생리 : 주간의 슬관절 고정이 근력 및 혈청 효소 활성도에 미치는 영향

김양수,김성수,위승두,방상식,문원재,김창근 ( Yang Soo Kim,Sung Soo Kim,Seung Doo Wee,Sang Sik Bang,Won Jeh Mun,Chang Keun Kim ) 한국체육학회 1992 국제스포츠 학술대회 Vol.1992 No.-

체세포 복제란 이식우의 분만 전.후 TGF-${\beta}_1$ 단백질 농도

황성수,장유민,고응규,양병철,임기순,김명직,민관식,윤종택,김창근,성환후,Hwang, Seong-Soo,Chang, Yoo-Min,Ko, Yeoung-Gyu,Yang, Byong-Chul,Im, Gi-Sun,Kim, Myong-Jik,Min, Kwan-Sik,Yoon, Jong-Taek,Kim, Chang-Keun,Seong, Hwan-Hoo 한국동물번식학회 2008 Reproductive & developmental biology Vol.32 No.1

본 연구는 체세포 복제란 이식우의 분만에 있어서 혈중 스테로이드호르몬, TGF-${\beta}_1$ 농도와 분만지연의 상관 관계를 살펴보고자 실시하였다. 대조군으로는 인공수정(AI)을 통하여 임신한 암소(cow)들을 사용하였다(AI-R). 모든 AI-R들은 자연분만(n=5, 임신 $284{\pm}0.71$일)을 하였다. 분만징후를 보이지 않는 체세포 복제란 이식우(n=5, SCNT-R)들은 분만 예정일보다 10일 정도 지난 임신 292일째에 제왕절개(Caesarean section, C-sec)를 실시하여 분만하였다. 혈액 및 태반 샘플을 분만 전.후에 채취하여 형태 및 중량 등을 측정하였다. 혈장호르몬인 Progesterone(P4)와 Estradiol-$17{\beta}$(E2) 농도는 방사선동위원소 면역 분석 시험(RIA) 방법을 이용하여 측정하였다. 혈장 및 태반분엽의 TGF-${\beta}_1$ 농도는 ELISA 방법으로 측정하였다. SCNT-R에서 회수한 태반의 무게는 AI-R의 것과 비교하여 유의적으로 무거웠다(p<0.05). 분만 직전 SCNT-R들의 혈장 내 P4 농도는 AI-R들의 그것과 비교하여 유의하게 높았다(p<0.01). 하지만 SCNT-R들의 혈장 내 E2 농도는 AI-R과 비교하여 상대적으로 낮게 나타났다(p<0.01). 한편, 분만 전.후 SCNT-R들에서 혈장 또는 태반분엽의 TGF-${\beta}_1$ 단백질 발현 수준은 AI-R들과 비교하여 각각 유의적으로 높은 수준을 유지하였다(p<0.01). 이상의 결과를 종합하여 보면, 분만 시 P4 및 E2의 이상 발현과 높은 수준의 혈장 및 태반 내 TGF-${\beta}_1$ 단백질은 체세포 복제태아의 분만지연을 야기하는 중요한 요인들 중의 하나일 것이라 사료된다. This study was performed to investigate the correlations between steroids and TGF-${\beta}_1$ levels and delayed parturition in SCNT clone calving. The recipients pregnant by AI were used as control (AI-R). All AI-R were labored by natural delivery (n=5, day $284{\pm}0.71$ of pregnancy). The recipients pregnant by SCNT embryo (SCNT-R) showing no signs of delivery about 10 days after expected date were operated by Caesarean section (n=5, day 292). The blood and placentome samples were obtained and weighed at parturition. The concentrations of plasma progesterone (P4) and Estradiol-$17{\beta}$ (E2) were measured by radioimmunoassay (RIA). The levels of plasma and placental TGF-${\beta}_1$ levels were examined by ELISA. The placentomes from SCNT-R were overweight (p<0.05) compared to those of AI-R. The plasma P4 (p<0.01) level in SCNT-R at parturition was significantly higher compared to that of AI-R. In contrast, the plasma E2 level in the SCNT-R was significantly lower compared to that of AI-R (p<0.05). The plasma and placental TGF-${\beta}_1$ protein levels in the SCNT-R were significantly higher than those of AI-R at parturition, respectively (p<0.01). Based on these results, aberrant expressions of steroid hormones and high levels of plasma and placental TGF-${\beta}_1$ protein at parturition may be one of the key indicators on delayed parturition of SCNT clone calving.

