Brucella abortus 국내 분리주의 Heat Shock Protein 암호 groE 유전자의 염기서열 분석과 발현

김태용,김지영,장경수,김명철,박창식,한홍율,전무형,Kim, Tae-Yong,Kim, Ji-Young,Chang, Kyung-Soo,Kim, Myung-Cheol,Park, Chang-Sik,Han, Hong-Ryul,Jun, Moo-Hyung 대한수의학회 2005 大韓獸醫學會誌 Vol.45 No.1

GroE that is a heat shock protein composed of GroEL and GroES is known as an immunodominant target of both the humoral and cellular immune responses in bovine brucellosis. This study was carried out to characterize groE gene encoding heat shock proteins of B. abortus isolated in Korea and to evaluate the immunogenicity of the GroE protein expressed in E. coli system. In PCR the specific signals with the size of 2,077 bp were detected in five strains isolated from the mammary lymphnodes of the dairy cattle that were serologically positive and the reference strains. In comparison of the sequences of nucleotides and amino acids among the strains, GroES showed 100% identity in both sequences. GroEL was evaluated 99.0~99.9% in nucleotides and 98.0~100% homology in amino acids. The groE gene including groES and groEL was inserted into pET29a vector and constructed pET29a-GroE recombinant plasmids. The inserted groE was confirmed by digestion with Nco1 and EcoR1 endonucleases and nucleotide sequencing. E. coli BL (DE3) was transformed with pET29a-GroE, named as E. coli BL (DE3)/pET29a-GroE. In SDS-PAGE, it was evident that the recombinant plasmid effectively expressed the polypeptides for GroES (10 kDa) and GroEL (60 kDa) in 0.5, 1 and 2 hours after IPTG induction. The immuno-reactivity of the expressed proteins were proved in mouse inoculation and Western blot analysis.

Degradation Behavior of 850 nm AlGaAs/GaAs Oxide VCSELs Suffered from Electrostatic Discharge

김태용,Taeki Kim,Sangin Kim,Sang-Bae Kim 한국전자통신연구원 2008 ETRI Journal Vol.30 No.6

The effect of forward and reverse electrostatic discharge (ESD) on the electro-optical characteristics of oxide vertical-cavity surface-emitting lasers is investigated using a human body model for the purpose of understanding degradation behavior. Forward ESD-induced degradation is complicated, showing three degradation phases depending on ESD voltage, while reverse ESD-induced degradation is relatively simple, exhibiting two phases of degradation divided by a sudden distinctive change in electro-optical characteristics. We demonstrate that the increase in the threshold current is mainly due to the increase in leakage current, nonradiative recombination current, and optical loss. The decrease in the slope efficiency is mainly due to the increase in optical loss.

역문제를 이용한 2차원 산란장에서의 소스 추정

김태용,이훈재,Kim, Tae Yong,Lee, Hoon-Jae 한국정보통신학회 2014 한국정보통신학회논문지 Vol.18 No.6

역문제는 특히 시스템 모델링 및 모니터링과 같은 과학 및 공학분야에서 매우 흥미로운 주제이다. 역문제를 활용하면 광물자원의 탐사, 지하에 매설된 케이블 및 파이트라인 등의 동정, 의료 분야에서의 비파괴 검사 등에 응용가능하다. 본 연구에서는 먼저 2차원 전자계 산란문제를 다루고 이에 대한 FDTD법 정식화를 다룬다. 이 경우 근방계에서 관측된 데이터를 토대로 미지의 소스를 추정하기 위한 방법으로서 비선형 CGM법을 이용하였다. 본 연구에서 제안된 방법에 따른 실험결과로 볼 때 임베디드 시큐리티 모듈과 같은 원 신호 추정문제에 적용 가능할 것으로 보인다. Inverse problem is very interest in the sciences and engineering, in particular for modeling and monitoring applications. By applying inverse problem, it can be widely used to exploration of mineral resources, identification of underground cables and buried pipelines, and diagnostic imaging in medical area. In this paper, we firstly consider 2-dimensional EM scattering problem and present the FDTD method to estimate unknown source. In this case, non-linear CGM technique is used to investigate unknown sources corresponding to measured data obtained from forward problem in near field. The proposed technique for solving the inverse source problem presents a reasonable agreement and can be applied to investigate an internal source signal of embedded security module.

