한우(韓牛) 안구(眼球)의 망막신경절세포(網膜神經節細胞) 수(數)와 분포(分布)에 관(關)한 연구(硏究)

김무강,조성환,류시윤,김교준,김상근,신태균,이강이,Kim, Moo-kang,Cho, Sung-whan,Ryu, Si-yun,Kim, Kyo-joon,Kim, Song-keun,Shin, Ta-kyun,Lee, Gang-iee 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

The number and distribution of the retinal ganglion cells in the 2 years old Korean native cattle was determined from whole fiat mounted preparation stained with methylene blue and thionin. The results were summarized as follows. 1. The total number of retinal ganglion cells was estimated to be 3,085,200 in the bovine retina ranging from $2,214mm^2$ in total area. 2. Visual streak was recognized at the area 2.5mm superior to the optic disc and ganglion cell density drops off rapidly to the directions superior to and inferior to the visual streak. 3. Area centralis ($6,800cells/mm^2$) was located at the area 10mm temporally from the point of 3mm superior to the optic disc. 4. The number of ${\alpha}-type$ ganglion cells (above $15{\mu}$) was 57,000 in the bovine retina and ${\alpha}-type$ ganglion cells constituted 18.5% of the total cells. 5. The relative frequency of ${\alpha}-type$ ganglion cells was higher in the peripheral regions than in the visual streak, especially higher in the superior-temporal quadrant than in other region of the bovine retina.

영장동물폐(靈長動物肺)의 비교해부학적연구(比較解剖學的硏究) 2. 기관(氣管) 및 기관지(氣管枝)의 기하학적계측(幾何學的計測)과 폐중량(肺重量)에 관(關)한 연구(硏究)

김무강,Kim, Moo Gang,Kim, James C.S. 대한수의학회 1979 大韓獸醫學會誌 Vol.19 No.1

Anatomical measurements were made at the necoropsy and sacrifice in a group of 41 adult female Rhesus monkey to determine the 34 characteristcs found in tracheas snd bronchus. Table 1, 2, 3, 4, and figure 1 were therefore prepared as a guide in the further use for illustrating monkey lung research. Summary of this report is as follows: 1. Accurate Knowledge of these measurements may have some value in pulmonary dissection and reconstructive procedures. 2. These studies may provide a more accurate determination of the monkey lungs. 3. The right and left bronchus length of the monkey is shorter than fornd in man. 4. The left bronchus length is more longer than the rrght bronchas. 5. There were large variation among the weight of adult female Rhesus monkey.

Effects of Kainic acid on the Duck Retina

신태균,김무강,Shin, Tae-kyun,Kim, Moo-gang The Korean Society of Veterinary Science 1986 大韓獸醫學會誌 Vol.26 No.2

Kainic acid가 오리 망막에 미치는 영향을 알아보기 위하여 kainic acid $50{\mu}l$($1.0{\mu}mol$)를 오리의 안구내로 주입한 후 망막을 경시적으로 관찰하였던 바 다음과 같은 결과를 얻었다. 주사후 8시간째 망막의 내내핵층과 내망상층에서 공포화가 인정되었고 24시간째는 내핵층에서 핵농축이 현저하였으며 48시간째의 예에서는 농축된 핵이 상당수 소실되면서 망막의 두께도 다소 얇게 나타났다. 주사후 96시간과 10일째는 내핵층을 구성하는 대부분의 세포가 소실되어 망막의 두께가 상당히 얇아졌고 내망상층의 두께도 경시적으로 얇아지는 경향이었다.

Streptozotocin 에 의해 유도된 당뇨병성 통증시 Nitric Oxide 의 역할

최진정(Jin Jung Choi),전병화(Byeong Hwa Joen),윤석화(Seok Hwa Yoon),이영호(Young Ho Lee),김무강(Moo Gang Kim),김광진(Kwang Jin Kim) 대한통증학회 2001 The Korean Journal of Pain Vol.14 No.1

N/A Background: It is controversial whether the change in nitric oxide (NO) expression in the dorsal root ganglia (DRG) may be responsible for developtment and/or maintenance of painful diabetic neuropathy. The aim of this study was to clarify the role of NO in the pathogenesis of painful diabetic neuropathy. Methods: The effect of L-nitroargine methylester (L-NAME) or sodium nitroprusside (SNP) on allodynia was measured in streptozotocin (STZ)-induced diabetic rats. NO concentration was measured in the cerebrospinal fluid (CSF) and plasma of the diabetic rats. NADPH-diaphorase (NADPH-d) histochemistry was performed on the DRG and spinal cords of the STZ-induced diabetic rats. Results: L-NAME, an inhibitor of nitric oxide synthase, alleviated allodynia, while SNP, a nitric oxide donor, aggravated allodynia in diabetic rats. Plasma NO level in the diabetic rats was significantly de- creased compared with control rats. NO level in the CSF of diabetic rats did not differ from that of the control rats. NADPH-d positive cells were decreased in the DRG of diabetic rats. However, NADPH-d histochemistry in the diabetic spinal cord was not different from that of the control rats. Conclusions: Downregulation of NO expression in the diabetic rats may not be causally related to the development and/or maintenance of painful diabetic neuropathy.

