소 브루셀라병 표준시험관응집반응법 진단효율 평가

성소라 ( So Ra Sung ),김지연 ( Ji Yeon Kim ),허문 ( Moon Her ),이기찬 ( Ki Chan Lee ),구정희 ( Jeong Hui Gu ),강성일 ( Sung Il Kang ),이향근 ( Hyang Keun Lee ),김숙미 ( Suk Mi Kim ),정석찬 ( Suk Chan Jung ) 한국동물위생학회 2012 韓國家畜衛生學會誌 Vol.35 No.4

A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diag-nostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional vet-erinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked im-munosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three pre-scribed tests ranged high 84.1∼97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

국내 가축에서 브루셀라병에 대한 혈청학적 모니터링

성소라,김지연,허문,이기찬,강성일,이향근,조효림,이진주,정석찬,Sung, So-Ra,Kim, Ji-Yeon,Her, Moon,Lee, Kichan,Kang, Sung-Il,Lee, Hyang-Keun,Cho, Hyo Rim,Lee, Jin Ju,Jung, Suk Chan 대한수의학회 2014 大韓獸醫學會誌 Vol.54 No.4

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

20대가 인지하는 디자인에서의 고급감성에 관한 연구

성소라 ( Sung Sora ),나건 ( Nah Ken ) 한국산업디자이너협회(한국인더스트리얼디자인학회) 2016 산업디자인학연구 Vol.10 No.4

본 연구는 시대적 변화에 따른 고급의 의미 변화를 이해하고, 연구 대상자로 삼은 20대가 인지하는 고급감성 가치요인을 도출하는데 연구의 목적이 있다. 사회가 다변화 되면서 감성가치에 대한 관심이 지속적으로 증가함에 따라 감성 품격의 고급화가 디자인의 차별화 전략의 하나로 대두되고 있다. 고급감성에 관한 연구가 다른 분야에서는 지속적으로 진행되고 있으나, 현재 디자인분야에서는 미흡한 실정이다. 이러한 흐름 속에 디자인에서의 고급감성에 관한 연구는 의미 있다고 볼 수 있다. 본 연구의 주요 내용은 다음과 같다. 첫째, 문헌연구를 통해 고급의 의미변화에 대해 알아보고 디자인에서의 고급감성에 대한 의미를 분석하였다. 둘째, 연구의 대상자로 선정한 20대의 특징을 정리하고 분석하였다. 셋째, 20대 남·여 30명을 대상으로 1:1심층인터뷰를 실시한 후디자인 전문가 워크숍을 통해 감성언어를 분석하고, 20대가인지하는 디자인에서의 고급감성 가치 요소를 도출하였다. 넷째, 도출된 3가지 가치 요인별 디자인적 적용 가능성을 모색하기 위해 사례연구를 진행하고, 20대에게 고급감성을 어필하는 디자인 요소에 대해 정리하였다. 연구 결과, 20대가 인식하는 디자인에서의 고급감에 영향을 미치는 가치 요인은 크게 3가지(기능적 혁신성, 합리적 효율성, 정서적 공유성)로 나타났다. 또한 20대가 인식하는 디자인에서의 고급감성은 제품과 서비스를 사용할 때 인식되어지는 가치로, 사용자 개개인에게 자신에게 부여하는 경험 가치로 정의되었다. 본 연구는 사용자에 대한 이해를 바탕으로 디자인에서의 고급감성 요인을 도출해낸 연구로 20대가 인식하는 고급에 대한 새로운 정의를 발견하였다는 데 연구의 의의가 있다. 향후 연구에서 다른 세대에 동일한 방법론을 가지고 연구를 진행한다면, 세대별로 인식되는 디자인에서의 고급감성 가치요인에 대한 연구를 보다 심도 깊게 발전시킬 수 있을 것으로 기대한다. The objective of this study is to understand changes in the meaning of advancement from chronological changes and to derive value factors of high-quality perceived by people in their 20s as subjects in the research. Due to diversification in society, there has been an increasing concern on emotional value. Hereupon, advanced quality of sensitivity has become one of the strategies for differentiating the design. Studies have been continuously conducted in dealing with advanced sensitivity in other fields. However, it has not been much researched in design. Seen in this context, it is meaningful to conduct in dealing with high-quality in design. Major contents of this study are as follows. First of all, changes in meaning of advancement have been identified through literature review while analyzing the meaning of perceived high-quality in design. Secondly, characteristics of 20s selected as subjects in this study have been analyzed. Third, 1:1 in-depth interview has been conducted on 30 men and women in their 20s followed by analysis in emotional language through workshop with design experts. value elements perceived high-quality have been derived in design. Fourth, case study has been conducted to seek for the possibility of design-oriented application in three pre-derived value factors while organizing the characteristics of design. According to the result of study, there were three value factors(functional innovation, rational efficiency, and emotional sharing)that influenced on advancement in the design perceived by those in their 20s. In addition, high-quality perceived by the 20s in the area of design was the value recognized when using products and services and hence defined as empirical value granted to each of the users. This study is meaningful to find a new definition on advancement perceived by people in their 20s as a research deriving advanced sensitivity factors in the area of design based on an understanding on users. If proceeding a research with the same methodology on other generations in the follow-up study, it is expected to more deeply develop the research about advanced sensitivity value factors in the area of design recognized by each generation.

