새로운 스트레스 단백질인 VISP의 세포내 위치

문창훈,윤원준,고명석,김현주,박정우,Moon, Chang-Hoon,Yoon, Won-Joon,Ko, Myoung-Seok,Kim, Hyun-Ju,Park, Jeong-Woo 한국미생물학회 2006 미생물학회지 Vol.42 No.4

이전의 연구 결과 어류 rhabdovirus에 감염된 세포에서 virus-inducible stress protein (VISP)의 발현이 증가함을 확인하였다. 본 연구에서는 VISP의 세포내 위치를 확인하였으며, 또한 세포내 위치 결정에 중요한 역할을 담당하는 VISP의 부위를 확인하였다. 먼저 endogenous VISP의 세포내 위치를 확인하기 위하여 CHSE-214 세포를 VISP에 대한 단클론항체를 사용하여 염색한 후 confocal microscope로 관찰하였다. 그 결과 VISP가 세포의 핵 주변에 점구조를 형성함이 확인되었다. 이를 확인하기 위하여 VISP에 enhanced green fluorescent protein (EGFP)이 붙은 fusion gene을 발현하는 plasmid를 제조하였다. EGFP-VISP를 발현하는 plasmid 벡터를 세포에 transfection 시킨 후 confocal microscope로 관찰한 결과 핵 주변에 점구조를 형성함이 확인되었다. VISP의 아미노산서열 중 핵 주변의 점구조 형성에 관여하는 부분을 확인하기 위하여 VISP의 다양한 deletion mutant들을 제조하였다. 이 mutant를 사용한 transfection 실험 결과 VISP의 C-terminal 부위(aa 612-710)가 핵주변의 점구조 형성에 중요한 역할을 담당함이 확인되었으며, 이 부분의 functional motif 분석결과 691-TLTSLLL-697 부위에 nuclear receptor binding motif가 존재함이 확인되었다. 이와 같은 결과들을 종합하면, VISP는 핵 주변에 존재하며 VISP의 C-terminal부위가 혀 주위 분포에 중요한 역할을 담당함을 알 수 있었다. 이후의 연구로부터 VISP의 핵 주위 분포가 IHNV의 성장에 미치는 영향이 확인되면 IHNV 병원성의 새로운 기작을 밝혀내는 중요한 자료가 될 것이다. Previously we demonstrated that virus-inducible stress protein (VISP) is induced in fish cells by the infection of a fish rhabdovirus. In this paper, we investigated the subcellular localization of the VISP and determined the region of VISP responsible for the subcellular localization. The CHSE-214 cells were stained with monoclonal antibody raised against VISP and observed with confocal microscope to detect the endogenous VISP. The results showed that the VISP localizes to the perinuclear region as spots. A plasmid expressing VISP fused to enhanced green fluorescent protein (EGFP) was constructed. The transient expression of full-length VISP fused to EGFP in CHSE-214 cells confirmed the spot formation of the VISP at perinuclear region. To determine the region responsible for the perinuclear localization of the VISP, we constructed a series of deletion mutants and, by using these deletion mutants, we found that C-terminal region of the VISP (aa 612-710) is essential for the perinuclear distribution of VISP and that this region contained nuclear receptor binding motif (691-TLTSLLL-697). Our results suggest that VISP localizes to the perinuclear region and C-terminal regions are important for this localization. Further studies on the role of the perinuclear localization of VISP in IHNV growth mali reveal the novel mechanism of IHNV pathogenecity.

자가압박금속판을 이용한 전박골 간부골절의 치료

문창훈(Chang Hoon Moon),손치동(Chi Dong Sohn),김용주(Yong Ju Kim) 대한정형외과학회 1986 대한정형외과학회지 Vol.21 No.5

9. 오이의 nitrate reductase activity에 미치는 광과 NO₃의 영향

문창훈(Moon Chang Hoon),정갑채(Chung Gap Chae),하순호(Ha Soon Ho) 한국원예학회 1990 한국원예학회 학술발표요지 Vol.8 No.1

노인 빈혈에 관한 임상적 고찰

문창훈(Chang Hoon Moon),김남성(Nam Sung Kim),김경진(Kyung Jin Kim),석윤철(Yun Chul Suk),권용만(Yong Man Kwon),문창현(Chang Hyun Moon),이인수(In Soo Lee),이중건(Chung Keun Lee) 대한내과학회 1991 대한내과학회지 Vol.40 No.4

N/A Out of 1,500 elderly patients who visited Seoul Adventist Hospital from July, 1988 to 30th June 30, 1989 hed anemia. We have determined the relation of the hemoglobin value and the causative diseases in every patient with anemia. In this study, anemia was defined as follows: less than 6 gm% hemoglobin as severe; greater than or equal to 6 gm% hemoglobin and less than 9 gm% hemoglobin in men, less than 12 gm% in women as mild. The results were as follows: 1) The total incidence of anemia was about 50.5% of the 1500 patients, and those were 55.2% in men, and 45. 4% in women. 2) The of male to female ratio patients with anemia was 1.3:1, and most of them of a were mild degree. 3) The most frequent cause of anemia in these patients was acute infections, Malignancies. chronic infections, GI tract diseases, and hepatobiliary diseases followed that in order. 4) The diseases which accompanied severe degrees of anemia were GI tract diseases with bleding, hematologic disorders, and malignancies. Acute and chronic infections were mainly accompanied by mild anemia. 5) Kidney disease, in which chronic renal failure was the most prominent entity along with malignancies a showed relatively higher percentage of a moderate degree of anemia. 6) Patients with hemorrhage and those with malignancy with distant metastasis showed a relatively lower hemoglobin value than those without them. 7) Among acute infections, infections of the respiratory tract wes the leading cause, and patients with tuberculosis were the majority of those with chronic infections.

