갯기름나물의 체세포배 분화와 기관 분화를 통한 재분화

김옥태,김광수,안준철,황백,Kim, Ok-Tae,Kim, Kwang-Soo,An, Jun-Chul,Hwang, Baik 한국식물생명공학회 2001 식물생명공학회지 Vol.28 No.1

식용 및 약용으로 사용되는 갯기름나물의 기내배양을 통한 재분화율을 조사하였다. 캘러스는 잎과 엽병의 절편체에서 발생하였고 0∼5 mg/L의 NAA와 0∼10 mg/L BA가 첨가된 MS 기본배지에 치상하여 암배양 결과 자발적인 배 분화와 기관 분화를 보여주었으며 2.5 mg/L NAA와 10 mg/L BA의 조건에서 재분화율이 가장 높았다. 체세포 배의 발생양상은 구형, 심장형, 어뢰형을 거쳐 자엽 단계로 발달하여 전형적인 접합자 배의 모습으로 발달하였다. 배 발생한 일차 체세포배를 0.2 mg/L의 NAA가 첨가된 배지에서 배양하였을 때, 이차 체세포배가 발생하였으며 주로 일차 체세포배의 배축과 하배축에서 많이 발생하였고 그 다음으로 자엽이나 뿌리 부분에서 발생하였다. Peucedanum japonicum $T_{HUNB}$ used as a edible and medicinal plants was investigated for in uitro regeneration. Callus formation occurred on leaf and stem explant cultures and showed spontaneous embryogenic and organogenic capability on MS basal medium supplemented with 0.1~5 mg/L NAA and 0~10 mg/L BA in dark. The regeneration was highest on the condition supplemented with 2.5 mg/L NAA and 10 mg/L BA. Development of the somatic embryo progressed through the globular, heart-shaped, torpedo-shaped and cotyledonary stage, typical of zygotic embryos. When the first somatic embryos was cultured on the medium supplemented with 0.2 mg/L NAA, secondary somatic embryo were induced with higher frequency on the hypocotyl then on the cotyledon and root.t.

병풀 (Centella asiatica L. Urban) 식물체 배양을 이용한 Triterpene Glycoside 생산

김옥태,김민영,박윤정,홍민희,안준철,오만호,황백,Kim, Ok-Tae,Kim, Min-Young,Park, Yoon-Jung,Hong, Min-Hee,An, Jun-Chul,Oh, Man-Ho,Hwang, Baik 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4

기내에서 병풀의 식물체 배양을 확립하였고 기본배지 설정 및 대량원소들, sucrose 농도가 triterpene glycoside (Made-cassoside와 Asiaticoside: M&A) 생산에 대한 영향을 조사하였다. 병풀 식물체가 각각의 배지에서 배양되었을 때, MS와 RCM배지는 각각 식물체 생장과 M&A 생산에 대해 가장 좋은 결과를 나타냈다. 하지만, M&A에 대해 가장 높은 생산성은 B5 배지로 나타내었다. B5 배지의 대량영양소인 KNO$_3$는 25 mM, NaH$_2$PO$_4$는 1 mM, CaC1$_2$는 1 mM 그리고 MgSO$_4$는 1~10 mM에서 M&A 생산이 최적을 이루거나 증가시켰다. Sucrose를 농도별로 처리했을 때, 6% sucrose 농도에서 가장 높은 M&A 생산을 얻을 수 있었다. Whole plant cultures of Centella asiatica (L.) Urban in vitro were established and the effects of basal media, some macro elements and sucrose concentration on productivity of triterpene glycosides (madecassoside and asiaticoside: M$\varepsilon$A) were investigated. Among the media (MS, B5, RCM) tested, MS and 0.5 RCM medium were the best for plant growth and M$\varepsilon$A production, respectively. However, taking into account the M$\varepsilon$A productivity, B5 medium was superior (M$\varepsilon$A: 14.28 mg/g dry wt.). Major macronutrients of B5 medium adjusted with the concentration of 25 mM KNO$_3$,1 mM NaH$_2$PO$_4$, 1 mM CaCl$_2$ and 1~10 mM MgSO$_4$, caused elevated or optimized levels of M$\varepsilon$A. On sucrose concentration, the highest yields of M$\varepsilon$A were obtained from 6% sucrose.

