Comet 분석을 통한 방사선처리 고추세포의 핵 DNA 손상평가

안정희,백명화,김재성,정정학,권순태,An, Jung-Hee,Back, Myung-Hwa,Kim, Jae-Sung,Jeong, Jeong-Hag,Kwon, Soon-Tae 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to $^{60}$ CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay. 방사선에 노출된 고추유묘의 잎으로부터 세포핵을 분리하여 단세포전기영동방법인 comet 분석을 통하여 핵 DNA의 손상정도를 조사하였다. Comet 분석에서 꼬리부분의 길이 (T)와 머리부분의 길이 (H)를 측정하여 T/H 비율을 조사하였다. 무처리세포는 T/H 비율이 1.28이었으나 50 및 100 Gy의 방사선을 처리한 세포는 각각 3.54 및 3.39로 방사선처리에 의해 상당량의 핵 DNA가 손상을 입은 것으로 나타났다. Comet의 head-DNA량은 무처리가 76.8%였으나 50 및 100 Gy를 처리한 세포는 각각 55.9% 및 59.5%를 보였다. 고선량의 방사선을 처리하기 전에 미리 20 Gy 이하의 저선량 방사선을 종자에 전처리하였을 경우 종자의 발아 및 생장에 대한 영향은 없었지만, 후속 고선량에 대한 핵 DNA의 손상은 경감되는 경향을 보였다.

저선량 ${\gamma}$선이 기냐 생산된 감자 소괴경의 휴면과 생에 미치는 효과

김동희,백명화,전재흥,김재성,Kim, Dong-Hee,Back, Myung-Hwa,Jeon, Jae-Heung,Kim, Jae-Sung 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4

저선량 ${\gamma}$선 조사가 기내 유기한 대지와 수미 감자 소괴경의 맹아율과 식물체 생장 및 생리활성에 미치는 효과 등을 알아보고자 감자 소괴경의 저장기간별로 ${\gamma}$선을 0.5${\sim}$30Gy 까지 조사하여 pot실험을 수행하였다. 감자 소괴경의 휴면과 생육에 대한 저선량 ${\gamma}$선 효과는 품종과 저장기간에 따라 다소 차이가 있었다. 대지 품종의 경우 대체로 2,4,8Gy에서 대조구에 비해 높은 맹아 출현율을 보였고, 온실 재배에서는 4,8,16Gy에서 비교적 양호한 생육과 수량 증가 효과를 보였다. 수미 품종에서의 맹아 출현은 2Gy와 4Gy에서 촉진되었고 0.5Gy와 4Gy에서 양호한 생육 및 수량을 보였다. 엽록소 함량은 저선량 ${\gamma}$선 조사구에서 대조구에 비해 뚜렷한 증가효과를 보이지 않았다. 이에 저선량 ${\gamma}$선이 감자의 휴면타파와 생육촉진에 효과가 있음을 확인할 수가 있었다. To observe the stimulating effect of low dose ${\gamma}$ radiation on the dormancy breaking and growth, microtubers induced in vitro of two potato (Solanum tuberosum L. cv. Dejima and cv. Superior) cultivars with different storage duration were irradiated with ${\gamma}$ radiation at the dose of $0.5{\sim}30$ Gy. Sprouting rate, growth and tuber yield of ‘Dejima’ microtuber were increased by ${\gamma}$ radiation in the range of $2{\sim}16$ Gy. In the microtuber of ‘Superior’, the sprouting rate was promoted by 2 Gy and 4 Gy irradiation, and the growth and tuber yield by 0.5 Gy and 4 Gy irradiation. There were not that much difference in chlorophyll content of potato plantlet by the low dose ${\gamma}$ irradiation. These results suggested that low dose ${\gamma}$ radiation stimulated the dormancy breaking and potato growth.

저선량 $\gamma$선 조사가 토마토의 초기생육과 후속고선량 $\gamma$선 저항성에 미치는 영향

김재성,김진규,백명화,김동희,Kim, Jae-Sung,Kim, Jin-Kyu,Back, Myung-Hwa,Kim, Dong-Hee 대한방사선방어학회 1999 방사선방어학회지 Vol.24 No.3