鷄胎器官性長에 미치는 Mitoycin C의 影響에 關한 硏究

金武剛,金弘善,崔大卿,趙聲煥,金源式 충남대학교 의과대학 지역사회의학연구소 1981 충남의대잡지 Vol.8 No.2

Administration of Mitomycin C into various species of animals has widely been reported since last decade, however, none of them were utilized a growth formula proposed by Zimmerman and Huxley. In this paper we attempt to analyze the specific growth rate in general and organ relative growth rate using the chick embryo under the influence of Mitomycin C which is well known as an antibacterial, antiviral and antitumor by the mechanism of the alkylation. Congenital malformation of chick embryo were not considered. On the sixth day incubation, 8㎍ and 16㎍ of Mitomycin C were injected into the chorioallantoic and the egg was broken for weighing the body weight and individual organs such as brain, heart and lung on the day of 12th, 14th, 16th and 18th. The effects of the growth were analyzed by the analysis of variance method. The parameters included in growth quantity were obtained by the parameters included in growth quantity were obtained by the mathod of the last square using the measured weight, the formulas applied to analysis growth quantity and relative growth were : y=a+bt+ct^2, y=bx^2, and the results this obtained were summarized as follows : When injected with Mitomycin C, the chick embryos were observed with remarkable growth inhibition in all groups of the body, brain, lung and 14th groups of heart (p<0.005) and 12th, 16th, 18th groups of heart(p<0.05). Growth rate was increased with the development in the 8㎍ injected groups of the body, lung and 16㎍ injected groups of the lung, on the other hand, it was decreased in the both injected group of the brain and heart. The specific growth rate of all groups showed tendency of decrease in 8㎍ and 16㎍ Injected groups. The coefficient of relative growth of injected groups to control groups in the various organs showed positive allometry. The growth center of the growth gradient of all injected groups to control groups were on the eighteenth day.

Mitomycin C가 鷄胎肝臟成長에 미치는 影響에 關한 硏究

金武剛,趙聲煥,金弘善,柳時潤,李康伊 충남대학교 의과대학 지역사회의학연구소 1982 충남의대잡지 Vol.9 No.1

Mitomycin C is well known as antibacterial, antiviral and antitumor by the mechanism of alkylation. The authors intend to analyze the effects of Mitomycin C on the weight of the liver in the chick embryos. On the sixth day of incubation, 8ug and 16㎍ of Mitomycin C were injected into the chorioalantoic sac of each incubated eggs of two groups. Between the twelth day and eighteenth day of incubation a daily total of thirty injected egges of each group was broken for weighting the liver weight of chick embryos. The effects of dose and difference of growth time were analyzed by Zimmerman's growth formula and Huxley's relative growth formula and determination of significance in the levels of difference was made by analysis of variance. The results are summarized as follows: 1. Mitomycin C suppresed growth of the liver in the chick embryos and growth inhibition appeared to be much stronger in the group of 16ug dosage than that of 8ug dosage. 2. Growth rate and specific growth rate were observed to be decreased in the liver with incubation day. 3. Growth center of the growth gradient for injected groups to the control groups in the liver were on the twelfth day. 4. Relative growth of injected groups to the control group were positive allometry in both injected group, and 16㎍ injected group was more remarkable allometry than 8ug injected group.