Cloning and Molecular Analysis of cDNA Encoding Cycloartenol Synthase from Centella asiatica (L.) Urban

김옥태,황성진,안준철,황백,Min-Young Kim 한국생물공학회 2005 Biotechnology and Bioprocess Engineering Vol.10 No.1

cDNA for oxidosqualene cyclase was cloned by a homology-based PCR method and sequenced from Centella asiatica. In a sequences analysis, the putative polypeptide of C. asiatica cycloartenol synthase (CaCYS) deduced from the 2,274 bp nucleotide sequence, consisted of 758 amino acids and had a molecular mass of 86.3 kD. The predicted amino acid sequence exhibited high homology to that of PNX (cycloartenol synthase) from Panax ginseng (89%). Southern blot analysis suggests that CaCYS may be present in one copy of the C. asiatica genome. If methyl jasmonate (MJ) is applied exogenously to plants, not only triterpene saponins are accumulated in tissues, but also it produces effects such as growth inhibition and the promotion of ethylene production. In order to investigate the effect of MJ and thidiazuron (TDZ), a cytokinin that plays a role as an antisenescence agent in several plants, on the level of CaCYS mRNA, we performed northern blot analysis. When MJ is alone treated by adding to culture medium, CaCYS transcripts were inhibited. However, sustained levels of the expression of CaCYS, by adding TDZ to the medium despite MJ treatments, were demonstrated in C. asiatica leaves.

성경에 나타난 체육 -신체관, 스포츠, 여가관을 중심으로-

김옥태 ( Ok Tae Kim ) 기독교학문연구회 2012 신앙과 학문 Vol.17 No.2

이 논문은 신·구약 성경과 관련 문헌을 통하여 신체관, 스포츠, 여가관 등을 중심으로 성경에 나타나 있는 체육의 개념에 대해 고찰한 것으로서 결과는 대체로 다음과 같다. 첫째, 성경에서의 신체관은 피조의 영적 존재이다. 즉 인간은 하나님의 형상과 모양에 따라 창조된 육을 가진 존재이나 영혼도 소유하고 있다. 육체는 약하지만 무시할 것이 아니라 잘 관리할 책임도 있다. 일찍이 성경은 오늘날 체육의 개념인 심신일원론적이었다. 둘째, 성경에 나타난 스포츠는, 구약시대에는 주로 활, 칼, 창, 물매 등 전쟁과 관계있는 것과, 무용, 수영 등이 성행하였다. 신약시대에는 바울서신에 여러 비유로 나타나 있는데, 이로써 스포츠가 부정되지 않고 긍정적으로 평가되고 있다는 것을 알 수 있다. 셋째, 성경에서의 안식일은 인간의 일과 탐욕에 대한 규제와 하나님이 보인 만족이라는 측면에서 여가적인 의미를 찾을 수 있다. 축제는 하나님이 만든 일종의 놀이라 할 수있고, 특히 그리스도인은 예배를 통하여 축하와 기쁨과 자유를 누려야 하는데, 인간의 문화는 그것을 위축시키고 있다. 결국, 체육이 역사적으로 기독교에서 부정된 적이 많았지만, 성경에 나타나 있는 진정한 의미는 긍정적이며 오히려 권장되어야 하는 것으로 나타나 있는 것이다. This paper considered the concept of physical education in the Bible. The three topics presented were the view of the human body, sports and the view of leisure through the Bible. The results are as follows: First, the view of the body in the Bible, is that we are created by God as spiritual beings both in body and soul. Though the body is weak, we must not neglector the body, but we must manage it responsibily. In the Bible, one can identify similar ideas that are practiced in modern physical education, the Bible shares the same view as modern physical education. Second, in the Old Testament days, archery, fencing, spear and sling were all physical activities related to war, while dance and swimming were related to physical education and sports. In the New Testament days many metaphors are written in Pauline letters, it suggests that sports were not denied but were encouraged and venerated. Third, the Sabbath in the Bible encompasses the meaning of leisure, control of the human`s work ethics, greed and God`s satisfaction. Festivals are a sort of play God has made. Christians should enjoy celebrations, joy and freedom through worship, However human culture restricts these celebrations. Ultimately, though physical education has been denied in Christianity historically, the true meaning of physical education in the Bible is positive and encouraging message for all to practice and enjoy physical education.

