알코올 섭취 유무에 따른 cyclohexane의 폐 독성

김병렬,이상희,조현국,Kim, Byung-Ryul,Lee, Sang-Hee,Cho, Hyun-Gug 한국현미경학회 2005 Applied microscopy Vol.35 No.2

주류의 섭취가 산업화학물질의 생체 내 독성유발에 어떠한 영향을 미치는지를 검초하기 위해 흰쥐를 이용하여 15% 에탄올을 6주간 섭취시킨 후, cyclohexane (CH)을 2일 간격으로 4회 복강 투여하고 24시간 후 다음과 같은 결과를 얻었다. 체중 당 폐 무게와 폐 세척액 내 단백질 함량은 에탄올 전처치군이 대조군과 비교하여 증가되었고, 폐 조직 중 glucose-6-phosphatase의 활성은 감소되었다. 형태학적 변화에서도 CH만 투여한 군과 비교하여 에탄올 6주 섭취 후 CH를 투여한 군에서 부분적인 무기폐현상과 색전현상이 심화되어 나타났다. 따라서 CH 투여 시 에탄올을 전처치함으로써 간 조직 중 CH 효소의 활성이 증가되어, 과잉의 대사부산물들이 폐에 분포됨으로 인해 폐 조직의 손상이 심화되는 것으로 나타났다. To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56 g/kg body weght), which had been ingested 15% ethanol for up to 6 weeks, 4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and lung tissues were collected during 24 hours prior to sacrifice of animals. Comparing with the control group, the lung weight per body weight (%) and the protein content in bronchoalveolar lavage fluid were increased in the ethanol-pretreated group, and the glucose-6-phosphatase activity in lung tissues was decreased in the CH-treated group. In a morphological observations, pulmonary embolus were found in the CH-treated group, whereas a partial pulmonary atelectasis and a much increase in pulmonary embolus were shown in the CH-treated group after pretreated with ethanol for 6 weeks. In conclusion, these results indicate that ethanol pretreatment could enhance CH metabolism and that CH treatment with ethanol pretreatment could induce lung injury due to the increased CH metabolism.

알코올 전처치한 흰쥐에 Cyclohexane 투여로 인한 간 세포 내 소포체 미세구조의 변화

김병렬,윤종국,조현국,Kim, Byung-Ryul,Yoon, Chong-Guk,Cho, Hyun-Gug 한국현미경학회 2006 Applied microscopy Vol.36 No.4

주류의 섭취가 산업화학물질의 생체 내 독성유발에 어떠한 영향을 미치는지를 검토하기 위해 흰쥐를 이용하여 15%에탄올을 6주간 섭취시킨 후, cyclohexane(CH)을 2일 간격으로 4회 복강 투여하고 24시간 후 다음과 같은 결과를 얻었다. CH의 투여로 인해 정상군과 비교하여 간 무게와 헐청내 xanthine oxidase활성은 증가되었고, 간 내 glucose-6-phosphatase활성은 감소되었다. 그리고 CH 대사효소인 cytochrome P450 dependent aniline hydroxylase와 alcohol dehydrogenase의 활성은 CH투여 후 유의적으로 증가되었으며, 알코올 전처치 후 CH투여군이 가장 높은 활성을 보였다. 미세구조적으로도 알코올 섭취군과 CH투여군 모두에서 조면소포체로부터 활면소포체로의 전환이 일어났으며, 전 실험군 중 알코올 전처치 후 CH투여군이 가장 높은 전환율을 보였다. 이러한 결과들로 볼 때, 병변을 초래하지 않는 수준의 알코올의 섭취는 CH대사활성을 증대시키는 것으로 판단되었다. To evaluate the effects of ingestion of alcoholic drinks on the toxicities of industrial compounds, cyclohexane (CH) was intraperitoneally administrated to rats (1.56g/kg body weight), which had been ingested 15% ethanol for up to 6 weeks,4 times by once a day and every other day. Following the last treatment of ethanol or CH, blood and liver tissues were collected after 4 hours prior to sacrifice of animals. By the injection of CH, liver weight (% of body weight) and xanthine oxidase activity in serum were increased, and glucose-6-phasphatase (G6P) activity in liver was decreased compared to them of control group. The activities of CH metabolizing enzymes, such as cytochrome P450 dependent aniline hydroxylase (CYPdAH) and alcohol dehydrogenase (ADH), were significantly increased by injection of CH, and those activities were the highest in CH-injected group after pretreated with alcohol. Ultrastructurally. both of alcohol treatment and CH injection induced transforming into the smooth-endoplasmic reticulum from rough-endoplasmic reticulum, the those rate was the highest in case of CH-injection after pretreated with alcohol. From these results, it is suggested that alcohol intake on a level without alcoholic degeneration of hepatocytes could enhance the CH metabolism of liver.

