무모생쥐에서 국소 Tacrolimus 연고가 Diphenylcyclopropenone에 의한 접촉과민반응과 반복 도포에 의한 피부염에 미치는 영향

박현제 ( Hyun Je Park ),장호선 ( Ho Sun Jang ),장봉석 ( Bong Seok Jang ),김문범 ( Moon Bum Kim ),권경술 ( Kyung Sool Kwon ),오창근 ( Chang Keun Oh ) 대한피부과학회 2007 대한피부과학회지 Vol.45 No.8

Background: Topical tacrolimus is widely used for the treatment of inflammatory skin diseases like atopic dermatitis, but there are few studies about the effect of topical tacrolimus for allergic contact dermatitis. Allergic contact dermatitis develops in two phases, the clinically silent sensitization phase, and the clinically apparent elicitation phase. Objective: The purpose of this study is to investigate whether topical tacrolimus has an effect on both phases of murine contact hypersensitivity and dermatitis of repeated applications induced by diphenylcyclopropenone (DPCP). Methods: Hairless mice were treated with topical tacrolimus before and after DPCP challenging. The suppressive effect of topical tacrolimus was measured by skin erythema, ear swelling, weight change and cell numbers of local lymph nodes. In addition, a biopsy was carried out and epidermal hyperplasia was investigated microscopically. TNF-α mRNA on the mice which were treated with topical tacrolimus to one side of the ears was measured before and after being chronically challenged with DPCP on both ears. Results: Topical tacrolimus pretreatment dramatically supressed inflammatory reactions in the sensitization phase, and treatment of topical tacrolimus after sensitization dramatically supressed inflammatory reactions in the elicitation phase. Topical tacrolimus also dramatically supressed inflammatory reactions in the repeated DPCP-induced dermatitis. Conclusion: The data revealed topical tacrolimus could effectively suppress murine contact hypersensitivity and dermatitis of repeated applications induced by DPCP. Putting these results together, topical tacrolimus can be very effective in not only the treatment but also the prevention of allergic contact dermatitis. Larger studies are needed to determine the clinical relevance. (Korean J Dermatol 2007;45(8):761∼767)

탄소 및 질소안정동위원소 조성에 의한 남해안 진동만 양식 미더덕의 먹이원 평가

문창호,박현제,윤성규,곽정현,Moon, Changho,Park, Hyun Je,Yun, Sung Gyu,Kwak, Jung Hyun 한국해양학회 2014 바다 Vol.19 No.4

To examine the trophic ecology of the ascidian Styela clava in an aquaculture system of Korea, stable carbon and nitrogen isotopes were analyzed monthly in S. clava, coarse ($>20{\mu}m$, CPOM) and fine particulate organic matters ($0.7<<20{\mu}m$, FPOM). CPOM (means: $-18.5{\pm}1.2$‰, $9.3{\pm}0.7$‰) were significantly higher ${\delta}^{13}C$ and ${\delta}^{15}N$ values than those ($-20.5{\pm}1.5$‰, $8.4{\pm}0.5$‰) of FPOM. S. clava had mean ${\delta}^{13}C$ and ${\delta}^{15}N$ values of $-18.9({\pm}1.7)$‰ and $11.6({\pm}0.7)$‰, respectively. S. clava were more similar to seasonal variations in ${\delta}^{13}C$ and ${\delta}^{15}N$ values of FPOM than those of CPOM, suggesting that they rely largely on the FPOM as a dietary source. In addition, our results displayed that the relative importance between CPOM and FPOM as dietary source for the ascidians can be changed according to the availability of each component in ambient environment, probably reflecting their feeding plasticity due to non-selective feeding irrespective of particle size. Finally, our results suggest that dynamics of pico- and nano-size plankton (i.e., FPOM) as an available nutritional source to S. clava should be effectively assessed to maintain and manage their sustainable aquaculture production. 2008년 4월에서 2009년 1월 사이에 진동만 미더덕의 섭식 생태를 설명하기 위하여 $20{\mu}m$ 이상(coarse particulate organic matter, CPOM) 그리고 $0.7{\mu}m$ 이상 $20{\mu}m$ 이하(fine POM)의 크기가 나누어진 각각의 부유입자유기물과 미더덕에 대한 ${\delta}^{13}C$ 과 ${\delta}^{15}N$ 값의 월별 변동을 비교 분석하였다. CPOM과 FPOM의 ${\delta}^{13}C$ 및 ${\delta}^{15}N$을 월별로 비교한 결과 전체적으로 CPOM(평균 $-18.5{\pm}1.2$‰, $9.3{\pm}0.7$‰)이 FPOM(평균 $-20.5{\pm}1.5$‰, $8.4{\pm}0.5$‰)에 비해 유의하게 높은 값을 나타내었다. 미더덕의 ${\delta}^{13}C$과 ${\delta}^{15}N$ 값은 각각 평균 $-18.9({\pm}1.7)$‰과 $11.6({\pm}0.7)$‰로 나타났는데, 이와 같은 비값들은 CPOM 보다는 FPOM의 월별 변동과 비슷한 경향을 보여 이들이 CPOM에 비하여 상대적으로 FPOM에 더 높은 영양 의존도를 가진다는 것을 강하게 시사해 주었다. 그러나 미더덕의 먹이원으로서 CPOM과 FPOM의 상대적인 중요성은 시기에 따른 각 요소의 가용성(availability)에 크게 의존하는 것으로 나타났는데, 이와 같은 결과는 부유입자물질의 크기에 관계없이 미더덕에 의한 비선택적 먹이 섭식을 잘 반영하는 듯 하였다. 본 연구 결과는 미더덕의 지속적인 양식생산을 유지하기 위해 가용 먹이원으로서 식물플랑크톤과 함께 미소 부유입자유기물의 유용성을 고려해야함을 잘 나타내 주었다.

