Cyclin A와 LATS 유전자들의 난소 내 mRNA 및 단백질 발현에 관한 연구

박창은 ( Chang Eun Park ),김대중 ( Dae Jung Kim ),홍성노 ( Sung No Hong ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.1

Despite of the importance of the primordial follicle (PMF) recruitment, factors and mechanisms for process are poorly understood. To evaluate expression and role of the follicular transition from PMF to PMF/primary follicles (PMIF) in the present study, we evaluated expression of lats1, lats2, cyclin A1, and cyclin A2 mRNA and protein, and elucidated and role of lats1-cyclin A in the follicular transition from PMF to PRIF. To analysis of differential expression in PMF and PMIF, each stage follicles were collected by day1 and day5 of immuno-compromised rats (ICR) and analyzed by real-time PCR for the genes. For localization of mRNAs and proteins of the genes, in situ hybridization and immunohistochemistry were performed. We confirmed that the lats1, lats2, cyclin A1, and cyclin A2 mRNA were more expressed in PMF than PMIF. Localization of the four genes expression were observed in nuclei of oocytes from the arrested primordial, and in the surrounding granulosa cells of the growing follicles. The mRNA expressions were gradually decreased with follicular development. From immunohistochemistry studies, Cyclin A1 protein expression were observed in oocyte cytoplasmas of early stage follicles, while observed in granulose cells and oocyte nucleoli during growing follicles. This study suggested that the presence of lats gene family might perform negatively regulation of cell proliferation by modulation of the CDC2/Cyclin A complex activity. lats-cyclin A genes in oocytes of the early stage follicles might play a role in the meiotic cell cycle arrest of the primary oocytes at the primordial follicle stage as well as the follicular growth.

Mobile transposon-like element, clone MTi7:RNA interference를 이용한 역할 규명

박창은,신미라,전은현,조성원,이숙환,김경진,김남형,이경아,Park, Chang-Eun,Shin, Mi-Ra,Jeon, Eun-Hyun,Cho, Sung-Won,Lee, Sook-Hwan,Kim, Kyung-Jin,Kim, Nam-Hyung,Lee, Kyung-Ah 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.4

Objectives: The present study was conducted to evaluate the mobile transposon-like element, clone MTi7 (MTi7) expression in the mouse ovary and to determine its role(s) in the mouse oocytes by RNA interference (RNAi). Methods: MTi7 mRNA expression was localized by in situ hybridization in day5 and adult ovaries. Double stranded RNA (dsRNA) was prepared for c-mos, a gene with known function as control, and the MTi7. Each dsRNA was microinjected into the germinal vesicle (GV) stage oocytes then oocyte maturation and intracellular changes were evaluated. Results: In situ hybridization analysis revealed that MTi7 mRNA localized to the oocyte cytoplasm from primordial to preovulatory follicles. After dsRNA injection, we found 43-54% GV arrest of microinjected GV oocytes with 68%-90% decrease in targeted c-mos or MTi7 mRNA. Conclusions: This is the first report of the oocyte-specific expression of the MTi7 mRNA. From results of RNAi for MTi7, we concluded that the MTi7 is involved in the germinal vesicle breakdown in GV oocytes, and MTi7 may be implicated with c-mos for its function. We report here that RNAi provides an outstanding approach to study the function of a gene with unknown functions.

