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      • KCI등재

        Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region

        Pil-Soo Seo(필수),Sook-Young Lee(이숙영),Yong Kook Shin(신용국) 한국생명과학회 2017 생명과학회지 Vol.27 No.3

        본 연구에서 밝혀진 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열은 NCBI (National Center for Biotechnology Information)의 GenBank에 Vigna sinensis AY195581로 등록하였다. ITS1, 5.8S 및 ITS2의 총 염기서열 507 염기서열을 이용한 Vigna sinensis (AY195581)의 분자계통분석에서 Vigna unguiculata 및 그 아종들과 98~100% 범위의 염기서열 상동성을 보였다. Vigna unguiculata는 계통분석에 이용된 다른 종들로부터 독립된 하나의 cluster로 그룹핑(grouping)이 됨을 확인하였다. 본 계통분석은 Vigna unguiculata가 Vigna 속의 다른 종에 비해 비교적 최근에 분화되었으며, 현재 유전적인 변화가 많이 일어나고 있음 보이고 있다. 또한, Vigna 속, Vigna longifolia, Vigna vexillata, Vigna membranacea, Vigna friesiorum, Vigna monophylla, Vigna schimperi, Vigna nigritia, Vigna lasiocarpa, Vigna trichocarpa, Vigna diffusa의 다른 종들과 비교하여 유전적으로 독립적인 종임을 확인하였다. 본 연구의 Vigna sinensis의 ITS1, 5.8S 및 ITS2를 이용한 계통분석은 Vigna sinensis를 Vigna unguiculata로 분류하는 것이 타당한 것으로 보여진다. 본 종은 국내에서 멸종된 것으로 알려져 있었으나 최근 토착 식물로써 발견되었고 이 갓끈동부의 관련 식물 종들과의 분자계통학적 위치를 명확히 밝힘에 의의가 있다고 하겠다. Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

      • KCI등재

        프로젝트형 거꾸로 교실이 예비 영어교사들의 정의적 태도 및 메타인지 학습전략에 미치는 효과

        송해성(Sohng HaeSung),서숙영(Seo SukYoung) 한국영어어문교육학회 2016 영어어문교육 Vol.22 No.2

        The flipped classroom is a learner-centered teaching and learning method, in which the traditional classroom model is reversed. When a classroom is reversed, instruction is delivered outside of class, usually in the form of video lectures and active learning takes place in class. In this study, project-based learning was combined with the flipped classroom to explore its effects on the affective attitude and metacognitive learning strategies of pre-service teachers of English (n=31). A survey of 25 items was given at the beginning and at the end of a 15-week semester during which subjects were taking a class of the project-based flipped classroom. The results show that the project-based flipped classroom had the strongest positive effect on increasing students’ confidence in learning, followed by participation and interest. However, no significant effects were found on students’ metacognitive learning strategies. Further, students were satisfied with the way the project-based flipped classroom worked. These results suggest that the project-based flipped classroom could be an effective method to improve students’ learning ability.

      • KCI등재
      • 급성 호산구성 폐렴 1례

        숙영,김형진,배용목,길동,황영훈,여동승,임성엽 대한알레르기학회 1999 천식 및 알레르기 Vol.19 No.3

        Acute eosinophilic pneumonia(AEP) was first described in 1989 and represents a clinical entity distinct from chronic eosinophilic pneumonia. AEP is characterized by acute respiratory insufficiency, hypoxemia, fever, diffuse radiographic infiltrates, and eosinophilia in bronchoalveolar lavage fluid or lung biopsies in the absence of infection, atopy, or asthma. Rapid response to corticosteroids is characteristic. We experienced a 47-year-old metal driller presenting typical clinical and radiological characteristics of AEP. We confirmed eosinophilic pneumonia with brochoalveolar lavage analysis and transbronchial lung biopsy. We report a case of AEP diagnosed by clinical, radiographic, and histologic findings with a brief review of the literature.

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