테라테인먼트적 코어운동프로그램과 간섭파 전기자극이 복부비만 여성에 미치는 영향

백윤웅(Yun-Woong Paek),김황용(Hwang-Young Kim) 한국엔터테인먼트산업학회 2017 한국엔터테인먼트산업학회논문지 Vol.11 No.4

본 연구는 20대 비만여성의 복부비만에 코어운동 프로그램과 간섭파 전기자극을 적용하여 그 효과를 알아보고자 하였다. 복부비만 여성 30명을 무작위로 선정하여 코어운동프로그램군과 간섭파 전기자극군, 그리고 대조군으로 나눴다. 코어운동프로그램군과 간섭파 전기자극군의 테라테인먼트중재는 각각 1회 30분, 주 3회, 6주간을 실시하였고 연구대상자들의 체질량지수, 복부둘레, 피하지방을 중재 전, 후에 측정하였다. 대응표본 T검정의 결과에서 코어운동프로그램군과 간섭파 전기자극군에서 체질량지수와 복부둘레에서 유의한 차이가 나타났고 일원배치분산분석후 사후검정에서도 코어운동프로그램군과 간섭파 전기자극군에서 유의한 차이가 나타났지만 피하지방에서는 세군 모두 유의한 차이가 나타나지 않았다. 그러므로 향후 연구에서는 비만을 가진 다양한 그룹을 대상으로 테라테인먼트적 전기자극을 제공하여 객관적인 평가를 통한 검증을 하고 더 나아가 가정용 전기자극 건강보조기구 개발이 필요할 것으로 보인다. This research investigates the effects of theratainment core exercises and theratainment interferential current on abdominal obesity for women in their twenties. A total of thirty female participants with abdominal obesity were randomly divided into three groups: Group A participated in a theratainment core exercise program, Group B received theratainment interference electrical stimultation, and Group C was designed as the control group. The theratainmnet intervention for the Group A and B were conducted for six weeks, thirty minutes per day, three days per week. BMI, waistline, and skin-fold thickness data were taken as pretest and posttest measures. The results of the paired-t test showed that there were significant differences in both BMI and waistline for participants involved in the theratainment core exercise program as well as thertainment interferential current(p<.05). In addition, there were significant differences in the core exercise program group and the interferential current group in post-hoc test from one-way ANOVA. There were significant differences in BMI and waistline measures within both experiment groups. However, no difference was observed in the skin-fold thickness measurement. For future studies, it is suggested that researchers should control participants’ eating habits, apply electrical stimulation to many abdominal muscles, and employ objective measurements.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 냉치료에 의한 비복근의 iNOS의 감소

백윤웅(Paek Yun-Woong),한상완(Han Sang-Wan) 한국코칭능력개발원 2008 코칭능력개발지 Vol.10 No.1

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 경피신경전기자극과 냉치료에 의한 척수 cyclooxygenase-2의 감소

백윤웅(Yun-Woong Paek),채윤원(Yun-Won Chae),김정우(Jung-Woo Kim) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.5

근육통에 의해 유발된 이차성 통각과민과 척수의 허리팽대부에서의 cyclooxygenase-2 (COX-2)의 발현에 경피신경전기 자극(transcutaneous electrical nerve stimulation, TENS)과 냉치료에 대한 효과를 알아보기 위하여 연구되었다. 흰쥐의 장딴지근에 2% carrageenan을 근육주사하여 근통증 실험모델을 만들었다. 이차성 통각과민을 측정하기 위하여 열에 대한 발움추림잠복기 (paw withdrawal latency, PWL)와 꼬리치기검사 (tail flick latency, TFL)를 조사하였다. 척수의 허리팽대부의 COX-2 발현을 역전사중합연쇄반응 (reverse transcription-polymerase chain reaction, RT-PCR)과 면역조직화학염색법에 의해 조사하였다. 열에 대한 이차성 통각과민은 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 유의적으로 감소하였다. 척수의 허리팽대부에서 COX-2 mRNA 수준은 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 감소하였으며 또한, COX-2 면역반응성도 척수의 뒤뿔에서 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 감소하였다. 이러한 결과들은 경피신경전기자극과 냉치료를 적용한 그룹에서 COX-2 발현의 감소는 근육통에 의해 유발된 이차성 통각과민을 감소시키는데 중요한 요인으로 작용할 가능성이 있음을 시사한다. The purpose of this study was to investigate the effects of transcutaneous electrical nerve stimulation (TENS) and cold therapy on cyclooxygenase-2 (COX-2) expression in lumbar spinal cord and on secondary hyperalgesia produced by muscle pain. Muscle pain was experimentally induced by injection of 2% carrageenan into gastrocnemius muscle of Sprague-Dawley rats. For assessment of secondary hyperalgesia, the rats were tested for paw withdrawal latency (PWL) and tail flick latency (TFL) to heat stimulus. COX-2 expression in lumbar cord was examined using RT-PCR and immunohistochemistry. Secondary hyperalgesia to heat stimulus was significantly reduced in the both TENS- and cold-treatment groups, compared to the control group. The COX-2 mRNA levels were down-regulated in the lumbar spinal cord in the both TENS- and cold-treatment groups. In addition, COX-2 immunoreactivity was decreased in the dorsal horn of the lumbar spinal cord in the both TENS- and cold-treatment groups. These results suggested that decreased COX-2 expression in the lumbar spinal cord of the subjects receiving TENS treatment and cold therapy might be an important factor for reducing secondary hyperalgesia produced by muscle pain.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 냉치료에 의한 척수의 c-fos의 발현