韓國人 手掌皮膚 Meissner 小體의 量的 變化에 關한 硏究

金弘善,金武剛,趙聲煥,柳時潤 충남대학교 의과대학 지역사회의학연구소 1983 충남의대잡지 Vol.10 No.2

In order to observe the quantitative changes in Meissner's tactile corpuscles caused by aging and in different areas, 3×5 ㎟ punch excision specimens in the palm of the right and left hand from the 46 cadavers (male 35, female 11) were collected, sectioned serially, stained by the methylene blue and observed with light microscope and then analyzed a numerical changes in the palm of the right and left hand and in the site 6 and 7 in the palm of the hand. The results are summerized as follows: 1. The mean concentration of Meissner's corpuscles in the palm of the right hand was 2.92 per square millimeter and in the palm of the left hand was 5.17. There was appeared the mean concentration of Meissner's corpuscles in the palm of the hand was greater in the left than in the right. 2. In the site 6, the mean concentration of Meissner's corpuscles was 3.93 per square millimeter in the age group 21 to 50 years, 2.96 in the 51 to 60 years, 2.52 in the 61 to 83 years. There was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 3. In the site 7, the mean concentration of Meissner's corpuscles was 5.51 per square millimeter in the age group 21 to 50 years, 4.98 in the 51 to 60 years, 4.94 in the 61 to 78 years. Also, there was a decrease in the mean concentration of Meissner's corpuscles with increasing age. 4. In the site 6, the mean concentration of Meissner's corpuscles in the palm of the right hand was 3.57 per square millimeter in the age group 21 to 50 years, 2.74 in the 61 to 78 years and in the palm of the left hand was 3.07 in the 21 to 60 years, 2.24 in the 61 to 83 years. There was appeared the mean concentration of Meissner's corpuscles in the site 6 of the palm was greater in the right than in the left. 5. In tie site 7, the mean concentration of Meissner's corpuscles in the palm of the right hand was 5.12 per square millimeter in the age group 21 to 78 years and in the palm of the left hand was 5.13 in the 21 to 78 years. There was appeared the mean concentration of Meissner's corpuscles in the site 7 of the palm was a little greater in the left than in the right.

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

酪農民의 人獸共通寄生蟲 疾患에 關한 實態調査硏究

金武剛,金鍾煥,張大允 충남대학교 의과대학 지역사회의학연구소 1980 충남의대잡지 Vol.7 No.2

A Survey of zoonoses such as the Taeniasis and Cysticercosis were carried out among the dairy farmers in the some areas of Chung cheong nam Do. The results are summerized as follows : 1. Past history of Taenia infection rate was 23.1% by the 1584 Questionnaires (male 1038, female 546). Among of them the worm recovery rate was 5.1%. By the sex difference, the positive rates of Taeniasis showed that 26.3% in male and 17.0% in female and the recovery rates of the worm were 5.2% in male and 2.6% in female, and the increasing tendency of Taenia infection was found in the above 20 age group. 2. Both bovine and swine raw meat or raw visceral organs, liver and intestine, eating history from the rural inhabitants was found almost every case, and the eating place was in home, in the restaurant and in the tavern in order. 3. Incidence of human intestinal helminths was 78.6% from the 435 stool specimens examined, and the rate in Dae duck area was found higher rate than that of the Seosan, and the Taenia infection rate 6.5% in Daeduck residents and 0.9% in Seosan. 4. A total of 111 of Taeniasis cases were treated with Niclosamide, the worms, scolices and, or proglottids were expelled from 81 of the treated cases. The species identification with the 87 removed worms was performed, among of them 70 was T. saginata and 17 was T. solium. the ratio of both species was 4.7 : 1. 5. Examination of human cysticercosis from the out patient clinic of Chungnam National University Hospital as well as the local several clinics in Daejeon was observed in 17 cases. Subcutaneaus cysticercosis occurred all of the cases, and epileptic symptomes caused presumably by cerebral cysticercosis were also observed in 14 cases among of them, and Ocular cysticercosis occurred in the 3 cases out of 17 cases. 6. The incidences of Cysticercosis in the bovine was 5(0.6%) out of 803 slaughtered animals and 9(0.6%) out of 1522 slaughtered swine from the Daejeon and Dae duck public abattoir.

家兎網膜 神經節細胞의 總數와 分布에 關한 硏究

李亨周,金武剛 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

The number and distribution of the retinal ganglion cells in the rabbit was determined from whole flat mounted preparations stained with methylene blue and thionin. The results were summarized as follows. 1. The total number of retinal ganglion cells was estimated to be 903,600 in retina ranging from 641㎟ in total area. 2. The number of alpha type ganglion ceils(above 15 microns) was 118,000 in the retina of rabbit and constituted 13% of the total cells. 3. The visual streak was recognized above 2.5mm from optic disc and ganglion cell density drops off rapidly above and below the visual streak. 4. Area centralis(8,300/㎟) was recognized at the area of 10mm temporally, from the point of 2.5mm superior to the optic disc. 5. The relative frequency of the alpha type ganglion cells was higher in the superior-nasal quadrant than in other region of the retina.