인종에 따른 태권도 선수들의 유·무산소 능력의 차이

이길우,정용수,권영우,양대승,문원재,이승국,김창근 韓國體育大學校附屬 體育科學硏究所 1996 韓國體育大學校附屬 體育科學硏究所論文集 Vol.15 No.1

The racial differences in exercise performance was determined. Eleven subjects who well trained athletes in martial arts(Tae Kwon Do) participated in this study from Nigeria(n=7) and Egypt(n=4). Wingate Anaerobic Power Test(WAPT) and Åstrand Incremental Test were performed to measure the differences in aerobic and anaerobic power capacity. The results of this study showed that there was no significant difference in lactate concentration immediately after WAPT and during recovery. The recovery rate of blood lactate in Nigerian athletes was about 8% higher than that of Egyptian athletes. There were no significant differences in Mean Power Output level than that of Egyptian athletes. The recovery rate of heart rate at 10min after Åstrand incremental test was about 5% higher in Egyptian athletes than that of Nigerian athletes. The time to exhaustion with Åstrand incremental test was slightly longer in Nigerian athletes. In conclusion, aerobic and anaerobic capacity were somewhat higher in Nigerian Tae Kwon Do players and a mark interindividual differences in aerobic and anaerobic power existed in this study. Therefore, further studies must be performed before definitive conclusions can be made to generalize racial differences in performance in specific sports events.

ATPase 염색법에 의한 근섬유 형태구분

김양수,김창근 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

장시간 싸이클 주행중 혈장 글루코스,젖산,소디움 및 포타시움 농도의 변화

김창근,김근우,김양수,김학렬,최용어,홍경란 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

장시간 최대하 싸이클 주행중 근피로를 파악하기 위한 지표로서 소디움, 포타시움 농도의 변화양상을 살펴보고자 대학 대표급 싸이클 선수 14명을 대상으로 롤러위에서 시속 40 - 41km의 싸이클 주행속도를 유지할 수 있도록 조절하여 120분동안 수행하도록 하였다. 혈장 글루코스, 젖산, 소이움 및 포타시움 농도의 측정을 위한 혈액쌤플은 새벽 6시 기상직후와 실험시작전(0), 운동 30분, 60, 90, 120분에 주전정맥에서 약 5ml의 혈액을 채혈하였다. 혈장 글루코스와 젖산농도는 spectrophotometer를 이용하여 효소법으로 측정하였으며 소디움, 포타시움 농도는 Flame photometer를 이용하여 standard method로 측정하였다. 혈장 글루코스와 포타시움 농도는 운동 전체를 통하여 큰 변화가 없는 것으로 나타났으며(P>.05) 젖산 및 소디움 농도는 운동의 시간경과에 따라 증가되는 경향(P<.05)을 나타내었으나 운동부하에 따른 변화폭은 적은 것으로 나타났다. 그러나 혈장 소디움 농도는 혈장의 젖산농도와 함께 유사한 변화양상을 나타냄으로서 다소의 관련성을 엿볼수 있어 이의 규명을 위한 계속적인 연구가 요구된다. The purpose of this study was to determine a significance of sodium (Na??) and potassium(K??) concentration with indicators for estimate a muscular fatigue during prolonged submaximal cycle race. Forteen endurance-trained cyclists were used as subjects in the study. The exercise protocol consisted cycle race for 120min on the rollers cycle. The blood sample for measurement of plasma glucose, lactate, sodium and potassium concentration were drawn to A.M.6:00, pre-cycling(0), 30, 60, 90, and 120 min of cycling by collected about 5ml in the antecubital vein. Plasma glucose and lactate concentration measured by enzymatic technique using spectrophotometer, also sodium and potassium concentration measured by standard method using flame photometer. Plasma glucose and potassium concentration was not significance difference(P>.05) during prolonged cycle exercise, but plasma lactate and sodium concentration was shown increase during time course of prolonged cycle race(P<.05). However, plasma lactate and sodium concentration shown increase of small range during cycle race. Sodium concentration was requested continuous research by shown simillar patterns with plasma lactate concentration.

