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      • KCI등재

        스포츠 빅이벤트에 대한 신문 사진 보도 분석 : 2020 도쿄올림픽을 중심으로

        안준철,이상욱 현대사진영상학회 2021 현대사진영상학회논문집 Vol.24 No.3

        The purpose of this study is to examine how the media handles and reproduces the Olympics by grasping the trend of photographic reporting at the Tokyo Olympics in daily newspapers, identifying topics and reports, and analyzing photographic images published in newspapers. It is also important to observe how our media reproduce by looking at the differences in media that appear through Olympic photo images. For this study, a total of 539 photos were analyzed by selecting press photos posted in general daily newspapers such as the Chosun Ilbo, the Hankyoreh Newspaper, and the Hankook Ilbo from July 23 to August 9, 2021. As a result of the analysis, the area average of the photos showed significant differences between media. In addition, in the case of a front-page article, the media to be analyzed reported more than one related photo during the Olympics. Even when the subject of photography was women, the degree of exposure differed from medium to medium. The main contents of the photo report were “physical athletic ability” and “the joy, pleasure and cheers of victory.” However, the Chosun Ilbo showed a lower percentage of reports related to COVID-19 than the other two media companies. The theme of all newspapers was “victory advantage.” However, the increase in the proportion of humanism is interpreted as meaning that the tendency to report gold-oriented past Olympics is changing.

      • SCOPUSKCI등재

        시호(Bupleurum falcatum L.)의 모상근 배양을 통한 Saponin 생산 I. 캘러스, 부정근, 모상근과 재배근의 Saponin 양상과 함량 비교

        안준철 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1

        In order to survey possibility to produce saikosaponin from in vitro hairy root culture, culture of callus, adventitous root, and hairy root of Bupleurum falcatum L. were estabilished, and quantitative and qualitative aspects in saikosaponin extracted from these were compared with those of cultivated root. Callus grew well in MS medium containing 0.9 $\mu$M 2, 4-D. In contrast, both of adventitous root and hairy root grew well in hormone-free MS medium. However, hairy root showed more rapid growth with extensive lateral root branches, characteristics of lower content of water and softer than in adventitous root. Among the selected lines of adventitous root and hairy root were observed difference in the growth rate. Mannopine, one of opine synthesized in the transformed tissue with Agrobacterium rhizogenes. A4 were detected in the extract of hairy root lines. Pattern and content of crude saponin from adventitous and hairy root showed no difference, but somewhat difference from those of cultivated root. However, in callus, distinct production-aspect of saponin was not observed.

      • KCI등재

        시호 모상근 배양에서 생장과 시호 사포닌 생합성에 미치는 배지와 IAA 및 IBA의 영향

        안준철,김응식,이현진,황백,Ahn, Jun Cheul,Kim, Eung Sik,Lee, Hyun Jin,Hwang, Baik 한국식물생명공학회 1999 식물생명공학회지 Vol.26 No.3

        시호 모상근 (BFHR2 클론) 배양에서 생장과 saikosaponin 생산에 미치는 기본배지, 배지의 다량무기염농도 변화 및 IAA와 IBA의 효과를 조사하였다. 조사한 배양액 중 RCM 배지에 다량무기염농도를 2배로 증가시킨 배지에서 생장이 가장 양호하였다. 또한 2RCM배지에서는 saikosaponin인 a, c와 d의 생성을 확인하였으며, 반면에 MS배지에서는 saikosaponin의 생성이 극히 저조하였다. IBA는 0.5mg/L에서 약 60%의 생장촉진효과를 보였으나, IAA와 IBA두 가지 모두 0.01~5mg/L 까지의 농도에서 뿌리형태의 외적인 변화 없이 saikosaponin 생성을 강하게 억제하는 작용을 나타내었다. We examined the effects of various basal media and exogenous auxins on the growth and saikosaponin production in hairy root cultures of Bupleurum falcatum. Of the various media tested, 2RCM medium was superior for growth and saikosaponin content, in which saikosaponin a, c, and d was produced, however MS medium was resulted in extremely low level in saikosaponin production. By the treatment of 0.5 mg/L IBA the growth rate was increased by about 60% in 2RCM medium, whereas both IAA and IBA showed a potent inhibition effect in saikosaponin biosynthesis by the treatment of 0.01 mg/L~5mg/L.

      • KCI등재

        미치광이풀 모상근 배양에서 적정 sucrose 농도 및 hydroxyapatite 첨가에 의한 tropane alkaloid 생산성 향상

        안준철,양선주,표병식,최지원,황백,An, Jun-Chul,Yang, Sun-Ju,Pyo, Byung-Sik,Choi, Ji-Won,Hwang, Baik 한국식물생명공학회 1998 식물생명공학회지 Vol.25 No.1