모즈 미세도식 수술의 수술 시간에 대한 분석: 단일 기관의 경험

문제호 ( Je Ho Mun ),박현제 ( Hyun Je Park ),김수한 ( Su Han Kim ),정도상 ( Do Sang Jung ),고현창 ( Hyun Chang Ko ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ),김훈수 ( Hoon Soo Kim ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.7

Background: Mohs micrographic surgery (MMS) is a precise method of treating skin cancer. There have been many studies about the advantages and disadvantages of MMS. However, no study has yet been carried out regarding the length of surgery. Objective: The purpose of this study was to report our experience with MMS for the treatment of skin cancers and to analyze the operative time of MMS. Methods: We analyzed 50 cases of skin cancers treated by MMS at the Dermatosurgery Clinic in Pusan National University Hospital between April 2009 and November 2009. Results: The minimum and maximum operative times of MMS were 88 and 356 minutes, respectively, and the mean operative time was 171.4 minutes. The mean number of stages was 1.7 (range, 1~4). The mean operative time per stage was 93.9, 62.9, 57.2, and 53 minutes for the 1st, 2nd, 3rd, and 4th stages, respectively. The time percentages for a pathologic consult to the Department of Pathology per stage was 42%, 50%, 52%, and 57% for the 1st, 2nd, 3rd, and 4th stages, respectively; therefore, the consultation to the Department of Pathology occupied a high percentage of the operative time. The mean operative time of repair for surgical defects was 44.4 minutes. Limitations: The results of this study are based on the experience of a single surgical team in a single institution. Conclusion: The results of our study reveal that MMS is a time-consuming operation. Further studies are required to shorten the operation time of MMS.

강릉 남대천 부유입자유기물의 탄소안정동위원소 비와 C/N 비의 시·공간 변동

곽정현,박현제,Kwak, Jung Hyun,Park, Hyun Je 한국환경과학회 2020 한국환경과학회지 Vol.29 No.5

To understand the composition, quantity, and quality of Suspended Particulate Organic Matter (SPOM) in the Gangneung Namdae Stream, Korea, we examined spatiotemporal variations in water temperature, salinity, chlorophlly a (Chl a), Particulate Organic Carbon (POC) and nitrogen (PON), and carbon stable isotope (δ¹³C) of SPOM at six stations in June (pre-monsoon), July (monsoon), and September (post-monsoon) 2017. With increasing precipitation, the average POC and C/N values increased significantly in July than in June. In September, the values decreased with decreasing precipitation. The δ¹³C values showed irregular spatiotemporal fluctuations among the stations and periods, thereby suggesting a greater contribution of autochthonous organic matter to the pool of SPOM than that of allochthonous organic matter derived from upstream. In addition, the large and irregular changes in POC, C/N ratio, C:Chl a, and δ¹³C compared to that of PON were observed for all periods among the stations, indicating a serial discontinuity of the stream. Our results suggest that the Gangneung Namdae Stream is significantly influenced by the increase in freshwater discharge caused by heavy rainfalls during the summer monsoon and post-monsoon periods.

