji when cooked rice, and consumption is increasing recently. The purpose of this study was to investigate the physicochemical characteristics and aroma components of five aromatic rice cultivars according to transplanting time. METHODS AND RESULTS: Quantitative analysis of protein, fat, fatty acid and essential amino acid for five aroma rice cultivars(Hyangmibyeo 2 ho, Aromi, Mihyang, Aranghyangchal, Heughyang)and transplanting time was analyzed by crude protein analyzer, gas chromatography (GC), liquid chromatography (LC) and viscosity analysis was done by using rapid viscosity analyzer (RVA). The content of 2-acetyl-1-pyrroline (2AP) was determined by gas chromatography mass spectrometer. (GC-MS) As a result, the average protein and lipid contents were 6.5% and 2.4%, respectively. The content of essential amino acid showed the highest content at 104.4mg/g. There was no significant change in normal nutrients during the transplanting time. By RVA, cv.Hyangmibyeo 2 ho showed the highest peak and total setback viscosities and lowest breakdown viscosity in early transplantation. The content of 2AP in flavor varieties and transplanting time was quantitatively analyzed by GC-MS. Among the cultivars, Aromi showed the highest 2AP contents at $66.7{\mu}g/100gin$ normal transplanting time. CONCLUSION: cv.Aromi and Hyangmibyeo 2 ho were excellent physicochemical properties and 2AP components contents amongaromatic rice cultivars tested. Theiroptimaltime to transplant was at the beginning of June in the area of Miryang.

쌀의 물리적 특성과 식미와의 상관

김영두,하운구,송유천,조준현,양은인,이재길,Kim Young Doo,Ha Un Gu,Song You Chun,Cho Jun Hyeon,Yang Eun In,Lee Jae Kil 한국작물학회 2005 Korean journal of crop science Vol.50 No.suppl1

밥의 식미관련 특성에 영향을 미치는 요인을 알아보기 위해 쌀의 물리적인 특성과 식미치와의 상관을 비교하였다. 공시재료로 일품, 호안, 호진, 신동진 및 고시히까리를 사용하여, 식미치, 공극률, 팽창률, 가열시간, 색도, 수분흡수율, 경도, 찰기 둥을 분석한 결과는 다음과 같다. 1. 식미치가 높은 고시히까리의 경우 공극률 및 팽창률은 19.0 및 $286\%$로 낮은 값을 보인 반면, 찰기 및 가열시간에서는 높은 값을 보였고, 수분흡수율은 고 시히까리 및 신동진이 일품, 호진 및 호안보다 느렸다. 2. 팽창률(-0.3897), 공극률(-0.8736) 및 수분 흡수속도(-0.9601)는 식미치와 부의 상관을 보인반면, 가열시간(0.8608), Waxiness(0.9198)및 경도(0.4779)는 식미 치와 정의 상관성을 보였다. 3. 식미치에 대한 각 물리적 특성들의 기여도에서는 수분 흡수속도가 $35.9\%$로 기여도가 가장 크고, 다음으로 공극률$(22.7\%)$, 가열시간$(17\%)$ 및 찰기$(8.2\%)$ 순 이었다. Many parameters such as textural, cooking and pasting properties and chemical components aye the main reasons of tardy progress in evaluation of cooked rice. To increase the evaluation effect of cooked rice, physical properties and palatability of cooked rice were studied with five varieties. The highest palatability was observed in Kosihikari while Hoan showed the lowest value. The cooked rice of Kosihikari showed a high values of waxiness (0.12) and boiling time (21.1 min.) while the porosity and expansibility were as low as 19.0 and $28.6\%$, respectively. The water absorption time of Kosihikari and Sindongin was a little bit slow compare to the other varieties. A negative correlations were observed between palatability and expansibility (-0.390), porosity (-0.874**) and water absorption time (-0.960**) while a positive correlations were observed between palatability and boiling time (0.861**), waxiness (0.920**) and hardness (0.478). The path coefficient of water absorption time, porosity, boiling time and waxiness contributed to the palatability were 35.9, 22.7, 17.0 and $8.2\%$, respectively, Thus, the results obtained in this study suggested that the physical characteristics of vice grains could be effectively utilized in high quality of cooked rice breeding programs.

遺傳子的 雄性不稔과 葯培養을 利用한 벼의 標識遺傳子 集積

Hak Soo Suh(徐學洙),You Chun Song(宋裕千) 한국육종학회 1991 한국육종학회지 Vol.23 No.1

Fourteen Japonica marker lines were crossed to the Japonica genetic male sterile (GMS) rice Milyang 67ms, and thirteen marker lines of Tongil type were crossed to the Tongil type GMS rice Milyang 55ms. The Fls were backcrossed to the GMS parent respectively. From the fertile BC1F1 plants, BC1F2 seeds were bulk harvested separately from Japonica group and Tongil group. These two populations of BC1F2s were grown in isolated field to facilitate composite crosses. From the male sterile plants of the BC1F2, the composite crossed seeds were harvested. The composite crossed plants were grown under green house condition in winter season, and anther culture and chromosome doubling techique were applied to get the homozygous lines for marker genes. The dihaploid plants induced from the anther culture of the composite crossed plants showed the segregation ratio of 1:1 for male sterility. Five to six marker genes could be accumulated and fixed by using GMS facilitated composite crosses and anther culture technique. The GMS facilitated composite crosses and anther culture technique was proposed as a breeding method for accumulating useful genes in rice.

