http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
인간 지방조직유래 줄기세포의 PLCL/피브린 복합지지체에서 연골세포로의 분화
김상희 ( Sang Hee Kim ),정영미 ( Young Mee Jung ),김상헌 ( Sang Heon Kim ),이종원 ( Jong Won Rhie ),김영하 ( Young Ha Kim ),김철근 ( Chul Geun Kim ),김수현 ( Soo Hyun Kim ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
In cartilage tissue engineering, choosing on an appropriate cell source and a scaffold for cartilaginous tissue formation seriously influences the clinical application of an engineered cartilage. The purpose of this study is to investigate chondrogenic differentiation of human adipose tissue-derived stromal cells(hASCs) by hybridizing of fibrin gels for mimicking 3-dimensional environments of native cartilage and a poly(L-lactide-co-ε-caprolactone)(PLCL) scaffold. PLCL scaffolds with 85% porosity and 300-500 ?m pore size were fabricated by a gel-pressing method. For examining chondrogenic differentiation of hASCs, cells were mixed with 0.5% fibrin solutions and subsequently they were seeded onto PLCL scaffolds. After that, cell-scaffold constructs were implanted subcutaneously in nude mice for up to 8 weeks. Specimens were harvested after 8 weeks and analyzed. From in vivo studies, the constructs used with fibrin-PLCL hybrid scaffolds showed more depositions of cartilage-specific ECM components, such as GAGs and collagen type II, as compared to the constructs used with PLCL scaffolds alone. Furthermore, the constructs seeded with hASCs that were induced to chondrogenesis in vitro prior to implantation improved cartilage matrix deposition as compare to the constructs seeded with non-induced hASCs. These results indicated that the use of the fibrin gels-PLCL hybrid scaffold could enhance chondrogenic differentiation of hASCs and cartilaginous tissue formation. Also, the induction for chondrogenesis of hASCs prior to implantation in vitro could affect cartilaginous tissue formation in vivo. In conclusion, the hybrid system which was fabricated with the inoculation of ASCs and fibrin gels onto a PLCL scaffold could be a meaningful system in cartilage tissue engineering.
일개 상급종합병원 의료인의 감염관리 지침 준수 의도 영향요인: 계획된 행위이론을 중심으로
최영신(Choi, Young Sin),김수현(Kim, Su Hyun),장현하(Chang, Hyun Ha) 기본간호학회 2021 기본간호학회지 Vol.28 No.4
Purpose: This study aimed to identify factors affecting physicians and nurses’ intention to comply with healthcare-associated infection control guidelines, based on the theory of planned behavior. Methods: This study was a cross-sectional, descriptive survey. Data were collected using self-report questionnaires from 169 doctors and nurses at a tertiary hospital. Pearson correlation analysis and multiple regression analysis were used to analyze the factors influencing intention to comply with healthcare-associated infection control guidelines. Results: The mean score of intention to comply with infection control guidelines was 17.17±2.60 points (possible range 3-21 points). The intention to comply with infection control guidelines was positively associated with subjective norms and perceived behavioral control over compliance with healthcare-associated infection control guidelines. In the multiple regression analysis, perceived behavioral control was the only significant predictor of intention to comply with healthcare-associated infection control guidelines. Conclusion: In order to encourage doctors and nurses to comply with infection control guidelines, it is necessary to provide resources, enhance the work environment, and utilize strategies to increase perceived behavioral control over compliance with infection control guidelines.