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김정심,노수정,남영준,Kim, Jeong-Sim,Roh, Soo-Jeong,Nam, Young-Joon 한국과학기술정보연구원 과학기술정보센터 2010 Journal of Information Science Theory and Practice Vol.41 No.1
국가행정박물은 일반박물과 같은 물리적 특징을 갖고 있으며, 공공기관이 업무 수행 과정 중에 생산한 것으로 증거적 가치를 지니고 있다. 국가행정박물은 일반 박물 및 문화재와는 내용적으로 구분이 되며, 일반 종이기록물 및 전자기록물과는 그 물리적 성질이 다르므로 관리 프로세스를 정교하게 확장하여야 한다. 이에 이 연구에서는 우리나라 기록물관리법을 근거로 ISAD(G), MODS, RAD, CDWA의 기술요소에서 관련요소를 비교 추출하여 국가 행정박물의 확장된 기술요소를 도출하였다. National artifacts has the same physical characteristics to general artifacts and the evidential value which was produced during the public working. National artifacts different from paper and electronic records about physical properties. So it should be doing the expanded process. In this study, the ISAD(G), MODS, RAD and CDWA based on Korean Record Management Law by comparing and analysing of the technical elements was being extracted, The descriptive elements of national artifacts is pull by conclusion of the analysis.
서명지,이지연,남영도,이수영,박소림,이성훈,이미화,노성운,최학종,임성일 한국산업식품공학회 2013 산업 식품공학 Vol.17 No.4
The Lactobacillus brevis 340G strain isolated from traditional Korean fermented food (kimchi) produced 15.50 mM of γ-aminobutyric acid (GABA) after 48 h of cultivation in MRS media containing 1% L-monosodium glutamate (MSG). The culture conditions of Lb. brevis 340G were optimized for GABA production. Lb. brevis 340G was cultivated at 30oC in optimized MRS media containing 3% sucrose and 2% yeast extract with 3% MSG, resulting in maximum GABA production (68.77 mM) after 54 h of cultivation. Skim milk fermented with Lb. brevis 340G produced 4.64 mM of GABA in the presence of 1% MSG. These results suggest that Lb. brevis 340G could be used as a starter for functional fermented foods and skim milk fermented with Lb. brevis 340G could be further developed to become functional dairy food fortified with GABA.
석윤지,송은지,차인태,이현진,노성운,정지영,이유경,남영도,서명지 한국미생물·생명공학회 2016 한국미생물·생명공학회지 Vol.44 No.2
Recent succession of soil microorganisms and vegetation has occurred in the glacier foreland, because of glacier thawing. In this study, whole microbial communities, including bacteria, archaea, and eukaryotes, from the glacier foreland of Midtre Lovénbreen in Svalbard were analyzed by metagenome sequencing, using the Ion Torrent Personal Genome Machine (PGM) platform. Soil samples were collected from two research sites (ML4 and ML7), with different exposure times, from the ice. A total of 2,798,108 and 1,691,859 reads were utilized for microbial community analysis based on the metagenomic sequences of ML4 and ML7, respectively. The relative abundance of microbial communities at the domain level showed a high proportion of bacteria (about 86−87%), whereas archaeal and eukaryotic communities were poorly represented by less than 1%. The remaining 12% of the sequences were found to be unclassified. Predominant bacterial groups included Proteobacteria (40.3% from ML4 and 43.3% from ML7) and Actinobacteria (22.9% and 24.9%). Major groups of Archaea included Euryarchaeota (84.4% and 81.1%), followed by Crenarchaeota (10.6% and 13.1%). In the case of eukaryotes, both ML4 and ML7 samples showed Ascomycota (33.8% and 45.0%) as the major group. These findings suggest that metagenome analysis using the Ion Torrent PGM platform could be suitably applied to analyze whole microbial community structures, providing a basis for assessing the relative importance of predominant groups of bacterial, archaeal, and eukaryotic microbial communities in the Arctic glacier foreland of Midtre Lovénbreen, with high resolution.
조의상,조인태,최학종,노성운,남영도,서성만,서명지 한국미생물학회 2018 The journal of microbiology Vol.56 No.12
A yellow pigmented bacterium designated strain MBLN094T within the family Flavobacteriaceae was isolated from a halophyte Salicornia europaea on the coast of the Yellow Sea. This strain was a Gram-stain negative, aerobic, non-spore forming, rod-shaped bacterium. Phylogenetic analysis of the 16S rRNA gene sequence of strain MBLN094T was found to be related to the genus Zunongwangia, exhibiting 16S rRNA gene sequence similarity values of 97.0, 96.8, 96.4, and 96.3% to Zunongwangia mangrovi P2E16T, Z. profunda SM-A87T, Z. atlantica 22II14-10F7T, and Z. endophytica CPA58T, respectively. Strain MBLN094T grew at 20‒37°C (optimum, 25‒ 30°C), at pH 6.0‒10.0 (optimum, 7.0‒8.0), and with 0.5‒15.0% (w/v) NaCl (optimum, 2.0‒5.0%). Menaquinone MK-6 was the sole respiratory quinone. The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Major fatty acids were iso-C17:0 3-OH, summed feature 3 (C16:1 ω6c and/or C16:1 ω7c), and iso-C15:0. The genomic DNA G + C content was 37.4 mol%. Based on these polyphasic taxonomic data, strain MBLN094T is considered to represent a novel species of the genus Zunongwangia, for which the name Zunongwangia flava sp. nov. is proposed. The type strain is MBLN094T (= KCTC 62279T = JCM 32262T).