저선량 $\gamma$선 조사한 토마토 2품종, 서광과 하우스모모타로 종자의 발아와 초기생육 및 후속 고선량에 대한 생육효과를 조사하였다. 저선량에 의한 발아와 생육촉진효과는 품종에 따라 달랐다. 발아율의 경우 서광은 모든 저선량 조사구에서 증가되었으나, 하우스모모타로는 오히려 감소되었다. 유묘초장은 저선량 조사에 의해 증가되었으며 두 품종 모두 4 Gy와 8 Gy 조사구에서 가장 효과적이었다. 생육 2개월 후 서광의 초장은 억제되었으나 생체중은 4 Gy와 2 Gy에서 증가되었고, 하우스모모타로의 초장은 12 Gy와 20 Gy에서, 생체중은 20 Gy와 4 Gy에서 가장 높은 증가효과를 보였다. 토마토 식물체의 고선량 피폭에 의한 생육장해는 사전 저선량 조사에 의해 감소되었는데 서광의 경우 2 Gy와 8 Gy 조사구에서 저항성이 높았으며 하우스모모타로의 경우 모든 저선량 조사구에서 저항성 효과가 나타났으며 특히 2 Gy와 12 Gy 조사구에서 높았다. Tomato (Lycopericum esculentum $M_{ILL}$ cv. Seokwang and cv. Housemomotaro) seeds were irradiated with the doses of $1{\sim}20$ Gy from $^{60}Co$ $\gamma$-ray source to investigate the effect of the low dose $\gamma$-ray radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate of seeds irradiated with low dose $\gamma$-ray was enhanced in Seokwang cultivar but not in Housemomotaro cultivar. Seedling height increased in 4 Gy and 8 Gy irradiation group of both cultivars. Plant height of Seokwang cultivar was depressed in low dose irradiation group but fresh weight was increased in 2 Gy and 4 Gy irradiation group. In Housemomotaro cultivar, plant height increased in 12 Gy and 20 Gy irradiation group and fresh weight increased in 4 Gy and 20 Gy irradiation group. Growth inhibition of tomato plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation. Resistance to subsequent high dose of radiation was enhanced in 2 Gy and 8 Gy Irradiation group of Seokwang cultivar and in 2 Gy and 12 Gy irradiation group of Housemomotaro cultivar.

저선량 ${\gamma}$선 조사가 대두 식물체의 방사선 감수성에 미치는 영향

김재성,채성기,백명화,김동희,Kim, Jae-Sung,Chae, Sung-Ki,Back, Myung-Hwa,Kim, Dong-Hee 한국환경농학회 2000 한국환경농학회지 Vol.19 No.4

저선량 조사한 대두종자의 초기생육과 대두 유식물체의 후속고선량에 대한 방사선 감수성 변화를 알아보고자 저선량 0, 4, 8, 12, 20 Gy를 조사하여 재배한 대두 유식물체에 ${\gamma}$선 50, 100, 200 Gy를 조사한 후 생육상황을 관찰하였다. 저선량 조사에 의해 대두 신규종자의 발아율 증가효과는 없었으나 작물 생장 증대효과는 있었으며 저선량 8 Gy 조사구가 가장 효과적이었다. 대두유식물체의 고선량 방사선에 의한 생육장해는 사전 저선량조사에 의해 크게 감소되었으며 저선량 조사에 의한 고선량 방사선 저항성 증가는 8 Gy와 20 Gy조사에서 가장 효과적이었다. Soybean (Glycine max L. cv. Hwangkeum) seeds were irradiated with the dose of $0{\sim}20$ Gy to investigate the effect of the low dose ${\gamma}-ray$ radiation on the early growth and resistance to subsequent high dose of radiation. Germination rate was not enhanced in the seeds irradiated with low dos ${\gamma}-ray$ but plant height and fresh weight increased in the low dose irradiation group. The optimal radiation dose for the growth increasing was 8 Gy in soybean plant. Growth inhibition of soybean plants by high dose radiation was noticeably reduced by pre-irradiation of low dose radiation, Resistance to subsequent high dose of radiation was effective in 8 Gy and 20 Gy irradiation group.

묵은 채소 종자의 발아와 생육 및 효소활성에 미치는 γ 선의 영향

김재성(Jae Sung Kim),백명화(Myung Hwa Back),김동희(Dong Hee Kim),이영근(Young Keun Lee),정규희(Kyu Hoi Chung) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.3

N/A To determine the effect of low dose gamma radiation on the germination and enzyme activities, seeds of Chinese cabbage(Brassica campestris L. cv. Hanyoreum) and radish(Raphanus sativus L. cv. Chungsukoungzoung) were irradiated at the dose of 2∼50㏉. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage. The germination rate of Chinese cabbage was high at 2㏉ and 8㏉ irradiation group and that of radish was high at 2㏉, 6㏉ and 10㏉ irradiation group. Growth of both seedlings of Chinese cabbage and radish increased positively in low dose irradiation group. The height of Chinese cabbage was noticeably high at 4㏉ and 10㏉ irradiation group and that of radish at 6㏉ irradiation group. The protein contents of seedlings from seeds irradiated with the low dose gamma radiation was higher than the control, especially at the early stage. The enzyme activities of seedlings from seeds irradiated with the low dose gamma radiation was high at 4㏉ and 10㏉ irradiation group. These results suggest that the germination, growth and enzyme activities of old vegetable seeds could be promoted by the low dose gamma radiation.