백윤웅,Paek Yun-Woong 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.4

Expression of c-fos, an immediate early gene, has accepted to be a marker of functional activity in neurons. This study was aimed to investigate the effects of cold therapy on the expression of spinal cord c-fos in rats of carrageenan-induced muscle pain. Muscle pain was induced in male Sprague-Dawley rats by intra-muscular injection of gastrocnemius with $2\%$ carrageenan. The paw withdrawal latency (PWL) and tail flick test (TFT) responses to heat stimuli were used to detect secondary hyperalgesia produced by the muscle pain and measured to assess the effects of cold. The expression of c-fos was determined in the lumbar regions of the spinal cord by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. The secondary hyperalgesia to heat simuli (PWL and TFT) were significantly reduced in cold therapy compared with that in the controls. In RT-PCR assays the expression of c-fos mRNA was down-regulated in the lumbar spinal cord in cold group. In addition, Fos immunoreactivity in the dorsal horn of the lumbar spinal cord was decreased in cold group. These results suggested that application of cold attributed to increase PWL and TFT responses and to decrease expression of the c-fos produced by muscle pain.

과당식이 고혈압 흰쥐에서 혈관 Endothelin-1과 산화질소합성효소의 발현

백윤웅,김명훈,Paek, Yun-Woong,Kim, Myung-Hoon 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.4

Rats that are fed a fructose-rich diet develop hypertension, insulin resistance, and hypertriglyceridemia. To elucidate whether altered expression levels of endothelin-1 and nitric oxide synthase are related to the development of insulin-resistant hypertension, we examined the present study. Male Sprague-Dawley rats were fed a fructose-rich diet for 5 weeks. Systolic blood pressure significantly increased in fructose-fed rats. While serum free fatty acid and plasma nitrite/nitrate levels did not significantly differ between the fructose-fed and control groups, plasma insulin and serum triglyceride concentrations significantly increased in the former. Endothelin-1 mRNA expression in the aorta increased in fructose-fed rats. Neither the protein expression of constitutive nitric oxide synthase nor that of inducible nitric oxide synthase were significantly affected by fructose feeding. However, nitrite/nitrate levels in the aorta were significantly increased. These results suggest that an increase in vascular endothelin-1 is an important contributing factor to the development of hypertension in fructose-fed rats. However, the vascular nitric oxide pathway may not be causally related to the development of fructose-induced hypertension.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 경피신경전기자극과 냉치료에 의한 비복근의 cyclooxygenase-2의 감소

백윤웅,채윤원,Paek, Yun-Woong,Chae, Yun-Won 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.1

Prostaglandins are generated through two isoforms of the enzyme cyclooxygenase, constitutively expressed cyclooxygenase(COX)-1 and COX-2, which is induced at sites of inflammation. Inhibition of COX-2 is desirable as this may avoid side effects seen with NSAIDs. We examined the effects of transcutaneous electrical nerve stimulation and cold therapy on the levels of muscle cycloooxygenase-2 mRNA in rats of carrageenan-induced inflammatory. The method of behavioral assessment were paw withdrawal latency(PWL) and tail flick test(TFT). The COX-2 mRNA levels were quantified by reverse transcription-polymerase chain reaction (RT-PCR). Following the transcutaneous electrical nerve stimulation and cold therapy, PWL and TFT were increased and COX-2 mRNA expression in gastrocnemius muscles were decreased. These results suggest that a transcutaneous electrical nerve stimulation and cold therapy were good therapy for a muscle pain.