저항전분 기능성 고아밀로스 벼 ‘도담쌀 (밀양261호)’

조준현(Jun Hyeon Cho),송유천(You Chun Song),이종희(Jong Hee Lee),이지윤(Ji Yoon Lee),손영보(Young Bo Son),오성환(Seong Hwan Oh),한상익(Sang Ik Han),김춘송(Chun Song Kim),정국현(Kuk Hyun Chung),박동수(Dong Soo Park),이점식(Jeom sig Le 한국육종학회 2019 한국육종학회지 Vol.51 No.4

‘Dodamssal’ was developed as a functional rice by means of a cross between ‘Goamibyeo’, a high amylose cultivar for noodle rice, and ‘Goami2’, a mutant cultivar derived from ‘Ilpum’ treated with N-methyl N-nitrosourea (MNU), with a high amylose content. The bulk population was displayed from F2 to F4, followed by pedigree methods from F5, where line selection was performed based on amylose mutants with an opaque endosperm. Finally, ‘Milyang261’, which has a high amylose content and high resistant starch content, was selected and named as ‘Dodamssal’ in 2013. ‘Dodamssal’ is a mid-maturing ecotype with a heading date of August 10, susceptibility to both viruses and insects, and showed a slightly high viviparous germination rate of 23.4%. The yield capacity of ‘Dodamssal’ was 529 kg/10a over a 3-year regional yield test and would be suitable for cultivation in the middle and southern plain areas of Korea. The amylose content of ‘Dodamssal’ is 42.8%, with a grain appearance of opaque endosperms. Moreover, the starch granule of ‘Dodamssal’ was a round particle shape, unlike the polygonal shape of the ordinary grain. ‘Dodamssal’ is functional variety with resistant starch and dietary fiber contents of 13.6% and 5.3%, respectively(Registration No. 5637).

벼 유전자원의 수당립수 증진 유전자 유전형 분석

신동진,김태헌,이지윤,조준현,이종희,송유천,박동수,오명규,Shin, Dongjin,Kim, Tae-Heon,Lee, Ji-Yoon,Cho, Jun-Hyun,Lee, Jong-Hee,Song, You-Chun,Park, Dong-soo,Oh, Myeong-Kyu 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.4

벼 수량성 증진을 위하여 수당립수 증진 유전자로 보고된 5종의 유전자에 대한 분자표지를 검정하고 유전자원 479점에서 이들의 유전자에 대한 유전형을 검정하였다. 판독이 용이한 Gn1a및 DEP1, Apo1유전자의 In/del 분자표지를 각각 개발하였고 Ghd7과 Nal1 유전자에 대하여서는 기존 보고된 SNP 분자표지를 이용하여 편리성을 검정하였다. 이들 분자표지는 아가로즈젤에서 각각의 유전형 판독이 용이하기에 벼 수량성 향상을 위한 분자육종에 적용이 가능할 것으로 기대되었다. 유전자원 479점에서 수당립수 증진 유전자 5종의 유전형을 분석하였을 때 총 13개의 haplogype으로 분류되었다. 대부분의 Indica 품종과 Japonica 품종은 haploptype 1과 haplotype 13에 속하였다. 나머지 haplotype에 속한 55점의 유전자원은 수당립수 증진 유전자에 대한 유전다양성을 보유한 자원으로 유전체 분석 등을 위한 핵심집단으로 활용할 수 있을 것으로 판단되었다. 유전자원 396점의 수량구성요소를 비교하였을 때, Nal1을 제외한 4종의 수당립수 증진 유전자의 수량증진 대립유전자형에서 이삭 수가 0.6 ~ 0.8개/주 감소하였으나 수당립수는 이삭당 27 ~ 29개 증진되었다. Nal1 유전자는 유전적 배경에 따라 효과가 다르게 나타나며, Nal1-japonica 대립유전자형의 수당립수 증진 효과보다 Nal1-indica 대립유전자형이 감소효과가 큰 것으로 추측되었다. 앞으로 본 논문에서 검정된 수당립수 증진 분자표지 5종과 유전자원의 유전형을 정보를 바탕으로 벼 수량성 증진 육종에 활용하고자 한다. ARice is an important staple food in the world and rice yield is one of the main traits for rice breeding. Several genes involved in increasing the yield have been identified through map-based gene cloning within natural variations in rice. These identified genes are good targets for introducing a genetic trait in molecular breeding. Here, we chose five genes reported to be involved in increasing grain number per panicle in rice; Gn1a, dep1, Apo1, Ghd7, and Nal1. We developed In/Del markers for Gn1a, and dep1, Apo1, and applied the reported SNP markers for Ghd7 and Nal1. We were easily able to examine the genotype of each gene on agarose gel. We tested the genotypes on 479 rice resources that we held with evaluated molecular markers. According to the genotype of each gene, rice resources were divided into 13 haplotypes, and most of the Indica and Japonica varieties were included in haplotypes 1 and 13, respectively. When we examined the effect of each gene on grain number per panicle and panicle number per plant, panicle number per plant in the yield negative allele group for each gene was reduced by approximately 0.3 to 0.8 compared to that in the yield positive allele group. However, the number of yield positive alleles for each gene was higher by about 21 to 27 grains per panicle than that of yield negative alleles. Although most of the varieties were grouped in haplotypes 1 and 13, we believe that this genotype information with evaluated molecular markers will be useful in rice breeding for increasing the yield with grain number per panicle.

