배양한 사람 사구체 내피세포의 Cytokine자극에 따른 접착 분자 및 HLA-DR 발현의 연구

박수길(Su Kil Park),양원석(Won Seok Yang),안한종(Han Jong Ahn),김청수(Choung Soo Kim),이종수(Jong Soo Lee),이재담(Jae Dam Lee) 대한신장학회 2001 Kidney Research and Clinical Practice Vol.20 No.2

N/A Background: Glomerular endothelial cells should participate in the process of glomerular disease by expressions of HLA antigens and adhesion molecules. However, few have been known about the regulation of the expression of these molecules in human glomerular endothelial cells(HGEC). The aim of this study was to evaluate the expressions of VCAM-1, ICAM-1 and HLA-DR to see if there are any cytokine-dependent or time-dependent differences. Methods: HGEC were isolated and cultured from the normal portion of the kidneys removed due to renal cell carcinoma, which was confirmed by factor VIII and fluorescent-labeled acetylated LDL. The effects of cytokine on the cell surface expression of VCAM-1, ICAM-1 and HLA-DR were measured by ELISA. Results : ICAM-1 was increased by IL-1 α, TNF- β and IFN- γ. VCAM-1 was increased by IL-1 α and TNF- β, not by IFN- γ. IFN- γ only increased expression of HLA-DR. Basal expression of ICAM-1 was higher than VCAM-1 and HLA-DR. The time course of expression was different according to adhesion molecule. Conclusions : These data showed that the expression of adhesion molecules and HLA-DR in HGEC were regulated differentially by inflammatory and immune-regulatory cytokines.

日本의 敎育実態와 基督敎敎育의 課題

朴壽吉(Soo. Kil Park) 연세대학교 신과대학(연합신학대학원) 1993 신학논단 Vol.21 No.-

신침범의 임상증상이 없는 낭창성 신염의 임상 및 병리소견 분석

박수길(Soo Kil Park),이종호(Jong Ho Lee),한진석(Jin Suk Han),김성권(Suhng Gwon Kim),최성재(Sung Jae Choi),이정상(Jung Sang Lee),이현순(Hyun Soon Lee),김용일(Yong Il Kim) 대한내과학회 1988 대한내과학회지 Vol.34 No.5

N/A Because of the limited validity of serologic tests, there has been interest in alternative ways of assessing prognosis and disease severity in systemic lupus erythematosus(SLE), espec ally in direct histopathologic evaluation of affected tissue. Since the renal disease is still a major cause of morbidity and mortality in SLE and the kidney is the most accessible organ by biopsy, the role of renal biopsy in the management of SLE has been discussed frequently. It is known that despite of the absence of clinical abnormalities of urine, histological examination of renal tissue has been shown variable morphological changes including diffuse proliferative type that has the worst prognosis in lupus nephritis. To investigate the usefullness of renal biopsy and the treatment modalities we performed the analysis of clinical and pathological findings in lupus nephritis without clinical renal involvement. Among the 95 patients of SLE, the number of silent lupus nephritis was 23, who were less hypertensive and clinically more favorable. The decrease of C3 and C4 was more marked in the patients who had the renal symptoms clinically. The severity of extrarenal symptoms was not correlated with whether the patient had the renal symptoms or not. The pathologic classification in silent lupus nephritis was as followings; WHO class IIa;8, IIb;7, class IIl;4, class IV;3, class V;1 respectively. Thus we concluded that the diffuse lupus glomer-ulonephritis could be clinically silent and the type of morphologic lesion in lupus nephritis cannot be deduced from the clinical criteria alone. It may be necessary to perform the biopsy in any SLE patient, regardless of the clinical renal involvement.

