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배양한 사람 사구체 내피세포의 Cytokine자극에 따른 접착 분자 및 HLA-DR 발현의 연구
박수길(Su Kil Park),양원석(Won Seok Yang),안한종(Han Jong Ahn),김청수(Choung Soo Kim),이종수(Jong Soo Lee),이재담(Jae Dam Lee) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.2
N/A Background: Glomerular endothelial cells should participate in the process of glomerular disease by expressions of HLA antigens and adhesion molecules. However, few have been known about the regulation of the expression of these molecules in human glomerular endothelial cells(HGEC). The aim of this study was to evaluate the expressions of VCAM-1, ICAM-1 and HLA-DR to see if there are any cytokine-dependent or time-dependent differences. Methods: HGEC were isolated and cultured from the normal portion of the kidneys removed due to renal cell carcinoma, which was confirmed by factor VIII and fluorescent-labeled acetylated LDL. The effects of cytokine on the cell surface expression of VCAM-1, ICAM-1 and HLA-DR were measured by ELISA. Results : ICAM-1 was increased by IL-1 α, TNF- β and IFN- γ. VCAM-1 was increased by IL-1 α and TNF- β, not by IFN- γ. IFN- γ only increased expression of HLA-DR. Basal expression of ICAM-1 was higher than VCAM-1 and HLA-DR. The time course of expression was different according to adhesion molecule. Conclusions : These data showed that the expression of adhesion molecules and HLA-DR in HGEC were regulated differentially by inflammatory and immune-regulatory cytokines.
조성우,박수길,김금이,이희봉,이재담,Cho, Sung-Woo,Park, Su-Kil,Kim, Geum-Yi,Lee, Hee-Bong,Lee, Jae-Dam 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3
쥐의 mesangial 세포를 초회 배양하여 Transforming Growth Factor-$\beta$(TGF-$\beta$)가 세포의 복제 및 collagen 합성에 미치는 영향을 조사하였다. TGF-$\beta$는 사람 혈소판에서 산성 에탄올 침전법 및 FPLC를 이용한 젤 크로마토그래피의 방법으로 정제되었다. TGF-$\beta$가 mesangial 세포를 복제 및 collagen 합성에 미치는 영향은 TGF-$\beta$ 농도와 세포의 밀도에 따라 달라졌다. Confluent 세포의 경우 0.1~15 ng/ml의 농도에서 TGF-$\beta$는 mesangial 세포의 복제를 35%까지 감소시켰다. Collagen 합성은 0.5 ng/ml의 TGF-$\beta$ 농도에서는 2배로 증가되었으나 그 이상의 농도에서는 감소하기 시작하는 두 가지의 조절 양상을 보였다. 그러나 subconfluent 세포의 경우에는 세포의 복제 빛 collagen 합성 모두에 TGF-$\beta$는 아무런 효과를 나타내지 못하였다. 본 연구결과는 TGF-$\beta$가 glomerulosclerosis에 있어서 중요한 역할을 하리라는 가능성을 제시해주고 있다. The effect of TGF-$\beta$ on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-$\beta$ was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-$\beta$ on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-$\beta$ concentration and cell density in monolayer culture. Concentrations of TGF-$\beta$ ranging from 0.1~15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-$\beta$ concentrations in the range 0.1~15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-$\beta$ on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-$\beta$ induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-$\beta$ occurred at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-$\beta$ with the effects on collagen production. In subconfluent cells, however, TGF-$\beta$ showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-$\beta$ may play an important role as a multifunctional regulator in the pathogenesis of glomerulosclerosis.
사람 혈소판에서 분리한 Transforming Growth Factor - β가 초회 배양된 Mesangial 세포의 복제 및 Collagen 합성에 미치는 영향
조성우,박수길,김금이,이희봉,이재담 ( Sung Woo Cho,Su Kil Park,Geum Yi Kim,Hee Bong Lee,Jae Dam Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.3
The effect of TGF-β on cell replication and collagen synthesis in primarily cultured mesangial cells from rats was studied in vitro. The TGF-β was purified from human platelets by acidic ethanol extraction and gel filtration with FPLC. The effect of TGF-β on the DNA synthesis and the collagen synthesis in mesangial cells depended on both TGF-β concentration and cell density in monolayer culture. Concentrations of TGF-β ranging from 0.1∼15 ng/ml were evaluated for their effects upon DNA synthesis. In confluent cells, when compared to the growth rate in standard medium, TGF-β concentrations in the range 0.1∼15 ng/ml reduced a cell proliferation up to 35%, suggesting inhibition of cell division. The effect of TGF-β on collagen synthesis in mesangial cells was biphasic. In confluent cells, the addition of TGF-β induced a 2-fold increase in the production of collagen when compared with cells grown in standard medium. The maximal effect of TGF-β occurred at 0.5 ng/ml. At higher concentrations there was a decrease in the action of TGF-β with the effects on collagen production. In subconfluent cells, however, TGF-β showed no significant effects on the DNA synthesis and the collagen synthesis at all concentrations. These results support that TGF-β may play an important role as a multifunctional regulator in the pathogenesis of glomerulosclerosis. v
부갑상선호르몬 분비가 저하된 혈액투석 환자에서 저칼슘투석액 사용시 칼슘감지수용체의 유전자 다형성에 따른 호르몬 분비의 변화
박태진 ( Tae Jin Park ),서장원 ( Jang Won Seo ),백관미 ( Kwan Mi Pack ),장재원 ( Jai Won Chang ),양원석 ( Won Seok Yang ),김순배 ( Soon Bae Kim ),이상구 ( Sang Koo Lee ),박수길 ( Su Kil Park ),박정식 ( Jung Sik Park ) 대한신장학회 2007 Kidney Research and Clinical Practice Vol.26 No.4
Purpose : Low level of parathyroid hormone (PTH) is a risk factor that might cause hip fracture in dialysis patients. Low calcium dialysate (LCD) has been suggested as an approach to increase PTH level. The calcium- sensing receptor (CaSR) polymorphism is known to be associated with the sensitivity to extracellular calcium. Methods : We prospectively investigated the role of genetic polymorphism of CaSR codon 990 as one cause of the different parathyroid responses to LCD in maintenance hemodialysis (HD) patients. 48 patients, using 3.5 mEq/L calcium dialysate, with intact PTH below 100 pg/ml for the last one year underwent HD sessions on 2.5 mEq/L calcium dialysate for 12 weeks. Serum intact PTH, total calcium, phosphorus, alkaline phosphatase (ALP) and bone-specific ALP (BAP) were measured monthly. The CaSR gene from peripheral lymphocytes was amplified to confirm the genotype by polymerase chain reaction. Results : According to the CaSR genetic polymorphism, subjects were divided into 3 groups, A/A (14.6%), A/G (45.8%) and G/G (39.6%). Twelve weeks later, intact PTH (48.5±25.4 to 89.0±49.4 pg/mL, p<0.01), and ALP (78.7±25.7 to 87.4±27.2 IU/L, p<0.01) increased significantly in G/G group, but not in non-G/G groups. However, BAP significantly increased in both G/G group (24.3±11.9 to 29.5±10.6 U/L, p<0.01) and in non-G/G groups (21.4±4.5 to 26.1±9.1 U/L, p<0.01). During the study period, levels of corrected total calcium and phosphorus were not significantly changed. Conclusion : The CaSR polymorphism, G/G genotype, strongly influenced the responsiveness of parathyroid gland to LCD, compared with non-G/G genotypes. However, bone formation may occur actively on LCD, irrespective of CaSR genetic polymorphism.