만성 알코올 섭취시 2 - Acetylaminofluorene 투여가 흰쥐 간 미토콘드리아 ATPase 활성도와 막지질 조성에 미치는 영향

류선영(Sun-Young Ryu),김정희(Jung-Hee Kim) 한국식품영양과학회 1995 한국식품영양과학회지 Vol.24 No.6

만성적인 알코올 섭취가 미토콘드리아의 지질조성 및 ATPase 활성도에 미치는 영향을 조사하고 아울러 간의 발암물질인 2-AAF를 투여하여 간 미토콘드리아의 기능에 미치는 상호 효과를 조사하기 위하여 120~125g 숫쥐에게 열량의 35%를 알코올로 공급하여 6주간 사육하였으며 알코올 섭취 4주 후에 2-AAF를 3일 간격으로 2회 복강투여하여 실험한 결과를 요약하면 다음과 같다. 1. 체중 증가, 식이 섭취량 및 식이효율에 대한 알코올의 효과는 유의적인 차이를 보였으나, 2-AAF에 의한 유의적인 효과는 관찰할 수 없었다. 알코올 섭취군이 대조군 보다 체중은 유의적으로 감소하였다. 2. 미토콘드리아 ATPase 활성도는 알코올 섭취에 의해 유의적으로 감소되었고, 2-AAF 투여에 의해서는 유의적이지는 않으나 감소되는 경향을 보였다. 따라서 대조군(C)에 비하여 알코올 섭취와 2-AAF투여군 (EA)에서 ATPase 활성도가 29.3%의 감소를 나타냈다. 3. 간 미토콘드리아 콜레스테롤 함량은 알코올 섭취에 의해 유의적으로 증가하였으며 미토콘드리아 총 인지질량은 알코올과 2-AAF에 의한 상호작용에 의해 알코올 투여군에서만 2-AAF 투여시 유의적인 증가 효과를 보여주었다. C/PL 비율은 알코올 섭취와 2-AAF 투여 모두에서 유의적인 효과는 보이지 않았으나 군간 비교시 알코올군(E)이 대조군(C)보다 유의적으로 증가하였다. 4. 간 미토콘드리아의 CL량은 알코올군에서 감소하는 경향을 보였고 PS와 PI량은 차이가 없었다. 알코올 섭취로 PC량은 유의적으로 증가하였으나 PE량은 차이가 없었으며, 2-AAF 투여서 PC량은 유의적인 감소를 보였고 PE량은 유의적으로 증가하였다. PE/PC 비율은 알코올 투여에 의해 유의성은 없었으나 감소경향을 나타냈으며, 2-AAF 투여에 의해서는 유의적인 증가 효과가 나타났다. 5. 알코올 섭취시 미토콘드리아의 지방산 조성은 palmitic acid가 유의적으로 감소하였고, stearic acid는 유의적으로 증가함을 보였다. UFA/SFA와 UI는 알코올군에서 유의적이지는 않으나 증가되는 경향을 보였다. 2-AAF 투여에 의해서도 linoleic acid는 감소되고 arachidonic acid는 증가되는 경향을 보여 UI값이 증가되는 경향이 있었다. 따라서 본 실험에서 나타난 ATPase 활성도 변화는 미토콘드리아의 콜레스테롤변화, C/PL 비율, 인지질 조성이나 지방산 조성의 변화 등 막지질 조성의 변화에 기인하는 것으로 생각된다. This study was done to investigate the effect of chronic alcohol feeding and acetylaminofluorene (2-AAF) treatment on hepatic mitochondrial ATPase activity and membrane lipid composition. Male Sprague-Dawley rats, weighing 120~125g, were fed for 6 weeks on a liquid diet containing 35% of calories as ethanol. After 4 weeks of experimental diet feeding, 2-AAF (100㎎/㎏ body weight) was injected twice a week intraperitoneally. Body weight and percent liver weight per body weight were significantly changed by ethanol feeding. Hepatic mitochondrial ATPase activity significantly decreased by ethanol feeding but not by 2-AAF treatment. In comparison to control, the ATPase activity of ethanol-AAF group decreased 29.3%. Since phospholipid(PL) content of mitochondria has an interaction effect between ethanol and 2-AAF treatment, 2-AAF treatment significantly increased phospholipid content in only ethanol fed group. Total cholesterol(C) level of mitochondria significantly increased by ethanol feeding. Consequently C/PL ratio of ethanol group was significantly higher than that of control group. The analysis of mitochondrial PL composition showed that cardiolipin(CL) significantly increased by 2-AAF treatment in control group. Phosphatidyl choline (PC) significantly increased by ethanol feeding, whereas PC significantly decreased and phosphatidyl ethanolamine (PE) significantly increased by 2-AAF treatment. 2-AAF treatment also showed a significant increase in PE/PC ratio. Fatty acid patterns of mitochondria were also changed by either ethanol or 2-AAF although the severity of the changes was not great. These data suggest that the reduced mitochondrial ATPase activity in ethanol-AAF group may be a consequence of a changes in mitochondrial membrane lipid composition such as PE/PC ratio, C/PL ratio and fatty acid patterns.

