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RISS 인기검색어
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- 저자 : 김성건 ( Sung Kun Kim ) (검색결과 2 건)
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김성건 ( Sung Kun Kim ),안기훈 ( Ki Hoon Ahn ),이용호 ( Yong Ho Lee ),김탁 ( Tak Kim ),김선행 ( Sun Haeng Kim ) 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.12
목적 : 기저 난소용적이 성선자극호르몬 자극에 대한 난소반응성과 체외수정술 (IVF-ET)의 결과의 예측에 적절한 지표가 될 수 있는지 알아보았다. 난소 잠재력은 체외수정술의 임상적 성공과 밀접한 연관성이 있으므로, 자극 전에 잠재력을 예측하면 각각의 보조생식술 (ART)환자에게 상담하고 치료방법을 결정하는데 유용할 것이다. 연구 방법 : 2000년 1월부터 2001년 6월까지 본원에서 과배란유도를 통하여 체외수정 첫 주기를 시행받은 68명의 불임여성을 Objective : To find out whether the determination of the baseline ovarian volume would be a suitable predictor for ovarian response to gonadotropins stimulation and the outcome of IVF-ET. As the ovarian reserve is closely related with the clinical success
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Cumulus Free 생쥐 성숙란의 초자화 동결-융해 후 Simple Media에서의 수정 및 배 발달
정수경,김성건,이정재,오지현,이용호,김선행,Jung, Soo-Kyung,Kim, Sung-Kun,Lee, Jung-Jae,Oh, Ji-Hyun,Lee, Yong-Ho,Kim, Sun-Haeng 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.3
Objective: To observe the capability of fertilization and embryo development including blastocyst formation of the oocytes in simple media after thawing of the cryopreserved cumulus-free mouse oocytes by vitrification method. Methods: Oocytes were collected from 5 to 6 weeks old ICR female mice, and were denuded from the cumulus cells by 0.1% hyaluronidase. Recovered mature oocytes in study group were cryopreserved by vitrification method using EM grid for $5{\sim}7$ days. In brief, oocytes were exposed in dPBS containing 1.5 M EG and 5.5 M EG+1 M sucrose for 2.5 minutes and 20 seconds each, and then executed vitrification by plunging in LN2 after loading on EM grid. Thawing treated by exposure of 1, 0.5, 0.25 and 0.125 M sucrose solution for 2.5 minutes each in order and used for experiments. Spermatozoa aspirated form the epididymis of 12 weeks old ICR male mice were used for insemination after capacitation. T6 media containing 0.4% BSA were used for fertilization and development. Results: Survival and fertilization rates after thawing were 76.9% and 79.6% respectively. Fertilization rate was lower (p<0.005) than that of control group (92.9%). There was no difference in embryo developmental rates from 2-cell to morula, however, the blastocyst formation rate and mean cell numbers of blastocysts in study group (63.3%, $58.9{\pm}9.2$) were lower compared with those of control group (76.1%, $63.5{\pm}8.9$). Conclusion: Vitrification is an effective method for mouse mature oocyte cryopreservation with high survival and fertilization rate after thawing. And in simple media, fertilization rates and embryo development of frozen-thawed mouse oocytes are satisfactory.
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