디자인에서 인식되는 고급감성의 표현에 관한 연구 ; 20대를 대상으로 한 CMF워크숍을 중심으로

성소라 ( Sora Sung ),나건 ( Ken Nah ) 한국산업디자이너협회(한국인더스트리얼디자인학회) 2018 산업디자인학연구 Vol.12 No.3

본 연구는 트렌드를 만들고 문화를 이끄는 20대를 대상으로 그들이 인식하는 디자인에서의 고급감성을 어떻게 표현하고 있는지 알아보기 위해 진행되었다. 이를 위해 첫째, 다양한 문헌 연구를 바탕으로 시대변화에 따른 디자인에서의 고급감성의 의미 변화를 이해하고, 인간의 감성을 만족시키는 감성디자인과 고급감성을 극대화 시키는 디자인 요소인 CMF의 중요성에 대해 알아보았다. 둘째, CMF워크숍을 통해 고급감성 어휘와 그에 따른 고급감성 소재 샘플을 추출하여, 감성 표현 방법에 대해 디자이너와 그 차이를 비교 분석하였다. 연구결과 20대와 디자이너가 인식하는 디자인에서의 고급감성에 대한 표현 방법은 차이가 있는 것으로 나타났다. 20대의 경우, 비유적 표현을 사용하여 자신이 느낀 느낌(feeling)을 표현하고, 자신의 경험에 따라 나타나는 감정(sensitive)과 기분(mood)을 다양한 어휘를 통해 표현하는 것을 알 수 있었다. 디자이너의 경우에는 소재의 형태나 컬러, 피니싱 등 디자인 요소를 직접적으로 표현하거나, 소재를 활용할 수 있는 구체적인 상황을 어휘로 표현하였다. 본 연구는 디자이너가 사용자가 느끼는 감성에 대한 중요성을 인식하고, 감성 조사 분석을 통해 사용자 경험을 만족시키는 CMF디자인 전략을 수립하는 토대를 마련하는데 그 의의가 있다. 향후 연구에서 심도 깊은 해석적 연구방법과 정량적 연구방법을 병행한다면 보다 더 객관화 된 연구결과를 도출 할 수 있을 것이라 기대한다. This study was conducted to find out how people in their 20s who create trends and lead culture express the high-quality sensibility in the design that they perceived. To do this, we learned about the meaning change of high-quality sensibility in design according to the change of time based on various literature studies, and the importance of CMF, which is a design element maximizing emotional design and high-quality sensibility that satisfies human sensibility. Second, we compared and analyzed the difference between people in their 20s and designers about the expressing high-quality emotions by extracting emotional vocabulary and material samples through CMF workshops. The results of the study showed that there is a difference in the way of expressing high-quality in the design perceived by in their 20s and designers. In case of people in their 20s, they were able to use figurative expressions to express their feeling and know how to express their emotions and mood that appear according to their experiences through various vocabulary. In case of designers, they expressed directly the design elements such as shape, color, and finishing of the material or expressed the specific situation in which the material can be utilized with technical term. This study is meaningful for designers to recognize the importance of the emotions felt by users and to lay the groundwork for establishing a CMF design strategy satisfying user experience through emotional research analysis. We expect that more objective research results would be produced derive in the future, if in-depth analytic and quantitative research methods would be combined in the future research.

