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이승현,이창현,김홍준,김영식,신민지,김지현,마상용,이세연,권진,오찬호,Lee, Seung-Hyeon,Lee, Chang-Hyun,Kim, Hong-Jun,Kim, Young-Sik,Shin, Min-Ji,Kim, Ji-Hyun,Ma, Sang-Yong,Lee, Se-Youn,Kwon, Jin,Oh, Chan-Ho 대한한의학방제학회 2021 大韓韓醫學方劑學會誌 Vol.29 No.2
Objectives : This study was conducted to evaluate the efficacy of a complex mixture of natural substances of ginseng and baeknyeoncho on the arthritic rats. Methods : In vitro experiments were conducted to ensure the stability of the complex. After setting toxicity and concentration by MTT assay, the antioxidant effect was measured through DPPH and ABTS radical scavenging activity. To confirm the anti-inflammatory effects of the complex, levels of nitric oxide (NO) and pro-inflammatory cytokines (IL-1β, TNF-α) were measured in LPS-treated macrophage cell lines (RAW264.7). We injected monosodium iodoacetate (MIA) 50 μl (60 mg/ml) into knee joints of rats to induce osteoarthritis. The rats were divided into three groups (normal (n=5), control (n=5), and OR (n=5) group). The control group consumed 2 mg/kg of physiological saline once a day for 4 weeks, and the OR group was mixed at a concentration of 416.5 mg/kg of Baengnyeoncho (O) and 208.25 mg/kg of red ginseng (R) and ingested 1 mL each 5 days a week. Results : This complex increased the DPPH and ABTS radical scavenging rate. The complex decreased NO production and pro-inflammatory cytokine production of macrophages. In the OR group, the secretion of cytokine in serum was decreased. In histopathological examination, the joint tissue of the composite showed less damage to the synovial membrane, cartilage, and fibrous tissue than the control group. Conclusions : As a result of this study, natural complexes have antioxidant, anti-inflammatory and cartilage protection effects. Therefore, we expect the complex to be effective in treating osteoarthritis.
식물체 및 토양중에 있어서 제초제(除草劑) Pretilachlor 의 이동특성
문영희,마상용,양환승 한국농화학회 1987 Applied Biological Chemistry (Appl Biol Chem) Vol.30 No.4
This study was conducted to investigate the absorption and translocation of pretilachlor [2-chloro-2, 6-diethyl-N-(n-propoxyethyl)-acetanilide] in plants and to evaluate the mobility in soil using the ^(14)C-or non-labelled compound in laboratory. Rice plant(Oryza sativa L.) was very tolerant to pretilachlor. Echinochloa crus-galli P. Beauv. was completely con trolled by pretilachlor at 60g a.i./10a. At the 120g a.i./l0a, growth of Cyperus serotinus Rottb. and Sagittaria pygmaea Miq. was inhibited by 75% and 25%, respectively. The growth inhibition depended on absorbed amount of ^(14)C pretilachlor. The greatest concentration of ^(14)C was found in E. crus-galli, whereas the lowest was determined in rice plant. The rate of absorption and translocation in E. curs-galli was faster than in rice plan. Pretilachlor moved to 6㎝ deep in sandy clay loam, clay loam and loam soils, but to l0㎝ in sandy loam soil. In the soils herbicide-treated layer was found 0 to 2㎝ profile.
토양중에 있어서 제초제(除草劑) Pretilachlor 의 분해성에 미치는 몇가지 토양특성의 영향
문영희,마상용,양환승,장익선 한국농화학회 1988 Applied Biological Chemistry (Appl Biol Chem) Vol.31 No.2
This study was conducted to evaluate effect of soil texture, organic matter, temperature and water regime on degradation of pretilachlor (2-chloro-2', 6'-diethyl-N-(n-propoxyethyl) acetanilide) in the soils. The period of residual activity in soil treated with pretilachlor at rate of 60g a.i./10a was about 50 days. Also, period of reaching GR 50 value on the barnyard grass was about 25∼27 days. Degradation rate of pretilachlor in the sandy clay loam soil which has higher contents of organic matter and clay was faster than in the sandy loam soil. When organic matter was added to the soil, the rate of decomposition was accelerated. The faster degradation occurred under the 30℃ soil temperature by comparison under the 20℃. The rate of degradation in the soil was faster under the flooded conditions than under the wetted conditions.