盧天命 硏究

白寅順 우석대학교 국어국문학연구회 1988 又石語文 Vol.5 No.-

엄마 아빠가 찍은 사진

백인순,고창자 새가정사 1979 새가정 Vol.- No.-

영어의 억양에 대하여

백인순 중앙대학교 사범대학 외국어 교육학회 1976 語文硏究 Vol.1 No.-

A Molecular Framework for Risk Assessment of a Virus- Tolerant Transgenic Pepper Line

백인순,김유진,육은수,이우규,윤원기,박기웅,김창기,한치학,김환묵,정순천 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.2

The Cucumber mosaic virus coat protein (CMV-CP) gene-transgenic pepper lines exhibit high tolerance to Cucumber mosaic virus (CMV) strains. In this study, E7, one of the CMVP0-CP transgenic chili pepper events selected by screening was further characterized. Southern blotting and inverse PCR analysis revealed that the E7 event contains a single copy of the inserted gene cassette whose flanking sequences appear to be noncoding and intergenic. We searched for pepper-specific DNA sequence candidates as an endogenous reference gene for GM-pepper detection. We found that only one copy of CaSIG4 and lipocalin genes are present in the pepper genome and their sequences were determined to be pepper-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the pepper-specific single copy CaSIG4 and lipocalin genes as endogenous reference genes, enabled the design of E7-event-specific PCR-based quantitative detection methods. The CMV-CP protein levels in the CMVinoculated wild-type pepper tissues were approximately 60 times higher than those in the uninoculated and CMV-inoculated E7 pepper tissues. These results suggested that the amount of CMV-CP expressed in transgenic pepper tissue was negligible relative to the amount of CMV-CP in the virus-infected wild-type pepper consumed by human beings. This work may prove useful for risk assessment studies of transgenic pepper lines. Furthermore, the characterized single copy genes, lipocalin and CaSIG4, may be used to develop a method to detect gene copy number variations in the pepper genome. The Cucumber mosaic virus coat protein (CMV-CP) gene-transgenic pepper lines exhibit high tolerance to Cucumber mosaic virus (CMV) strains. In this study, E7, one of the CMVP0-CP transgenic chili pepper events selected by screening was further characterized. Southern blotting and inverse PCR analysis revealed that the E7 event contains a single copy of the inserted gene cassette whose flanking sequences appear to be noncoding and intergenic. We searched for pepper-specific DNA sequence candidates as an endogenous reference gene for GM-pepper detection. We found that only one copy of CaSIG4 and lipocalin genes are present in the pepper genome and their sequences were determined to be pepper-specific. The characterization of the genomic sequences flanking the transgene, as well as the availability of the pepper-specific single copy CaSIG4 and lipocalin genes as endogenous reference genes, enabled the design of E7-event-specific PCR-based quantitative detection methods. The CMV-CP protein levels in the CMVinoculated wild-type pepper tissues were approximately 60 times higher than those in the uninoculated and CMV-inoculated E7 pepper tissues. These results suggested that the amount of CMV-CP expressed in transgenic pepper tissue was negligible relative to the amount of CMV-CP in the virus-infected wild-type pepper consumed by human beings. This work may prove useful for risk assessment studies of transgenic pepper lines. Furthermore, the characterized single copy genes, lipocalin and CaSIG4, may be used to develop a method to detect gene copy number variations in the pepper genome.

Roundup Ready Soybean의 정량 검출에서 SYBR Green I을 이용하는 Real-Time PCR 조건의 분석

백인순(In-Soon Pack),정순천(Soon-Chun Jeong),이규화(Kyu Hwa Lee),윤원기(Won Kee Yoon),박상규(Sangkyu Park),김창기(Chang-Gi Kim),김환묵(Hwan Mook Kim) 한국독성학회 2005 Toxicological Research Vol.21 No.3

Concerns on the safety of living modified (LM) crops have led to mandatory-labeling legislation of LM crops in many countries including Korea. An real-time PCR method for quantification of LM Roundup Ready soybean (RRS) with the double-stranded DNA intercalating dye, SYBR Green I, is described. Pairs of primers that specifically PCR-amplify the targeted 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and le1 gene sequences were selected by means of analysis of melting-curve plot of PCR products. Then, the specificity and general performance of the selected primer pairs were further increased by selecting optimum primer concentration by means of serial analyses of combination of different concentrations of each primer in the selected primer pairs. Using 1, 2, and 5% RRS test samples and applying the real-time PCR with SYBR Green I, mean values deviated from true values by 5.5 to 25%. The precision of the real-time PCR with SYBR Green I was comparable with that of the real-time PCR with TaqMan chemistry, which is widely used for the quantification of LM crops today. The results suggest that the real-time PCR with SYBR Green I, which does not require the high-cost dye labelling step for the initial primer or probe design, could be an alternative method for the quantification of LM Crops.