연구논문 : 카지노 종사원의 역할갈등이 이직의도에 미치는 영향 -갈등관리전략의 조절효과를 중심으로-

문창훈 ( Chang Hoon Moon ) 한국컨벤션학회 2014 MICE관광연구 Vol.14 No.2

The purpose of this study is to examine preliminary studies on role conflict and turnover intention, the effect of role conflict perceived by casino employees on turnover intention, and the moderating effect of conflict management strategy. First, the direct effect of role conflict on turnover intention was analyzed through multiple regression analysis. To verify this, role conflict was classified into role stress and role ambiguity. The direct effect of role conflict on turnover intention showed that sub-factors of role conflict, both role stress and role ambiguity had a statistically significant effect on turnover intention. One of sub-factors that had the biggest effect on turnover intention was role stress, followed by role ambiguity. Second, the moderating effect of conflict management strategy in the relationship between role conflict and turnover intention showed that the problem-solving type, obliging and avoiding type and control-dominating type moderated the relationship between role ambiguity and turnover intention, the relationship between role stress and turnover intention, and the relationship between role ambiguity and turnover intention, respectively. The problem-solving type of sub-factors of conflict management strategy had a significant effect on turnover intention in the relationship between role conflict and turnover intention. But the obliging and avoiding type and control-dominating type didn``t moderate in the relationship between role conflict and turnover intention.

오이(Cucumis sativus L.)의 窒酸環元 酵素의 活性에 미치는 光과 NO₃<SUP>-</SUP>의 影響

文彰勳(Moon Chang Hoon),鄭甲彩(Chung Gap Chae),河淳鎬(Ha Soon Ho) 한국원예학회 1991 Horticulture, Environment, and Biotechnology Vol.32 No.2

혈액투석치료를 받은 외과적 급성 신부전 환자의 임상 양상과 예후인자

박훈기(Hoon Ki Park),김동진(Dong Jin Kim),박경식(Kyoung Sik Park),강성원(Sung Won Kang),문창훈(Chang Hoon Moon),이규백(Kyu Baik Lee),김향(Hyang Kim),구자룡(Ja Ryong Koo),이영호(Young Ho Lee),장미경(Mi Kyoung Chang),권영주(Young Ju K 대한내과학회 1996 대한내과학회지 Vol.50 No.4

N/A Objectives: Acute renal failure continues to be a significant cause of postoperative morbidity and mortality, especially after operations or trauma. Post- operative renal failure carries a mortality rate over 50%. We had two goals-to identify preoperative and intraoperative risk facors for postoperative acute ranal failure in patients groups undergoing various types of surgery, and by the use of multivariate analysis, to assess the relative important of these risk factors to provide a means of estimating the probability of survival in an individual patient. Methods: Data review was carried out for the 68 adult patients with ARF treated at 6 hospitals over a 3-year period(January 1991-December 1993). These patients were surgical patients who developed ARF in the postoperative period and subsequently underwent hemodialysis. Thirty-five factors to be of potential relevance to the outcome in postoperative acute renal failure were tabulated and analyzed. Results: 1) The ratio of male to female was 2:1, and the mean age was 51±17 years. The prevalence reached peak over 60 years. The mean death rate, numbers of hemodialysis, duration of oligura, numbers of organ failed, interval from ARF to recovery and interval from ARF to death were 63%, 9±0.8, 11±1 days, 1.7±0.2, 28±2 days, 19±3 days respectively. 2) The complications which developed during ARF included pulmonary complication(57%), hypotension (51%) and sepsis(32%), etc. 3) In findings related to operation, the mean duration of operation, interval from operation to ARF, numbers of transfusion during operation, frequency of hypatension during operation and duration of hypotension during operation were 211±126 min, 3.5±4 days, 23±3pints, 55%, 39.95±8.84 min respectively. 4) In the univariate analysis, hypotension, pulmonary complication, numbers of organ faild, pH (pH<7.2 or pH>7.55), APACHE II score, sepsis, duration of operation and duration of hypotension during operation were the only factors among 35 risk factors that significantly correlated with post- operative acute renal failure. In the multivariate analysis, the only significant risk factors for postoperative renal failure were hypotension and the presence of underlying disease. Conclusion: From the above results. The physician caring for the postoperative ARF patient may depend on these risk factors to predict morbidity and mortality. Whether or not these factors help to prevent the severe morbidity and mortality associated with postoperative renal failure remains to be studied prospectively.