완전미율이 우수한 조생종 벼 중간모본 “중모1019호”

이종희(Jong-Hee Lee),여운상(Un-Sang Yeo),김춘송(Choon-Song Kim),이지윤(Ji-Yoon Lee),송유천(You-Chun Song),박노봉(No-Bong Park),오성환(Seong-Hwan Oh),김상열(Sang-Yeol Kim),정국현(Kuk-Hyun Jung),김우재(Woo-Jae Kim),손영보(Young-Bo Sohn 한국육종학회 2016 한국육종학회지 Vol.48 No.3

Jungmo1019 early maturing rice variety was developed by the rice breeding team of the Department of Southern Crop, NICS, RDA in 2011. This cultivar was derived from the cross Hwayeongbyeo//Hitomebore/Nampyeongbyeo made in 2005 and the production of doubled haploid rice plant from anther culture in F1 generation. Testing in the replicated yield trial in 2008, a promising line YR25312Acp62 was selected and it was designated as the Milyang246. The early traus plauting local adaptability test of Milyang246 was carried out at three location in 2011 and it was named as Jungmo1019 showing a high head rice ratio and early -maturity eco-type. Number of spikelet/panicle and 1,000 grain weight of brown rice was slightly lower than those of Jopyeongbyeo. This variety is resistant to bacterial blight, rice stripe virus disease but susceptible to major insect and pests. The milled rice yield of Jungmo1019 is 4.52MT per ha at the early transplanting of the local adaptability test. This line should serve as a useful source for improving head rice ratio at early transplanting cultivation in southern plain area.

동할미 발생이 적은 고품질 중만생종 벼 ‘영진’

이종희(Jong-Hee Lee),조준현(Jun-Hyeon Cho),이지윤(Ji-Yoon Lee),송유천(You-Chun Song),박동수(Dong-Soo Park),신동진(Dongjin Shin),이소명(So-Myeong Lee) 한국육종학회 2021 한국육종학회지 Vol.53 No.1

The rice variety ‘Youngjin’ is a japonica rice (Oryza sativa L.) with good eating quality, lodging tolerance, and resistance to therice stripe virus (RSV) and bacterial blight disease (BB). It was developed by the rice breeding team of the Department of Southern Crop,NICS, RDA, in 2016. This variety was derived from a three-way cross between ‘Iksan486’, ‘YR22722-B-B-B-25-1’, and ‘Chengmu,’ withgood eating quality, in the summer of 2007. The pedigree of ‘Youngjin’ was YR27874Acp46-1, and was designated as ‘Miyang283’ in 2012. After local adaptability tests at five different locations from 2014 to 2016, it was released under the name ‘Youngjin’ in 2016. ‘Youngjin’is a mid-late maturing ecotype with a short culm length of 67 cm. This variety was found to be resistant to the K1, K2, and K3 races ofBB, and RSV, and moderately resistant to leaf blast disease. The milled rice kernels of ‘Youngjin’ were translucent and clear, without whitecore and belly, and had good eating quality, according to a panel test. This variety showed a lower percentage of cracked kernels comparedto other susceptible cultivars. The milled rice yield of ‘Youngjin’ was 568 kg/10a, under normal levels of fertilization, determined by thelocal adaptability tests. This cultivar is adaptable to the Yeongnam plain area (Registration No. 7653).

系統育種, 集團育種 및 單粒系統育種法에 의해 育成된 벼 系統의 收量性 比較

Hak Soo Suh(徐學洙),Im Soo Choi(崔林守),You Chun Song(宋裕千) 한국육종학회 1991 한국육종학회지 Vol.23 No.2

The study was carried out to compare the pedigree, bulk and single seed descent(SSD) methods in rice breeding. Three selection methods were applied from F₂ to F₅ generations of the cross between line 3377 and Gayabyeo. Yield and yield components of the F₆ lines bred by the three methods were compared. No differences in numbers of panicles per hill, numbers of florets per panicle and 1,000 grain weight of the lines from pedigree and SSD methods were found, however, the three components were lower in bulk method than in pedigree and SSD methods. There was no difference in percent of filled grains in the three methods. Grain yields from pedigree and SSD methods were higher than that from bulk method, and no difference was found in the lines from pedigree and SSD methods.