유한요소법에 의한 평면 TV 새도우마스크의 열변형해석 및 전자빔 오착 예측

김정,박수길,강범수,Kim, Jeong,Park, Soo-Kil,Kang, Beom-Soo 대한기계학회 2002 大韓機械學會論文集A Vol.26 No.11

Two-dimensional and three-dimensional finite element methods have been used to analyze the deformation behavior of a shadow mask due to thermal and tension load. The shadow mask inside the Braun tube of a TV set has numerous slits through which the electron beams are guided to land on the designed phosphor of red, green or blue. Its thermal deformation therefore causes landing shift of the electron beam and results in decolorization of a screen. For the realistic finite element analysis, the effective thermal conductivity and the effective elastic modulus arc calculated, and then the shadow mask is modeled as shell without slits. Next a transient thermal analysis of the shadow mask is performed, wherein thermal radiation is a major heat transfer mechanism. Analysis of the resulting thermal deformation is followed, from which the landing shift of the electron beam is obtained. The present finite element scheme may be efficiently used to reduce thermal deformation of a shadow mask and in developing prototypes of a large screen flat TV.

속발성 부갑상선 기능항진증의 수술적 치료경험 2예

홍석준,박수길<SUP>1<.SUP>,공경엽<SUP>2<.SUP>,Suck Joon Hong,Soo Kil Park,<SUP>1 <.SUP>and Gyungyub Gong,<SUP>2<.SUP> 대한갑상선-내분비외과학회 2003 The Koreran journal of Endocrine Surgery Vol.3 No.1

혈액투석 중 채취한 검체를 이용한 Equilibrated Kt/V 를 예측하는 방법

이종수(Jong Soo Lee),김도하(Do Ha Kim),최승원(Seung Won Choi),김영일(Young Il Kim),박재후(Jae Hoo Park),박수길(Su Kil Park),이상구(Sang Koo Lee),이선호(Seon Ho Lee),김성률(Sung Ryul Kim),박정식(Jung Sik Park) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.1