당뇨병성 위마비 환자에 대해 한방치료 후 위 운동성 및 혈당조절이 호전된 2예 보고

장선영,엄국현,이선령,김현경,이준석,유종민,김진성,류봉하,류기원,류재환,윤상협,Jang, Sun-Young,Eom, Guk-Hyeon,Lee, Seon-Young,Kim, Hyun-Kyung,Lee, Joon-Suk,Ryu, Jong-Min,Kim, Jin-Sung,Ryu, Bong-Ha,Ryu, Ki-Won,Lew, Jae-Hwan,Yoon, Sang-H 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.1

Diabetic gastroparesis is a disorder in a diabetic of delayed gastric emptying in the absence of mechanical obstruction. It is estimated that about 20%-50% of diabetics suffer from gastroparesis. Clinical Sympoms include early satiety, bloating, nausea, anorexia, vomiting, abdominal pain, and weight loss. Severe gastroparesis might result in recurrent hospitalization, malnutrition, and even death, but, no exact treatment has yet been established. Electrogastrography has been known to be a simple, non-invasive, and effective method in assessing gastric motility and EGG has been used to diagnosis diabetic gastroparesis and to estimate its prognosis. Recently, two cases of diabetic gastroparesis were observed. For this cases, manual acupucture, electroacupucture, lumbar skin warming and herb medicine were applied. After application of these therapies, gastrointestinal symptoms improved and these therapeutic effects were confirmed in EGG. Moreover, blood glucose control improved, so patients were able to discontinue insulin injection and change to p.o.medication. In light of this encouraging application of oriental medicine, this is reported along with investigation of the literature.

비위관 급식환자에서의 설사 치험 2례

임중화(Joong-Hwa Lim),유종민(Jong-Min Ryu),장선영(Sun-Young Jang),한숙영(Sook-Young Han),박석규(Seok-Kyu Park),홍상선(Sang-Sun Hong),김진성(Jin-Sung Kim),윤상협(Sang-Hyub Yoon),류봉하(Bong-Ha Ryu),류기원(Ki-Won Ryu) 대한한의학회 2003 대한한의학회지 Vol.24 No.3

구강함수제 개발을 위한 오매, 비파엽, 오가피, 백지의 구취억제효과 연구

장선영(Sun-Young Jang),박재우(Jae-Woo Park),윤성우(Seong-Woo Yoon),류봉하(Bong-Ha Ryu),김진성(Jinsung Kim) 대한한의학회 2010 대한한의학회지 Vol.31 No.4

18F-FDG PET/CT에서 우연히 관찰되는 구개편도의 비대칭적 섭취증가의 임상적 의의

채선영 ( Sun Young Chae ),백소라 ( So Ra Baek ),류진숙 ( Jin Sook Ryu ),문대혁 ( Dae Hyuk Moon ),김재승 ( Jae Seung Kim ) 대한핵의학회 2009 핵의학 분자영상 Vol.43 No.5

