인지능력 개선 응용기술 및 프로바이오틱스 적용 식품 소재 개발

정재연,김세헌,신용국,오남수,Joung, Jae Yeon,Kim, Sae Hun,Shin, Yong Kook,Oh, Nam Su 한국축산식품학회 2018 축산식품과학과 산업 Vol.7 No.1

프로바이오틱스 섭취를 통한 알코올성 간 질환의 완화

이인옥 ( In Ok Lee ),김세헌 ( Sae Hun Kim ) 한국유가공기술과학회 2014 Journal of Dairy Science and Biotechnology (JMSB) Vol.32 No.1

Probiotics have been extensively studied for their beneficial effects on human health. In particular, Lactobacillus and Bifidobacterium strains have gained considerable attention as major groups of probiotic bacteria that improve gastrointestinal health. However, emerging evidence suggests that probiotics offer benefits beyond those observed in the gut recent studies suggest that probiotics and/or their components exert favorable effects on alcoholic liver disease (ALD) pathogenesis such as decreasing intestinal permeability, inhibiting pathogenic bacteria growth, increasing the activity of alcohol metabolism enzymes, modulating the adaptive immune system, and suppressing fatty acid synthesis genes. In this review, we discuss the results of in vivo and in vitro studies that have examined the use of probiotics to prevent ALD, primarily focusing on those that explore the cellular and molecular mechanisms underlying the activities of promising probiotic strains. The evidence presented in this review could help in screening for probiotic strains that have protective effects in ALD patients and in further elucidating the mechanisms of their actions.

시판 생막걸리에서 분리한 유산균의 프로바이오틱스 기능성 연구

정상은(Sang-Eun Jung),김세헌(Sae-Hun Kim) 한국식품과학회 2015 한국식품과학회지 Vol.47 No.1

본 연구에서는 시판 생막걸리에서 분리한 유산균의 probiotics 특성을 연구하기 위해 내산성, 내담즙성, 장 부착능에 효능이 있는 6개 균주를 선별하여 동정하였다. 생막걸리에서 분리한 유산균을 pH 2.5의 산성 및 담즙산(0.3% oxgall) 조건에서 내산성과 내담즙성을 측정한 결과 BSM-2, BSM-3, GSM-3, TJH-1, EHJ-1, EHJ-2의 6개 균주에서 각각 107, 108 CFU/mL 높은 생존률을 나타내어 우수한 내산성과 내담즙성을 확인할 수 있었다. 내산성과 내담즙성에 효능이 있는 6개 균주의 16S rDNA 분석 결과, L. plantarum (BSM-2, EHJ-1) L. casei (GSM-3, EHJ-2), L. brevis (BSM-3), P. pentosaceus (TJH-1)의 4개 group이 동정되었다. 장부착능에서는 다른 유산균에 비해 장내 부착능이 뛰어난 것으로 알려진 L. rhamnosus GG를 대조군으로 사용하였으며 HT-29 cell에 대해 6종의 유산균이 모두 장 부착능을 보였으며 L. casei (GSM-3, EHJ-2)가 가장 장 부착능이 높았다. 항산화능력은 L. plantarum BSM-2와 EHJ-1에서 각각 32.44%, 24.65%의 DPPH radical 소거능을 보였다. 시판 생막걸리에서 분리된 유산균은 다양한 식중독 원인 세균에 대하여 항균활성을 지니고 있었으며, 본 연구에서는 유산균이 생성하는 항균물질인 bacteriocin이 아닌 유산균 발효 중 젖산을 생성하면서 pH의 저하로 항균활성을 보인다는 결과를 확인할 수 있었다. Probiotics로의 기능성을 가진 6개 균주의 콜레스테롤 저하능력은 L. plantarum BSM-2와 EHJ-1에서 각각 68.82%, 56.38%의 높은 감소율을 나타내었다. 시판 생막걸리에서 분리한 유산균의 probiotic 특성을 조사해 본 결과 L. plantarum BSM-2, EHJ-1, L. brevis BSM-3, L. casei GSM-3, EHJ-2, P. pentosaceus TJH-1 모두 프로바이오틱스의 기본특성을 지니고 있어 프로바이오틱 균주로서 이용가치가 충분하다고 판단되어지며, L. plantarum BSM-2가 내산성, 내담즙성이 가장 우수한 결과를 나타내었고, 장 부착성이 우수하였으며, 뛰어난 항산화 능력과 항균력을 지니며, 콜레스테롤 저하능도 뛰어나 가장 우수한 프로바이오틱 균주라고 판단된다. The purpose of this study was to characterize the lactic acid bacteria found in makgeolli in terms of bacterial identity and gastric compatibility. Lactic acid bacteria were isolated from commercial raw makgeolli and separated into six strains that are resistant to gastric acidity and bile acid. These strains were identified by analysis of their 16S rDNA, as Lactobacillus plantarum BSM-2 and EHJ-1, Lactobacillus casei GSM-3 and EHJ-2, Lactobacillus brevis BSM-3 and Pediococcus pentosaceus TJH-1. All strains exhibited adhesion to intestines, showing that they were probiotic. We also found that L. plantarum BSM-2 had excellent resistance to bile acid as well as antioxidant activity. Taken together with its antibacterial properties and ability to lower cholesterol, our data suggest that L. plantarum BSM-2 was the most beneficial probiotic among the six strains.