${\delta}^{13}C$ 분석에 의한 남해 연안 내만역 어류 영양원으로써 저서생산의 중요성 평가

강창근,최은정,김영상,박현제,Kang, Chang-Keun,Choy, Eun-Jung,Kim, Young-Sang,Park, Hyun-Je 한국해양학회 2009 바다 Vol.14 No.1

남해 연안의 3개 내만역을 대상으로 전체 어류군집이 이용하는 유기물의 기원을 조사하기 위하여 잘피와 부착조류, 저서미세조류, 해조류, 습지식물, 부유입자유기물(식물플랑크톤), 습지식물(갈대) 그리고 육상기원 유기물 등 다양한 유래의 유기물에 대한 탄소안정동위원소 비(${\delta}^{13}C$) 값과 어류 체조직의 ${\delta}^{13}C$ 값을 비교 분석하였다. 연구해역에서 채집된 총 31종의 어류들이 가지는 ${\delta}^{13}C$C 값은 -16.2${\sim}$-8.3‰의 범위를 나타내어 잘피(-8.3${\pm}$1.9‰), 부착조류(-12.4 ${\pm}$0.6‰), 저서미세조류(-15.4${\pm}$1.6‰) 그리고 해조류(-16.0${\pm}$1.8‰)가 가지는 ${\delta}^{13}C$ 값에 상당하는 범위를 나타내었다. 또한 연구수역의 어류군집이 가지는 ${\delta}^{13}C$ 값(-12.9${\pm}$1.5‰)은 남해 외양역에서 채집된 어류(-17.3${\pm}$0.8‰)와 낙동강 수계에서 채집된 어류군집(-23.2${\pm}$1.6‰)이 가지는 값에 비하여 뚜렷히 높은 값을 보였다. 따라서 본 조사에서 얻어진 ${\delta}^{13}C$ 분석 결과들은 조사가 수행된 3개 내만역 어류군집은 만 자체의 자생기원 저서생산에서 유래한 탄소원에 주로 의존하고 있다는 것을 시사하였다. Stable carbon isotope analysis was used to investigate the autotrophic carbon sources supporting fisheries in three coastal bay systems of the southern coast of Korea. Carbon isotope ratios (${\delta}^{13}C$) of 31 fish species were analysed and compared with those of a range of potential primary food sources [e.g., seagrass (Zostera marina), seagrass epiphytes, benthic microalgae, macroalgae, marine particulate organic matter (marine POM), marsh plant (Phragmites australis) and terrestrial POM]. ${\delta}^{13}C$ values (range, -16.2${\sim}$-8.3‰) of fishes from the coastal embayment systems were overlapped with those of seagrass (-8.3${\pm}$1.9‰), seagrass epiphytes (-12.4${\pm}$0.6‰), benthic microalgae (-15.4${\pm}$1.6‰) and macroalgae (-16.0${\pm}$1.8‰). In addition, fishes (-12.9${\pm}$1.5‰) from the study area had distinctly higher ${\delta}^{13}C$ values compared to those collected in offshore sites (-17.3${\pm}$0.8‰) of the southern sea of Korea and Nakdong River (-23.2${\pm}$1.6‰). This result indicates that carbon supporting fish communities of these coastal bay systems is mainly derived from the local benthic producers.

동위원소 분석에 의한 동해와 남해 연안의 상이한 해조류 군락에 서식하는 저서무척추동물 먹이원 평가

강창근,최은정,송행섭,박현제,서인수,조규태,이근섭,Kang, Chang-Keun,Choy, Eun-Jung,Song, Haeng-Seop,Park, Hyun-Je,Soe, In-Soo,Jo, Q-Tae,Lee, Kun-Seop 한국해양학회 2007 바다 Vol.12 No.4