GM 콩 DNA와 단백질의 안정성에 대한 열, 압력 및 산 처리의 영향

백인순(In-Soon Pack),정순천(Soon-Chun Jeong),윤원기(Won-Kee Yoon),박상규(Sangkyu Park),육은수(Eun-Soo Youk),김환묵(Hwan-Mook Kim) 한국식품과학회 2004 한국식품과학회지 Vol.36 No.4

형질전환 events에서 elite event를 신속히 선발하는 방법 및 선발 event의 분석

정순천,백인순,김보민,김지홍,김유진,육은수,김창기,한지학 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

잠재적 안전성에 대한 논란으로 인하여 신규 개발되는 유전자변형작물은 고전적인 신품종과는 달리 환경 및 식품으로서의 안전성이 검증되어야 한다. 유전자변형작물의 안전성 평가는 개발되는 형질전환집단에서 선발된 하나의 elite event에 대해 이루어지는데, 본 평가 대상 elite event는 유전자의 삽입으로 인한 비의도적인 형질 변화가 최소화된 event이어야 한다. 분자유전학적으로 비의도적인 유전자 삽입 효과가 최소화되기 위해서는 단일 복제수의 도입유전자가 식물 유전체의 intergenic region에 삽입될 것을 요구한다. 본 연구의 목적은 배추좀나방 내성을 부여하는 CryIAc1 유전자가 형질 전환된 49 event의 양배추 형질전환집단에서 elite event를 선발하는 것이다. 먼저 도입유전자 cassette이 tandem repeat로 빈번히 삽입되는 현상에 착안하여 도입유전자 cassette의 양 말단에서 제작한 primer의 방향에 따른 모든 조합을 사용하여 PCR을 수행하였다. 본 삽입구조 분석을 통하여 49개 event 중 36개 event는 tandem repeat의 구조로 도입유전자가 2개 복제 수 이상 삽입되었음을 알 수 있었다. 선발된 13개 event에 대하여 Southern blot 분석을 실시한 결과 7개 event가 단일 복제수의 도입유전자를 가짐을 알 수 있었다. 마지막으로 7개 event에 대한 삽입위치를 inverse PCR 기법을 사용하여 해명한 결과는 3개 event에서 단일 복제수의 도입유전자가 양배추 genome의 intergenic region에 삽입되었음을 알 수 있었다. 결론적으로, 본 연구 결과는 형질전환시의 도입유전자가 다중 복제수로 삽입될 시에 tandem repeat로 빈번히 삽입되는 현상에 기초하여 대량의 형질전환집단에서 elite event를 신속히 선발하는 방법을 제시하였다. 선발된 event의 분자유전학적 분석이 현재 진행되고 있다.

Two-year field monitoring shows little evidence that transgenic potato containing ABF3 significantly alters its rhizosphere microbial community structure

남기정,김효정,남경희,백인순,김수영,김창기 한국생태학회 2017 Journal of Ecology and Environment Vol.41 No.3

Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional “Jopoong” plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.

Gene Flow from Transgenic PPO-inhibiting Herbicide-resistant Rice to Weedy Rice, and Agronomic Performance by Their Hybrids

