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Dry-Gel Hybridization with Synthetic Oligonucleotides Complementary to Human Insuline Receptor Gene
박종상,Park, Jong-Sang 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.2
사람 인슐린 수용체 유전자에 상보적인 짧은 합성 올리고뉴크레오타이드를 사용하여 건조-젤 막에 직접 hybridization시켰다. 씻는 온도를 $60^{\circ}C$ 근처로 맞추면 사람 인슐린 수용체 유전자에 해당하는 DNA의 한 밴드를 검출할 수 있었다. 건조-젤 법은 간단하고 시간이 절약되며 southern blotting 만큼 감도가 좋다. Synthetic oligonucleotides complementary to the human Insulin Receptor gene was hybridized with dry-agarose gel membrane directly without DNA blotting. A single band of human insulin receptor gene can be detected with the proper washing temperature. This technique is simple, time-saving and as sensitive as southern blotting to detect a single copy gene.
박종상,이정학 ( Jong Sang Park,Chung Hak Lee ) 한국공업화학회 1991 공업화학 Vol.2 No.4
저장 안정한 액체염료의 제조를 위하여 새로운 분리 기술을 도입하였다. 액체염료에 함유된 무기염의 종류, 농도 및 액체염료의 저장기간이 염료분자의 집합(aggregation)과 안정성에 미치는 영향을 실험측정하였다. 높은 염농도에 의한 염료분자의 집합의 형성을 방지하기 위해 표면처리한 중공사막을 이용한 역확산(counter diffusion) 법을 도입하여 액체염료에 함유된 염을 선택적으로 제거하였다. 역확산에 의한 염의 제거 효율은 1회 통과에 5-20%였고 염과 염료의 분리비는 10-700으로 매우 큰 값을 갖는 반면 염료의 손실율은 0.4%이하였다. 모델식을 이용하여 구한 염과 염료의 막에 대한 물질전달 계수 (U_(M, Na,) U_(M, Dye))는 표면처리한 경우에 각각 2.75와 0.72 l/㎡/hr의 값을 나타내어 염과 염료의 혼합액에서 염의 선택적 분리가 가능함을 보여주었다. 또한 탈거수 및 공급액의 유속, 막의 막힘현상이 염의 제거 호율에 미치는 영향을 연구하였다. New separation process was developed for the preparation of storage-stable liquid dyes. The extent of aggregation of dye molecules was measured with respect to storage time of liquid dyes under different salt environments. Hollow-fiber membranes were modified by immobilization of inorganic crystals onto the surface of membrane. Using surface-treated membranes, counter diffusion technology was introduced to selectively remove salts from dye solution. The separation factors were 10-770, and the less of dye molecules was less than 0.4 %. Membrane permeabilities for sodium ions(U_(M, Na)) and dye molecules(U_(M, Dye)) were found to be 2.75 and 0.72 l/㎡/hr, respectively, in the case of surface-treated membranes. The effects of various operating parameters on desalting efficiency were also investigated.
박종상,이태원,하종률,정성식,Park, Chong-Sang,Lee, Tae-Won,HA, Jong-Yul,Chung, Sung-Sik 대한기계학회 2000 大韓機械學會論文集B Vol.24 No.3
As the combustion process of CWM consists of the water evaporation, the release and combustion of volatile matter, and the combustion of char for every particle, it is more complex than that of existent liquid fuel. Though the many studies on CWM combustion have been carried out by the single droplet using hanging methods or the multiple droplet using atomization methods, any report don't presents definite solution about the effects by the initial water evaporation and combustion of volatile. When CWM is suddenly exposed in the high temperature surroundings, the internal water evaporates and then each droplet builds up pores. Besides, porosity rate changes along the temperature of surroundings, the composition ratio of CWM, and the initial diameter of droplet. In result, because it affects the whole combustion rate, the combustion of CWM has complex mechanism as compared with the combustion of liquid or gas fuel. Therefore, concentrating on porous structure of CWM, this study has proceeded to acquire the basic data on the CWM injection combustion and closely examines the effects of the first stage combustion on the whole combustion by measuring the diameter variations, pore rate, mass fraction burned, and the internal temperature changes of CWM droplet. The results demonstrate that $60{\sim}70%$ of initial mass is reduced during water evaporation and volatile combustion period, and swelling rate, mass faction burned, and density variation are greatly concerned with atomization of CWM etc.