목적: 이 연구는 다양한 임상 적응증으로 시행된 전신 18F-FDG PET/CT 영상에서 구개편도의 비대칭적인 섭취증가의 발견빈도와 악성여부를 조사하고, 악성병변을 시사하는 임상 및 PET/CT 영상소견들을 알아보고자 하였다. 대상 및 방법: 1년 동안 18F-FDG PET/CT를 시행한 2,901명(평균연령 58.4±12.3세, 연령범위 20~88세, 남성:여성=1,841:1,060)의 PET/CT영상에서 구개편도의 비대칭적 섭취증가 여부 및 형태를 육안적으로 분석하였다. 구개편도에 비대칭적인 섭취증가를 보이는 빈도와 악성률을 조사하였고 관련된 임상적 소견 및 PET/CT 영상에서 동반된 이상소견과의 연관성을 평가하였다. 결과: 2,901명 중 10.0%(291명)에서 구개편도에 비대칭적인 섭취증가를 보였으며 겨울에 가장 빈번하게 관찰되었다(12.1%). 임상 추적관찰 혹은 조직학적 검사를 통해 최종적으로 악성여부가 확인된 209명 중 2.4%(5명)가 악성병변으로 진단되었고 불규칙한 두개편도의 섭취증가나 경부림프절의 섭취증가 소견이 동반된 경우, 원발미상의 경부림프절전이 환자에서 PET/CT를 시행한 경우 악성률이 높았다. 결론: 전신 18F-FDG PET/CT에서 우연히 관찰되는 구개편도의 비대칭적인 섭취증가는 대부분이 양성으로 악성률은 낮았다. 그러나 원발미상의 경부림프절 전이환자나 PET/CT에서 구개편도의 섭취증가가 불규칙할 경우에는 악성의 가능성이 높기 때문에 적극적인 조직학적 검사가 필요하리라 생각된다. Purpose: The aim of this study was to determine the incidence and malignant rate of incidental asymmetric palatine tonsillar uptake (ATU) on 18F-FDG PET/CT in various clinical indications and to evaluate the clinical and PET/CT findings suggesting malignancy. Materials and Methods: We retrospectively reviewed a total of 2,901 patients (58.4±12.3 yrs, range 20~88 yrs, M:F=1,841:1,060) who underwent 18F-FDG PET/CT during an 1-year period with various indications except primary tonsillar cancer and lymphoma evaluation. On 18F-FDG PET/CT, metabolic abnormality of the palatine tonsil and cervical lymph node were visually assessed. ATU was defined as increased palatine tonsillar uptake with diffuse, focal, or irregular pattern compared to contralateral side. The incidence and malignant ratio of ATU were evaluated according to clinical and PET/CT findings. Results: Of 2,901 cases, 290 (10.0%) showed ATU. The incidence of ATU showed seasonal variation and was high in the winter (12.1%). Of 209 cases with ATU confirmed pathologically and/or clinically, five (2.4%) were malignant lesions. ATU with irregular uptake pattern (2/2) and in cases referred for cervical lymph node metastasis of unknown origin (3/5) were frequently associated with malignant lesion (p<0.05). Conclusion: ATU was not infrequently observed on 18F-FDG PET/CT, and the malignant risk of ATU was low. However, ATU with cervical lymph node metastasis or with irregular pattern on PET/CT would be further evaluated by the histopathologic examination. (Nucl Med Mol Imaging 2009;43(5):402-410)

사이버 공격예측 및 대응을 위한 유연적 시험환경 구축

류한얼(Han-Eul Ryu),전성구(Sung-Goo Jun),심신우(Shin-Woo Shim),임선영(Sun-Young Im),한인성(In-Sung Han),오행록(Haeng-Rok Oh) 한국통신학회 2019 韓國通信學會論文誌 Vol.44 No.9

한방치료가 기능성 소화불량증 환자의 위전도 소견에 미치는 효과

유종민,장선영,김현경,이준석,엄국현,이선령,김진성,류봉하,류기원,윤상협,Ryu, Jong-Min,Jang, Sun-Young,Kim, Hyun-Kyung,Lee, Joon-Suk,Eom, Guk-Hyeon,Lee, Seon-Young,Kim, Jin-Sung,Ryu, Bong-Ha,Ryu, Ki-Won,Yoon, Sang-Hyub 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

Background & Object: The aim of this study was to investigate the effects of Traditional Korean medical therapy on gastric myoelectrical activity in patients with functional dyspepsia. Methods: 29 patients(male 10, female 19) were divided into two groups; Inpatient group(N=10, male 1, female 9) and outpatient group(N=19, male 9, female 10). 10 healthy people participated in this study as normal controls. Gastric motility was recorded and analyzed by electrogastrography(EGG) during fasting and postprandial periods. Hospital treatment included electroacupuncture on ST36, acupuncture, and herbal medicine. Outpatients were treated with herbal medicine and acupuncture. Inpatients underwent hospital treatment for two weeks, and after discharge they were treated as outpatients for six weeks. In inpatient group, EGG was recorded before and after hospital treatment and it was recorded at six weeks after discharge. In outpatient group, EGG was recorded before starting treatment and it was recorded once a every six weeks for twelve weeks. Results: There was no significant difference in EGG parameters between before and after therapy in outpatient group. In inpatient group, postprandial normal slow wave increased after hospital treatment(from $33.94{\pm}14.13$ to $62.63{\pm}20.18$, p<0.05) and postprandial tachygastria decreased(from $43.53{\pm}13.99$ to $28.30{\pm}16.63$, p<0.05) and arrhythmia decreased(from $20.35{\pm}15.91$ to $5.67{\pm}6.80$, p<0.05). Conclusions: Results suggest that hospital treatment through electroacupuncture on ST36, acupuncture, and herbal medicine is useful to improve gastric myoelectrical activity in Patients with Functional Dyspepsia.