Relationship of Stress Hormone and Neuroimmunological Response

Chang-Eun Park(박창은) 한국엔터테인먼트산업학회 2014 한국엔터테인먼트산업학회논문지 Vol.8 No.3

심리적 스트레스는 신경과 호르몬의 경로를 통해 면역 및 염증 세포의 기능을 조절할 수 있다. 스트레스는 또한 장관내 신경계의 내인계 인자에 의해 펩티드 호르몬 및 신경 전달 물질의 반응을 촉진시킨다. 세포 매개 면역 반응의 주된 근원은 백혈구이며 이로 인해 B세포, T 세포 그리고 이들로부터 방출되는 사이토카인이 가장 중요한 역할을 한다. 코르티솔과 연관된 사이토카인은 스트레스 매개성 면역 조절에 의해 방출된다. 스트레스에 의해 유도된 인자는 혈장에서 코르티솔을 증가시킨 반면에 인터루킨 (IL)-12/인터페론 (IFN)-γ, NK세포활성은 면역반응을 억제시킨다. 따라서 스트레스는 면역반응에 영향을 미치는 연관성이 있지만 향후 연구에서는 이들의 연관성을 위한 기전 연구가 요구된다. 향후, 스트레스와 관련된 면역반응의 질병관리 및 치료를 위한 바이오마커 적용의 구조체계에 대한 연구가 필요하다. Psychological stress can modulate immune and inflammatory cell function through neural and hormonal pathways. Stress also triggers the response of peptide hormones and neurotransmitters by intrinsic factors of the enteric nervous system. The major sources of cell-mediated immune response are leukocytes, whereby B and T cells and their release of cytokines play the most important role. A cortisol-related cytokine released by stress-mediated immune modulation. Stress-induced factors were increase in cortisol plasma levels, whereas, interleukin (IL)-12/Interferon (IFN)-γ and NK cell actiity were inhibits the immune system. Therefore, the job stresses are related to influenced immune responses but further research is needed to demonstrate for its mechanism relationships. Furthermore, the research needs framework the biomarker adoption for disease management, and treatment with stress related immune response.

프로칼시토닌의 수준에 따른 C-반응성단백과 혈구산정검사의 변화

김진산 ( Jin-san Kim ),박창은 ( Chang-eun Park ) 대한임상검사과학회 2022 대한임상검사과학회지(KJCLS) Vol.54 No.1

프로칼시토닌(procalcitonin, PCT)은 세균성과 바이러스성의 감염을 구별하기 위한 과학적 근거와 진단상의 중요한 단서를 제공한다. 프로칼시토닌이 높은 환자의 임상적 특성과 예후를 조사하고 임상적 진단을 향상시키고자 한다. 프로칼시토닌이 일반적인 집단에서 패혈증의 유병률과 연관성이 있는지를 결정한다. 이에 본 연구는 1년 동안 프로칼시토닌을 검사한 외래 환자(127명)의 C-반응성단백과 혈구산정검사의 결과를 기반으로 후향적 조사연구이다. 분석에 사용된 데이터는 프로칼시토닌, C-반응 단백 그리고 혈구산정검사이다. PCT의 결과가 높은 군의 CRP와 WBC의 양성율은 정상군보다 높게 나타났다(P<0.05). 프로칼시토닌 수준의 특이성과 민감도는 C-반응단백과 백혈구 수준보다 높게 나타났다(P<0.05). 또한. 프로칼시토닌의 결과값을 삼분위한 그룹의 분석에서는 낮은 군과 중간군의 값에 비해 높은 군의 값이 평균보다 높은 것으로 나타났다(P<.001). 상관분석에서는 프로칼시토닌은 C-반응단백, 백혈구(호중구, 림프구 포함)에서 양의 상관성을 보였다(P<.001). 이 연구의 주된 발견은 높은 프로칼시토닌은 C-반응성단백, 백혈구들의 수준에서 높은 연관성을 보이며 진단적 가치를 확인하였다. 따라서 이러한 관련 인자들은 환자의 감염진단과 항생제 치료에 중요한 진단적 및 치료적 영향을 미칠 것으로 사료된다. Procalcitonin (PCT) can provide an experimental rationale and a diagnostic lead to distinguish between bacterial and viral infections. This study sought to investigate the clinical characteristics and prognosis of patients with high PCT levels, to improve clinical diagnosis, and to determine whether PCT levels were associated with the subsequent development of sepsis in the general population. This was a retrospective observational study conducted on outpatients (N=127) over a year. The general data and laboratory parameters studied were PCT, C-reactive protein (CRP), and complete blood count (CBC). The positive rates of CRP and white blood cells (WBCs) in the elevated PCT group were higher than those of the normal group (P<0.05); the specificity and sensitivity of the PCT levels were obviously higher than those of the CRP and WBC levels at diagnosis (P<0.05). The mean PCT levels in the low group were significantly higher than those in the high or moderate group (P<0.001). There was a significant positive correlation with CRP, total WBCs, and neutrophils (P<.001). The main finding of this study was the significant association between an elevated PCT level and CRP and WBC levels, signifying a high diagnostic value. This has important implications for the diagnosis of bacterial infections and therapeutic implications for the use of antibiotic treatment in specific patients.