박종상,성창근,장기운,Park, Jong-Sang,Sung, Chang-Geun,Chang, Ki-Woon 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1
알로에 술의 품질향상 및 산업화를 위한 연구자료로서 알로에 술의 제조법을 과학화하기 위하여, 알로에 첨가량에 따른 알로에 술의 특성과 지표성분 함량변화를 살펴본 결과는 다음과 같다. 발효과정 중의 알콜 생산은 0.5%의 알로에 분말 첨가구에서 당을 15%, 20%, 25%로 증량시 약 6.3%, 9.6%, 그리고 11.3%의 알콜이 각각 생성되었다. 술덧중에 알로에의 첨가량이 늘어나면서 알콜의 생산이 완만하게 저하되었다. 알로에의 지표성분으로서 알려져 있는 barbaloin은 0.5% 알로에 첨가구에서 barbaloin의 양은 발효 7일 후 4.2 mg/ml, 10일후에는 4.6 mg/ml이었으나, 30일 후에는 다시 감소되었다. 알로에의 첨가량에 따른 기호도로서 알로에의 쓴맛에도 불구하고 1.5%와 2.0%의 알로에 첨가가 높은 선호도를 보였다. For the scientific approaches and quality control of aloe wine as fundamental studies of industrialization and quality improvement, change of barbaloin content during the fermentation period and various characteristics of aloe wine were investigated. Alcohol of 6.35, 9.6%, and 11.3% in the 0.5% concentration of aloe powder was produced from 15%, 20%, and 25% addition of sugar in the wine mash, respectively, As the content of aloe powder is increased, production of alcohol was slightly decreased, indicating aloe powder might contain antimicrobial activity. The content of barbaloin in the 0.5% concentration of aloe powder was 4.2 mg/ml, 4.6 mg/ml and 2.21 mg/ml after 7, 10 and 30 day, respectively. The tasty characteristics of aloe wine brewed with aloe powder of 1.5% and 2.0% were most acceptable to the sensory panels.
사람 인슐린 수용체 유전자에 상보적인 합성 DNA 를 사용한 건조 젤 hybridization
박종상 ( Jong Sang Park ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.2
Synthetic oligonucleotides complementary to the human Insulin Receptor gene was hybridized with dry-agarose gel membrane directly without DNA blotting. A single band of human insulin receptor gene can be detected with the proper washing temperature. This technique is simple, time-saving and as sensitive as southern blotting to detect a single copy gene.
박종상(Park, Jong-sang) 국방부 군사편찬연구소 2018 군사 Vol.- No.107
Since the establishment of the Marine Corps of Republic of Korea, the locations of the HQ has been changed a lot and many units have created and expanded over the time, and they have been lost due to the adjustment of the organization. And new units were created, and existing units moved to new places. It is not easy to find the positions of the troops that were formed in the early days of the army due to such a large number of changes. It is because many places do not appear at the time of the formation of the unit. It is located on Jeju Island, which is the second station after the foundation, in front of Joil Pharmacy at Gwangdeok-ro. It is equipped with a marker that the Marine Corps HQ. is stationed on. However, the position and the position of the marker should be more thoroughly researched historically. Former Defense Minister Kim Seong-eun, who was Marine Corps Commander’s Chief of Staff at the time, arrived at Jeju Island when the Marine Corps arrived at Jeju Island, where he visited the Battalion headquarters of the Army’s 1st Independence Battalion, and received orders from Colonel Kim Yong-Ju And that he used the two-story wooden building used by the army. Because Jeju Island was the 1st Army Independent Battalion that was assigned to the 2nd Army Army. Thus, the Marines were taken over from the 1st Army Independent Battalion on December 28, 1949. At that time, the 1st Army Independent Battalion had a commanding post in the Jeju agricultural high school, where the 2nd Army Rgt. hq. was located, and the Marine Corps was stationed after the takeover of this area. There was a Marine Corps HQ. at 283 Samdo-ri (currently located at Jeju International Education Information Center), which was located in the Jeju agricultural High School at that time. Therefore, it should be installed at Jeju International Education Information Center, which is the location of the old Jeju agricultural high school where the Marine Corps HQ. was located since December 28, 1949. After the emergency martial law was declared on July 8, 1950, the Marine Corps HQ. was located in Gwandeokjeong. Also, the contents of marker should be corrected.