http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
감자 Sucrose Transporter 유전자의 벼 Genome 내로의 도입
백소현,유남희,윤성중 한국식물생명공학회 2001 식물생명공학회지 Vol.28 No.2
The transport and allocation of photosynthetic assimilate is an important regulatory factor in plant productivity, In order to modify assimilate partitioning in rice, transgenic plants containing a potato sucrose transporter (SuT) gene were developed. Calli derived from rice seeds (Oryza sativa L. cv Dongjin) were cocultured with A. tumefaciens LBA 4404 harboring the SuT gene. Calli were transferred to MS medium supplemented with 50 mg/L hygromycin, 500 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA. After 2 weeks, hygromycin resistant shoots were obtained from the calli on the selection medium. Roots were induced from the putative transgenic shoots on rooting MS medium supplemented with 250 mg/L cabenicillin. Plant regeneration rate from the calli was about 150%. Stable incorporation of the potato SuT gene into rice genomic DNA was confirmed by PCR and Southern blot analysis. 동화산물의 분배 효율 개선을 통한 생산성 향상 가능성을 조사하고자 감자의 sucrose 수송자 유전자를 벼에 형질전환 하였다. 동진벼로부터 유도된 callus를 감자의 sucrose 수송자 유전자가 도입된 A. tumefaciens LBA 4404와 공동배양한 후, 선발배지에서 증식된 callus를 250 mg/L carbenicillin, 2 mg/L kinetin, 0.1 mg/L NAA가 첨가된 MS 배지에 옮겨 약2주 후부터 소식물체를 얻었다. Carbenicillin 250 mg/L 첨가된 MS 기본 배지에서 소식물체의 발근을 유도하여 재분화 식물체를 얻었다. 선발된 callus는 약 150%의 높은 식물체 재분화율을 보였다. 재분화 식물체에 대한 PCR 분석을 수행하여 감자의 sucrose수송자 유전자가 삽입된 형질전환 벼 식물체를 선발하였다. 선발된 형질전환 식물체에 대한 Southern blot 분석을 통하여 외래유전자인 감질 sucrose 수송자 유전자가 벼 genome 내에 안정적으로 도입되었음을 확인하였다.
대학생들의 취업스트레스가 외모관심도와 외모관리 행동에 미치는 영향
백소현,장창곡 한국미용학회 2019 한국미용학회지 Vol.25 No.4
The purpose of this study was to examine the influence of job-seeking stress on appearance concerns and appearance management behaviors in college students. To achieve the purpose of this study, a survey was conducted on 228 students in universities located in Seoul, Gyeonggi Province and Cheonan. The findings of the study were as follows: First, the job-seeking stress of the college students was not that heavy, and they didn't show much appearance concerns or appearance management behaviors, either. The students who were in the higher academic year were under heavier job-seeking stress, and fourth grade students were particularly interested in appearance, and the female students showed more appearance concerns and expressed more satisfaction with their appearances than the male students. Second, job-seeking stress had a significant positive correlation with other orientation, activeness for better appearance, appearance management, appearance concerns, hair management behaviors, makeup behaviors, cosmetic-surgery management behaviors, skincare behaviors, body-shape management behaviors, fashion management behaviors and appearance management behaviors. Finally, jobseeking stress exerted a statistically significant positive influence on other orientation, activeness for better appearance, appearance management, appearance concerns, hair management behaviors, makeup behaviors, cosmetic-surgery management behaviors, skincare behaviors, body-shape management behaviors, fashion management behaviors and appearance management behaviors. Given the findings of the study, information on how to build a positive external image and how to take care of one's appearance should be provided to alleviate the job-seeking stress of college students because job-seeking stress affects appearance factors.
백소현,박성지,김민지,최연현,김성목 대한자기공명의과학회 2017 Investigative Magnetic Resonance Imaging Vol.21 No.1
Purpose: To evaluate quantification results of single breath-hold (SBH) magnetic resonance (MR) cine imaging compared to results of conventional multiple breathhold (MBH) technique for left ventricular (LV) function in patients with cardiac arrhythmia. Materials and Methods: MR images of patients with arrhythmia who underwent MBH and SBH cine imaging at the same time on a 1.5T MR scanner were retrospectively reviewed. Both SBH and MBH cine imaging were performed with balanced steady state free precession. SBH scans were acquired using temporal parallel acquisition technique (TPAT). Fifty patients (65.4 ± 12.3 years, 72% men) were included. End-diastolic volume (EDV), end-systolic volume (ESV), stroke volume(SV), ejection fraction (EF), myocardial mass, and LV regional wall motion were evaluated. Results: EF, myocardial mass, and regional wall motion were not significantly different between SBH and MBH acquisition techniques (all P-values > 0.05). EDV, ESV, and SV were significant difference between the two techniques. These parameters for SBH cine imaging with TPAT tended to lower than those in MBH. EF and myocardial mass of SBH cine imaging with TPAT showed good correlation with values of MBH cine imaging in Passing-Bablok regression charts and Bland-Altman plots. However, SBH imaging required significantly shorter acquisition time than MBH cine imaging (15 ± 7 sec vs. 293 ± 104 sec, P < 0.001). Conclusion: SBH cine imaging with TPAT permits shorter acquisition time with assessment results of global and regional LV function comparable to those with MBH cine imaging in patients with arrhythmia.
응급실을 방문한 급성 충수염환자의 진통 조절과 이에 영향을 미치는 요인 분석: 소아와 성인 비교 분석
백소현,김한범,곽영호,김도균,정재윤,서동범,이진희,정진희 대한응급의학회 2014 대한응급의학회지 Vol.25 No.4
Purpose: Appendicitis is one of the most common causes of acute abdominal pain presenting to the emergency department (ED). Emergency physicians are responsible for correct diagnosis and management of pain associated with appendicitis before surgery. We analyzed the comparison of management and pain control for appendicitis between adults and children in the ED. Methods: This study was a retrospective case control study, conducted in two EDs of a teaching hospital from 2011 to 2012. Patients who were diagnosed as appendicitis based on the radiologic modality were enrolled. We analyzed the clinical characteristics of the patients, who were adults and children diagnosed with appendicitis, respectively. We also analyzed the predictors of pain control for the appendicitis patients by multiple logistic regression. Results: A total of 2,130 patients were enrolled during a two-year period. The median age of the patients was 33 years (IQR 19~47) and 22.8% of patients were under 18 years of age; 54.6% were men. Ultrasonography was performed on 10.8% of patients and computed tomography (CT) on 89.4%. Significant difference in overall pain management was observed between children and adults (adult 29.5% vs children 20.6%, p=0.001). Adult patients younger than 65 years old, male, and those who underwent CT scan received more analgesics than others. Significant difference of the type of analgesics was observed between adults patients and pediatric patients. The use of analgesics did not affect the outcome, such as complication, unplanned revisit. Conclusion: Both adults and children with acute appendicitis still did not receive enough pain medication in the ED, even after being diagnosed.