남경희,김도영,문예슬,백인순,정순천,박기웅,김창기 한국식물학회 2019 Journal of Plant Biology Vol.62 No.4

Hybridization of transgenic crops with theirweedy relatives may spread transgenes into weedy populationsand alter the ecological fitness of such hybrids. Our studyobjective was to quantify the rate of gene flow from herbicideresistanttransgenic rice to weedy rice and compare theperformance of hybrids between them over several generations. The transgenic plants used in these experiments are resistant toprotoporphyrinogen IX oxidase (PPO)-inhibiting herbicidessuch as oxyfluorfen, oxadiazon, and butafenacil. The rate ofgene flow from those plants to five weedy accessions rangedfrom 0.025% to 0.139%. The F1 hybrids that resulted fromgene flow from transgenic to weedy rice and subsequent F2progeny were shorter than the weedy parents but producedsignificantly more and heavier grains. The F3 progenyresulting from gene flow were resistant to oxyfluorfen, whichindicated that the gene associated with PPO-inhibitingherbicide resistance is dominantly inherited. The GR50values (dose required for 50% shoot fresh weight reduction) forF3 progeny were >69-fold greater than for the weedy rice. These results suggest that transgenes that could escape fromPPO-inhibiting herbicide-resistant rice and be transferred toweedy rice might persist and disperse into weedy populationsover several generations due to herbicide resistance andimproved reproductive traits in the hybrids.

Bt 양배추가 복숭아혹진딧물의 발육과 기주선택에 미치는 영향

남기정,김영중,문두범,남경희,백인순,박정호,정순천,한지학,김창기,Nam, Ki Jung,Kim, Young-Joong,Moon, Doo-Bum,Nam, Kyong-Hee,Pack, In Soon,Park, Jung-Ho,Jeong, Soon-Chun,Harn, Chee Hark,Kim, Chang-Gi 한국응용곤충학회 2014 한국응용곤충학회지 Vol.53 No.2

해충저항성 유전자변형 작물은 대상 해충에 대한 방제 효과를 증진시킬 수 있는 효과적인 수단이 될 수 있지만, 생태계 내의 다른 비표적 생물에 부정적인 영향을 줄 가능성 또한 있기 때문에 상업적인 재배에 앞서 이에 대한 충분한 연구가 이루어져야 한다. 본 연구에서는 배추좀나방내성 유전자변형 Bt (Cry1Ac1)양배추가 비표적 곤충인 복숭아혹진딧물에 미치는 영향을 알아보기 위한 기초적인 실험으로서 기주선택실험 및 성장실험을 실시하였다. 기주선택실험에서, 두 개체씩의 Bt 양배추와 모본 양배추에 각각 10마리의 유시 성충(alate virginoparae)을 접종하고, 사흘간 생식(reproduction) 기회를 제공한 후 생산한 약충의 마리수를 측정한 결과, Bt 양배추와 모본 양배추에 낳은 약충의 수가 각각 $21.9{\pm}1.8$와 $22.5{\pm}2.2$로 비슷하였다. 성장실험에서, 갓 태어난 무시 약충(apterous virginoparae)을 Bt 양배추 및 모본 양배추를 기주로 하여 키웠을 때, 성충이 될 때까지 걸린 시간은 Bt 양배추와 모본 양배추에서 각각 $5.8{\pm}0.2$, $5.9{\pm}0.1$ 일(day)로 통계적으로 유의한 차이가 없었고, 개체군 생장률(rm) 또한 $0.7{\pm}0.1$, $0.8{\pm}0.1$로 유사하였다. 이상의 결과들로 볼때 Bt 양배추가 복숭아혹진딧물에 미치는 영향이 없거나 미미함을 알 수 있으나, 다양한 관점에서의 보완 연구가 더 필요하다. Transgenic crops that produce insecticidal toxins have a great potential for controlling target pest insects, but there is a growing concern about unintended influences on non-target species. In the present study, the preferences and performance of non-target green peach aphid, Myzus persicae (Sulzer), on transgenic cabbages (Brassica oleracea) that produce Bt toxin (Cry1Ac1) and untransformed control plants were investigated as a part of risk assessment. In a free-choice situation, the number of nymphs larviposited by 10 winged adults over 3 days was $21.9{\pm}1.8$ and $22.5{\pm}2.2$ on transgenic and the control plants, respectively, indicating that the aphids did not discriminate between the two types of plants. In a performance assay, the development time (D) and intrinsic rate of increase (rm) of wingless aphids reared on transgenic and control plants were also similar (D, $5.8{\pm}0.2$ and $5.9{\pm}0.1$ (days) and rm, $0.7{\pm}0.1$ and $0.8{\pm}0.1$, for transgenic and control plants, respectively). These results suggest that M. persicae is not significantly affected by transgenic Bt cabbage.