목 적 : 단일 구획 요소 역동학 모형에서 투석 직후 혈중 요소질소 농도의 반등(postdialysis urea ebound, PDUR)은 실제 Kt/ V를 과대 평가하게 된다. 본 연구는 투석 후 평형상태의 혈중 요소질소 농도를 가장 잘 반영하는 투석 중의 혈액 검체 채취 시점을 확인하고, 이 검체를 이용하여 계산된 Kt/ V가 요소질소 농도 반등 완료 후의 Kt/V와 일치하는 정도를 알아보고자 하였다. 방 법 : 유지혈액 투석을 받고 있는 21명의 말기 신부전증 환자에서 투석 전, 투석시작 후 80분, 120분, 190분, 200분, 210분, 220분, 투석종료 시점, 투석종료 45분 후 혈액을 채취하여 각각 BUN을 측정하였다. 저자들은 투석종료 45분 후 요소질소 농도 반등이 완료된 시점의 검체를 이용한 Kt/V(eKt/V PDUR)를 참고치로 하여 Smye method, double- pool Daugirdas 공식, Tattersall 공식, 투석 중에 채취한 검체 중 하나를 이용하여 계산한 Kt/V를 각각 비교하였다. 결 과 : 투석 후 평균 요소질소 농도의 반등은 17.2±6%였고 single poool (sp)Kt/ V와 eKt/V는 각각 1.300±0.24, 1.120±0.20으로 spKt/ V는 eKt/ V PDUR를 13.5±4% 과대평가 하였다. 투석 중에 채취한 검체 중 투석종료 40분 전에 채취한 검체가(27.9±9 ㎎/dL) 요소농도 반등후의 평형상태의 혈중요소 질소 농도(28.0±9 ㎎/dL)를 가장 잘 반영하였다. Double- pool Daugirdas 공식의 Kt/V(eKt/V Dau =1.135±0.21, r =0.972, p<0.001), Tattersall 공식의 Kt/V(eKt/ V T at =1.134±0.22, r =0.972, p<0.001), Smye공식의 Kt/ V(eKt/ V Smye=1.156±0.24, r =0.900, p<0.001)는 PDUR eKt/V과 유의한 상관관계를 보였으며, 투석종료 40분 전에 채취한 검체를 이용하여 계산한 Kt/ V(eKt/V T-40 min =1.120±0.20, r =0.984, p<0.001)가 가장 유의한 상관관계를 나타내는 경향을 보였다. Bland- Altman분석에서 Kt/V T - 40 min는 eKt/ V PDUR과 가장 좋은 일치 정도를 보였다. 결 론 : 일반 투석기를 이용한 4시간 동안의 혈액투석에 있어서 spKt/V는 실제 전달된 Kt/V를 평가하는데 부적절하며, 투석종료 40분 전에 채취한 검체를 이용하여 계산한 Kt/V는 요소질소 농도 반등 후의 평형상태의 Kt/ V를 예측하는데 있어서 임상적으로 간단하게 이용할 수 있는 좋은 방법으로 생각된다. Purpose : Postdialysis urea rebound(PDUR) causes the overestimation of actual amount of delivered dialysis in Kt/V from single pool urea kinetic. To correct PDUR and predict equilibrated Kt/V (eKt/V) some methods have suggested by Daugirdas, et al. Smye, et al. and Tattersall, et al. The purposes of this study were to determine the optimum intradialytic urea sample time that fits best with PDUR and to compare calculated Kt/V by this sample with the different other methods. Methods : The subjects were 21 patients who were dialyzed at three times 4 hours weekly, using bicarbonate and cellulosynthetic membranes. Blood samples to measure BUN were obtained at initiation of dialysis session and then at 80, 120, 180, 200, 210, 220 minutes, and the end of dialysis session times and then at 45 minutes of postdialysis . We compared four different methods of eKt/V with the equilibrated 45-minute PDUR Kt/V (eKt/V PDUR) as the reference method. Results : The mean PDUR was 17.2± 6%. spKt/V and eKt/V PDUR was 1.300±0.24 and 1.120±0.20 respectively, leading to overestimation of actual delivered Kt/V by 13.5±5%. The best time for intradialytic sampling to fit with PDUR BUN w as 40 minute before the end of session. eKt/V by Daugirdas formulae(eKt/V Dau=1.315± 0.21, r=0.972, p<0.001), Tattersal formulae(eKt/V T at=1.134± 0.22, r =0.972, p<0.972, p<0.001) and Smye formulae(eKt/V Smye=1.156± 0.24, r=0.900, p<0.001) showed good correlation with eKt/V PDUR. eKt/V calculated by the sample of 40 minute before end of session (eKt/V T-40=1.120±0.20, r=0.984) had tendency of the best correlation with eKt/V PDUR. Among these different methods, eKt/V T -40 had the best degree of agreement with eKt/V PDUR by Bland-Altman analysis. Conclusion : Our results suggest that the use of spKt/V is not adequate to estimate the amount of delivered hemodialysis dose because of the existence of significant postdialysis urea rebound even conventional 4 hour dialysis. Intradialytic sampling method is a simple and accurate method to predict eKt/V for use in clinical practice.

유한요소법에 의한 평면 TV 새도우마스크의 마이크로포닉 현상 해석

김정(Jeong Kim),박수길(Soo-Kil Park),강범수(Beom-Soo Kang) Korean Society for Precision Engineering 2002 한국정밀공학회지 Vol.19 No.11

사체 신이식에서 공여자의 상태가 이식신에 미치는 영향

김삼정(Sam Jeoung Kim),박광재(Kwang Jae Park),이종수(Jong Soo Lee),양원석(Won Seok Yang),이상구(Sang Koo Lee),한덕종(Duck Jong Han),박정식(Jeong Sik Park),박수길(Su Kil Park) 대한내과학회 1997 대한내과학회지 Vol.53 No.4