진공채혈관의 상태에 따른 평가

박창은 ( Chang Eun Park ) 대한임상검사과학회 2016 대한임상검사과학회지(KJCLS) Vol.48 No.2

분석전 변이로 인하여 검사실은 오류에 직면하게 되고 많은 요인들은 환자의 검사결과에 영향을 미치게 된다. 다양한 진공채혈관은 신속하게 분리하고 방치된 검체의 용혈을 방지한다. 그러나 진공채혈관의 상태에 따라 몇 가지 제한점들이 발생하는데 이러한 문제점을 알아보기 위해 다양한 진공채혈관을 이용해 혈구산정검사로 비교 평가하였다. 유통기한별 검사결과에서는 백혈구(WBC)와 혈소판(PLT)의 값에서 0.24, 0.21로 큰 변이계수를 나타냈고 상관성에서는 백혈구(WBC), 혈소판(PLT), 적혈구(RBC)의 값이 유의 성을 보였다( p<0.01). 한편 각 3사의 비교평가에서는 백혈구(WBC), 혈소판(PLT)의 값에서 0.27, 0.21로 가장 큰 변이계수를 나타냈고. 상관성에서는 각 3사가 모두 백혈구(WBC), 혈소판(PLT), 혈소판분포폭(PDW)의 값에서 높은 유의한 차이를 보였다( p<0.01) 그러나 G사와 B사는 혈소판(PLT), 혈소판분포폭(PDW)의 값에서는 낮은 유의한 차이를 보였다( p<0.05). 결론적으로 진공채혈관의 상태에 따른 분석의 안정성을 위해 적절한 진공채혈관의 분석평가가 이루어져야 한다. Pre-analytical variables account for most laboratory errors and many factors affect the results from a patient. Type of tubes facilitated rapid separation and prevented hemolysis upon prolonged storage. However, there were some limitations associated with vacutainer conditions. To circumvent the problems, the comparability of complete blood cell count values was examined using various vacutainers. The results of the analysis showed a large coefficient variation of 0.24, 0.21 in the value of white blood cells and platelets, and significant correlation was observed between white blood cells, platelets, and the value of red blood cells ( p<0.01). In each of the three tubes, compared to the value of platelets, white blood cells, the greatest coefficient variation was 0.27, 0.21. In correlation of the three companies, significant difference was observed in values of white blood cells, platelets, and platelet distribution width ( p<0.01), however G and B, the value of platelets, and platelet distribution width were significantly lower ( p<0.05). In conclusion, analysis of vacutainers showed that they were suitable for stability of these analytes under vacutainer conditions.