멀티스레드 기반 PO법 시뮬레이션

김태용,이훈재,Kim, Tae-Yong,Lee, Hoon-Jae 한국정보통신학회 2011 한국정보통신학회논문지 Vol.15 No.11

현재 범용성이 높은 전자계 시뮬레이터가 널리 보급되어 왔으며, 안테나 설계, EMC 설계, 측정, 초고주파 소자 설계 등에 활용되고 있다. 본 연구에서는 X 밴드 영역에서 다양한 전자계 문제 해석을 목적으로 멀티 코어 기반 PC 자원을 보다 효율적으로 활용하고, 나아가 TCP/IP 기반 네트워크 토폴로지 구성을 통한 효율성 높은 전자계 시뮬레이터 구현을 위한 프레임워크 구축과 구현 방안을 제안하고 그 유효성을 검증하였다. Current general-purpose electromagnetic field simulators have been widespread applied, and is being used to electromagnetic problems such as antenna design, EMC design, measurement, and microwave device design, etc. This paper is to solve various electromagnetic problems in X-band region for utilizing multi-core-based PC available network resources more efficiently. The electromagnetic field simulator based on TCP / IP-based network topology, configuration, and its framework design is proposed and its availability is examined.

열증으로 변증된 배뇨곤란 환자의 자신환 치험 3례

김태용,오민규,신현수,한종현,박준범,Kim, Tae-Yong,Oh, Min-Kyu,Shin, Hyeon-Su,Han, Jong-Hyun,Park, Jun-Beom 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.4

Urinary disturbance is deficit in volitional control of voiding. Stroke can cause temporary or permanent urinary disturbance. The purpose of this study was to confirm the effect of Jasin-hwan on urinary disturbance as heat syndrome. This study was carried out on 3 patients with urinary disturbance caused by stroke. We measured components of IPSS (international prostate symptom score) at pre-treatment and post-treatment. After prescribing medication to the patients, symptoms improved rapidly within three weeks. These results support that Jasin-hwan is effective in reduction of urinary disturbance as heat syndrome.

비침투형 공격에 강한 다중 유전체 코팅 설계

김태용,이훈재,Kim, Tae-Yong,LEE, HoonJae 한국정보통신학회 2015 한국정보통신학회논문지 Vol.19 No.6

In general, IC chip circuit which is operating a cryptographic computation tends to radiate stronger electromagnetic signal to the outside. By using a power detecter such as a loop antenna near cryptographic device, the encryption key can be identified by probing a electromagnetic signal. To implement a method against non-invasive type attack, multi dielectric slab structure on IC chip to suppress radiated electromagnetic signal was introduced. Multiple dielectric slab was implemented by suitably configured to have the Bragg reflection characteristics, and then the reflection response was computed and verified its effectiveness. As a result, the thickness of the dielectric coating was 2mm and the reflection response characteristics for the vertical incidence was achieved to be 91% level. 일반적으로 암호연산이 수행되는 IC 칩 회로는 강한 전자기 신호를 외부로 방사시키는 경향이 있다. 암호 칩 근방에서 루프 안테나와 같은 전력 수집 계측 장비를 활용하면 계측된 전자기 신호에 의해 암호 키의 동정이 가능하다. 이와 같은 비침투형 공격에 대응하기 위한 한 방법으로서 IC 칩 외부로 방사되는 전자기 신호를 억압하기 위해 칩 상부에 다중 유전체 슬래브 구조를 가지도록 구성하는 방법을 도입하였다. 다중 유전체 슬래브는 Bragg 반사특성을 가지도록 적절하게 구성하여 구현하였고 반사응답 특성을 구하여 그 유효성을 검증하였다. 실험결과로서 유전체 코팅의 두께는 2mm로서 수직 입사파에 대한 반사응답 특성은 91% 수준을 달성하였다.