장기간의 유산소운동이 폐확산 능력에 미치는 영향

金昌根,金陽洙 韓國體育大學校附屬 體育科學硏究所 1990 韓國體育大學校附屬 體育科學硏究所論文集 Vol.9 No.1

The purpose of this study was to investigate long term aerobic training effect on diffusing capacity of lungs in female tennis players. 14 female tennis players belonged to professional tennis teams and 9 controls were participated in this experiment. Mean diffusing capacity of lungs for carbon monoxide(DLco) of tennis player were 30.2ml/min/mmHg which was significantly higher than that of controls. Alveolar ventilation of tennis players was 5.95 liter and that of controls was 4.52 liter, so experimental group was significantly different(P < 0.001) from controls. The ratio of DLco/Va was revealed that measured value was higher than preadicted value in tennis players, bur the measured value of controls was lower than predicted value. the ratio of exprimental group was showed 108% and that of controls was showed 94.5%. Krogh s constant of tennis players was 4.35 min, which was 87.7% of their predicted value and that of control group was 3.7 min, which was 74% in their predicted value. Both tennis players and controls of measured values were revealed. significantly lower than their predicted values. Although diffusion was made by partial pressures of gas between alveolar air and blood supply, diffusing capacity of lungs were influenced through long term aerobic exercise.

Myosin Heavy Chain Coexistance in human Skeletal muscle

Kim,Hag Lyeol,Kim,Yang Soo,Kim,Chang Keun 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

2주간의 슬관절 고정이 근력 및 혈청 효소 활성도에 미치는 영향

김양수,김창근,방상식,위승두,문원재,노재금,김성수 고려대학교 스포츠과학연구소 1992 스포츠科學論叢 Vol.3 No.1

Removal of weight bearing of skeletal muscle by limb immobilization produced atrophy of slow twitch muscles fixed at resting length. The initial decreases of muscle artrophy started with an early, large decrease in protein synthesis rate. Muscle strength and enzyme activity changes induced in skeletal muscle as a result of joint immobilization, glycolytic enzyme activity in fast muscle and oxidative enzyme activity in slow muscle decreased remarkably following hypodynamia, immobilization. However, The aim of present paper was to investigated the effects of one leg immobilization during the two weeks to the muscle strength and serum enzyme activities. The results was summarized as follows; 1. Immobilized leg was significantly decreased muscle strength(ext;27.9% flx:8.5%), power(ext:15.5% flx:6.5%) and muscle endurance(ext:8.8% f1x;10.3%) which was 50 repetition work capacity at 180 degree/sec, during the two week immobilization, opposed leg was slightly increased strength, power and muscle endurance to extensor and flexor muscle. 2. CPK activity in serum was increased significantly in the control group, but that was decreased in the experimental group. GPT and GOT was showed to be decreased slightly in the control group but, to be increased the experimental group. 3. LDH was decreased after two weeks immobilization control and experimental groups. After two weeks immobilization, LD1 isozyme in experimental group was down significantly(P$lt;0.01) lower than that of control. LD4 and LD5 isozymes revealed to be increased significantly after two weeks in the experimental group. Therefore sudden stop by immobilization effected to decrease muscle strength, power and endurance including enzyme activities to influence subtly energy supplement. So we should study not to damage injured athletes and the off season.

Weight training 이 근력 및 근섬유 형태 변화에 미치는 영향

방상식,이근일,김학렬,김양수,김창근,원신희,안효작 韓國體育大學校附屬 體育科學硏究所 1991 韓國體育大學校附屬 體育科學硏究所論文集 Vol.10 No.1

Eight healthy males performed moderate training program 3days per week for 10weeks. Strength was measured before and after weight training program using a Cybex Ⅱ isokinetic dynamometer set an angular velocity at 60???S?? muscle strength, 180???S?? muscle power, 180????S??50 repetition muscle endurance, respectively. Isotonic one repetition maximum (1RM) was measured before and after training program. Muscle biopsies were taken before and after training from vastus lateralis muscle. Obtained muscle sample serially was cutted with 10??m thick and stained for myofibrillar ATPase with preincubation at pH 10.3, 4.6, and 4.37 for identifing type Ⅰ, Ⅱa, Ⅱb and Ⅱc fibers, resectively. Peak power output was 424 watt and increased by 10.5% after the weight training. Weight training resulted in significant increase one repetition maximum for each types of exercise. Weight training resulted in significant hypertrophy of all types : type Ⅰ (24%), typeⅡa(30%), typeⅡa(9.4%), typeⅡc(35%). In addition, the training resulted in a decrease capillaries of type Ⅰ, Ⅱa, Ⅱb fibers with a concomitant increase in type Ⅱc fibers, suggesting that moderate weight training may lead to muscle hypertrophy and possible fiber type conversions.