퍼지-신경회로망을 적용한 강인한 AC드라이브 시스템에 관한 연구
전희종,김재철,김병진,문학영,손진근,노남영,Jeon, Hee-Jong,Kim, Jae-Chul,Kim, Beung-Jin,Mun, Hark-Yong,Son, Jin-Geun,No, Nam-Young 한국조명전기설비학회 1997 조명.전기설비 Vol.11 No.1
본 논문에서는 퍼지제어기와 신경회로망 적응 관측기를 적용하여 강인성을 AC드라이브 시스템을 제안하였다. 퍼지제어기는 유도전동기의 속도 제어시 빠른 속도 응답 특성을 얻기 위하여 사용하였다. 신경회로망 적응관측기는 전동기 파라메터 변화에 대하여 강인한 제어 시스템이 되도옥 자속 관측기오 토오크 적응관측기로 구성하였다. 사용된 신경회로망은 자속과 토오크의 동특성을 학습시키기 위하여 역전파 알고리즘을 사용하였따. 컴퓨터 시뮬레이션의 결과를 통해 제안된 시스템이 전동기 파라메터 변동과 부화이론에 강인하고 속도응답 특성이 우수함을 입증하였다.
북극 스발바르 군도 중앙로벤 빙하 해안 지역의 토양 시료 내 메타지놈 기반 미생물 군집 분석
석윤지 ( Yoon Ji Seok ),송은지 ( Eun Ji Song ),차인태 ( In Tae Cha ),이현진 ( Hyunjin Lee ),노성운 ( Seong Woon Roh ),정지영 ( Ji Young Jung ),이유경 ( Yoo Kyung Lee ),남영도 ( Young Do Nam ),서명지 ( Myung Ji Seo ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.2
최근 빙하의 융해로 인해 빙하 해안 지역에 다양한 토양 미생물과 초목들이 드러나고 있다. 본 연구에서는 북극 스발바르 군도 중앙로벤 빙하 해안 지역으로부터 Ion Torrent Personal Genome Machine(PGM)을 활용한 메타지놈 분석을 통해 세균(bacteria), 고균(archaea), 및 진핵생물(eukaryotes)를 포함하는 다양한 미생물 군집을 분석하였다. 연구에 사용된 토양시료는 빙하 후퇴에 따른 토양의 노출 시기에 따라 2개 지역(ML4 및 ML7)으로부터 수집하였다. ML4 및 ML7 시료의 메타지놈 염기서열을 기반으로 총 2,798,108 및 1,691,859 reads가 각각 미생물 군집 분석에 활용되었다. Domain (계) 수준에서 미생물 군집의 상대 빈도를 분석한 결과 2개 시료 모두 세균(86-87%)이 높은 반면 고균과 진핵생물은 1% 미만으로 존재하는 것으로 나타났다. 또한 약 12%의 염기서열은 기존에 분류되지 않은(unclassified) 서열로 분석되었다. 세균의 경우 Proteobacteria(40.3% for ML4 and 43.3%for ML7)와 Actinobacteria(22.9% and 24.9%)가 우점하는 것으로 분석되었다. 고균의 경우에는 Euryarchaeota(84.4% and 81.1%)및 Crenarchaeota(10.6% and 13.1%), 그리고 진핵생물의 경우에는 Ascomycota(33.8% and 45.0%)가 우점하는 것으로 분석되었다. 본 연구를 통해 Ion Torrent PGM 플랫폼을 활용한 메타지놈 분석이 북극의 중앙로벤 빙하 해안 지역의 전체 미생물 군집 구조를 파악하는데 충분히 적용될 수 있을 것으로 사료된다. Recent succession of soil microorganisms and vegetation has occurred in the glacier foreland, because of glacier thawing. In this study, whole microbial communities, including bacteria, archaea, and eukaryotes, from the glacier foreland of Midtre Lovenbreen in Svalbard were analyzed by metagenome sequencing, using the Ion Torrent Personal Genome Machine (PGM) platform. Soil samples were collected from two research sites (ML4 and ML7), with different exposure times, from the ice. A total of 2,798,108 and 1,691,859 reads were utilized for microbial community analysis based on the metagenomic sequences of ML4 and ML7, respectively. The relative abundance of microbial communities at the domain level showed a high proportion of bacteria (about 86-87%), whereas archaeal and eukaryotic communities were poorly represented by less than 1%. The remaining 12% of the sequences were found to be unclassified. Predominant bacterial groups included Proteobacteria (40.3% from ML4 and 43.3% from ML7) and Actinobacteria (22.9% and 24.9%). Major groups of Archaea included Euryarchaeota (84.4% and 81.1%), followed by Crenarchaeota (10.6% and 13.1%). In the case of eukaryotes, both ML4 and ML7 samples showed Ascomycota (33.8% and 45.0%) as the major group. These findings suggest that metagenome analysis using the Ion Torrent PGM platform could be suitably applied to analyze whole microbial community structures, providing a basis for assessing the relative importance of predominant groups of bacterial, archaeal, and eukaryotic microbial communities in the Arctic glacier foreland of Midtre Lovenbreen, with high resolution.