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배우철,정욱진,정병철,장승욱 明知大學校 自然科學硏究所 1997 자연과학논문집 Vol.15 No.-
오염하천인 중랑천에서 중금속 내성세균 J7을 분리하여 생육과 카드뮴 흡착특성을 조사하였다. 회분배양에 미치는 카드뮴의 영향을 조사한 결과 카드뮴을 첨가하지 않았을 경우에는 비생장속도 0.32hr¹유도기 1시간, 카드뮴 106ppm의 경우에는 0..6hr¹, 12시간, 카드뮴 265ppm의 경우에는 0.04hr¹, 24시간으로 카드뮴 농도 증가에 따라 비생장속도의 감소와 유도기의 증가를 나타냈다. 카드뮴 제거량은 106.2㎎/g DCW, 191.6㎎/g으로 카드뮴 농도의 증가에 따라 높게 나타났다. 최적 카드뮴 흡착 pH는 8.0으로 이때 78㎎/g/ DCW의 효율을 나타냈다. 이처럼 생장시의 제거량이 생장후의 단순흡착량보다 더 높게 나타나는 것으로 미루어 생장시에는 카드뮴 첨가에 의한 유도과정에 의해 단순흡착량보다 더 높게 나타나는 것으로 미루어 생장시에는 카드뮴 첨가에 의한 유도과정에 의해 단순흡착외의 다른 중금속 제거기작도 작용하는 것으로 여겨진다. J7에 의한 카드뮴 흡착은 Langmuir 흡착등온식에 적합하였고 Scatchard Plot에서 직선을 나타낸 것으로 보아 단층 흡착이고 카드뮴의 농도 변화에 흡착능이 큰 영향을 받지않는 안정된 상태를 갖는 것으로 보인다. 낮은 농도에서 흡착제의 효율을 확인하는 Freundlich 흡착등온식에서 흡착강도 (1/n)이 0.3178로 흡착제로의 사용가능성이 높고 흡착능(K) 은 1.331로 높게 나타났다. Cadmium tolerant bacterium J7 was isolated from Jungrang stream and investigated in terms of growth and cadmium adsorption properties. The specific growth rate of the bacterium was 0.32hr¹at Oppm of cadmium concentration, and the lag phase continued for lhour. But when the cadmium concentration was 106ppm. the specific growth rate and the duration of lag phase were 0.06hr¹ and 12hour, respectively. When initial cadmium concentration was 265ppm, they were 0.04hr¹ and 24hour. This result showed that the specific growth rate decreased but the lag phase increased as the cadmium concentration increased. The quantities of cadmium removed by J7 was 106.2㎎ cadmium/ g dry cell weight at 106ppm and 191.6㎎/g at 265ppm of cadmium. The maximum cadmium adsorption of pre-grown cell was 78 ㎎/g at pH 8.0 The removal capacitiy of growing cell was higher than adsorption capacites of resting cell after growth, and this indicates the addition of cadmium induced another metal -removing mechanism of the cell. Langmuir isotherm and scatchard plot were suitable models to explain the cadmium removal by J7. This indicates that single layer adsorption and the change of cadmium concentration has little effect on adsorption capacity of the bacterium, The Frendlich isotherms was available to measure the adsorption efficiency at low metal concentration. Adsorption strength(1/n) was 0.3178 and capacity(K) was 1.331. This indicates the possiblity of the application of the bacterium as an adsorbate at low metal concentration.
영지버섯 균사체(Ganoderma lucidum IY009)로부터 추출한 $\beta$-Immunan의 시스플라틴 유발 신독성 보호효과
김용석,배우철,박정민,이준우,백성진,이상봉,윤경하 한국미생물 · 생명공학회 2004 한국미생물·생명공학회지 Vol.32 No.3
$\beta$-Immunan was proteoglycan obtained from mycelium of Ganoderma lucidum IY009. In this study, the protective effects of $\beta$-Immunan, against the CDDP induced in vitro cytotoxicity and in vivo renal toxicity, was measured. Concentration dependent cytotoxicities of CDDP in normal kidney cells (Vero, TCMK-l) were reduced by $\beta$-Immunan treatment. Increased renal toxicity factors, such as elevation of blood urea nitrogen (BUN) and serum creatinine, reduction of kidney weight and malonidialdehyde (MDA), by intraperitoneal administration of CDDP in rats was improved. These results indicated that $\beta$-Immunan have a protective effects against the CDDP induced renal toxicity, however, it needed to confirm the detailed mechanism for therapeutic effects.
