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김기중 ( Ki Jung Kim ),유형덕 ( Hyung Duk Yoo ),김용희 ( Yong Hee Kim ),이용안 ( Yong An Lee ),김방진 ( Bang Jin Kim ),정미선 ( Mi Seon Jung ),강현구 ( Hyun Gu Kang ),이장희 ( Jang Hee Lee ),류범용 ( Buom Yong Ryu ) 한국조직공학과 재생의학회 2014 조직공학과 재생의학 Vol.11 No.1s
The annual regrowth of deer antlers is a connatural developmental event in mammals. Therefore, studying regeneration of deer antlers could be a unique natural model of rapid and complete bone regeneration in human and other mammals. However, little is known about culture conditions and regulatory factors that stimulate growing of deer antler cells in vitro. The aim of this study was to enhance an in vitro culture efficiency of mesenchymal stem cells (MSCs) derived from deer antlers. In order to improve the culture condition, we selected minimal essential medium alpha (MEMα) as a basal medium and investigate whether serum could stimulate growing in these cells in basal medium in a dose-dependent manner. Next, to investigate the optimal temperature and O2 tension, the antler cells were cultured in different temperature and controlled O2 percentages. Through the results of number of harvested cells after 1 week, we selected MEMα, 10% fetal bovine serum (FBS), 37oC, 20% O2, and 5% CO2 tension as a basic culture conditions. Also, we could observed enhanced proliferation results by addition of the supplements [L-glutamine 2 mM, β- mercaptoethanol 100 μM, non-essential amino acid (NEAA) 0.1 mM, and HEPES 10 mM] and growth factors [basic fibroblast growth factor (bFGF) 10 ng/mL, epidermal growth factor (EGF) 20 ng/mL, insulin-like growth factor-1 (IGF-1) 10 ng/mL] and harvested antler cells strongly expressed STRO-1 and CD 90. Our results demonstrate that allow continuous proliferation of antler cells in vitro established the foundation to basic biology of antler cells and makes possible application to the regenerative medicine in a broad sence.