저선량 γ-ray 조사에 의한 고추와 토마토의 생육촉진 효과

이은경(Lee Eun-Kyung),백명화(Back Myung-Hwa),황혜연(Hwang Hye-Yeon),김동희(Kim Dong-Hee),김재성(Kim Jae-Sung),이영복(Lee Young-Bok) 한국원예학회 1998 원예과학기술지 Vol.16 No.1

저선량 방사선이 고추의 초기생육과 후속고선량 내성에 미치는 영향

김재성(Jae Sung Kim),백명화(Myung Hwa Back),김동희(Dong Hee Kim),이영근(Young Keun Lee),이영복(Young Bok Lee) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.1

N/A Pepper(Capsicum annuum L. cv. Jokwang and cv. Johong) seeds were irradiated with the doses of 0∼50㏉ to investigate the effect of the low dose γ-radiation on the early growth and resistance to subsequent high dose of radiation. Following effect was observed in Johong cultivar but not in Jokwang cultivar. Germination and early growth rate of Johong cultivar were noticeably increased at 4, 8 and 20㏉ irradiation group. Resistance to subsequent high dose of radiation of Johong cultivar increased at almost all of the low dose irradiation group. Especially it was highest at 4㏉ irradiation group. Plant groups of Johong cultivar showing the resistance to subsequent high dose of radiation, which had been pre-irradiated with 4㏉ and 8㏉, were also higher in the carotenoid contents and enzyme activity than the plant group not irradiated previously.

저선량 방사선이 저온 저장한 감자 기내 소괴경의 휴면타파와 생육에 미치는 효과

김재성(Jae-Sung Kim),김동희(Dong-Hee Kim),백명화(Myung-Hwa Back),전재흥(Jae-Heung Jeon),이영복(Young-Bok Lee) 한국원예학회 2001 원예과학기술지 Vol.19 No.4

지선량 γ 선이 기내 생산된 감자 소괴경의 휴면과 생에 미치는 효과

김재성(Jae Sung Kim),김동희(Dong Hee Kim),백명화(Myung Hwa Back),전재흥(Jae Heung Jeon) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4

N/A To observe the stimulating effect of low dose γ radiation on the dormancy breaking and growth, microtubers induced in vitro of two potato(Solanum tuberosum L. cv. Dejima and cv. Superior) cultivars with different storage duration were irradiated with γ radiation at the dose of 0.5∼30㏉. Sprouting rate, growth and tuber yield of Dejima microtuber were increased by γ radiation in the range of 2∼16㏉. In the microtuber of Superios, the sprouting rate was promoted by 2㏉ and 4㏉ irradiation, and the growth and tuber yield by 0.5㏉ and 4㏉ irradiation. There were not that much difference in chlorophyll content of potato plantlet by the low dose γ irradiation. These results suggested that low dose γ radiation stimulated the dormancy braking and potato growth.

묵은 고추종자의 발아와 생리활성에 미치는 저선량 방사선조사 효과

김재성,백명화,이혜연,이영근 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.4

저선량 방사선 조사가 묵은 고추종자의 발아율과 발아종자의 생리활성에 미치는 효과 등을 알아보고자 4년 저장한 조광고추와 3년 저장한 홍광고추 종자에 저선량 r선을 2∼50 Gy 까지 조사하여 관찰한 결과는 다음과 같다. 저선량 조사한 고추종자의 발아율은 대조구보다 증가하는 경향을 보였다. 특히 발아초기에 그 효과가 뚜렷하게 나타나 파종 7일 후에 조광고추 품종은 4 Gy 조사구에서 74%, 홍광고추 품종은 8 Gy 조사구에서 11% 증가하였다. 고추의 유묘초장에서도 거의 모든 저선량 조사구에서 증가하였는데 특히 조광고추 품종은 4 Gy 조사구에서, 홍광고추 품종은 8 Gy 조사구에서 유의성있는 증가효과를 보였다. 저선량 조사한 종자의 단백질 함량은 조광 고추 품종은 발아후기에, 홍광고추 품종은 발아초기에 증가하였고, catalase와 peroxidase 활성은조광고추 품종은 4 Gy 조사구에서, 홍광고추 품종은 8 Gy 조사구에서 높은 값을 보였다. 이에 저선량 r선 조사에 의해 묵은 종자의 발아와 생리활성이 촉진됨을 확인 할 수 있었다. To observe the stimulating effects of low dose gamma radiation on the germination and physiological activity of germinating seeks of old red pepper (Capsicum annuum L. cv. Jokwang and cv. Hongkwang), seeds were irradiated at the dose of 2∼50 Gy. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage of induction. The germination rate at 7 days after sowing in Jokwang and Hongkwang cultivar was high as 74% and 11% at 4 Gy and 8 Gy irradiation group, respectively. The seedling height of Jokwang cultivar was noticeably high at 4 Gy irradiation group and that of Hongkwang cultivar at 8 Gy irradiation group. The protein contents of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at the late stage of induction and that of Hongkwang cultivar at the early stage of induction. Catalase and peroxidase activities of seedlings from seeds irradiated with low dose gamma radiation of Jokwang cultivar increased at 4 Gy irradiation group and that of hongkwang cultivar at 8 Gy irradiation group.