후산 발효 적합 균주 선발 및 특성

손지양 ( Ji Yang Sohn ),김세헌 ( Sae Hun Kim ) 한국유가공기술과학회 2013 한국유가공기술과학회지 Vol.31 No.1

Emerging studies suggest that vegetables or fruit juices deemed to be potential alternative base medium for lactic acid bacteria fermentation. Until now, limited studies have been carried out to evaluate such applications. Thus, the objective of present study is that lactic acid bacteria were evaluated for their viability at low pH, growth during storage at low temperature, and CO2 formation. Furthermore, the effects of grapefruit extract with respect to cell viability, sensory ability, and organic acid production were evaluated for these strains. The probiotic properties of the strains, including acid tolerance, bile tolerance, and adhesion to human intestinal epithelial cells (HT-29 cells), prebiotic characteristics, and safety features were examined. All strains survived in MRS medium broth adjusted to pH 3.8, at 10℃ for 6 days, and did not produce CO2 to check post fermentation. The medium of grapefruit extract fermentation by Lactobacillus plantarum CJIH 203 resulted in maximal viable counts, compared with other strains, and the extract subsequently tasted sour due to the presence of lactic acid. Lactobacillus plantarum CJIH203 was highly resistant to artificial gastric juice and intestinal juice, while Lactococcus lactis SJ09 strongly adhered to HT-29 cells. Tagatose showed the greatest ability to enhance the growth of L. plantarum SJ21, relative to the other strains. All strains were verified by safety tests such as hemolysis, gelatin hydration, and urea degradation. Therefore, these strains could be promising candidates for use in reducing excessive post-fermentation and functional products.

향신료의 유산균 생장 촉진 효과와 홍고추(Capsicum annuum L.) 발효 산물의 HT-29 세포에서의 Tight Junction Protein 유전자 발현 조절 확인

김지현(Ji Hyun Kim),신민재(Min Jae Shin),정명준(Myung June Chung),김세헌(Sae Hun Kim) 한국식품영양과학회 2021 한국식품영양과학회지 Vol.50 No.1

