Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 냉치료에 의한 척수의 c-fos의 발현

백윤웅,Paek Yun-Woong 대한물리치료학회 2003 대한물리치료학회지 Vol.15 No.4

Expression of c-fos, an immediate early gene, has accepted to be a marker of functional activity in neurons. This study was aimed to investigate the effects of cold therapy on the expression of spinal cord c-fos in rats of carrageenan-induced muscle pain. Muscle pain was induced in male Sprague-Dawley rats by intra-muscular injection of gastrocnemius with $2\%$ carrageenan. The paw withdrawal latency (PWL) and tail flick test (TFT) responses to heat stimuli were used to detect secondary hyperalgesia produced by the muscle pain and measured to assess the effects of cold. The expression of c-fos was determined in the lumbar regions of the spinal cord by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. The secondary hyperalgesia to heat simuli (PWL and TFT) were significantly reduced in cold therapy compared with that in the controls. In RT-PCR assays the expression of c-fos mRNA was down-regulated in the lumbar spinal cord in cold group. In addition, Fos immunoreactivity in the dorsal horn of the lumbar spinal cord was decreased in cold group. These results suggested that application of cold attributed to increase PWL and TFT responses and to decrease expression of the c-fos produced by muscle pain.

인간의 meningioma에서 nm23-H1의 발현과 세포이동능은 역비례한다

백윤웅 광주보건대학 2002 論文集 Vol.27 No.-

The ㎚23 gene is widely documented as a suppressor of the metastatic phenotype in many human cancers. However, its role with respect to the invasive behavior of primary central nervous system tumors has scarcely been addressed. The present study was aimed to examine the expression of the ㎚23-Hl gene product in 14 meningiomas in order to clarify the association of its expression with the histological types or migration rate, The ㎚23-Hl protein levels in human meningiomas were analyzed using Western blotting analyses. The ㎚23-Hl protein levels and cell migration rate was not correlated with different histologic types of meningiomas. However, the expression of ㎚23-Hl is inversely correlated with the migration rate in meningiomas. These results suggest that ㎚23-Hl may play an important role in the cell migration of meningiomas in female patients.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 경피신경전기자극과 냉치료에 의한 비복근의 cyclooxygenase-2의 감소

백윤웅,채윤원,Paek, Yun-Woong,Chae, Yun-Won 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.1

Prostaglandins are generated through two isoforms of the enzyme cyclooxygenase, constitutively expressed cyclooxygenase(COX)-1 and COX-2, which is induced at sites of inflammation. Inhibition of COX-2 is desirable as this may avoid side effects seen with NSAIDs. We examined the effects of transcutaneous electrical nerve stimulation and cold therapy on the levels of muscle cycloooxygenase-2 mRNA in rats of carrageenan-induced inflammatory. The method of behavioral assessment were paw withdrawal latency(PWL) and tail flick test(TFT). The COX-2 mRNA levels were quantified by reverse transcription-polymerase chain reaction (RT-PCR). Following the transcutaneous electrical nerve stimulation and cold therapy, PWL and TFT were increased and COX-2 mRNA expression in gastrocnemius muscles were decreased. These results suggest that a transcutaneous electrical nerve stimulation and cold therapy were good therapy for a muscle pain.

과당식이 고혈압 흰쥐에서 혈관 Endothelin-1과 산화질소합성효소의 발현

백윤웅,김명훈,Paek, Yun-Woong,Kim, Myung-Hoon 대한물리치료과학회 2002 대한물리치료과학회지 Vol.9 No.4

Rats that are fed a fructose-rich diet develop hypertension, insulin resistance, and hypertriglyceridemia. To elucidate whether altered expression levels of endothelin-1 and nitric oxide synthase are related to the development of insulin-resistant hypertension, we examined the present study. Male Sprague-Dawley rats were fed a fructose-rich diet for 5 weeks. Systolic blood pressure significantly increased in fructose-fed rats. While serum free fatty acid and plasma nitrite/nitrate levels did not significantly differ between the fructose-fed and control groups, plasma insulin and serum triglyceride concentrations significantly increased in the former. Endothelin-1 mRNA expression in the aorta increased in fructose-fed rats. Neither the protein expression of constitutive nitric oxide synthase nor that of inducible nitric oxide synthase were significantly affected by fructose feeding. However, nitrite/nitrate levels in the aorta were significantly increased. These results suggest that an increase in vascular endothelin-1 is an important contributing factor to the development of hypertension in fructose-fed rats. However, the vascular nitric oxide pathway may not be causally related to the development of fructose-induced hypertension.