암반 해안의 해조 군락에 서식하는 저서무척추동물들에 의해서 이용되는 유기물의 기원을 밝히기 위하여 저서동물과 그들의 잠재 먹이원에 대한 탄소와 질소 안정동위원소 비($\delta^{13}C$ 및 $\delta^{15}N$)값 조성을 분석하였다. 조사는 부영양화된 반폐쇄성 내만인 남해안의 광양만 연안 암반 조간대와 상대적으로 빈영양의 외양에 노출된 환경 특성을 가지는 동해안의 대진(삼척)연안의 암반 조하대를 대상으로 2005년 5월부터 6월 사이에 실시되었다. 암반에서 노출되어 서식하는 부유물 섭식자들은 다른 섭식 양식을 가지는 동물군에 비해 상대적으로 낮은 $\delta^{13}C$ 값을 가져 이들이 식물플랑크톤에서 유래하는 유기물을 주로 이용하여 표영먹이망과 강한 영양연결을 가지는 것으로 나타났다. 반면, 암반틈 속에서 서식하는 일부 부유물 섭식자를 포함한 초식동물과 퇴적물 섭식자 및 육식성 포식자들은 상대적으로 높은 $\delta^{13}C$ 값을 가져 해조류에서 유래한 유기물을 주로 이용하여 저서먹이망을 형성하였다. 따라서 부유물 섭식자를 제외하고 해조 군락을 서식처로 하는 대부분의 동물들이 해조류에서 유래한 유기물을 이용하는 저서먹이망으로 강한 영양연결을 가져 연안 암반 생태계에서 생물생산과 생물 다양성의 유지에 대한 해조류의 중요성을 잘 나타내었다. 또한 광양만과 같은 부영양화된 연안에서 상대적으로 빈영양인 동해 연안 조하대의 저서동물과 해조류에 비하여 높은 $\delta^{15}N$ 값 분포는 육상으로부터 유입되는 영양염이 암반 조간대의 해조류 생산에 이용되고 이어서 이 특정 연안 생태계의 먹이망으로 편입되는 경로를 가지는 것을 시사하였다.축과 높은 정밀도 및 정확도로 반복적인 유기탄소분석에 유용할 것으로 사료된다.개월의 편차를 보였다. 이처럼 연령을 모르는 우리나라의 혼합치 열기 아동에서 파노라마 방사선 사진을 이용하여 연령 추정시 위의 방정식을 사용한다면 비교적 정확하게 연령을 추정할 수 있으리라고 사료된다. 상아질 접착제의 종류는 별다른 영향을 주지 않은 것으로 분석되었다. 결합강도의 비교에서 컴포머가 글라스 아이어노머에 비해 우수한 것으로 평가되어 소아 환자의 유구치 심미 수복재료로서의 가능성이 입증되었다.후 중합한 군이 산소억제층의 두께가 평균 49%의 감소되었으며(p<0.05), 이들 산소를 차단한 군 간의 유의차는 없었다.며 CYP1A2유전자형에 따른 영향은 관찰할 수 없었다. CYP1A2유전자형에 따른 생체내 대사능을 관찰하는 실험이 향후 이루어 져야 할 것으로 사료된다.san film보다 큰 수증기 투과도를 보였다.적으로 유의한 차이를 보이지 않았다.y tissue layer thinning은 3 군모두에서 관찰되었고 항암 3 일군이 가장 심하게 나타났다. 이상의 실험결과를 보면 술전 항암제투여가 초기에 시행한 경우에는 조직의 치유에 초기 5 일정도까지는 영향을 미치나 7 일이 지나면 정상범주로 회복함을 알수 있었고 실험결과 항암제 투여후 3 일째 피판 형성한 군에서 피판치유가 늦어진 것으로 관찰되어 인체에서 항암 투여후 수술시기는 인체면역계가 회복하는 시기를 3주이상 경과후 적어도 4주째 수술시기를 정하는 것이 유리하리라 생각되었다.한 복합레진은 개발의 초기단계이며, 물성의 증가를 위한 연구가 필요할 것으로 사료된다.또 다른 약물인 glycyrrhetinic acid($100{\mu}M$)도 CCh 자극으로 Stable carbon and nitrogen isotopes were analyzed in suspended particulate organic matter, macroalgae and macrobenthic invertebrates in order to determine the importance of primary organic matter sources in supporting food webs of rocky subtidal and intertidal macroalgal beds in the Korean coasts. Investigations were conducted at the inter tidal sites within Gwangyang bay, a semi-enclosed and eutrophicated bay, and the subtidal sites of the east coast, a relatively oligotrophic and open environment, in May and June 2005. Water-column suspension feeders showed more negative $\delta^{13}C$ values than those of the other feeding guilds, indicating trophic linkage with phytoplankton and thereby association with pelagic food chains. In contrast, animals of the other feeding guilds, including interface suspension feeders, herbivores, deposit feeders, omnivores and predators, displayed relatively less negative $\delta^{13}C$ values than those of the water-column suspension feeders and similar with that of macroalgae, indicating exclusive use of macroalgae-derived organic matter and association with benthic food chains. Most the macrobenthic species were considered to form strong trophic links with benthic food chains. In addition, the distribution of higher $\delta^{15}N$ values in macrobenthic consumers and macroalgae at the intertidal sites of Gwangyang Bay than those at the subtidal sites of the east coast suggests that anthropogenic nutrients may enhance the macroalgal production at the intertidal sites and in turn be incorporated into the particular littoral food web in Gwangyag Bay. These results confirm the dominant role of macroalgae in supporting rocky subtidal and intertidal food webs in the Korean coasts.