경주지방의 경주개 동경이(무미 또는 단미 형태의 개) 에서 혈액상 분석에 대한 실태조사

박창은 ( Chang Eun Park ),이은우 ( Eun Woo Lee ),성기창 ( Ki Chang Sung ),최석규 ( Seog Gyu Choi ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.4

Investigations for hematologic and biochemical values for Gyeongju DongGyeong dogs were performed. This study were conducted to determine normal blood and serum chemical values on DongGyeong dogs. Normal blood and serum chemical values were studied in 47 healthy dogs (male 28, female 19). The hematologic and serum chemistry results were confirmed by age, sex, shape of tail. The hematological values showed no difference from Korea native dogs. The hematocrit values (49.0±8.1(%)) of DongGyeong dogs (2-3 years group) were significantly higher than other dogs. The mean levels of MCV (61.6±3.8(?m3)) in DongGyeong dogs were lower than general dogs. The levels of cholesterol(220±80.5(mg/dl)) and inorganic phosphorus (3.6±1.0 (mg/dl)) of DongGyeong dogs (2~3 years group) were significantly higher than Korea native dogs. Totally concentration of triglyceride (66.2±17.0 (mg/dl)) of DongGyeong dogs were significantly lower than Korea native dogs. The mean values of BUN were increased with age but no significantly. In conclusion, data obtained from this study may be valuable as a standard for interpretation of the results in hematologic and biochemical analysis of Gyeongju DongGyeong dog populations.

경주지방의 무미 또는 단미 형태의 개(경주개 동경이)의 혈청 단백질의 분획에 대한 실태조사

박창은 ( Chang Eun Park ),이은우 ( Eun Woo Lee ),성기창 ( Ki Chang Sung ),최석규 ( Seog Gyu Choi ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.3

Serum proteins of Korean native dogs (Gyeongju DongGyeong dogs) was analyzed by capillary electrophoresis system. Electrophoretic patterns showed six fractions. Total serum protein and ratio of albumin to globulin were 5.99±0.83 (g/dl) and 1.41±0.29 (g/dl) in DongGyeong dogs. Relative percentages of total serum albumin and α-1, α-2, β-1, β-2, γ-globulin fraction were 57.94±5.43, 3.15±2.30, 7.49±4.09, 9.43 ±3.50, 7.63±5.70, and 14.36±7.63, respectively. It was observed that β-globulin was higher than other fractions. The most striking alternation with age was founded in the γ-fractions. Also, it was observed that ratios of albumin to globulin in DongGyeong dogs were higher than on other dogs.

울산지역 개의 심장사상충 감염에 관한 조사

박창은 ( Chang Eun Park ) 한국가축위생학회 2010 韓國家畜衛生學會誌 Vol.33 No.2

A survey of canine heartworm infections among 83 dogs (Male 42, Female 41) of pet dogs in Ulsan area was performed from March to July 2009 using antigen test. Of 83 dogs, 6 dogs (7.2%) were infected with the Dirofilaria immitis, and 5 dogs (6%) were detected to have microfilaria in the peripheral blood. The infection according to the age of 4-5, 6-7, 7 over years were 7.4%, 12.5%, and 10.5%, Also, A survey of canine heartworm infections among 23 dogs (Male 15, Female 8) of hunting dogs in Ulsan area was performed from July 2008 using antigen test. Of 23 dogs, 11 dogs (47.8%) were infected with the D. immitis, and 6 dogs (26.1%) were detected to have microfilaria in the peripheral blood. The infection according to the age of under 1, 2-3, 4-5, 6-7 over years were 12.5%, 40%, 85.7% and 66.7%, respectively.

알레르기 환자에서 한국의 대표적 흡입성 항원 16종의 특이 IgE 측정: GENEDIA-ELISA (Genedia Inhalant Allergen ELISA)법과 MAST-Immunoblot (RIDASCREEN)법 결과 비교

박창한 ( Chang Han Park ),권용은 ( Yong Eun Kwon ),김덕인 ( Deok In Kim ),권혁수 ( Hyouk Soo Kwon ),손성욱 ( Seong Wook Sohn ),박흥우 ( Heung Woo Park ),장윤석 ( Yoon Seok Chang ),김선신 ( Sun Sin Kim ),성기민 ( Ki Min Seong ) 대한천식알레르기학회 2007 천식 및 알레르기 Vol.27 No.1