nm23-H1 유전자가 주입된 U87MG 세포의 이동능과 침윤능의 감소

백윤웅 대한기초간호자연과학회 2005 Journal of korean biological nursing science Vol.7 No.1

nm23-H1 gene expression has been inversely correlated with tumor metastatic potential in certain tumors including melanomas, breast carcinomas, and hepatocellular carcinomas. However, its role with respect to the invasive behavior of central nervous system tumors has scarcely been addressed. Because cell motility and invasion plays an essential role in metastatic dissemination, we have studied whether motile human glioma cell(U87MG) transfected with nm23-H1 complementary DNA have any alterations in their ability to migrate and invade. There was no significant changes in the shape and size of the cells following nm23-H1 transfection. The role of nm23-H1 in glioma migration and invasion have been evaluated by in vitro simple scratch technique and brain slice invasion model. Basal migration ability of nm23-H1 transfectants cell(U87MG-pEGFP-nm23) were lesser than U87MG. Accordingly, U87MG-pEGFP-nm23 didn't migrate away apparently from the tumors implanted site comparing U87MG in brain slice invasion model. These results suggest that nm23-H1 may play an important role in suppressing the human glioma migration and invasion.

필라테스 운동이 폐 기능, 자기 효능감, 집중력에 미치는 영향

백윤웅 한국보건기초의학회 2015 한국보건기초의학회지 Vol.8 No.2

스트레칭과 PMT 적용에 따른 요통환자의 통증, 근력, 유연성의 변화

백윤웅,민순,이병훈,신명기 기초간호학회 2014 Journal of korean biological nursing science Vol.16 No.1

요통은 사람이 살아가면서 흔히 발생하는 증상으로, 대부분의 사람들은 살면서 한 번 이상의 허리통증을 겪는다고 알려져 있다(Park, Lee, & Lee, 2005). 이러한 요통은 척주를 둘러싼 인대나 근육, 관절 및 허리 운동과 관련된 근육들의 긴장이나 피로 또는 약화에 따라 발생하는 것으로 알려져 있다(Michael, 2002). 통증의 지속 시간에 따라 6주 이하면 급성, 6주에서 12주 사이를 아급성, 12주 이상일 경우 만성으로 분류한다(Wheeler, 1995). 급성인 경우는 대부분이 2개월 내에 호전을 보이는 반면 재발하는 경우가 많고 그 중의 10%는 만성요통으로 진행하게 된다.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 경피신경전기자극과 냉치료에 의한 척수 cyclooxygenase-2의 감소

백윤웅(Yun-Woong Paek),채윤원(Yun-Won Chae),김정우(Jung-Woo Kim) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.5