위내용물 분석과 안정동위원소 분석을 이용한 겨울철 동해 북부 연안에 출현하는 명태 (Gadus chalcogrammus)와 대구 (G. macrocephalus)의 먹이분할 연구

박주면 ( Joo Myun Park ),정해근 ( Hae Kun Jung ),이충일 ( Chung Il Lee ),박현제 ( Hyun Je Park ) 한국어류학회 2022 韓國魚類學會誌 Vol.34 No.2

본 연구는 위내용물 분석과 안정동위원소 (δ¹³C and δ¹⁵N) 분석을 통하여 우리나라 동해 북부 연안에 출현하는 명태 (Gadus chalcogrammus)와 대구 (G. macrocephalus)의 종내 및 종간 먹이자원 분할을 조사하였다. 두 종은 중층성 육식성 어종으로 명태는 저서성 및 중층성 갑각류를 주로 섭식하였고, 대구는 어류를 주로 섭식하였다. 위내용물 분석 결과에 대한 Non-metric multidimensional scaling (nMDS) ordination과 permutational multivariate analysis of variance(PERMANOVA)은 두 종의 종내 및 종간 먹이조성의 차이와 먹이자원 분할을 보여줬다. 안정동위원소 분석 결과 δ¹⁵N 값은 종간 유사하였지만, δ¹³C 값은 대구가 높은 값을 나타내어 두 종간 생태지위 차이를 보여줬다. 명태는 체장 증가에 따라 큰 체장군에서 새우류와 두족류를 더 많이 섭식하는 먹이 전환을 나타냈지만, 대구는 체장군 간 먹이조성이 유사하였다. 안정동위원소 분석에서도 체장군 간 차이를 보였는데, 두 종의 큰 체장군은 작은 체장군에 비해 더 높은 δ¹⁵N 값을 나타낸다. 결론적으로 본 연구는 명태와 대구의 위내용물 분석과 안정동위원소 분석을 통하여 종내 및 종간 먹이 차이와 생태지위 분할의 증거를 보여줬다. This study investigated dietary habits and intra- and inter-specific food resource partitioning of co-occurring walleye pollock (Gadus chalcogrammus) and Pacific cod (G. macrocephalus) from the waters off the north-eastern coast of South Korea using stomach contents and stable isotopes (δ¹³C and δ¹⁵N) analyses. Both species are mesopelagic carnivores that consumed mainly benthopelagic crustaceans, but teleosts were also abundant in the diet of Pacific cod. Non-metric multidimensional scaling (nMDS) ordination and permutational multivariate analysis of variance (PERMANOVA) of dietary data revealed significant intra- and inter-specific dietary differences, i.e., food resource partitioning. Nitrogen stable isotope values (δ¹⁵N) were similar between walleye pollock and Pacific cod, but carbon stable isotope values (δ¹³C) were significant different, suggesting different trophic positioning. Canonical analysis of principal coordinate (CAP) ordination plot further demonstrated that differences in the type and range of prey ingested by the two species contributed such an inter-specific difference in the diet compositions. Ontogenetic changes in diet compositions were evident. As walleye pollock, they preyed more upon carid shrimps and cephalopods, but no such trend was observed in the diets of Pacific cod. While stable isotope values indicated that large-sized specimens of both species were significantly enriched in 15N relative to smaller conspecifics thus supporting these data. Consequently, in this study, both methodologies, i.e., stomach contents and stable isotope analyses, provided evidence of inter- and/or intra-specific dietary segregations and trophic niche partitioning between co-occurring walleye pollock and Pacific cod off eastern Korean waters.

