자궁근염이 고양이 자궁의 감각수용기에 미치는 영향

나중렬,홍승길,이낙우,한희철,이민구 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.6

목적: 자궁내 염증을 유발하여 염증 후 일어나는 자궁을 지배하는 감각신경의 변화를 관찰하고, 염증시 유리되어 나오는 물질로 알려진 substance P가 자궁의 구심성 신경에 영향을 미치는 가를 알아보기 위해 실험하였다. 연구방법: 성숙한 암코양이 14마리를 사용하여 자궁동맥에 미세한 polyethylene tube를 삽입하여 자궁근내로 통증유발물질 및 기타 약품의 주입이 가능하게 하였으며 수술현미경하에서 하복신경을 중추 쪽으로 추적하여 확인한 후 기록용 백금전극에 올려놓았다. 기록전극 위에 놓인 하복신경의 활동전위는 증폭 및 여과장치를 거쳐 원하는 임펄스를 취한 후 이를 컴퓨터에 저장하고 분석하였다. 반응이 확인된 신경단위에 5ug의 bradykinin을 투여하고 반응을 기록한 후 자궁강내에 염증유발물질인 10%의 mustard oil을 papain[3mg/ml]처리한 후 주입하여 1시간 동안 방치하여 염증을 유도한 후 염증 후의 반응을 기록하였다. 다른 동물개체에서는 mustard oil 대신 염증시 유리되는 substance P를 5ug 자궁동맥내로 주입하여 주입전후의 bradykinin의 효과를 비교하여 염증시 유리되는 substance P의 효과를 관찰하였다. 결과: bradykinin에 대한 자궁 감각신경체의 반응성은 염증유발 2시간 및 4시간 후에 염증유발전보다 증가하였다. substance P 처리후에는 11개의 가는 유수신경[Aδ fiber] 중 8개가 반응성이 감소한 반면 10개 무수신경[C fiber] 모두에서 반응성이 증가하였다. 결론: 염증이 유발된 자궁에서 염증이 유발되기 전의 자궁에서보다 통증 유발물질인 bradykinin에 더욱 민감하게 반응하는 사실을 알 수 있었으며, 염증시 유리되는 substance P는 염증조직내의 구심성감각신경 중 특히 통증을 전달하는 C섬유군의 활성화에 중요한 역할을 하는 것을 알 수 있었다. 이와 같은 결과는 앞으로 내장감각기관에서 염증에 의한 주요증상의 하나인 통증의 기전을 이해하고 치료 및 약제 개발에 중요한 역할을 할 수 있으리라 생각된다. Objective : To examine whether the inflammation can alter the sensitivity of primary afferents innervating uterus and its adjacent tissues, and to elucidate the role of substance P which are known to be released to the inflamed tissues. Methods : Total fourteen female cats were used, four cats were used for inflammation tests. Uterine inflammation was induced by 10% of mustard oil after papain [3mg/ml] treatment. These chemicals were injected into the uterine cavity. Other 10 cats were used for substance P [5 g] tests which was injected into the uterine circulation. The responses of hypogastric nerve to bradykinin injected into the uterine circulation were recorded before and after either treatments [inflammation or substance P] using electrophysiological method. Results : The neuronal responses to bradykinin were increased at 2 hours and 4 hours after uterine inflammation. As for substance P treatment, the responses of 8 of 11 thin myelinated [A ] nerve fibers were decreased after substance P treatment, those of all 10 of unmyelinated [C] nerve fibers were increased. Conclusions : These results indicated that the afferents from the uterus responded to bradykinin more sensitively after inflammation and substance P which are released after inflammation may play a important role in uterine hyperalgesia.

Human Chorionic Gonadotropin과 Chorpromzine이 가토뇌조직(家兎腦組織)의 Catecholamime 함량에 미치는 영향

나중렬 대한산부인과학회 1976 대한산부인과학회 학술대회 Vol.38 No.-

Swyer Syndrome 1 례

나중렬,이용호,정윤돈,정수경,이응석,이정구 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.5

순수 생식선 이형성증은 매우 희귀한 예로 저자들은 본원에 내원한 18세의 여성에서 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. A 18-year-old female was referred on January, 1998 for primary amenorrhea. Physical examination revealed eunuchoid body proportions, no breast development and no pubic and axillary hair. No somatic abnormalities could be detected. Peripheral blood karyotype was 46, XY. Exploratory laparotomy revealed a hypoplastic uterus, bilateral tubes and bilateral streak gonads. It is well known that patients with gonadal dysgenesis in whom a Y chromosome component exists are at risk for developing gonadoblastoma or dysgerminoma. So, bilateral gonadectomy was performed and hormone replacement therapy was instituted. We report a case with review of the literature.