근육통에 의해 유발된 이차성 통각과민과 척수의 허리팽대부에서의 cyclooxygenase-2 (COX-2)의 발현에 경피신경전기 자극(transcutaneous electrical nerve stimulation, TENS)과 냉치료에 대한 효과를 알아보기 위하여 연구되었다. 흰쥐의 장딴지근에 2% carrageenan을 근육주사하여 근통증 실험모델을 만들었다. 이차성 통각과민을 측정하기 위하여 열에 대한 발움추림잠복기 (paw withdrawal latency, PWL)와 꼬리치기검사 (tail flick latency, TFL)를 조사하였다. 척수의 허리팽대부의 COX-2 발현을 역전사중합연쇄반응 (reverse transcription-polymerase chain reaction, RT-PCR)과 면역조직화학염색법에 의해 조사하였다. 열에 대한 이차성 통각과민은 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 유의적으로 감소하였다. 척수의 허리팽대부에서 COX-2 mRNA 수준은 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 감소하였으며 또한, COX-2 면역반응성도 척수의 뒤뿔에서 대조군에 비교하여 경피신경전기자극과 냉치료를 적용한 그룹에서 감소하였다. 이러한 결과들은 경피신경전기자극과 냉치료를 적용한 그룹에서 COX-2 발현의 감소는 근육통에 의해 유발된 이차성 통각과민을 감소시키는데 중요한 요인으로 작용할 가능성이 있음을 시사한다. The purpose of this study was to investigate the effects of transcutaneous electrical nerve stimulation (TENS) and cold therapy on cyclooxygenase-2 (COX-2) expression in lumbar spinal cord and on secondary hyperalgesia produced by muscle pain. Muscle pain was experimentally induced by injection of 2% carrageenan into gastrocnemius muscle of Sprague-Dawley rats. For assessment of secondary hyperalgesia, the rats were tested for paw withdrawal latency (PWL) and tail flick latency (TFL) to heat stimulus. COX-2 expression in lumbar cord was examined using RT-PCR and immunohistochemistry. Secondary hyperalgesia to heat stimulus was significantly reduced in the both TENS- and cold-treatment groups, compared to the control group. The COX-2 mRNA levels were down-regulated in the lumbar spinal cord in the both TENS- and cold-treatment groups. In addition, COX-2 immunoreactivity was decreased in the dorsal horn of the lumbar spinal cord in the both TENS- and cold-treatment groups. These results suggested that decreased COX-2 expression in the lumbar spinal cord of the subjects receiving TENS treatment and cold therapy might be an important factor for reducing secondary hyperalgesia produced by muscle pain.

2일 간격의 교대근무가 요중 17-OHCS와 Free Cortisol 농도에 미치는 효과

백윤웅,임욱빈,민순 성인간호학회 1997 성인간호학회지 Vol.9 No.3

In this study, 20 students in C university were selected by control group and 14 shift work nurses working in C university hospital by test group. It took part in case of day shift(8AM-4PM), evening shift(4PM-12MN), and night shift (12MN-8AM) to know a urinary 17-OHCS and free cortisol changes which works in a rapidly rotating shift work system. The working team were an rest period of a day and an observation of a week. It obtained an urine specimen before and after work shift in 2nd day. Test group and control group of shift work compared to change of levels of urinary 17-OHCS and free cortisol of urine collected from nurses of day shift, evening shift, and night shift. The data was analyzed by t-test, paired t-test. The results are as follows. 1. Compare 8AM with 4PM in day shift. Control group increased 4.1mg at 8AM, 4.2mg at 4PM in a case of 17-OHCS(p=.84) and also test group increased a little 3.5mg at start time of work, 3.6mg at stop time(p=.97). In a case of free cortisol control group decreased 3.8㎍ at 8AM, 2.4㎍ at 4PM(p=.12) and test group decresed 8.3㎍ at start time of work, 3.2㎍ at stop time(p=.22). 2. Compare 4PM with 12MN in evening shift. Control group decreased 4.2mg at 4PM, 2.9mg at 12MN in a case of 17-OHCS(p=.54), but test group increased 1.7mg at start time of work, 3.4mg at stop time(p=.07). In a case of free cortisol control group decreased 2.4㎍ at 4PM, 1.9㎍ at 12MN(p=.23) and also test group decresed a little 2.6㎍ at start time of work, 1㎍ to stop time(p=.43). 3. Compare 12MN with 8AM in night shift. Control group increased 3.9mg at 12MN, 4.1mg at 8AM in a case of 17-OHCS(p=.79) and also test group increased 6.3mg at start time of work, 8.4mg at stop time(p=.16). In a case of free cortisol control group increased 1.9㎍ at 12MN, 3.8㎍ at 8AM(p=.08) and test group increased 4.4㎍ at start time of work, 11.6㎍ at stop time(p=.04). As a result of this study reveals that health level of nurses is decreased in a rotating shift work(2days interval). As mentioned above, it was confirmed that the rotating shift work had influence on the health of the nurses. For it, I present the objective base data to mesure the level healthy in order to extent the understanding of the physical aspects of the nurses. Moreover, it is considered that one can make use of it as an objective base with a view to the rational management for the nursing administration.

Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 냉치료에 의한 비복근의 iNOS의 감소

백윤웅(Paek Yun-Woong),한상완(Han Sang-Wan) 한국코칭능력개발원 2008 코칭능력개발지 Vol.10 No.1