Zoogloea ramigera 115와 Zoogloea ramigera l15SLR의 중금속 흡착능 비교
이한기,배우철,진욱,정욱진,이삼빈,정병철,Lee, Han-Ki,Bae, Woo-Chul,Jin, Wook,Jung, Wook-Jin,Lee, Sam-Pin,Jeong, Byeong-Chul 한국미생물·생명공학회 1998 한국미생물·생명공학회지 Vol.26 No.1
Capsular polymer인 zooglan을 생산하는 Z. ramigera 115와 수용성 slime polymer 생산 변이주인 Z. ramigera l15SLR의 중금속 제거능을 비교하였다. 중금속 5종($Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$, $Fe^{2+}$)의 최저 생장저해농도는 두 균주 모두 유사하였다. $Cd^{2+}$을 첨가하지 않은 경우에 비해 $Cd^{2+}$ 500ppm을 첨가한 액체 배지에서의 생체량은 Z. ramigera 115는 24%, Z. ramigera l15SLR은 50%로 감소하였고 동일한 생체량에 대하여 Z. ramigera 115SLR이 Z. ramtgera 115보다 $Cd^{2+}$을 1.5배 더 제거하였다. 세포와 polymer를 분리하여 중금속 흡착능을 비교한 결과 polymer가 세포보다 높은 흡착량을 나타냈으며 최적 중금속 흡착 pH는 7.5였다. Langmuir 흡착등온선과 Freundlich 흡착등온선을 분석한 결과 $Cd^{2+}$에 대하여 Z. ramigera l15SLR polymer는 Z. ramigera 115 및 이온 교환 수지보다 높은 최대흡착량과 흡착안정성을 보여서 홉착제로서 유용할 것으로 판단된다. Heavy metal removal by Z. ramigera 115 and soluble slime polymer producing mutant Z. ramigera 115SLR was investigated. Both strains showed similar tolerance against $Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$ and $Fe^{2+}$. When cells were cultivated in the presence of 500 ppm $Cd^{2+}$, the mutant strain removed 1.5 fold more metal than the wild type did at same biomass. Metal adsorption capacities were in the order of Z. ramigera l15SLR polymer > Z. ramigera 115 polymer > Z. ramigera 115 cell >Z. ramigera l15SLR cell. The optimum pH for metal adsorption was 7.5. Langmuir and Freundlich isotherms indicated that Qmax and 1/n of Z. ramigera l15SLR polymer were 164.2 mg $Cd^{2+}$/g dw and 0.496, respectively. These results showed that the polymer of Z. ramigera l15SLR could be used as an effective metal adsorbate.
Zoogloea ramigera 115와 Zoogloea ramigera 115SLR의 중금속 흡착능 비교
이한기,배우철,진욱,정욱진,이삼빈,정병철 한국산업미생물학회 1998 한국미생물·생명공학회지 Vol.26 No.1
Capsular polymer인 zooglan을 생산하는 Z. ramigera 115와 수용성 slime polymer 생산 변이주인 Z. ramigera 115SLR의 중금속 제거능을 비교하였다. 중금속 5종(Cd^2+, Co^2+, Cu^2+, Ni^2+, Fe^2+)의 최저 생장저해농도는 두 균주 모두 유사하였다. Cd^2+을 첨가하지 않은 경우에 비해 Cd^2+ 500 ppm을 첨가한 액체 배지에서의 생체량은 Z. ramigera 115는 24%, Z. ramigera 115SLR은 50%로 감소하였고 동일한 생체량에 대하여 Z. ramigera 115SLR이 Z. ramigera 115보다 Cd^2+을 1.5배 더 제거하였다. 세포와 polymer를 분리하여 중금속 흡착능을 비교한 결과 polymer가 세포보다 높은 흡착량을 나타냈으며 최저 중금속 흡착 pH는 7.5였다. Langmuir 흡착등온선과 Freundlich 흡착등온선을 분석한 결과 Cd^2+에 대하여 Z. ramigera 115SLR polymer는 Z. ramigera 115 및 이온 교환 수지보다 높은 최대흡착량과 흡착안정성을 보여서 흡착제로서 유용할 것으로 판단된다. Heavy metal removal by Z. ramigera 115 and soluble slime polymer producing mutant Z. ramigera 115SLR was investigated. Both strains showed similar tolerance against Cd^2+, Co^2+, Cu^2+, Ni^2+ and Fe^2+. When cells were cultivated in the presence of 500 ppm Cd^2+, the mutant strain removed 1.5 fold more metal than the wild type did at same biomass. Metal adsorption capacities were in the order of Z. ramigera 115SLR polymer>Z. ramigera 115 polymer>Z. ramigera 115 cell>Z. ramigera 115SLR cell. The optimum pH for metal adsorption was 7.5. Langmuir and Freundlich isotherms indicated that Qmax and 1/n of Z. ramigera 115SLR polymer were 164.2 ㎎ Cd^2+/g dw and 0.496, respectively. These results showed that the polymer of Z. ramigera 115SLR could be used as an effective metal adsorbate
영지버섯 균사체 ( Ganoderma lucidum IYO09)로부터 추출한 단백다당체의 전이암 억제 효과
백성진(Seong Jin Baek),김용석(Yong Seok Kim),용환미(Hwan Mee Yong),채주병(Ju Byung Chae),이선애(Sun Ae Lee),배우철(Woo Chul Bae),박동우(Dong Woo Park),김동연(Dong Yeon Kim),이준우(June Woo Lee),박승국(Seung Kook Park) 대한약학회 2002 약학회지 Vol.46 No.1
β-Immunan, a proteoglycan, was isolated from the mycelium of Ganoderma lucidum which belongs to a medicinal mushroom. The effects of β-Immunan on cell- cell and cell-matrix interactions mediated by carbohydrate-recognition and the mechanism responsible for the inhibition of experimental metastasis of Bl6-FIO and B16/BL6 murine melanoma were studied. The results showed that Ganoderma lucidum-Immunan inhibited Bl6-Fl melanoma cell's adhesion to laminin and asialofetuin-induced homotypic aggregation and reduced invasion against Bl6-FIO murine melanoma cells through matrigel in vitro assay: When β-Immunan was intraperitoneally administrated to C57BL/6 mice bearing B16/BL6 murine melanoma cells, it was decreased the number of pulmonary metastatic colony by the dose dependent manner ranging from 20 to 100 mg/kg/day, The results indirectly indicate that clinical treatment with β-Immunan might be expected to exhibit anti-metastatic effect. In the put-monary metastasis, the number of pulmonary metastatic colony of melanoma when β-Immunan was intraperitoneally aduunistrated to C57BL/6 mice bearing Bla/BL6 murine melanoma cells by intravenous injection were decreased by the dose dependent manner ranging from 20 to 100 mg/kg/day.