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정원줄기세포의 이종간 이식을 위한 효율적인 수여(Recipient) 생쥐 생산 기법 개발
김기중 ( Ki Jung Kim ),임한 ( Han Lim ),왕종현 ( Jong Hyun Wang ),김병각 ( Byung Gak Kim ),이용안 ( Yong An Lee ),김방진 ( Bang Jin Kim ),김용희 ( Yong Hee Kim ),홍영호 ( Yeong Ho Hong ),김근배 ( Geun Bae Kim ),류범용 ( Buom Yong 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s
Spermatogonial xenotransplantation assay provides access to the several species germline and has been used in experimental animal models to study stem cell biology and germline development. To make the xenotransplantation technology more extensively accessible, it is require development of recipient preparation protocols. Therefore, we propose an optimal method for preparation of athymic nude recipient with busulfan treatment. To investigate the optimal concentration of busulfan treatment, athymic nude mice (6~8 weeks old) were intraperitoneally injected with different concentrations of busulfan (0, 15, 25, 35 and 45 mg/kg). 6~8 weeks after busulfan treatment, testicular weight was significantly lower in 45 mg/kg treated group than the other groups. And the percentage of spermatogenic tubule was significantly reduced in 45 mg/kg of busulfan treated recipient testis. Also we compared the gene expression level of sertoli-cell-derived growth factor, glial-cell-line-derived neurotrophic factor (GDNF) as a potential measure of niche function. Relative to the other experimental groups, 45 mg/kg busulfan treated recipient testis showed higher GDNF expression. Next, to find out the appropriate time to transplantation after treated with busulfan (45 mg/kg), the rat testicular donor cells were transplanted into recipient testis at 2, 4, 6 and 8 weeks after treated with busulfan. Analysis of recipient mice revealed that transplantation of donor cells on 6 weeks after busulfan treatment was the most effective time to support high level of donor stem cell engraftment. In this study, we provide an optimum experimental condition for preparation of recipient mouse model system for xenotransplantation. Also, our data could contribute to recipient preparation in other species.
인삼의 유효 성분에 관한 생화학적 연구 ( Ⅶ ) 빵효모의 공기성 대사에 미치는 인삼 성분의 영향
김태봉,이희성,이근배,방진신 ( Tae Bong Kim,Hee Sung Lee,Keun Bai Lee,Jin Sin Bang ) 생화학분자생물학회 1975 BMB Reports Vol.8 No.2
In the aerobic metabolism of bakers` yeast using glucose as substrate, it was found that the CO₂ output was increased remarkably in the presence of 1.0-1.5% of the ginseng extracts (WGpet-alc and WGpet) : the CO₂ output increasing effect of WGpet-alc was greater than that of WGpet. Furthermore both ginseng extracts were observed to stimulate O₂ uptake of bakers` yeast and the maximum effect was shown when the concentration of WGpet-alc and WGpet were 1.0% and 1.5-3.0% respectively. In the presence of succinate as substrate, WGpet-alc showed the pronounced lowering effect on O₂ uptake and the enhancing activity of Pi-incorporation into bakers` yeast cells while WGpet had little stimulating effect on O₂ uptake and a significant inhibitory effect on the Pi-incorporation. These results suggest that WGpet-alc stimulates the oxidative phosphorylation in bakers` yeast cells and WGpet may act as an uncoupler.
WRF-UCM을 이용한 연안산업도시지역 고해상도 기상 모델링
방진희,황미경,김양호,이지호,오인보,Bang, Jin-Hee,Hwang, Mi-Kyoung,Kim, Yangho,Lee, Jiho,Oh, Inbo 한국환경과학회 2020 한국환경과학회지 Vol.29 No.1
High-resolution meteorological simulations were conducted using a Weather Research and Forecasting (WRF) model with an Urban Canopy Model (UCM) in the Ulsan Metropolitan Region (UMR) where large-scale industrial facilities are located on the coast. We improved the land cover input data for the WRF-UCM by reclassifying the default urban category into four detailed areas (low and high-density residential areas, commercial areas, and industrial areas) using subdivided data (class 3) of the Environmental and Geographical Information System (EGIS). The urban area accounted for about 12% of the total UMR and the largest proportion (47.4%) was in the industrial area. Results from the WRF-UCM simulation in a summer episode with high temperatures showed that the modeled temperatures agreed greatly with the observations. Comparison with a standard WRF simulation (WRF-BASE) indicated that the temporal and spatial variations in surface air temperature in the UMR were properly captured. Specifically, the WRF-UCM reproduced daily maximum and nighttime variations in air temperature very well, indicating that our model can improve the accuracy of temperature simulation for a summer heatwave. However, the WRF-UCM somewhat overestimated wind speed in the UMR largely due to an increased air temperature gradient between land and sea.
