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TALP-32의 인체자궁암 세포주 HeLa에 대한 세포독성
박지훈(Ji-Hoon Park),김종석(Jong-Seok Kim),윤은진(Eun-Jin Yun),송경섭(Kyoung-Sub Song),서강식(Kang-Sik Seo),김훈(Hoon Kim),정연주(Yeon-Joo Jung),윤완희(Wan-Hee Yun),임규(Kyu Lim),황병두(Byoung-Doo Hwang),박종일(Jong-Il Park) 한국독성학회 2006 Toxicological Research Vol.22 No.4
TALP-32 is highly basic protein with a molecular weight of 32 kDa purified from human term placenta. Some basic proteins such as defensins and cecropins are known to induce cell death by increasing membrane permeability and some of them are under development as an anticancer drug especially targeting multi-drug resistant cancers. Therefore, we investigated cytotoxic effect and mechanism of TALP-32. When HeLa cell was incubated with TALP-32, cytotoxicity was increased in time and dose dependent manner. As time goes by, HeLa cells became round and plasma membrane was ruptured. Increase of plasma membrane permeability was determined with LDH release assay. Also in transmission electron microscopy, typical morphology of necrotic cell death, such as cell swelling and intracellular organelle disruption was observed, but DNA fragmentation and caspase activation was not. And necrotic cell death was determined with Annexin V/PI staining. The cytotoxicity of TALP-32 was minimal and decreased on RBC and Hep3B respectively. These data suggests that TALP-32 induces necrosis on rapidly growing cells but not on slowly growing cells implicating the possibility of its development of anticancer peptide drug.
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인체 대장암 세포주 SW480에서 docosahexaenoic acid에 의한 침윤억제 기전
Soyeon Shin(신소연),Yong-Jo Kim(김용조),Kyoung-Sub Song(송경섭),Kaipeng Jing,Nayeong Kim(김나영),Soyeon Jeong(정소연),Ji-Hoon Park(박지훈),Kang-Sik Seo(서강식),Jun-Young Heo(허준영),Hyun-Joo Kwon(권현주),Jong-Il Park(박종일),Seung 한국생명과학회 2010 생명과학회지 Vol.20 No.4
대장암은 미국 등 서양 국가뿐만 아니라 국내에서도 2번째로 많이 발병이 되는 암으로 알려져 있다. 역학조사에 의하면 ω3-PUFAs를 많이 섭취한 인종에서 대장암 발생빈도가 감소하고 최근 ω3-PUFAs는 수종의 암에 대해 항암작용을 나타낸다고 한다. 이에 본 연구에서는 대장암에서 DHA 등 ω3-PUFA의 항침윤 기전을 규명하여 다음과 같은 결과를 얻었다. DHA 및 EPA는 대장암 세포주 SW480의 증식을 농도 의존적으로 억제하였으나 AA는 거의 영향이 없었으며 TUNEL assay로 apoptotic cell death가 확인 되었다. DHA는 β-catenin 단백 및 TCF/LEF luciferase 활성을 농도 의존적으로 억제 하였다. SW480 세포의 침윤능은 DHA의 농도에 의존적으로 억제되었다. DHA처리 후 MMP-9 및 MMP-2 mRNA양이 감소되었을 뿐만 아니라 그 promoter의 reporter 활성도 억제되었다. NF-kB 및 p-IkB 단백짙양도 DHA의 처리농도에 의존적으로 감소하였으며 NF-kB promoter의 활성도 억제되었다. 이상의 결과로 ω3-PUFA는 대장암에서 NF-kB 신호전달 차단에 의한 MMP-2 및 MMP-9 발현을 억제하여 침윤을 억제하여 항암작용을 나타낼 수 있음을 시사하며, 따라서 ω3-PUFA는 대장암의 예방 및 치료에 유용하게 사용될 수 있으리라 생각된다. Colon cancer is one of the most common malignancies in the western world and the second leading cause of cancer death in Korea. Epidemiology studies have shown a reduced incidence of colon cancer among populations consuming a large quantity of ω3-polyunsaturated fatty acids (ω3-PUFA) of marine origin. Recently, it has been found that ω3-PUFA has an antineoplastic effect in several cancers. This study was designed to investigate the mechanism of the anti-invasive effect of ω3-PUFA in colon cancer. ω3-PUFA, docosahexaenoic acids (DHA) and eicosapentaenoic acid (EPA) treatment resulted in a dose-dependent inhibition of cell growth in SW480 human colon cancer cells. In contrast, arachidonic acid (AA), a ω6-PUFA, exhibited no significant effect. This action likely involves apoptosis, given that DHA treatment increased apoptotic cells in TUNEL assay. Moreover, invasiveness of SW480 cells was inhibited following treatment of DHA in a dose-dependent manner; in contrast, AA had no effect. The levels of MMP-9 and MMP-2 mRNA decreased after DHA pretreatment. MMP-9 and MMP-2 promoter activities were also inhibited by DHA treatment. The levels of NF-kB and p-IkB protein were down-regulated by DHA pretreatment in a dose dependent manner. In addition, DHA inhibited NF-kB promoter reporter activities. These findings suggest that ω3-PUFA may inhibit cancer cell invasion by inhibition of MMPs via reduction of NF-kB in colon cancer. In conclusion, ω3-PUFA could be used for chemoprevention and treatment of human colon cancer.
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서재천,서강식,조붕환,지옥화,장원순 공주교육대학교 2010 公州敎大論叢 Vol.47 No.1
본 연구는 교육과학기술부의 경쟁력있는 국립대학을 만들고자 시행하고 있는 여러 정책 중 연합대학으로의 체제 변화 권유에 대한 장단점과 이해득실을 냉철하게 객관적으로 고찰해 보고자 수행되었다. 연구의 수행방법은 연합대학 체제의 추진이나 독자생존의 길 등 의사결정에 참고자료가 될 수 있도록 일본과 미국 등 외국의 연합대학 사례를 중심으로 수집 가능한 자료를 수집하여 고찰한 후 연합대학 체제 추진이나 독자생존의 장단점을 알아보았다. 본 연구의 결과는 여러 가지 외적인 상황 속에서 우리 대학이 독자적으로 생존하는 것이 가능한가, 또는 결국에는 독자적 생존의 대안으로 연합 내지는 통합의 길을 선택할 수밖에 없는가 하는 것은 그 누구도 쉽게 예단하기 어렵다는 것이다. 그러나 대학 구성원들이 미래를 선택하지 않는다 하더라도 외적인 요인에 의해 미래가 결정될 여지가 있으므로 지금부터라도 대학의 미래에 관한 공동의 지혜를 모아야 할 것으로 생각한다. 함께 머리를 맞대고 허심탄회하게 서로의 의견을 존중하며 서로에 대한 불신을 털어버리고 진정 대학의 미래를 위한 바른 선택이 무엇인가에 관한 논의와 연구가 꾸준하게 지속되어야 할 것으로 판단된다. This study was carried out in order to try to consider objectively merits and demerits regarding a system change invitation toward the Union University System and understanding gains and losses during a lot of policies to make a national college that there was competitiveness of Ministry of Education, Science and Technology of education. The results of this study is that no one is easy to predict, that it does as alternatives of an independent survival or select a street of integration and the Union University System in various external situations, finally. However, the University members does not select the future even if in various external factor because there is no doubt that the future will be decided from now on any University in the future regarding collaborative wisdom of the university, We thought it was going to do. Head with open-minded to respect each other's opinions and distrust each other confide you discard the truly University for the future, select the right to discuss and research can be sustained.
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RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향
박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-
TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.
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