Influenza Virus Type A 감염 MDCK 세포에 있어서 Glutathione S-transferase의 활성 변동

김병렬(Byung-Ryul Kim),박남표(Nam-Pyo Park),윤종국(Chong-Guk Yoon),전태원(Tae-Won Jeon),이상일(Sang-Il Lee) 대한의생명과학회 2000 Biomedical Science Letters Vol.6 No.1

농도와 기간에 따른 바이러스 감염 및 항바이러스제인 amantadine 병행 첨가 시 Maddin-Darby canine kidney (MDCK) 세포 내의 free radical 해독계 효소인 glutathione S-transferase (GST)와 lactate dehydrogenase (LDH)의 활성 변동을 상호비교 관찰하였다. 바이러스에 감염된 MDCK 세포는 감염 3일 후 1 TCID?? 군은 80% 이상, 10 TCID?? 군은 거의 대부분의 단층세포가 탈락되는 병변 효과가 나타났다. Amantadine cytotoxic dose %가 증가함에 따라 MDCK 세포 내의 GST 및 LDH 활성은 농도에 따라 유의하게 감소되었고, 감염배지 내 LDH 활성은 대조군 보다 유의한 증가를 보였다. 인플루엔자 바이러스 type A 접종농도와 기간에 따른 MDCK 세포 내의 GST 및 LDH 활성은 1 및 10 TCID?? 감염군에서 감염 3일 후부터 유의하게 감소되었고, 감염배지 내의 LDH 활성은 10배 이상 증가되었다. 인플루엔자 바이러스 type A 100 TCID?? 감염과 amantadine 병행 첨가 시 GST 및 LDH 활성은 바이러스만 감염한 군 보다 MDCK 세포 내에서 대조군에 비하여 감소율이 낮았고, 감염배지 중 LDH 활성 역시 증가율이 낮았다. 또한 바이러스 감염 후 amantadine 90 ㎍/㎖ 첨가 시에 세포 내와 감염배지 중에서 가장 낮은 감소와 증가를 나타냈다. This study was performed to evaluate the activities of glutathione S-transferase (GST) and lactate dehydrogenase (LDH) in Maddin-Darby canine kidney (MDCK) cells infected with virus and/or treated with amantadine. On cell morphological findings, monolayer fractions in MDCK cells infected with virus were exfolated more than 80% in 1 TCID?? group and that in 10 TCID?? were completely exfolated after 3 days during infectious process. In proportion to the dose of amantadine, activities of GST and LDH of MDCK cells were significantly decreased and those of LDH in medium fraction were more significantly increased compared with control. According to in both dose and time of virus innoculation, activities of GST and LDH in MDCK cells were significantly decreased in 1 and 10 TCID?? infected cells after 3 days. LDH activities in infectious medium were remarkably rised at 10 fold. In case of the cell line inoculated with type A 100 TCID?? and additionally treated with amantadine, the decreasing rate to the control in activities of GST and LDH was lower than that in those in case of that infected with virus only. These results suggested that virus infection and amantadine treatment may effect the activity of the detoxicating enzyme in the target cells.

포스너의 법실용주의와 사회법에서 대법원의 법해석론

김병렬(Byung Ryul Kim),류승호(Seung Ho Lyoo) 서울대학교 법학연구소 법이론연구센터 2023 기초법학연구 Vol.- No.2

제네바諸協約과 戰時指揮官의 責任

金柄烈(Byung-Ryul KIM) 大韓赤十字社 人道法硏究所 2003 人道法論叢 Vol.- No.23

중소도시 주거지역과 상업지역의 기후요소 실측 연구

윤재옥(Yoon Jae-Ock),김병렬(Kim Byung-Ryul) 대한건축학회 2008 대한건축학회논문집 Vol.24 No.11

This phenomenon ? heat island effect ? used to be confined to the largest cities in Korea, especially Seoul. However, recently this phenomenon is starting to occur in even small and medium-sized cities which are also developing rapidly. In order to analyze the heat Island Effect in Cheon-an City, measurement points were selected in commercial areas and residential areas. We measured by continuous observation and recording temperature and humidity during 13days during the fall/middle season(2007.10.10-2007.10.22), and conducted measurements for 24days during the winter season(2008.1.15-2008.2.7). We selected 4 measurement points: the main street in the commercial central area, the Bus-Terminal, at a water-space, and at a park in the commercial area. Those points selected differed in terms of ground cover. We conducted our measurements using the instantaneous method. The instantaneous measurement was used during 4 time zones and at 4 different points. The measurement targets were temperature, humidity, air velocity. We also added surface temperature as another instantaneous measurement target. We analyzed and compared the measurement result values of the 4 points. According to our results, the temperature at the water space was the lowest during all measurement times. The highest temperature was recorded on the main street which was made of asphalt. The air velocity at the park was the lowest during all times and the temperature at the park was highest during the early morning.