Ca Cell의 보호막으로 증착된 (SiO₂)_1-x(ZnO)_x 무기 혼합 박막들의 투습 특성

김화민,류성원,손선영,Kim, Hwa-Min,Ryu, Sung-Won,Sohn, Sun-Young 한국전기전자재료학회 2009 전기전자재료학회논문지 Vol.22 No.3

We investigated the properties of inorganic diatomic films like silicon oxide ($SiO_2$) and zinc oxide (ZnO) and their composite films are packed as a passivation layer around Ca cells on glass substrates by using an electron-beam evaporation technique and rf-magnetron sputtering method. When these Ca cells are exposed to an ambient atmosphere, the water vapor penetrating through the passivation layers is adsorbed in the Ca cells, resulting in a gradual progress of transparency in the Ca cells, which can be represented by changes of the optical transmittance in the visible range. Compared with the saturation times for the Ca cells to become completely transparent in the atmosphere, the protection effects against permeation of water vapor are estimated for various passivation films. The thin composite films consist of$SiO_2$ and ZnO are found to show a superior protection effect from water vapor permeation compared with diatomic inorganic films like $SiO_2$ and ZnO. Also, this inorganic thin composite films are also found that their protection effect against permeation of water vapor can be significantly enhanced by choosing their suitable composition ratio and deposition method, in addition, the main factors affecting the permeation of water vapor through the oxide films are found to be the polarizability and the packing density.

기능성 소화불량증 환자의 피로도에 대한 체표 교감신경 활성 및 심혈관 반응의 연계성 (양도락과 맥진검사의 진단적 가치)

김현경,유종민,장선영,이준석,엄국현,이선령,김진성,류봉하,류기원,윤상협,Kim, Hyun-Kyung,Ryu, Jong-Min,Jang, Sun-Young,Lee, Joon-Suk,Eom, Guk-Hyeon,Lee, Seon-Young,Kim, Jin-Sung,Ryu, Bong-Ha,Ryu, Ki-Won,Yoon, Sang-Hyub 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.2

Background & Object: The aim of this study was to investigate the association of degree of fatigue and gastric motility, measured by EGG, with skin sympathetic tone or cardiovascular reactivity in patients with functional dyspepsia. Methods: Subjects were 56 patients with Functional dyspepsia and eight healthy people. Degree of fatigue was assessed by questionnaires consisting of subjective complaints of fatigue. Skin sympathetic tone was measured by Ryodoraku Score and Cardiovascular Reactivity was checked by Pulse diagnostic apparatus. Gastric motility was estimated by EGG. First, all patients were divided into two groups by Ryodoraku Score $40{\mu}A$(below and above). Second, they were subdivided into two groups by Cardiovascular Reactivity(decreased and increased or not decreased). Estimates were made on the extent differences of degree of fatigue or state of gastric motility in each group. Results: 1. Fatigue scores was significantly higher in females and in the Ryodoraku-Score-below-$40{\mu}A$ group. It was higher in the decreased cardiovascular reactivity group than the increased group, but to no significanct extent. Also, gastric motility was better in the Ryodoraku-Score-above-$40{\mu}A$, group than in the below group. Conclusions: These results suggest that degree of fatigue and gastric motility are associated with skin sympathetic tone, but not associated with cardiovascular reactivity, and that $40{\mu}A$ is a useful cutoff point in Ryodoraku Score for assessing degree of fatigue in functional dyspepsia patients.

한방병원에 내원한 위마비 및 비위마비성 기능성 소화불량증 환자에 대한 위 운동성 및 임상적 속성에 관한 연구

이준석,이선령,엄국현,김현경,장선영,유종민,김진성,류봉하,류기원,윤상협,Lee, Joon-Suk,Lee, Seon-Young,Eom, Guk-Hyeon,Kim, Hyun-Kyung,Jang, Sun-Young,Ryu, Jong-Min,Kim, Jin-Sung,Ryu, Bong-Ha,Ryu, Ki-Won,Yoon, Sang-Hyub 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.4

Little is known concerning the pathophysiology and symptomatology of gastric dysmotility of patients who have sought treatment in eastern traditional medicine. The aim of this study was to investigate gastric electrical activity and clinical characteristics between gastroparesis and non-gastroparetic functional dyspepsia(FD) and assess the necessity to distinguish them for eastern traditional medical treatment. 60 patients were surveyed by questionnaire. Gastric electrical activity was recorded and analyzed using electrogas-trography. Patients were divided into gastroparesis and non-gastroparetic FD by 1.0 of power ratio. Healthy persons were used as reference. Postprandial normogastria of gastroparesis decreased and that of non-gastroparetic functional dyspepsia increased compared before and after meals. Suggested causes of onset were the meat diets that most patients were on in the gastroparesis group, and that drugs that patients in the non-gastroparetic FD group were taking. Distribution of main symptom was equally regular in patients with gastroparesis, but patients with non-gastroparetic FD showed mainly upper abdominal pain and fullness. Values for the gastroparesis group were double those for the non-gastroparesis FD group in frequency of upper GI endosopic examination. Results suggest that effective application of eastern traditional medical treatment requires that first a clear distinction be made in state of gastric dysmotility and clinical characteristics of gastroparesis and non-gastroparesis FD when treating dyspepsia.