연구논문(硏究論文) : 카지노 종사원의 고객혼잡지각이 직무스트레스에 미치는 영향 -경력몰입의 조절효과를 중심으로-

허윤미 ( Yoon Mi Heo ),문창훈 ( Chang Hoon Moon ) 한국관광산업학회 2016 Tourism Research Vol.41 No.1

본 연구에서는 고객의 서비스 평가에 있어서 중요한 역할을 담당하는 종사원을 대상으로 하여 고객혼잡상황이 종사원의 스트레스에 어떠한 영향을 미치는지 살펴보고자 한다. 더불어, 두 변수 간의 관계에 있어서 종사원의 개인적 특성인 경력몰입을 조절변수로 하여 연구모형을 설정하였다. 본 연구의 결과는 다음과 같다. 첫 번째, 종사원들의 고객혼잡지각과 직무스트레스 간의 관계를 검증한 결과 종사원의 지각된 인적ㆍ공간적 혼잡과 직무스트레스관계에서 종사원의 혼잡지각정도가 높을수록 직무스트레스가 증가하는 것으로 나타났다. 두 번째, 고객 혼잡지각에 따른 직무스트레스의 관계에서 경력몰입의 조절효과를 검증하였다. 그 결과, 종사원에게 지각되는 혼잡은 직무스트레스와의 관계에서 경력몰입이 조절효과 검증결과와는 별개로 직무스트레스를 감소시켜 다른 선행연구의 결과와 일치하는 것으로 이해된다. 본 연구는 연구자들과 카지노 기업을 포함하는 서비스 기업의 관리자들에게 고객혼잡상황과 종사원들의 영향변수 사이의 관계를 보다 잘 이해할 수 있는 결과 및 향후 연구방향을 제시하고 있으며, 실무적으로는 혼잡지각을 상쇄할 수 있는 서비스 환경의 조성 및 종사원 교육의 필요성을 제시하고 있다. This study would exanine how the customer-crowded-situation has influence on stresses of employees who perform important roles in getting eoaluation for the quality customer service. Furthermore, in studying the relationship between two uaroables, we established a research model by setting the career commitment as the moderating uariable that is indiuidual characteristic of employees. The results of this research are as follow. First, as 10e tested the relationship between the perception of customer-crowded-situation and job stresses of employees, it showed that more the employees aware the humane □ spacial chaotic, more stresses they tend to get. Second, we tested a control effect of career commitment in the relationship between the perception of customer-crowded-situation and fob stresses. As a results it turned out that career commitment does not giue meaningful influence on stresses. This shows the same results with other preceding researches on reducing fob stresses separately {pith the test results of results of control effect of career commitment. This research suggests ways for researchers and company administrators in the sen1ice industry including Casino industry to understand better the relationship between the customer-crowded-situation and influence variables of employees and to suggest the .future direction of research. 1t also proposes the necessity of fostering a service environment and providing employee training that can offset the perception of customer-crowded-situation.

연세 중앙교회의 구조설계

남기춘(Kee Choon Nam),문창훈(Chang Hoon Moon),김형운(Hyoung Woon Kim),이수권(Soo Kueon Lee) 한국강구조학회 2005 韓國鋼構造學會誌 Vol.17 No.3

위장관 ( 胃腸管 ) : 비만 ( 肥滿 ) 음주 ( 飮酒 ) 흡연등 ( 吸煙等 ) 제요소 ( 諸要素 ) 와 식도 ( 食道 ) 열공 ( 裂孔 ) Hernia 빈도 ( 頻度 ) 에 대한 고찰

김경진(Kyung Jin Kim),이재동(Jae Dong Lee),석윤철(Yoon Chul Suk),문창훈(Chang Hoon Moon),김선민(Sun Min Kim),마상인(Sang In Ma),박경식(Kyung Sik Park),이중건(Choong Kun Lee) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.4

N/A Clinical studies were carried out on 581 cases who had taken gastrofiberscopic examination because of upper gastrointestinal syptms from July, 1989 to April, 1990 in Seoul Adventist Hospital. The result about esophageal hiatal hernia were as follows; 1) Of total 581 cases, 310 were male, and 271 were female. Of total 581 cases, 73 were averweight group, 207 were drinkmg group, and 18~8 emoki~ng group. 2) Of total 58I cases, 48 were esophageal hiatal hernia group. The incidence of hernia was 8.2%. Of 48 hernia casea, 38 were male, and 10 were female. Of 48 hernia cases, 30 were over aged 40, and 18 were under 40. 3) In overweight group, 19 were hernia cases (26%), and non-overweight group, 29 were hernia cases (5.7%) 4) In drinking group, 30 were hernia caees (14.5%), and non-dringking group, 18 were hernia cases (4.8%). 5) In smoking group, 27 were hernia cases (14.4%) and in non-smoking group, 21 were hernia cases (5.3%) 6) In conclusion, in overweight, drinking, and smoking group, the incidence of esophageal hiatal hernia were significantly higher than non-overweight, non-drinking, and non-smoking group, respectively.