노인의 여가스포츠 참가경험과 여가유능감이 삶의 질 만족에 미치는 영향

김옥태(Kim, Ok-Tae),이승희(Lee, Seung-Hee) 서원대학교 직지문화산업연구소 2011 湖西文化論叢 Vol.23 No.-

The purpose of this study is to provide data for promoting elders’ welfare by empirically analyzing the effects of elderly people’s experience of recreational sports participation and leisure competence on the satisfaction with quality of life, in order to improve their health and physical strength as well as to help them have mental, social, and emotional stability. One-way ANOVA and multiple regression analysis are applied to the study with 372 people of elderly participants and the results are as follows: First, according to the analysis of difference in the level of satisfaction with quality of life in terms of individual characteristics, there were significant differences in satisfaction depending on wealth and health conditions, not gender. Men had higher satisfaction with quality of life than women. The group with more wealth had higher satisfaction than the group with less wealth. The group with top level of health condition had the highest satisfaction, and then come the group with medium level of health condition and low level of health condition in order. Second, according to the analysis of difference in the level of satisfaction with quality of life in terms of experience of sports participation, there were significant differences in satisfaction depending on sports type, sports career, number of days of participation per week, and number of hours of participation per day. Competition sports type had the highest satisfaction, and then come rhythmic movement type, outdoor sports type, and training sports type in that order. The group with longer period of sports career had higher satisfaction than the group with shorter period of sports career. The group with more days of participation per week had higher satisfaction than the group with less days of participation per week. The group with more hours of participation per day had higher satisfaction than the group with less hours of participation per day. Third, according to the analysis on the effects of leisure competence of elderly participants in recreational sports upon satisfaction with quality of life, all the sub-variables of leisure competence have significant effects on all the sub-variables of satisfaction with quality of life. Consequently, acknowledging the fact that elderly people’s experience of recreational sports participation and their leisure competence contribute to the satisfaction with quality of life, both administrative authorities in charge of elders’ welfare and leisure sports instructors should make their best efforts with positive PR activities in encouraging elderly people to actively participate in recreational sports for their healthy life of later years.

연구논문 : 행동형척도의 타당성, 신뢰도 및 응답 편이성에 관한 연구; 개혁성향 척도를 중심으로

김옥태 ( Ock Tae Kim ),조성겸 ( Sung Kyum Cho ),송종현 ( Chong Hyun Song ),장윤재 ( Yoon Jae Jang ),박민규 ( Min Gue Park ) 한국조사연구학회 2012 조사연구 Vol.13 No.2

본 연구는 미디어 이용 및 커뮤니케이션 행위를 예측하는 데 있어 주요한 변인으로 주목받고 있는 개인의 성향(disposition) 중의 하나인 개혁성(innovativeness)에 초점을 두고, 어떤 형태의 질문문항이 개혁성을 측정하는 데 있어 응답의 타당성 및 신뢰성, 그리고 문항이해의 용이성 및 응답의 편의성을 높일 수 있는지 살펴보고자 하였다. 즉 기존의 태도와 행동의 관계 및 이들의 측정과 관련한 연구들을 바탕으로 개혁성향 척도를 평가적 태도로 묻는 문항과 구체적인 행동으로 묻는 문항으로 이분화하고, 그 공통점과 차이점을 분석함으로써 개혁성 측정문항의 정확성을 높일 수 있는 가능성을 탐구하였다. Regarding the growing concern for the problems in social survey, researchers have been interested in methodological examination of the anticipated benefits and problems of levels of scale in measurement . This study focuses on innovativeness as an individual`s disposition variable being used to predict the behavior in media and communication studies. This study explores the possibilities of increasing accuracy of measuring innovativeness by comparing a scale asking behavioral entities with a scale asking attitudinal entities.