등에 발생한 원발성 피부 상피양 혈관내피세포종

김원정 ( Won Jeong Kim ),박현제 ( Hyun Je Park ),김훈수 ( Hoon Soo Kim ),진현우 ( Hyun Woo Chin ),김수한 ( Su Han Kim ),고현창 ( Hyun Chang Ko ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.3

Epithelioid hemangioendothelioma (EHE) is an uncommon vascular neoplasm that is considered to be a low-grade malignancy with a severity between an angiolymphoid hyperplasia with eosinophilia and an epithelioid hemangiosarcoma. The appearance of cutaneous EHE is extremely rare and only about 20 cases have been reported in the Korean and English literatures. A 70-year-old man presented with an approximately 1-year history of an isolated painful subcutaneous nodule of the back. A histopathologic examination revealed nests and cords of epithelioid eosinophilic cells in hyalinized stroma with intracytoplasmic vacuoles. Tumor cells were strongly positive for the endothelial cell markers CD31 and CD34, and we didn`t find any metastatic lesions under radiologic examination. On the basis of these findings, the diagnosis of primary cutaneous EHE was made. To our knowledge, cutaneous EHEs were reported in only 2 cases in the Korean literature, and that of the trunk has not been published previously. Moreover, there is no meta-analytic study about cutaneous EHE. So, we report here a rare case of EHE of the back and review the relevant literature. (Korean J Dermatol 2011;49(3):269∼273)

표재성 진균증에서 Human β Defensin-2 발현에 관한 면역조직화학적 연구

권경술 ( Kyung Sool Kwon ),박현제 ( Hyun Je Park ),장봉석 ( Bong Seok Jang ),김문범 ( Moon Bum Kim ),오창근 ( Chang Keun Oh ),장호선 ( Ho Sun Jang ) 대한피부과학회 2006 대한피부과학회지 Vol.44 No.5

Background: Normal human skin is resistant to infection with various kinds of microorganisms by producing anti-microbial chemicals. Human β defensin-2 (hBD-2) is an anti-microbial peptide that has recently been shown to be expressed in various epithelial cells and inflammatory diseases. However, the expression of hBD-2 in fungus- infected skin is not well-known. Objective: This study was performed to investigate the expression pattern of hBD-2 in superficial mycosis. Methods: Using the immunohistochemical method with formalin-fixed, paraffin-embedded sections, we checked the expression levels and localization of hBD-2 in lesional skin samples of tinea capitis (5 patients), tinea corporis (6 patients), candidiasis (3 patients), Malassezia folliculitis (2 patients), and psoriasis (3 patients) as positive control, and normal skin samples from 6 healthy subjects as negative control. Results: The expression of hBD-2 was not observed in normal skin, but moderate to strong expression of hBD-2 was observed in the epidermis, and the papillary dermal infiltrating cells of psoriasis. In tinea capitis, strong hBD-2 expression was found in the upper spinous layer of epidermis and follicular epidermis, and perifollicular inflammatory cells. In tinea corporis and candidiasis, mild to strong expression of hBD-2 was found in the horny or spinous layer of epidermis and infiltrating inflammatory cells. Strong hBD-2 expression was found in the follicular epidermis and perifollicular inflammatory cells of Malassezia folliculitis. Conclusion: These results suggest that hBD-2 plays an important role in cutaneous innate immune defense against fungal infection. (Korean J Dermatol 2006;44(5):538~544)

봉침요법 부위에 발생한 Mycobacterium chelonae 감염

심우행 ( Woo Haing Shim ),박현제 ( Hyun Je Park ),김훈수 ( Hoon Soo Kim ),진현우 ( Hyun Woo Chin ),김수한 ( Su Han Kim ),고현창 ( Hyun Chang Ko ),김병수 ( Byung Soo Kim ),김문범 ( Moon Bum Kim ),권경술 ( Kyung Sool Kwon ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.4

Mycobacterium chelonae is a rapidly growing atypical mycobacterium found in soil and water. In a healthy person, it causes cutaneous infection after an invasive procedure or surgery. Herein, we present a case of a 52-year-old man with multiple erythematous plaques and nodules on the back and left arm, and these occurred at the site of bee sting therapy by a herbal medical doctor. The histologic findings showed a granulomatous infiltration composed of numerous neutrophils, lymphocytes, eosinophils, histiocytes and multinucleated giant cells. Acid fast bacilli were detected by Ziehl-Neelsen stain and Mycobacterium chelonae infection was confirmed by an INNO-LiPA mycobacteria kit. The patient was treated with 1 g of clarithromycin and 200 mg of doxycycline for 6 months and his condition improved considerably. (Korean J Dermatol 2011;49(4):374∼378)