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송노을,이준영,이윤열,박종대,장혜원 한국응용생명화학회 2019 Applied Biological Chemistry (Appl Biol Chem) Vol.62 No.-
A new solid phase microextraction (SPME)-Arrow method was evaluated for the analysis of volatile compounds in kanari-aekjeot, a Korean traditional salt–fermented sand lance sauce, and compared it to the standard headspace– SPME method. Factors observed to affect the extraction, including the fiber used, extraction temperature, extraction time, and NaCl concentration were carefully optimized. The Carboxen/Polydimethylsiloxane fiber exhibited the highest extraction efficiency for both analytical methods and was selected for further optimization of the extraction. The major volatile compounds extracted using both methods were 3-methyl butanoic acid, butanoic acid, acetic acid, 2,6-dimethylpyrazine, and benzaldehyde. The relative concentration (mg/L) of 3-methyl butanoic acid was 1.4-fold higher when using SPME. However, the SPME-Arrow method was more effective at extracting aromatic compounds including alcohol, aldehydes, and pyrazine. In particular, 3-methyl-1-butanol, 2-furanmethanol, and phenylethyl alcohol could only be detected using SPME-Arrow due to its larger sorbent volume. Thus, SPME-Arrow was evaluated as being more suitable for the extraction of pyrazines in sand lance fish sauce and might be useful for determining a broader range of volatile compounds in complex fermented foods.
송노을,백상호,송영란,권희정,임윤묵 한국고분자학회 2012 Macromolecular Research Vol.20 No.11
Oxygen generating (OG) hydrogels immobilizing glucose oxidase (GOx, EC 1.1.3.4) and peroxidase (PO) (EC 1.11.1.7) were prepared using a γ-ray irradiation polymerization technique, and the properties were examined. In physiological tests of the OG hydrogels ranging from 15 to 70 kGy, GOx or PO showed higher gel contents at 87% for the poly(vinyl alcohol) (PVA) hydrogel and 90% for the poly(vinylpyrrolidone) (PVP) hydrogel. The optimal irradiation conditions affecting the entrapment ratio for preparing the OG hydrogel was 35 kGy for the PVP hydrogel, and both enzymes (GOx, 95% and PO, 89%). Optimal GOx and PO activity of the prepared OG hydrogel occurred at pH 6.0 and 35 oC, and both enzymes showed stable activity under acidic conditions. The optimal glucose condition was 4 mg, which resulted in a maximum oxygen generating yield by the OG hydrogels. When oxygen concentration was compared, the OG hydrogels showed higher oxygen pressure than that of control hydrogels without the GOx and PO enzymes. Moreover, the OG hydrogel continuously converted potassium iodide to iodine in a glucose dosage and time dependant manner for 15 h, producing approximately 0.1 M iodine in 5 h.
당침지 검정콩 스낵 제품의 개발 및 기능성 성분의 변화
송노을(Nho-Eul Song),송영란(Young-Ran Song),김종희(Jong-Hui Kim),김영은(Young-Eun Kim),한아름(Ah-Reum Han),정도연(Do-Youn Jeong),백상호(Sang-Ho Baik) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.6
검정콩의 기능적, 영양적으로 매우 유용한 성분을 손쉽게 섭취할 수 있는 편리성을 갖춘 형태인 스낵 타입의 검정콩 제품을 개발하였다. 부드러운 조직감과 높은 기호도를 위해서 1차 당침지 조건/2차 당침지 조건을 달리하였으며 수분함량, 조직감, 관능평가 등을 고려하여 1차 당침지 당액농도 40°brix, 2차 당침지 당액농도 50°brix로 침지하여 제조한 당침지 콩스낵 제조공정을 확립하였고 그에 따른 최적 조건하에 제조된 당침지 검정콩 편이식 시제품에 대한 일반성분 분석을 실시한 결과 수분함량은 5.50±0.39%, 조단백질은 16.25±0.07%, 조지방은 34.26±3.95%, 조회분은 0.51±0.05%, 탄수화물은 43.48±0.01%이었으며 최종제품의 잔존당 함량은 10.20±0.10°brix로 나타났다. GABA 함량은 15.24±1.47 ㎎/g으로 9배 정도 증가되었으며, 이소플라본은 당이 분리된 alycon 형태로써의 daidzein(695.7±6.08 ㎍/g), genistein(810.6±3.9 ㎍/g) 함량보다 배당체 형태인 daidzin(755.8±39.6 ㎍/g)과 genistin(1640.7±390.8 ㎍/g) 형태로 많이 함유되어 있으며 페놀성분과 플라보노이드 함량은 각각 0.16±0.01 ㎎/g과 2.33±0.40 ㎎/g이 함유되어 있는 것으로 분석되었다. We developed and investigated sugar-soaked black soybean snack (SSBSS) to increase the availability of black soybean. The most desirable moisture contents and texture properties of hardness and gumminess in preparing SSBSS were obtained at a sugar concentration of 40°brix for the first sugar soaking step and 50°brix for the second sugar soaking process. Sensory evaluation also showed that SSBSS prepared with 40°brix for first sugar soaking step and 50°brix for the second sugar soaking process was graded higher than the other samples in all questionnaires. Prepared SSBSS had a final moisture content of 5.50±0.39%, crude protein content of 16.25±0.07%, and crude lipid content of 34.26±3.95%. The ash and carbohydrate contents of SSBSS were 0.51±0.05% and 43.48±0.01%, respectively, and the final glucose content was 10.20±0.10°brix. GABA concentration was 15.24±1.47 ㎎/g and the contents of daidzein, genistein, daidzin, and genistin were 695.7±6.08 ㎍/g, 810.6±3.9 ㎍/g, 755.8±39.6 ㎍/g, and 1640.7±390.8 ㎍/g, respectively. In addition, the phenolic compound and flavonoid contents in SSBSS were 0.16±0.01 and 2.33±0.40 ㎎/g, respectively.