배란전 난포의 Steroid호르몬 형성에 대한 LH의

나중렬,홍성봉 대한산부인과학회 1988 Obstetrics & Gynecology Science Vol.31 No.11

임신중 알코올섭취가 태아발육 및 출생후 제반 생리적 신경반사에 미치는 영향

조태일,나중렬,홍승길 고려대학교 의과대학 1987 고려대 의대 잡지 Vol.24 No.1

Although liquid diets containing ethanol have been widely used in rodent models of the fetal alcohol syndrome, the nutritional adequacy of these diets and the effects of these treatment on the pregnent rat and subsequent pup development has not been sufficiently verified. Beginning on day 5 of gestation, maternal diet consumption, body weight and other physiologic parameters were monitored in rats receiving ethanol diet (Group A), paried-fed diet without ethanol (Group B) and ad libitum solid diet (Group C). The purpose of this investigation was to examine the effects of ethanol diet during pregnancy and postpartum on the developmental / reproductive parameters of pregnant rats and their offsprings. 1. Body weight of pups is suppressed by alcohol treatment - during the first 10 days postpartum, the slope of the increase in weight is significantly less than controls. - After removal of alcohol treatment, the slope of the growth curves are parallel but the ovarall weight of the alcohol treated rats is less. - The alcohol treated rats appear to be 4-6 days behind in growth when compared with controls. This appears to be true until day 8 of age. - At 80 days of age, the pre and postnatal alcohol treatment suppressed the body weight of male and female pups. 2. Duration of pregnancy is not affected by prenatal alcohol treatment, however, pellet diet rats gave birth one day earlier (p < 0.05) than alcohol treated rats. 3. Food intake was reduced (p < 0.01) in alcohol treated rats. The average was about 76 ml/day versus 119 ml/day in controls. 4. Weight gain of the mothers during pregnancy was reduced (p < 0.01) in the alcohol treated rats. The alcohol treated rats gained about 123 gm during days 5-20 of pregnancy whereas liquid and pellet controls gained 196 and 179 gm, respectively. 5. Litter size (number of pups) was not altered by alcohol treatment during pregnancy. 6. Birth weight was reduced by 18 and 10% when compared with liquid and pellet controls, however, not statistically significant. 7. Eye opening was delayed (p < 0.01) 2.4 and 1.4 days respectively, when compared with liquid and pellet diets. 8. Surface righting reflex was delayed (p < 0.01) by 3.7 days in the alcohol treated animals when compared with either controls. 9. Negative geotaxis was delayed (p < 0.01) 2.7 and 2.4 days respectively when compared with liquid and pellet diets. 10. Reflex suspension was delayed (p < 0.01) 3.3 and 3 days respectively when compared with liquid and pellet controls. It suggests that the maternal alcohol consumption plays an important role in the developmental and neurological events in the subsequent pups as described in this study.

폐경기 여성에서 골다공증의 위험인자들에 관한 연구

이찬,나중렬,신재철,이상희,이낙우 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.3

본 연구는 1995년 8월부터 1996년 6월까지 고려대학교 의과대학부속 안암병원 산부인과 외래에 내원한 폐경기 여성 88예를 대상으로, 골다공증의 조기진단과 치료 및 예방에 도움을 주고자 골다공증의 위험인자를 설문 조사와 골밀도 측정으로 분석하여 다음과 같은 결과를 얻었다. 1. 연령이 증가할수록, 폐경 기간이 길어질수록 골밀도가 감소하는 양상을 보였으며, 마른 체형을 가진 여성들에서 골밀도가 낮았다. 2. 갑상선 기능항진증이나 당뇨병, 골다공증의 가족력과 스테로이드나 이뇨제를 다량 복용한 기왕력이 있는 여성들에서 골밀도가 감소되어 있었다. 3. 흡연, 음주, 카페인, 운동 부족, 저칼슘 및 저 인섭취 등도 골밀도의 감소와 상관관계가 있는 것으로 나타났다. 4. 폐경 연령, 초경 연령, 임신력, 폐경 원인 등은 골밀도에 영향을 미치지 않는 것으로 나타났다. 5. 연령 증가, 폐경 기간, 체중 등은 정상군에 비해서 골다공증군에서 유의한 차이를 보여 골다공증의 위험인자로서 분석되었다. 노령 인구 및 폐경 여성의 인구가 급격히 증가하고 있는 우리 나라에서도 골다공증의 예방에 힘 쏟아야 할 때라고 생각되며, 골다공증의 위험인자를 가진 폐경 여성들에게는 호르몬 대체요법을 권하여야 할 것으로 사료된다. The osteoporosis, one of the most common disorder of the human skeleton, is characterized by a decreased bone mineral density, inceased porosity of the bones, and a decreased resistance to fracture. The incidence of osteoporotic fractures in old postmenopausal women has increased enormously during the past decades, making a significant health and economic problem. Efforts to retard or prevent the development of osteoporosis have been performed in the developed countries. The main risk factors for osteoporosis are associated with the female sex, age and loss of ovarian function. Several other risk factors have also been described, including low physical activity, low body weight and low calcium intake. Also smoking, excessive use of alcohol and coffee as well as several diseases and drugs seem to affect bone mineral metabolism. These studies were performed to evaluate the risk factors of osteoporosis in menopausal women and also protect from the osteoporosis. Dual photon X-ray absorptiometry[DPXA] was employed to measure bone mineral density in 88 menopausal women and the osteoporosis was defined when the bone mineral density of the patients was below -2 standard deviation of Z score. The results were as follows ; 1] Bone mineral density showed a linear reduction with age, duration of menopause, and decreased in thin women. 2] Bone mineral density decreased in women with hyperthyroidism and diabetes mellitus, family history of osteoporosis, and long term use of corticosteroid and diuretics. 3] Smoking, alcohol, caffeine, no regular exercise, low calcium and phsophorus diet, and lactose intolerance were related to lower bone mineral density. 4] There were no relationship between age at menopause, parity, cause of menopause and bone mineral density. 5] Age, duration of menopause and weight were defined as the risk factors for osteoporosis in menopausal women.