Background and Objective: A novel multiple antigen simultaneous test using ELISA assay (GENEDIA-ELISA) has been developed. It includes two spotted spider mite (TSM) and Japanese Hop pollen (Hop J), which are currently common allergens in Korea but were not readily available for test in the past. The aim of this study was to validate GENEDIA-ELISA assay and to compare it with MAST-Immunoblot assay which was one of the commonly used in vitro tests in Korea. Method: One hundred eight patients were evaluated with skin prick tests, MAST-Immunoblot and GENEDIA-ELISA assay. Using skin prick test results (A/H ratio>1) as the gold standard, the sensitivity, specificity, positive predictive value, and negative predictive value of GENEDIA-ELISA (cut-off=class 2) and MAST-Immunoblot (cut-off=class 2) were examined. The results were analyzed using receiver operating characteristic (ROC) analysis. Result: The overall sensitivity of GENEDIA-ELISA was higher than that of MAST-Immunoblot (61.4% vs. 38.4%; P<0.05) while the overall specificity of each test was similar (88.5% vs. 93.9%). There was a higher correlation between GENEDIA-ELISA and skin prick tests than that obtained between MAST-Immunoblot and skin prick tests (0.47 vs. 0.39). In ROC analysis, the overall result of GENEDIA-ELISA yielded an area under the ROC curve (AUC) of 0.75, which indicated that this assay is more accurate test than MAST-Immunoblot (AUC=0.66). The sensitivities for TSM and Hop J on GENEDIA-ELISA were 83.3% and 83.3%, respectively, and AUC were 0.73 and 0.89, respectively. Conclusion: GENEDIA-ELISA assay may be a sensitive and acceptable tool of specific IgE against common inhalant allergens in Korea. (Korean J Asthma Allergy Clin Immunol 2007;27:26-32)

류마티스 관절염 진단을 위한 검사의 유용성 비교

박창은 ( Chang Eun Park ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

류마티스 관절염은 주로 관절의 활막 세포의 증식, 병리학적 면역 반응과 관절의 진행성 파괴를 특징으로 하는 만성 염증성 질환이다. 이 질환은 중요한 사회적 건강 문제로 대두된다. 이 논문을 통해 분자수준에서 변화와 류마티스 관절염의 진단 또는 질병의 진행에 대한 발병 기전을 새로운 관점에서 발병기전을 제공한다. 또한 조기 진단과 류마티스 관절염의 예후에 도움이 될 것으로 보인다. 새로운 혈청학적 및 면역학적 바이오마커에 초점을 맞추고 있다. 따라서 넓게 질병 진행의 위험 환자를 식별하는 혈청학적, 생체 면역학적 요인 등을 규명하고 적용시키는 것을 도출할 수 있다. 진단검사 의학을 기반으로 하는 증거는 환자에게 최선의 결과를 제공할 수 있다. 마지막으로. 최근의 연구 데이터는 이 접근을 통해서 치료를 최적화하기 위해 조기 진단 및 치료에 도움이 되는 새로운 접근 방식으로 궁극적인 유용성을 정립하는데 도움이 될 것으로 사료된다. Rheumatoid arthritis (RA) is a chronic inflammatory disease, which is mainly characterized by disease of joints affected with synovial hyperplasia, pathological immune response, and progressive destruction; all of which represent an important social health problem. These provide new insights in its pathogenesis of rheumatoid arthritis diagnosis and disease progression in molecular changes. This review focuses on new serological and immunological markers which seem to be useful in early diagnosis and prognosis of rheumatoid arthritis. Therefore, such tests are widely conducted for serological biomarkers and the developments with such immunological factors to identify patients who are at risk for disease progression. This evidence of the disease based on laboratory medicine could provide the best outcome for patients. Finally, data from recent studies will help to refine the ultimate usefulness of this novel approach for early diagnosis, treatment, and helping clinicians to optimize therapy by using this approach.