현대 여가에 대한 윤리적 고찰

김옥태(Ok Tae Kim) 한국체육철학회 2006 움직임의철학 : 한국체육철학회지 Vol.14 No.4

초등학교 태권도 수련생의 참가동기와 학교생활만족 및 자아개념에 관한 연구

김옥태(Ohk Tae Kim),정근화(Kun Hwa Jung) 한국체육교육학회 2011 한국체육교육학회지 Vol.16 No.3

이 연구는 태권도 수련의 자아개념에 대한 가설적 인과모형을 통해 그 관계를 검증하고자 수행되었다. 즉, 참가동기, 학교생활만족, 자아개념을 설정하여 구조방정식모형을 이용한 가설적 구조모형 검증을 통해 잠재 변인들 간의 인과적 관계를 검증하였다. 조사대상의 모집단은 초등학교 태권도 수련생이었으며 이 연구에서는 충청도에 소재하고 있는 10곳의 초등학교 태권도 수련활동 참가자를 무선집락표집방법을 이용하여 최종적으로 사용된 유효 표본은 187명 이었다. 이상의 연구방법과 절차에 의해 다음과 같은 결과를 얻었다. 첫째, 개인적 특성에 따라 자아개념은 차이가 있다. 둘째, 초등학교 태권도 수련활동 참가자의 자아개념을 설명하기 위해 제안된 모형은 적합하였다. 셋째, 초등학교 태권도 수련활동 참가자의 자아개념에 영향을 미치는 요인들의 상대적 영향력은 참가동기, 학교생활만족 순으로 나타났다. The purpose of this study is to examine the relationship among variables by establishing Self-conceps model on the school life satisfaction of elementary school students who participate in Taekwondo training activities. By classifying the motive of participation as exogenous latent variable, and school life satisfaction as endogenous latent variable, this study investigates the causality among latent variables through the verification of the hypothetical structural model by use of Structural Equation Model. In this study, The population of this study is elementary schoolers who train on Taekwondo. Taekwondo-training students in elementary schools located in Choongchung province are sampled by use of Cluster Random Sampling method, and 187 students are finally selected as valid sample. With the above methods and procedures, the results of this study are as follows. First, there are differneces in Self-conceps according to individual qualities. Second, the initial structural model designed to explain Self-conceps of elementary schoolers participating in Taekwondo training was inappropriate for the analysis due to some measured factors of motive of participation. The revised structural model, is appropriate improper mesured factors. Third, the relative influence over the factors affecting Self-concepsof elementary schoolers training on Taekwondo is the greatest in motive of participation, and the school life satisfaction come after in order.

체육과 인간형성 : 그 가능성과 한계

김옥태(Ok Tae Kim) 한국체육철학회 2000 움직임의철학 : 한국체육철학회지 Vol.8 No.2

The purpose of this study is to consider the possibility and limitation of character formation in physical education through the review of related literatures. I mentioned first the object of character formation of physical education with a special emphasis on physical, mental, social factor, and considered the condition of character formation from two points of view-physical activity and personality, and the attitude in physical education. The conclusions of the possibility and limitation of character formation in physical education are as follows: First, heredity and environment are important factors for character formation and their roles have been argued. But, these two factors interact with complex forms and play an important role in human development. Second, we can take the character to a fixed limitation within the certain limits through the studying means and method at sport site. We can`t improve the physical fitness or motor skill, and mental and social factors which we expect above genetic limits. But, we can recognize the limits of that by the developmental and physiological limits, the limits of this by doing physical activity intentionally. Third, except sports site, the transfer of emotion and social character through physical activity, to actual life can`t be expected. But it is different by individuals. If we want the attitude learned from the sports site to be transferred to daily life, we should make special efforts for generalization; we should fix our eyes on the principle of similarity by `Identical Element Theory`.

뉴스이용과 책임귀인이 셧다운제에 대한 태도에 미치는 영향

김옥태(Kim, Ock Tae),손영준(Son, Young Jun),나은경(Na, Eunkyung) 한국게임학회 2013 한국게임학회 논문지 Vol.13 No.4

본 연구는 논란 속에 도입된 인터넷 셧다운제에 대한 태도에 미치는 요인들을 살펴보고자했다. 이에 게임 이용 빈도, 뉴스 이용정도, 게임의 효과에 대한 평가, 게임의 부정적 효과에 대한 원인 등이 셧다운제에 대한 동의에 미치는 영향을 살펴보았다. 셧다운제가 도입된 직후에 서울시민 496명에 대한 1대1 면접조사를 실시하여 온라인게임 규제제도인 셧다운제에 대한 태도를 살펴본 결과, 뉴스미디어를 더 많이 이용할수록, 게임의 부정적 영향을 더 많이 인식할수록, 그리고 게임의 부정적인 영향에 대한 책임이 정부에 있다고 인식할수록 셧다운제를 더 찬성하는 것으로 나타났다. 귀인이론을 중심으로 결과에 대한 논의와 연구의 함의를 제시했다. This study explored the relationships between media use and attributions of negative ffects of video games on attitudes toward Shut-down system. To prevent the youth from game addiction and secure a right to sleep for the youth, Shut-down system ahs been enacted since the fall of 2011. Interview survey revealed that females, the aged, those who use more news products, those who perceive more negative effects of games, and those who attribute the negative effects of game on government would have more positive attitudes toward the shut-down system.