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문소은,석은지,하진아,양현원,윤보경,이민주 한국발생생물학회 2024 발생과 생식 Vol.28 No.1
Gonadotropin-releasing hormone (GnRH), a critical hormone produced in the hypothalamus, is essential for regulating reproductive processes. It has also been demonstrated the presence of GnRH and its receptors (GnRHR) in ovarian and uterine tissues, but little was known about the regulation mechanism of their expression in these organs and ovarian aging. Therefore, the aim of this study was to investigate the expression of GnRHR in the ovary and uterus of mice, particularly after high-dose gonadotropin treatments and in relation to aging. Quantitative realtime-PCR (qRT-PCR) revealed that pituitary gland had the highest GnRHR expression in both young and aged mice. In addition, liver expression was higher in young mice, whereas thymus expression was higher in aged mice. GnRHR mRNA was present in the ovaries of both young and aged mice but nearly undetectable in the uterus of aged mice. We next examined the expression of GnRHR in the ovary and uterus in response to high-dose administration of pregnant mare serum gonadotropin (PMSG). After PMSG administration, GnRH mRNA levels were significantly decreased in the ovary but increased in the uterus. The expression of GnRH mRNA in these organs showed opposite trends to that of GnRHR expression. These results suggest the involvement of GnRH in age-related reproductive decline and the potential effects of high-dose gonadotropin treatments on reproductive organ function.
문소은,이창구,신귀암,박성직 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.12
This study focuses on the adsorption of hexavalent chromium (Cr(VI)), a harmful heavy metal, using two types of chlorella-based biochar (biochar pyrolyzed at 200 °C (CB200) and iron chloride (FeCl3)-modified biochar pyrolyzed at 200 °C (FeCB200)), and the testing of their effectiveness for the removal of Cr(VI). The FeCB200 sample exhibited the highest removal efficiency (1 g L−1=66.12±1.01%; 5 g L−1=98.48±0.22%) compared to raw biomass (1 g L−1=54.05±0.00%; 5 g L−1=90.09±0.26%) and CB200 (1 g L−1=51.80±0.78%; 5 g L−1=94.74±0.26%) in 100 mg L−1 Cr(VI) solution. The adsorbents were characterized using various characterization techniques, and adsorption experiments were carried out using varying doses of the adsorbent (1 and 5 g L−1). Pseudo-second-order model provided the best fit for the adsorption kinetics, and Redlich-Peterson model exhibited good fitting for the adsorption isotherm (R2>0.972), although variations were observed depending on the dose. Further, the applicability of FeCB200 was assessed using real wastewater spiked with Cr(VI). Although the presence of organic matter resulted in a reduction in the adsorption effectiveness of FeCB200, the difference was not significant (1 g L−1=54.27±3.19%; 5 g L−1=98.48±0.22%). These results demonstrate the promising potential of FeCl3-modified chlorella-based biochar as a valuable adsorbent for the Cr(VI) removal from water environments.