FDTD법을 이용한 무선 환경에서의 회절 특성

김태용,이훈재,Kim, Tae Yong,Lee, Hoon-Jae 한국정보통신학회 2013 한국정보통신학회논문지 Vol.17 No.6

무선 LAN과 같은 전파환경 내에서 무선 AP 등의 적절한 배치를 통하여 효율적인 서비스 반경을 확보할 수 있다. 실험을 위해서 FDTD법을 이용하여 정식화하고, 무선 AP 대신에 계산 공간의 임의 위치에서 가우시안 펄스를 입력하였다. 장애물의 위치에 따른 구조적 영향을 고려하고 이에 따른 수신 이득을 계산하였다. 장애물은 완전 도체 또는 콘크리트 벽면으로 모델링하고, 장애물을 사이에 둔 송신점과 수신점 사이에서의 회절효과 등의 영향을 평가하였다. In wireless environment such as wireless LAN, proper location of wireless LAN AP allows us to take an effective coverage to provide good wireless communication service. To simulate wireless environment, FDTD formulation is introduced and Gaussian pulse injection in grid space is considered to be replaced wireless LAN AP. So structural effect corresponding to location of arbitrary obstacle must be considered, and its received gain is estimated. An obstacle in scattering field should be modeled to be conductor and concrete wall and its diffraction effect for some cross wall is evaluated between transmitter and receiver point.

다중금속복합층 핵연료 피복관의 필거링 공정에 관한 유한 요소 해석 연구

김태용,이정현,김지현,Kim, Taeyong,Lee, Jeonghyeon,Kim, Ji Hyun 한국압력기기공학회 2017 한국압력기기공학회 논문집 Vol.13 No.2

In severe accident conditions of light water reactors, the loss of coolant may cause problems in integrity of zirconium fuel cladding. Under the condition of the loss of coolant, the zirconium fuel cladding can be exposed to high temperature steam and reacted with them by producing of hydrogen, which is caused by the failure in oxidation resistance of zirconium cladding materials during the loss of coolant accident scenarios. In order to avoid these problems, we develop a multi-metallic layered composite (MMLC) fuel cladding which compromises between the neutronic advantages of zirconium-based alloys and the accident-tolerance of non-zirconium-based metallic materials. Cold pilgering process is a common tube manufacturing process, which is complex material forming operation in highly non-steady state, where the materials undergo a long series of deformation resulting in both diameter and thickness reduction. During the cold pilgering process, MMLC claddings need to reduce the outside diameter and wall thickness. However, multi-layers of the tube are expected to occur different deformation processes because each layer has different mechanical properties. To improve the utilization of the pilgering process, 3-dimensional computational analyses have been made using a finite element modeling technique. We also analyze the dimensional change, strain and stress distribution at MMLC tube by considering the behavior of rolls such as stroke rate and feed rate.

환경산업의 새로운 변화와 성장 동력

김태용,Kim, Tae-Yong 환경보전협회 2006 환경정보 Vol.28 No.363

간혈부족(肝血不足)으로 변증한 진전(振顫)환자 치험(治驗) 1례(例)

김태용,박준범,오민규,신현수,Kim, Tae-Yong,Park, Jun-Beom,Oh, Min-Kyu,Shin, Hyeon-Su 대한한방내과학회 2006 大韓韓方內科學會誌 Vol.27 No.3

Tremor is a rhythmic and involuntary muscular contraction characterized by oscillations of a part of the body. The most common of all involuntary movements, tremor can affect various body parts such as hands, head, facial structures, vocal cords, trunk, and legs; most tremors, however, occur in the hands. Clinically, tremor is classified into postural tremor, resting tremor, action tremor, and other kinds of tremor, and treated according to the causes. The author reports the improvement of the patient, hospitalized at Dong Seo Medical Center, whose tremor was not classified specifically in western medicine but was diagnosed as Ganhyeolbujok (肝血不足) by Korean medicine.