Evaluation of various clean-up sorbents in kale followed by LC-MS/MS analysis of pesticides
정영성,송노을,최지연,황선혜,구민선,남태규 한국식품과학회 2022 Food Science and Biotechnology Vol.31 No.7
Chlorophyll-rich samples, such as kale, interfere with the analysis of residual pesticides and adversely affect the integrity of tandem mass spectrometers. Dispersed solid-phase (d-SPE) extraction using graphitized carbon black effectively removes pigments from kale extracts; however, it also reduces the recoveries of 30 pesticides. To overcome this, alternative sorbents, including ENVI-Carb, ChloroFiltr, and Z-Sep+, were evaluated in this study. A sorbent combination based on 50 mg of Z-Sep+ was most advantageous (21/30), good precision (< 15%), excellent pigment removal capacity, and low matrix effect. The limit of quantification (0.0001–0.0040 mg/kg) was lower than the Korean maximum residue limits levels. The proposed method was validated according to international guidelines and applied to real kale samples. The results demonstrated that d-SPE using Z-Sep+ provides an effective strategy for ensuring mass spectrometry system integrity and improving the analytical accuracy in chlorophyll-rich samples. This is a previ
송영란,김영은,김종휘,송노을,정도연,백상호 한국식품과학회 2011 Food Science and Biotechnology Vol.20 No.6
Fermented sugar-soaked black soybean snacks (FSBSS) were prepared using optimized conditions at 2times sugar soaking process to give a desirable form and texture after fermentation with Lactobacillus plantarum (LP) and Bacillus subtilis (BS) for 48 h. The prepared FSBSS showed higher total polyphenol and flavonoid contents than an unfermented (NF) sample; total isoflavone content increased approximately 31 and 17% (LP and BS,respectively), as compared to NF. γ-Aminobutyric acid was also contributed by LP and BS, indicating significantly higher contents of 16.9 and 21.6 mg/g (LP and BS,respectively) compared to NF (15.2 mg/g). Moreover, the FSBSS showed antioxidant activities on DPPH, superoxide,and hydroxyl radicals as compared to NF. Based on the results, the newly developed FSBSS using solid-fermentation indicate a useful and convenient way to replace traditional soybean products from the standpoint that also provides an easy means to supply high nutritive and functional properties.
Aptamer-based Fluorescent Assay for Simple and Sensitive Detection of Fipronil in Liquid Eggs
Tai-Yong Kim,Ji Won Lim,임민철,송노을,우민아 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.2
In the present study, an aptamer-based fluorescent assay was developed to detect fipronil in liquid eggs, using a carboxyfluorescein (FAM)-labeled aptamer (FAM aptamer) and partially complementary DNA labeled with carboxytetramethylrhodamine (TAMRA cDNA). The fipronil amount in analytical samples was determined by measuring FAM fluorescence at 522 nm. In the absence of fipronil, FAM fluorescence is effectively quenched by fluorescence resonance energy transfer (FRET) phenomenon generated by the hybridized structure of the FAM aptamer and TAMRA cDNA. In contrast, in the presence of fipronil, FAM fluorescence is gradually recovered in a fipronil concentration-dependent manner because the FAM aptamer has a stronger affinity for fipronil than for TAMRA cDNA. The proposed analytical method for fipronil involves a simple one-step procedure of mixing the sample solution without any washing step, and the results can be obtained in approximately 30 min. Under optimized conditions, the developed assay showed a linear response to fipronil in the egg sample in the range of 25–300 ppb with a detection limit of 53.8 ppb (0.068 mg/kg) and recovery rates in the range of 94.7-114.4%. Additionally, the assay selectivity for fipronil was investigated against eight pesticides, and it showed good specificity for fipronil. Compared with conventional liquid chromatography-mass spectrometry (LC-MS), the proposed method provides a simple, easy, and fast method to detect fipronil in egg samples without requiring bulky and expensive instruments and complicated and laborious processes. We believe this detection method could be a promising platform for fipronil detection in real food samples.