본 연구에서는 한식에서 많이 사용되는 마늘, 양파, 생강, 고추, 청양고추 등 향신료에서 한국 사람으로부터 분리된 Lactobacillus 및 Bifidobacterium 등 10종(한국 영유아 분변 유래 4종, 한국 성인 분변 유래 5종, 김치 유래 1종)의 유산균이 높은 생장성을 보이는 것을 확인하였으며, 8개의 유산균이 5가지의 향신료 중 홍고추 수용성 추출물에서 가장 높은 생장성을 나타냈다. 실험 균주의 프로바이오틱스로서의 특성을 파악하기 위해 장 상피세포인 HT-29에 대한 부착능력, 내산성 및 내담성, 젤라틴 분해효소 및 요소 분해효소의 활성을 시험하였다. 또한, 실험 균주를 홍고추 수용성 추출물에서 24시간 배양하여 DPPH 라디칼 소거능을 통해 유산균의 항산화 효과를 평가하였다. 그 결과, 향신료에서의 생장성이 높고 프로바이오틱스로서의 우수한 특성을 나타낸 B. bifidum CBT BF3, B. longum CBT BG7, L. acidophilus CBT LA1, L. plantarum CBT LP3 균주 4가지를 선별하고, 향신료 추출물 배양액 중에서 높은 항산화 효과를 보였던 생강과 홍고추를 선정하여 세포실험을 진행하였다. LPS로 염증이 유도된 HT-29 세포에 선별된 균주의 향신료 추출물 배양액을 처리하여 밀착연접 단백질인 ZO-1 및 Occludin의 mRNA 발현량이 증가함을 확인하였다. 이는 홍고추 추출물 배양액이 높은 항산화 기능성을 보일 뿐만 아니라 장벽 기능을 향상시킬 수 있는 가능성이 있음을 시사하고 있다. Spices are known to possess antibacterial activity against pathogens. In contrast, they also promote probiotic bacterial growth, such as Lactobacillus and Bifidobacterium. In the current study, 10 strains isolated from Korean human feces and kimchi were screened for bacterial growth in the presence of spices mainly used in Korean foods, including garlic, onion, ginger, red pepper, and Chungyang pepper. Majority of the strains showed maximum growth in red pepper. In order to investigate properties of the candidate strains as probiotics, we evaluated the adhesion ability to intestinal epithelial HT-29 cells, acid and bile tolerance, and inhibition activity of gelatinase and urease. The functionality of the fermented product was subsequently studied, as most strains grew well in the presence of spices. The antioxidant activity was determined using DPPH (1,1-diphenyl-2-picrylhydrazyl). Fermented products of ginger and red pepper with strains showed higher free radical scavenging activity than the spices themselves. In addition, subsequent to lipopolysaccharides-induced impairment, the fermented products of red pepper with selected strains also exhibited increased mRNA expression of tight junction protein in HT-29 cells. Taken together, the results of this study indicate that strains isolated from Korean human feces and kimchi have a high growth rate in the presence of spices, and their fermented products exert increased anti-oxidant activity, thereby indicating the potential functionality in improving gut barrier functions.

식품공정환경에서의 Listeria monocytogenes의 바이오필름

윤현선 ( Hyun Sun Yun ),김세헌 ( Sae Hun Kim ),전우민 ( Woo Min Jean ) 한국유가공기술과학회 2009 Journal of Dairy Science and Biotechnology (JMSB) Vol.27 No.2

Listeria monocytogenes is a major concern in food processing environments because it is ubiquitous and can easily contaminate food during processing. Contaminated food and the surfaces in food facilities can serve as reservoirs of L. monocytogenes, which can lead to the serious foodborne illness listeriosis in consumers. L. monocytogenes can adhere to materials commonly used in food processing equipment and form biofilms. In the biofilm mode, L. monocytogenes is significantly more resistant to disinfection or sanitizers than its planktonic counterparts. Many researchers have studied the effects of surface materials on bacterial adhesion and the formation of biofilms. Recent studies have focused on preventing the establishment of L. monocytogenes in niches in the food plant environments.

κ-Casein의 Chymosin, Pepsin 및 Trypsin 가수분해물에 대한 안지오텐신 변환효소 저해효과의 탐색

오세종(Se-Jong Oh),김세헌(Sae-Hun Kim),김상교(Sang-Kyo Kim),백영진(Young-Jin Baek),조경현(Kyung-Hyun Cho) 한국식품과학회 1997 한국식품과학회지 Vol.29 No.6

만성 스트레스성 장 질환 유래 수면 장애 및 뇌 기능 저하 개선 기능성 유제품의 개발 동향

정재연(Jae Yeon Joung),김경수(Kyeong Soo Kim),김세헌(Sae Hun Kim),김형욱(Hyung Wook Kim),박광심(Gwang Sim Park),강신호(Shin Ho Kang) 한국축산식품학회 2021 축산식품과학과 산업 Vol.10 No.2

알 및 알가공품의 유통기한 설정을 위한 지표개발 및 산출 연구

안지희,박정민,구자경,윤수진,이정수,김장미,김세헌,김영주,박재우,송성옥,위성환,김진만,An, Ji-Hui,Park, Jung-Min,Gu, Ja-Gyeong,Yoon, Su-Jin,Lee, Jeong-Soo,Kim, Jang-Mi,Kim, Sae-Hun,Kim, Ryong-Joo,Park, Jae-Woo,Song, Sung-Ok,Wee, Sung-Hwan 한국축산식품학회 2012 한국축산식품학회지 Vol.32 No.3