        미치광이풀(Socopolia parviflora Nakai) 모상근 배양에서 모상근 생장과 tropane alkaloid 생산에 대한 sucrose농도와 일부 흡착제의 효과를 조사하였다. 모상근 클론 SP 11은 5% sucrose 농도를 함유한 1/2 B5 배지에서 최적인 생장과 tropane alkaloid 생산을 보였다. 모상근에서 tropane alkaloids 생성양상은 모식물에서와 약간의 차이를 보였고 그 중 식물체에서 검출되지 않는 littorine의 생성이 모상근에서 두드러졌다. 조사된 흡착제(0.01% 1%) 중에서, charcoal 0.01%와 XAD-II l%에서 약간의 생장촉진효과를 보인 것을 제외하고는 그 외 농도나 흡착제(amberlita chitosan)에서는 억제효과 또는 유의적인 변화를 보이지 않았다. 반면에 hydroxyapatite는 미치광이풀 모상근의 생장과 tropane alkaloid 생산에 있어 촉진효과가 있음이 확인되었다. The effects of sucrose concentration and some absorbents on growth and tropane alkaloid production in hairy root cultures of Scopolia parviflora were investigated. The maximum effect on growth and tropane alkaloid production in hairy root clone SP11 was obtained for 1/2 B5 medium containing 5% sucrose. The production pattern of tropane alkaloid in hairy roots was some different from that of rhizome of mother plant, particulary showing high littorine contents, which was not found in ordinary roots. Among absorbents examined, charcoal 0.01% and XAD-II 1% made a slight growth promotion effect, whereas the other concentration of charcoal, XAD-II and absorbents (amberlite and chitosan) showed inhibition or no significant effect. The addition of hydroxyapatite enhanced the production of tropane alkaloids significantly than control cultures.

      • SCOPUSKCI등재

        미치광이풀(Scopolia parviflora)의 모상근 배양에 의한 Tropane Alkaloid 생산

        安埈徹,鄭炳均,白允雄,金永俊,高庚珉,黃聲振,황백 한국식물학회 1993 Journal of Plant Biology Vol.36 No.3

        Transformed hairy roots of Scopolia parviflora, prducing tropane alkaloids and native to Korea, were obtained following infection of rhizome segments with Agrobacterium rhizogenes A_4. Each root tip induced from inoculum sites excised and cultured in MS agar or liquid medium. About seventy of hairy root clones were established. Among them, several fast growing hairy root clones were examined for alkaloid content. Two dimensional TLC analysis showed that the tropane alkaloid pattern of hairy root was more complicated than that in the rhizome of mother plant. On the other hand, some hairy root clones did not produce scopolamine and hyoscyamine. In HPLC analysis, some hairy root clones yield higher levels of scopolamine and hyoscyamine than those of mother plant rhizome which used for infection. Scopolamine and hyoscyamine were identified by comparison of their retention times and of their mass spectra data with those of authentic compounds.

      • KCI우수등재

        시호 모상근 배양에서 배지성분에 따른 사이코사포닌 함량과 조직학적 형태의 차이

        안준철,정영재,이규배,김옥태,황백 한국약용작물학회 1999 韓國藥用作物學會誌 Vol.7 No.4

        시호 모상근 태양에선 MS와 RCM 배지를 기본배지로 하는 몇 가지 배지를 사용하여 생장과 사이코사포닌 함량을 비교하였으며, 그 중 MS와 3RCM 두 배지에서 보이는 생장과 성분 함량의 차이가 뿌리의 조직학적 차이와 연관이 있는지를 조사하였다. 모상근의 생장은 생중량은 MS배지가 건중량은 3RCM 배지가 각각 양호하였으며, MS와 3RCM의 건중량에 대한 생중량의 비가 각각 7.6%와 14%로 MS 배지가 함수량이 높았다. 사이코사포닌 함량은 MS배지에서는 극히 낮은 함량을 나타내었으나, 3RCM 배지에서는 총함량에서 국내 1년산 재배근의 약 2.2~5.5배의 함량을 나타내었다. 한편, 두 배지에서 배양한 시료의 근단부와 뿌리끝 1 cm 부근에서는 조직학적인 큰 차이를 관찰할 수 없었으나, 성숙대에서 는 MS 배지 보다 3RCM 배지에서 목부 사부 세포들의 발달이 보다 뚜렷하고, 3RCM 에서는 많은 세포에서 탄닌을 함유하는 것으로 추정되는 진한 염색의 액포 또는 세포 소기관이 관찰되었다. 따라서 이러한 세포 소기관의 발달은 사이코사포닌을 포함한 2차대사물질의 생합성과 연관이 았는 것으로 사료된다. Growth rate and saikosaponin content of Buplurum falcatum hairy root on MS and RCM basal media were measured. And using hairy root cultured in those media, it was investigated whether some correlation between differences in growth rate and saikosaponin content and histiological difference was or not. Results obtained showed that among media tested, MS and 3RCM medium which showed the faster growth in fresh weight and dry weight, respectively. And saikosaponin content of hairy root cultured in 3RCM was 2.2~5.5 times higher than that of 1 year cultivated roots, whereas that in MS was extremely low. On the other hand, histological differences of root tips and about 1 cm region from root tip cultured in both MS and 3RCM were not prominent, however, in mature region, a lot of cells only in 3RCM contained densely stained vacuoles or organelles which probably contain tannin, suggesting that development of those has a correlation with biosynthesis of secondary metabolites containing saikosaponin.

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