디자인에서의 고급 감성 표현에 관한 연구 : 중국의 밀레니얼 세대와 부모세대의 감성언어를 중심으로

성소라(Sung, So RA),나건(Nah, Ken) 한국디자인리서치학회 2019 한국디자인리서치 Vol.4 No.1

본 연구는 비약적 경제성장을 통해 전세계가 주목하는 중국의 밀레니얼 세대와 그 부모세대의 감성언어를 중심으로 그들이 디자인에서의 고급 감성을 어떻게 표현하고 있는지 알아보기 위한 목적으로 진행되었다. 첫째, 문헌 고찰을 통해 세대 개념을 이해하고, 중국의 밀레니얼 세대와 부모세대의 특징을 정리하였다. 둘째, 시대 변화에 따른 고급의 의미 변화에 대한 이해를 바탕으로 중국이 인식하는 고급에 대해 시대 문화적 배경을 바탕으로 정리하였다. 셋째, 앞선 문헌 연구를 바탕으로 북경에 거주하는 밀레니얼 세대와 부모세대를 대상으로 사용자 연구를 진행하고, 각 세대별 인식하는 디자인에서의 고급감성에 대해 분석하였다. 그 결과 밀레니얼 세대와 부모세대가 인식하는 고급감성은 사회문화적 배경에 따른 개인의 경험의 폭에 따라 상이한 것으로 나타났다. 감성은 개개인 마다 다르게 느끼는 것으로 객관화 된 결과 도출을 위해서는 더 많은 대상자를 대상으로 상황과 맥락을 고려한 후속 연구가 필요하다. This study focuses on the emotional language of the Millennials and their parents in China whose remarkable economic growth captures the attention of the entire world, aiming to see how they express their high emotional quality in their design. First, the study grasps the concept of generation and summarizes the characteristics of the Millennials and their parents in China through literature review. Second, based on its examination of the meaning of high emotional quality which changes with the times, the study has described the Chinese perception of high emotional quality based on the cultural background of the times. Third, based on previous literature studies, the study researches users, mainly the Millennials and their parents who live in Beijing, analyzed the high emotional qual in design as perceived by different generations. As a result, it has turned out that the high emotional quality as perceived by the Millennials and their parents differ with the width of personal experience as influenced by the sociocultural background. Sensibility is differently perceived by different people. So, getting at objective results requires follow-up studies which deal with more subjects and conduct research that focus on situation and context.