Dehydroepiandrosterone sulfate (Mylis)R의 자궁경부 숙화작용에 대한 영향

허준용,양현모,나중렬,구병삼,홍성봉 대한산부인과학회 1984 대한산부인과학회 학술대회 Vol.53 No.-

일반연제 발표 : 체외수정과 배이식에 의한 임신성공 및 분만(分娩)에 관한 연구

구병삼,이규완,나중렬,홍성봉 대한산부인과학회 1986 대한산부인과학회 학술대회 Vol.58 No.-

외음부 Condylomata accuminata Lesion에 LASER광(光)의 치료(1)

구병삼,이규완,나중렬,홍성봉 대한산부인과학회 1986 대한산부인과학회 학술대회 Vol.58 No.-

최신임상강좌 : 상피성난소종양에서 clusterin, bax, p53, Ki-67의 발현 및 세포사멸지수에 관한 연구

이지영 ( Ji Young Lee ),나중렬 ( Jung Yeol Na ),김인선 ( In Sun Kim ),박문향 ( Moon Hyang Park ),강재성 ( Jae Seong Kang ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.9

목적: 상피성난소종양에서 clusterin, bax, Ki-67, p53의 발현과 세포사멸지수에 관해 알아보고, 각 지표의 발현이 상호 간에 어떤 연관성을 갖는지, 각 지표의 발현이 전체 생존율과 연관이 있는지 알아보고자 하였다. 연구 방법: 낭선종 22예, 경계악성 상피난소종양 44예, 상피성난소암종 96예의 조직학적, 임상병리학적 양상을 후향적으로 분석하였다. 파라핀 포매조직을 이용하여 면역조직화학염색을 시행하여 clusterin, bax, Ki-67, p53의 발현 양상과 세포사멸지수를 분석하였다. 결과: Clusterin, p53, Ki-67의 과발현은 상피성난소암종에서 유의하게 많았고, p53과 Ki-67의 과발현은 높은 병기, 나쁜 분화도, 양측성인 상피성난소암종에서 유의하게 많았다. 세포사멸지수는 bax가 과발현된 군에서 높았으나 clusterin이나 p53의 발현과는 유의한 연관성이 없었다. Clusterin, p53 및 Ki-67의 과발현은 상피성난소암종 환자의 전체 생존율과 유의한 연관성이 없었다. 결론: 난소암종 환자에서 p53과 Ki-67은 분화도가 나쁜 난소암종에서 과발현하여 환자의 예후를 예측하는 지표자로 활용될 수 있다. Clusterin의 과발현은 경계악성 피난소종양보다 난소암종에서 많아 향후 clusterin 과발현을 보이는 상피성난소암종 환자에서 표적치료에 대한 연구를 시도할 가치가 있다. Objective: The purpose of this study was (1) to evaluate the expressions of clusterin, bax, Ki-67, p53, and apoptotic index in epithelial ovarian tumors, borderline and malignant ovarian tumors, (2) to find out the correlation between their expressions and clinicopathological parameters, and (3) to evaluate the effect on the patient`s survival according to their expressions. Methods: The histological and clinical findings of 22 cases of ovarian cystadenomas, 44 cases of borderline tumors and 96 cases of carcinomas were evaluated. Expressions of clusterin, bax, Ki-67, p53, and apoptotic index were studied on paraffin-embedded tissue sections by immunohistochemical methods. Results: The expressions of clusterin, p53, and Ki-67 were higher in ovarian carcinomas than borderline tumors. The overexpression of p53, and Ki-67 were frequent in high stage, poorly differentiated and bilateral ovarian carcinomas. The overexpressions of clusterin, bax, p53, and Ki-67 showed a statistically significant correlation with histologic type. Apoptotic index was higher in bax overexpression group, but there was no correlation with overexpression of clusterin or p53. Ki-67 was higher in p53 overexpression group, but there was no correlation with overexpression of clusterin or p53. There was no statistically significant correlation with each other between the overexpressions of clusterin, bax, p53, and Ki-67. The overexpressions of clusterin, Ki-67, p53 was associated with overall patient`s survival in borderline significance. Conclusion: The overexpression of p53, and Ki-67 were frequent in poorly differentiated ovarian carcinomas. So the overexpression of p53, and Ki-67 can be used as prognostic factor. The overexpression of clusterin was more in epithelial ovarian carcinomas than in borderline tumors but showed no significant correlation with the overall patient`s survival. Further studies are required to clarify the possibility of using clusterin for target therapy in epithelial ovarian carcinomas.