N/A Background: Kidney transplantation from cadaveric donor is increasing in KOREA. Knowledge of donor factors that can influence early graft function should be an important guideline in determining the suitability of a donor kidney for transplantation. Method: From June 1989 to August 1995, sixty patients with end-stage renal disease underwent kidney transplantation from thirty-two cadaver donors in Asan Medical Center. In this study, we reviewed the clinical characteristics of the cadaver donors including vital sign, urine output, serum creatinine, amounts of vasopressor needed, as well as the cause of brain death and evaluated the effect of these parameters on the early post-transplantation graft function (up to 6 months). Results: The data are given as median (range). The donors had been admitted for 40 hours (12-177) before nephrectomy. During admission, the lowest systolic blood pressure was 85mmHg (60-130). Platelet count was 112×10³/L (11-270×10³). Hour urine output was 447ml/h (212-937). These parameters did not have significant effects on the early post-transplantation graft function of our patients. Fifteen of thirty two donors had peak serum creatinine higher than 1.4mg/dl (mean 1.9㎎/dl, range 1.5-3.6㎎/dl) with serum creatinine 1.5㎎/dl (1.5-2.0) at the time of nephrectomy. The declining tendency and lowest level of serum creatinine after renal transplantation in patients who received kidneys from donor with peak serum creatinine higher than 1.4㎎/dl was not different from those in patients who received kidney with normal function. Conclusion: The transient hypotension or acute reversible mild to moderate deterioration of renal function in cadaver donor does not seem to have significant effect on the early post-transplantation graft function if underlying organic renal disease could be ruled out by careful history taking and clinical evaluation.

연속계 해석에 의한 보오링 바의 동적 거동에 관한 연구

김정석(Jeong-Suk Kim),강명창(Myeong-Chang Kang),박수길(Soo-Kil Park) Korean Society for Precision Engineering 1994 한국정밀공학회지 Vol.11 No.4

혈액투석중인 만성 신부전증 환자의 피부증상 및 이에 대한 혈액투석의 영향

성경제(Kyung Jeh Sung),이미우(Mi Woo Lee),최지호(Jee Ho Choi),고재경(Jai Kyoung Koh),박수길(Soo Kil Park) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.3

The purpose of this study was to examine the frequency of eutaneous disorders in patients with chronie renal failure presently on hemodialysis(HD). The results were as follows. 1. Cutaneous lesions were present in all 78(100% ) patients with chronic renal failure. 2. The frequency of msjor cutaneous problems associated with chronic renal failure was as follows: xerosis(82.1%), pruritus(74.4%), nail changes(74.4%), hyperpigmentation(70.5 % ), xerostomia(42.3'Yo), poor wound healing(37.2%), easy bruisability(30,8%), hypotrichosis(21.896), and purpura(14.1 % ). 3. Cutaneous problems which appeared after the initiation of HD were. appearance of new pigmented nevi or lentigines(9 patients), appearance or aggravation of acne(6), contact dermatitis at AV fistula site(2), gynecomastia(1), extensive flat warts(1), extensive tinea versicolor(1), and extensive vitiligo(1). 4. Among 55 patients with hyperpigmentation, 11 patients reported decreased pigmentation following HD. In 15 patients, hyperpigmentation worsened following HD, and in 9 patients hyperpigmentation first appeared after HD was initiated. 5. Cutaneous disorders favorably affected by HD were as follows: easy bruisability(3/ 24), xerostomia(3/33), gingival friability(2/7), and hypotrichosis(5/17). 6. Poor wound healing and xerosis were not improved by HD. 7. Among 58 pruritic patients, 7 patients were improved after HD, 7 patients became worse during each HD, and 3 patients were not pruritic only for 2 to 3 days after each HD. Four patients experinced pruritus only during each HD. 8. There was no significant statistical difference between the frequency of pruritus and xerosis and the levels of blood urea nitrogen, calcium, phosphorus, and parathyroid hormone. (Kor J Dermetol 20(3): 313 321,1901)