모빌리티 패러다임 변화에 따른 사용자 경험디자인에 관한 연구

성소라(So Ra Sung) 한국디자인리서치학회 2023 한국디자인리서치 Vol.8 No.1

본 연구는 4차 산업혁명으로 인한 기술발전에 따라 변화된 모빌리티 환경에서 주 소비세대로 부상하고 있는 밀레니얼 세대가 정의하는 모빌리티의 의미를 고찰하고, 그들의 라이프스타일을 반영한 모빌리티 경험 시나리오를 바탕으로 미래 모빌리티 디자인의 전략 방향성을 제시하는데 연구의 목적이 있다. 이를 위해 첫째, 시대변화에 따른 모빌리티 개념 변화를 이해하고, 국내외 관련 제품 및 서비스를 분석하여 미래 모빌리티 환경을 예측하였다. 둘째, 변화의 주체인 밀레니얼 세대의 개념과 연구대상인 한국 밀레니얼 세대의 라이프스타일 특성을 문헌조사를 통해 이론적으로 정리하였다. 셋째, 한국밀레니얼 세대 50명을 대상으로 한 사용자조사를 통해 이들이 정의하는 이동의 의미를 분석하여 모빌리티 경험요소 6가지를 도출하였다. 넷째, 사용자 분석 결과를 바탕으로 한 전문가 워크숍을 통해 라이프스타일에 따른 3가지 유형에 따른 퍼소나 모델링을 진행하고, 모빌리티 경험 시나리오를 작성하였다. 연구의 결과가 추후 밀레니얼 세대를 대상으로 한 모빌리티 제품 및 서비스 개발을 위한 기초자료로 활용되길 기대한다. The purpose of this study is to examine the meaning of mobility defined by the millennial generation, which is emerging as the leading consumer in a mobility environment that has changed due to technological advances caused by the Fourth Industrial Revolution, and to present the strategic direction of future mobility design based on mobility experience scenarios that reflect their lifestyle. First, this study predicted the future mobility environment by understanding the changes in the concept of mobility according to the changes of the times and analyzing related products and services at home and abroad. Secondly, this study theoretically summarized the idea of the millennial generation, who are the drivers of change, and the lifestyle characteristics of the Korean millennial generation, which is the subject of this study, through a literature survey. Thirdly, through a user survey of 50 Korean millennials, this study derived 6 mobility experience elements by analyzing the meaning of movement defined by them. Fourthly, this study conducted persona modeling according to 3 types by lifestyle through expert workshops based on user analysis results, and prepared a mobility experience scenario. The results of this study are expected to be used as basic data for the development of mobility products and services for millennials in the future.

국내 서식 한우에서 큐열 항체 양성율 조사

김지연,성소라,편지인,허문,강성일,이향근,정석찬,Kim, Ji-Yeon,Sung, So-Ra,Pyun, Ji-In,Her, Moon,Kang, Sung-Il,Lee, Hyang-Keun,Jung, Suk Chan 대한수의학회 2014 大韓獸醫學會誌 Vol.54 No.3

Q-fever is a vector-borne (Coxiella [C.] burnetii) zoonotic disease that is an increasing public health concern. To date, some research about Q-fever prevalence in dairy herds and human patients has been reported in Korea, but information about Korean native cattle is scarce. To measure the prevalence rates of C. burnetii in Korean native cattle, a total of 1,095 bovine serum samples collected during 2010~2013 were analyzed with an enzyme-linked immunosorbent assay. Sixty-eight heads of cattle were diagnosed as positive and while 19 heads were suspected (positive rate = 6.2%). Interestingly, Jeju province had a seropositivity rate six times greater than that of other provinces (18.9% vs. 3.2%). High seroprevalence might be caused by wide distribution of ticks in Jeju province compared to other regions. Based on these data, extensive monitoring of C. burnetii infection in cattle, tick distribution, and climate changes is required.

토양 Metagenome Library로부터 고추역병 저해 클론 탐색

박해철,성소라,김동관,구본성,정병문,김진흥,윤문영,Park, Hae-Chul,Sung, So-Ra,Kim, Dong-Gwan,Koo, Bon-Sung,Jeong, Byeong-Moon,Kim, Jin-Heung,Yoon, Moon-Young 한국미생물학회 2009 미생물학회지 Vol.45 No.2

