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시호 모상근 배양에서 생장과 시호 사포닌 생합성에 미치는 배지와 IAA 및 IBA의 영향
안준철,김응식,이현진,황백,Ahn, Jun Cheul,Kim, Eung Sik,Lee, Hyun Jin,Hwang, Baik 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.3
시호 모상근 (BFHR2 클론) 배양에서 생장과 saikosaponin 생산에 미치는 기본배지, 배지의 다량무기염농도 변화 및 IAA와 IBA의 효과를 조사하였다. 조사한 배양액 중 RCM 배지에 다량무기염농도를 2배로 증가시킨 배지에서 생장이 가장 양호하였다. 또한 2RCM배지에서는 saikosaponin인 a, c와 d의 생성을 확인하였으며, 반면에 MS배지에서는 saikosaponin의 생성이 극히 저조하였다. IBA는 0.5mg/L에서 약 60%의 생장촉진효과를 보였으나, IAA와 IBA두 가지 모두 0.01~5mg/L 까지의 농도에서 뿌리형태의 외적인 변화 없이 saikosaponin 생성을 강하게 억제하는 작용을 나타내었다. We examined the effects of various basal media and exogenous auxins on the growth and saikosaponin production in hairy root cultures of Bupleurum falcatum. Of the various media tested, 2RCM medium was superior for growth and saikosaponin content, in which saikosaponin a, c, and d was produced, however MS medium was resulted in extremely low level in saikosaponin production. By the treatment of 0.5 mg/L IBA the growth rate was increased by about 60% in 2RCM medium, whereas both IAA and IBA showed a potent inhibition effect in saikosaponin biosynthesis by the treatment of 0.01 mg/L~5mg/L.
저널리즘 생산자로서 '문제적 기자'에 관한 연구 - 민속지학적 방법을 중심으로 -
안준철,김영찬 한국외국어대학교 국제커뮤니케이션연구소 2019 東西言路 Vol.45 No.-
The study began with a focus on social phenomena in which producers, journalists, are in the spotlight rather than articles, which are the result of journalism. After an era of exclamations, exclusive or in-depth articles, recent audience of journalism have begun to attack journalists. The behavior, which began from a critical perspective on the results of journalism produced by a particular journalist, came at last to attach the scornful term ‘Kireki(journalist+trash)’ to journalists. Many people are concerned about this social phenomenon as a ‘crisis of journalism’. Therefore, the crisis phenomenon of journalism was approached through in-depth interview with journalists who are considered to be representative 'problematic journalist' among journalists and news consumers. The ‘problem journalists’ had something in common that the way they entered the world of journalists was not orthodox and did not have a proper journalism education. They pretended to be a fanatic even before entering the world of journalist in the field they were covering, and after becoming a journalist, they were either overly friendly to what they liked or obsessed with comments advocating their own articles. However, the fact that they have become 'problematic journalists' could not simply turn it into an individual problem. The reality that the control of the newsroom has become meaningless due to changes in the media environment, and the excessive authority given to journalists' individuals have also made them problematic journalists.
시호(Bupleurum falcatum L.)의 모상근 배양을 통한 Saponin 생산 I. 캘러스, 부정근, 모상근과 재배근의 Saponin 양상과 함량 비교
안준철 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from these were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 $\mu$M 2, 4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes. A4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.