본 연구는 국내에서 유통되고 있는 식용란 및 전란액의 품질지표설정을 통하여 유통기한을 산출하기 위해 수행하였다. 식용란은 및 전란액(살균, 비살균)에 대한 저장기간별 저장온도에 따른 미생물학적, 이화학적, 관능적 특성을 측정하였다. 품질지표 개발을 위해 저장기간별 저장온도에 따라 식용란은 세균수, 대장균군, Salmonella sp., VBN, pH, 산가, Haugh unit을 품질지표로 설정하여 측정하였으며, 전란액은 Haugh unit을 제외하여 측정하였다. 유통기한은 품질지표 중 가장 먼저 품질한계에 도달한 성상(관능)을 근거로 하여 $10^{\circ}C$에서는 42일, $15^{\circ}C$에서는 27일,$25^{\circ}C$에서는 9일, $35^{\circ}C$에서는 2일, $45^{\circ}C$에서는 1일로 산출하였다. 전란액은 품질지표 중 가장 먼저 품질한계에 도달한 세균수를 근거로 하였으며, 살균전란액은 $10^{\circ}C$에서는 7일, $15^{\circ}C$에서는 3일, $25^{\circ}C$에서는 2일, $35^{\circ}C$에서는 1일, $45^{\circ}C$에서는 1일 미만으로 산출하였고 비살균전란액은$10^{\circ}C$에서는 4일, $15^{\circ}C$에서는 2일, $25^{\circ}C$에서는 1일, $35^{\circ}C$와 $45^{\circ}C$에서는 1일 미만으로 유통기한을 산출하였다. 결론적으로 저장기간과 가장 높은 상관관계를 가진 온도의 품질 한계일을 유통기한으로 산출하였다. 따라서 식용란은 27일, 살균전란액은 7일, 비살균전란액은 4일로 유통기한을 산출하였다. The study was conducted to determine bio-markers and establish shelf-life for eggs and egg products. The selected biomarkers were measured storage period according to samples (two months for table eggs and two weeks for whole liquid eggs) and five storage temperatures ($10^{\circ}C$, $15^{\circ}C$, $25^{\circ}C$, $35^{\circ}C$ and $45^{\circ}C$). The bio-markers for table eggs determined pH, acid value, VBN (volatile basic nitrogen), HU (Haugh unit), aerobic plate counts, coliform group, and Salmonella sp. The bio-markers for whole liquid eggs excluded HU in the bio-markers of eggs. The shelf-life of table eggs observed as 42 d at $10^{\circ}C$, 27 d at $15^{\circ}C$, 9 d at $25^{\circ}C$, 2 d at $35^{\circ}C$, and 1 d at $45^{\circ}C$ in sensory overall acceptability. The shelf-life of pasteurized whole liquid eggs observed as 7 d at $10^{\circ}C$, 3 d at $15^{\circ}C$, 2 d at $25^{\circ}C$, 1 d at $35^{\circ}C$, and less than one d at $45^{\circ}C$ in total plate count. The shelf-life of non- pasteurized whole liquid eggs observed as 4 d at $10^{\circ}C$, 2 d at $15^{\circ}C$, 1 d at $25^{\circ}C$, and less than 1 d at $35^{\circ}C$ and $45^{\circ}C$ in total plate count.

프로바이오틱스 섭취를 통한 염증성 장 질환의 완화

이진 ( Jin Lee ),윤현선 ( Hyun Sun Yun ),김세헌 ( Sae Hun Kim ),전우민 ( Woo Min Jeon ) 한국유가공기술과학회 2010 Journal of Dairy Science and Biotechnology (JMSB) Vol.28 No.2

For centuries, probiotics have been known to promote health and prevent diseases. In recent times, modulation of diseases related to the immune function by probiotics has been recognized as very important to the health of the host`s gut. Inflammatory bowel diseases (IBDs) are the most frequently studied diseases in which probiotic administration has been tested as a potential therapy. Various in vitro and in vivo studies have been performed. The studies discussed in this review suggest several mechanisms: probiotics could modulate the gut microflora by competing with disease-causing pathogenic bacteria and could directly regulate the mucosal immune system, which activates the innate and adaptive immune systems. In addition, human clinical trials have shown alleviation of disease symptoms of ulcerative colitis (UC), Crohn`s disease, etc. This study aimed to understand the molecular mechanisms underlying immune modulation by probiotics and review studies on the functional aspect of IBD alleviation by probiotics. With more scientific studies confirming the effect of probiotics, this therapy holds promise for use in alternative medicine and/or pharmaceutical preparations, given the long history of safe consumption of probiotics.