Decreased Nitric Oxide Synthesis in Rats with Chronic Renal Failure

나명윤(Myong Yun Nah),김수완(Soo Wan Kim),이종은(Jong Un Lee),백윤웅(Yun Woong Paek),강대길(Dae Gill Kang),최기철(Ki Chul Choi),강영준(Young Joon Kang) 대한신장학회 2000 춘계학술대회 초록집 Vol.2000 No.-

N/A

복합관절 운동 형태의 등장성 운동과 등속성 운동이 하지근력 및 기능수행에 미치는 효과

한상완(Han Sang-Wan),백윤웅(Paek Yun-Woong),김성수(Kim Sung-Soo) 한국코칭능력개발원 2010 코칭능력개발지 Vol.12 No.1

양측 요로 폐쇄 흰쥐에서 신장 ANP 계 활성 변화

박종욱(Jong Wook Park),백윤웅(Yun Woong Paek),염충호(Chung Ho Yeum),나명윤(Myong Yun Nah),김수완(Soo Wan Kim) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.1

N/A Background: Although being associated with an elevated plasma atrial natriuretic peptide(ANP), its precise role in the postobstructive diuresis has not been fully under- stood. Evidence has been provided suggesting that the locally-synthesized ANP in the kidney contributes to the regulation of urinary sodium excretion. The present study was aimed to investigate whether an altered regulation of local ANP system is involved in the postobstructive diuresis. Methods: Male Sprague-Dawley rats were used. Both proximal ureters were ligated for 48 hours, after which the kidneys were taken without releasing the ligature, being designated as bilateral ureteral obstruction(BUO) group; or the ligature was released and 4 or 24 hr later, urinary data were collected, being designated as BUR-4 or BUR-24, respectively. Sham operated rats were used as control. Plasma ANP levels were determined by radioimmunoassay. The expression of ANP and natriuretic peptide receptor(NPR)-A mRNAs was determined by reverse transcription-polymerase chain reaction(RT-PCR). To further examine whether the altered renal ANP system, if any, was associated with an altered biological effects of guanylyl cyclase, ANP- stimulated cGMP accumulation was determined in membrane preparations of the glomeruli and papillae by radioimmunoassay. Results: The plasma ANP level was increased in BUO group compared with that in the control(260.5±32.5 vs. 133.3±23.5pg/mL, p<0.05), decreased in BUR-4 group(3.6±0.5 vs. 143.5±42.8pg/mL, p<0.01), while not significantly different in BUR-24 group. In BUR- 4. the urinary flow rate increased compared with that in the control(1598±370 vs. 215<34 DμL/hr, p<0.01), along with increases of FENa(11.5±64.1 vs. 0.25±0.02%, p<0.05) and UNaV (153.7±23.7 vs. 36.5±9.3 μEq/hr, p<0.01). In BUR-24, the urinary parameters were normalized. Renal tissue expression of ANP mRNA was increased in BUO as well as in BUR-4, while not changed in BUR-24. NPR-A mRNA expression was decreased in the kidney of BUO. The ANP-stimulated accumulation of cGMP in the isolated glomeruli and papillae in BUO was significantly reduced. The guanylyl cyclase activities were partly recovered in BUR-4 and completely in BUR-24. Conclusion : An enhanced local activity of ANP in the kidney may be causally related to the postobstructive diuresis.

유용 2차 대사산물의 (代謝産物) 생산을 위한 약용식물의 증식과 형질전환 - 1. 기내배양을 (器內培養) 통한 병풀 엽병의 식물체 재분화

김광수(Kwang Su Kim),백윤웅(Yun Woong Paek),고경민(Kyung Min Ko),황성진(Sung Jin Hwang),임병탁(Hyun Tag Im),황백(Baik Hwang) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2

Somatic embryogenesis and subsequent plantlet formation were obtained from embryogenic calli derived from young petioles of Centella asiatica. Embryogenic callus cultures were induced from petiole explants of Centella asiatica on basal MS medium supplemented with 1.0 ㎎/L 2,4-D and 1.0 ㎎/L kinetin. After transfer of these calli to MS medium supplemented with 5.0 ㎎/L NAA and 1.0 ㎎/L kinetin, 87% of embryogenic calli were differentiated into embryoids with primary and adventitious roots, elongated hypocotyl and cotyledon. Plantlets were induced when these embryoids were transferred to half strength inorganic salts of basal MS supplemented with 0.2 ㎎/L NAA and 0.2 ㎎/L kinetin.