설치류에서 정소조직의 체외배양을 통한 정자형성과정에 관한 연구
정미선 ( Mi Seon Jung ),김방진 ( Bang Jin Kim ),이용안 ( Yong An Lee ),김기중 ( Ki Jung Kim ),김용희 ( Yong Hee Kim ),강현구 ( Hyun Gu Kang ),정상은 ( Sang Eun Jung ),김병각 ( Byung Gak Kim ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.1s
Spermatogenesis is a complex developmental process of continuous cell division involving a phase of germ cell expansion, meiotic division and cytodifferentiation that ultimately generates mature gametes. The object of this study was the development of optimal culture system in order to induce spermatogenesis in vitro and for its establishment. We collected immature testicular tissues from different developmental-stage of mice and rats which were subsequently cultured on the agarose gel with serum-free culture media. After appropriate culture period, we counted multiple layered seminiferous tubules in the cultured testis tissue to find out whether any differentiation occured following spermatogenesis. Between our experimental mouse strains ICR and C57, as a result, we observed significant increased spermatogenic tubules in case of C57 mouse strain in neonatal stage. In our optimized culture condition, we detected the lectin PNA positive spermatid resulted from spermatogenesis in cultured testis tissue after 6 weeks of incubation. We also injected the lentiviral transduced rat spermatogonial stem cells (SSCs) into recipient testis to generate the transgenic gametes. The testicular fragments were then cultured based on our optimized culture condition. After culturing 8 weeks, we observed expression of GFP in differentiated germ cells including post meiotic germ cells, which suggested the induction of donor-derived spermatogenesis. Also, in order to optimize the in vitro culture system for rat testis, we have tested the additional effect of hormones and growth factors, but could not observe the significant increased effect of hormone and growth factors treated groups than the control. In conclusion, our in vitro tissue culture methods could be applicable for production of in vitro transgenic gametes in various kinds of mammalian species including domestic animal.
김기중 ( Ki Jung Kim ),유형덕 ( Hyung Duk Yoo ),김용희 ( Yong Hee Kim ),이용안 ( Yong An Lee ),김방진 ( Bang Jin Kim ),정미선 ( Mi Seon Jung ),강현구 ( Hyun Gu Kang ),이장희 ( Jang Hee Lee ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s
The annual regrowth of deer antlers is a connatural developmental event in mammals. Therefore, studying regeneration of deer antlers could be a unique natural model of rapid and complete bone regeneration in human and other mammals. However, little is known about culture conditions and regulatory factors that stimulate growing of deer antler cells in vitro. The aim of this study was to enhance an in vitro culture efficiency of mesenchymal stem cells (MSCs) derived from deer antlers. In order to improve the culture condition, we selected minimal essential medium alpha (MEMα) as a basal medium and investigate whether serum could stimulate growing in these cells in basal medium in a dose-dependent manner. Next, to investigate the optimal temperature and O2 tension, the antler cells were cultured in different temperature and controlled O2 percentages. Through the results of number of harvested cells after 1 week, we selected MEMα, 10% fetal bovine serum (FBS), 37oC, 20% O2, and 5% CO2 tension as a basic culture conditions. Also, we could observed enhanced proliferation results by addition of the supplements [L-glutamine 2 mM, β- mercaptoethanol 100 μM, non-essential amino acid (NEAA) 0.1 mM, and HEPES 10 mM] and growth factors [basic fibroblast growth factor (bFGF) 10 ng/mL, epidermal growth factor (EGF) 20 ng/mL, insulin-like growth factor-1 (IGF-1) 10 ng/mL] and harvested antler cells strongly expressed STRO-1 and CD 90. Our results demonstrate that allow continuous proliferation of antler cells in vitro established the foundation to basic biology of antler cells and makes possible application to the regenerative medicine in a broad sence.
주요 대도시 알레르기 질환 유병률 변화와 대기오염과의 관련성 - 지역 용도를 고려한 19세 이하 주민 대상
이지호(Jiho Lee),오인보(Inbo Oh),김민호(Min-ho Kim),방진희(Jin Hee Bang),박상진(Sang Jin Park),윤석현(Seok Hyeon Yun),김양호(Yangho Kim) 한국환경보건학회 2017 한국환경보건학회지 Vol.43 No.6
Objectives: The association of air pollution levels and land-use types with changes in the prevalence of allergic diseases (allergic conjunctivitis, allergic rhinitis, asthma, and atopic dermatitis) was investigated for seven metropolitan cities in Korea Methods: Data on daily hospital visits and admissions (of those under 19 years old) for 2003-2012 were obtained from the National Health Insurance Cooperation. Meteorological data on daily mean temperature, humidity, and air pressure were obtained from the Korea Meteorological Administration. Daily mean or maximum concentration data for five pollutants (PM 10 , O 3 , NO 2 , SO 2 , and CO) as measured at air quality monitoring sites operated by the Ministry of Environment were used. We estimated excess risk and 95% confidence intervals for the increasing interquatile range (IQR) of each air pollutant using Generalized Additive Models (GAM) appropriate for time series analysis. Results: In this study, we observed a significant association between the IQR increases of air pollutants and the prevalence risk of allergic diseases (allergic conjunctivitis, allergic rhinitis, asthma, and atopic dermatitis) in all metropolitan cities after adjusting for temperature, humidity, and air pressure at sea level. Among the air pollutants, NO 2 and PM 10 were associated with the prevalence of asthma, and O 3 was associated with only allergic conjunctivitis in regression analysis. However, in GAM analysis considering land-use, O 3 and SO 2 were associated with allergic conjunctivitis, PM10, O3, NO2, and CO were associated with allergic rhinitis, and PM 10 , O 3 and NO 2 were associated with asthma in industrial area. Conclusion: This study found a significant association between air pollution and the prevalence of allergic related diseases in industrial areas. More detailed research considering mixed traffic-related air pollution (TRAP) and conducting meta-analyses combining data of the all cities is required.