뚝도水源地 第4工場 傾斜板沈澱池의 槪要와 그 効果測定에 관하여

張學淳(Chang Hak Soon),金秉烈(Kim Byung Ryul) 대한토목학회 1972 대한토목학회지 Vol.20 No.1

중소도시의 하절기 열환경 특성 연구

남선영(Nam Sun-Young),김병렬(Kim Byung-Ryul),윤재옥(Yoon Jae-Ock) 대한건축학회 2008 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.28 No.1(계획계)

Recently, the city is growing up rapidly as the population increases. The commercial and residential area of the city are sharply increased. As a result, the city appearing climate change present situation from artificial thermal and air pollution of material. Therefore, to analyze the phenomena of climate change in cities, it is needed that we should carry out research on the characteristics of 'flow and thermal environment' of small and medium size cities. Continuous measurement, instantaneous measurement carry out for more analysis thermal environment by climate change of Asan city. The continuous measurement selected the city center commercial areas and the residential areas and it measured a temperature, a humidity and a wind speed.(2008.08.05~2008.08.08) The instantaneous measurement selected crossroad, green space, water-space for measure time zone. (2008.08.07) We analyzed the data of continuation measurement and instantaneous measurement and the data of the same day at Korea meteorological observation station near by that city.

대학캠퍼스 외부공간의 가로등 설치에 관한 LCC분석 연구

주재성(Joo Jae-Sung),김호영(Kim Ho-Young),김병렬(Kim Byung-Ryul),정종림(Jung Jong-Rim),윤재옥(Yoon Jae-Ock) 대한건축학회 2007 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.27 No.1

This study evaluates the economic efficiency of road lamps in the University Campus with a Life-cycle cost(LCC) technique. In order to determine the most suitable illumination facilities, Induction lamp, Metal halide lamp and Natrium lamp is simulated. We study characteristics of each lamp system, which are illumination efficiencies and costs. Initial investment cost of an induction lamp is higher than two other alternatives. However, total cost of Metal halide lamp is lower than to other alternatives.

Influenza Virus Type A 감염 MDCK 세포에 있어서 Glutathione S-transferase의 활성 변동

김병렬,박남표,윤종국,전태원,이상일 대한의생명과학회 2000 Journal of biomedical laboratory sciences Vol.6 No.1

농도와 기간에 따른 바이러스 감염 및 항바이러스제인 amantadine병행 첨가 시 Maddin-Darby canine kidney (MDCK) 세포 내의 free radical 해독계 효소인 glutathione S-transferase (GST)와 lactate dehydrogenase (LDH)의 활성 변동을 상호비교 관찰하였다. 바이러스에 감염된 MDCK 세포는 감염 3일 후 1 TCID50군은 80% 이상, 10 TCID50군은 거의 대부분의 단층세포가 탈락되는 병변 효과가 나타났다. Amantadine cytotoxic dose %가 증가함에 따라 MDCK 세포 내의 GST 및 LDH 활성은 농도에 따라 유의하게 감소되었고, 감염배지 내 LDH활성은 대조군 보다 유의한 증가를 보였다. 인플루엔자 바이러스 type A접종농도와 기간에 따른 MDCK세포 내의 GST및 LDH활성은 1 및 10 TCID50감염 군에서 감염 3일 후부터 유의하게 감소되었고, 감염배지 내의 LDH활성은 10배 이상 증가되었다. 인플루엔자 바이러스 type A 100 TCID50 감염과 amantadine 병행 첨가 시 GST 및 LDH 활성은 바이러스만 감염한 군 보다 MDCK세포 내에서 대조군에 비하여 감소율이 낮았고, 감염배지 중 LDH활성 역시 증가율이 낮았다. 또한 바이러스 감염 후 amantadine 90 ㎛/ml첨가 시에 세포 내와 감염배지 중에서 가장 낮은 감소와 증가를 나타냈다. This study was performed to evaluate the activities of glutathione S-transferase (GST) and lactate dehydrogenase (LDH) in Maddin-Darby canine kidney (MDCK) cells infected with virus and/or treated with amantadine. On cell morphological findings, monolayer fractions in MDCK cells infected with virus were exfolated more than 80% in 1 TCID50 group and that in 10 TCID50 were completely exfolated after 3 days during infectious process. In proportion to the dose of amantadine, activities of GST and LDH of MDCK cells were significantly decreased and those of LDH in medium fraction were more significantly increased compared with control. According to in both dose and time of virus innoculation, activities of GST and LDH in MDCK cells were significantly decreased in 1 and 10 TCID50 infected cells after 3 days. LDH activities in infectious medium were remarkably rised at 10 fold. In case of the cell line inoculated with type A 100 TCID50 and additionally treated with amantadine, the decreasing rate to the control in activities of GST and LDH was lower than that in those in case of that infected with virus only. These results suggested that virus infection and amantadine treatment may effect the activity of the detoxicating enzyme in the target cells.