고추 역병을 야기하는 Phytophthora capsici 는 짧은 시간 내에 많은 면적에 피해를 주는 병으로 한번 발생하면 방제가 어려운 병으로 알려져 있다. 이러한 역병 곰팡이의 방제를 위하여 본 연구에서는 P. capsici의 염색체 복제 및 세포 골격 유지 등에 관여하는 단백질인 microtubule의 형성 저해를 유도하는 물질을 탐색하여 궁극적으로 고추역병 방제를 위한 연구를 진행하였다. 먼저 P. capsici alpha 및 beta tubulin을 E. coli BL21(DE3)에서 발현시켜 분리 정제하여 in vitro microtubule 형성을 확인하였다. P. capsici microtubule 형성 저해 metagenome clone 스크리닝을 위하여 경기도 수원의 여기산 토양에서 metagenome을 분리하여 library를 제작하여 Fluorescence Resonance Energy Transfer (FRET) 방법을 이용하여 P. capsici microtubule 형성을 저해하는 화합물을 탐색하였다. In vitro 스크리닝에서 약 384개의 metagenome library에서 2종의 clone을 선택하여 고추작물에 직접 방제하여 역병균의 생장 억제를 확인하였다. 이는 차후 고추역병 방제제 개발에 있어 중요한 후보물질뿐만 아니라 metagenome library를 이용한 새로운 방법의 개발이라 사료 된다. 또한 in vitro 스크리닝에서 얻어진 2종의 metagenome clone의 염기서열을 분석하여 항역병 활성에 관련하는 DNA 서열을 확보하고 이를 응용하여 물질을 생산 할 경우, 현장에서 활용 할 수 있는 효과 큰 친환경 천연고추역병 방제제로서의 개발 가능성을 가진다는 점에서 본 연구결과는 매우 의미 있는 결과라 생각된다. We have purified Phytophthora capsici alpha and beta tubulin from Escherchia coli BL21(DE3). The recombinant alpha and beta tubulins were assembled into microtubule in vitro with specific conditions. The metagenome library was isolated from soil in the Mt. Yeo-Ki, Suwon, Korea and manufactured with the method mentioned in experiment contents for in vitro screening of microtubule assembly screening. FRET effect was used for microtubule assembly inhibitor screening with metagenome library. We got 2 metagenome clones from in vitro screening, and these 2 hit clones showed P. capsici growth inhibition activity on the growing pepper plants. These results suggest that new development of potent inhibitor for pepper blight disease and new approach to prevention of pepper blight disease.

몽골 유래 Brucella melitensis 동정 및 특이 SNP를 이용한 real-time PCR법에 의한 진단 평가

강성일,김지연,김숙미,이진주,성소라,김연희,정석찬,허문,Kang, Sung-Il,Kim, Ji-Yeon,Kim, Suk Mi,Lee, Jin Ju,Sung, So-Ra,Kim, Yeon-Hee,Jung, Suk Chan,Her, Moon 대한수의학회 2015 大韓獸醫學會誌 Vol.55 No.2

A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at $67^{\circ}C$ unlike those for other Brucella species observed at $61^{\circ}C$. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.

Bacteriological and etiological investigation of bovine brucellosis in South Korea with importance on control strategies

이진주(Jin Ju Lee),성소라(So-Ra Sung),염은지(Eun Ji Yum),김성엽(Seong Yeob Kim),김시철(Si-Chul Kim),서윤정(Yoon-Jeong Seo),최정수(Jeong-Soo Choi),윤순식(Soon-Seek Yoon) 한국예방수의학회 2022 예방수의학회지 Vol.46 No.1

In Korea livestock farms breeding cattle have been suffering from re-emerging problems of Brucella (B.) abortus infection while steady decline of bovine brucellosis. Therefore, this study underscored the identification and association of etiological agent of brucellosis in cattle in South Korea. The incidence of brucellosis in cattle was analyzed by bacteriological and molecular methods in 187 brucellosis-suspicious farms of 11 regions between 2018 and 2020. Brucella isolates from various specimens were identified by Brucella-specific multiplex PCR. Epidemiological data were collected by local official veterinarians through history taking from farmers and animal data systems. In 230 of 560 cattle (40.9%) and 94 of 187 farms (50.3%), a total of 313 B. abortus were isolated from various specimens, the majority of isolates were from supramammary lymph node (41%). In epidemiological findings, the majority of positive cases were mainly caused by resurgence (43.7%) and unknown (37.2%). Of 94 positive cases isolated B. abortus, abortion in cattle infected by B. abortus occurred in 51 farms (54.3%) where led to resurgence in 30 farms and environmental survival of B. abortus in 9 farms. Consequently, these findings revealed the existence of etiological agent of bovine brucellosis in Korea, which still occurred at low levels in distinct regions where are allowed to call for persistent biosecurity. Thus, we highlight that brucellosis in Korea needs to take more effective control strategies with potential evidence. Moreover it is ultimately important to maintain a constant monitoring for eradication of brucellosis.