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AFLP 분석을 이용한 배추과 식물의 종속간 분자유전연구
권수진(Soo-Jin Kwon),임기병(Ki-Byung Lim),임명호(Myung-Ho Lim),박지영(Jee-Young Park),김진아(Jin-A Kim),김정선(Jung Sun Kim),이수성(Soo-Seong Lee),박범석(Beom-Seok Park),진용문(Yong-Moon Jin) 한국원예학회 2007 원예과학기술지 Vol.25 No.2
식물의 유전적 다양성과 유연관계 분석은 작물개량의 기 본이다. AFLP를 이용한 핵산지문분석법은 식물 뿐 아니라 다른 생물체의 유전자지도 작성에서부터 유전적 다양성 조 사에까지 널리 응용되고 있는 강력한 기술 중 하나이다. 배추속 작물 중 2배체인 기본종과 기본종들간 교잡에 의해 생성된 이질4배체, 종간 혹은 속간 잡종 그리고 이들의 양친을 대상으로 AFLP 양상을 관찰하였다. AFLP 반응에는 두개의 프라이머 쌍이 사용되었고 7개의 EcoRI(+2)/MseI(+3) 프라이머 조합이 유전적 유연관계의 측정에 이용되었다. 9개 accession 간의 유전적 유사도는 Nei의 유전적 거리 산출식에 의해서, 이들간 군집분석은 NTSYS-pc 프로그램의 UPGMA를 이용하였다. FISH를 통해 배추(Brassica)와 무(Raphanus)의 속간 잡종 1세대(배무채)의 염색체를 관찰한 결과 배추와 무의 염색체 수가 일치한 19쌍의 염색체(2n=4x=38)가 관찰되었으며 배추 염색체내에 무염색체 일부가 치환되었음을 확인할 수 있었다. Genetic diversity and relationship in the related plant taxa is a prerequisite for crop improvement. Amplified fragment length polymorphism (AFLP) fingerprinting is one of the powerful and widely used tools, which has found wide spread application in numerous studies ranging from genetic mapping to diversity surveys in plants and other organisms. Genome relationship among diploid and allotetraploid Brassica taxa, and between interspecific hybrid or intergeneric hybrid and their parental species were investigated using AFLP display. The two primer pairs for AFLP reactions and seven EcoRI (+2) and M5eI (+3) primer combinations were used in the measurement of genetic relationships. Genetic similarities among nine accessions were estimated by Nei’s distance equation, and cluster analysis was performed using the unweighed pair group method (UPGMA) in the computer program NTSYS-pc. One intergeneric hybrid between Brassica and Raphanus showed 2n=4x=38 chromosomes, of which Raphanus chromosome segment were translocated with B. rapa chromosome.
대학생의 식행동, 흡연, 음주실태와 주관적 건강상태와의 관련성
권수진 ( Soo Jin Kwon ),강지혜 ( Ji Hea Kang ),김나정 ( Na Jung Kim ),김려진 ( Rye Jin Kim ),김선영 ( Suhn Young Kim ),김시일 ( Si Il Kim ),한경순 ( Gyeong Soon Han ) 한국치위생과학회 2012 치위생과학회지 Vol.12 No.2
This study aims at investigate dietary behaviors, smoking and drinking status of university students depending on their gender and residential patterns, and analyzing relationships with their subjective health status. The subjects of this study was 538 university students in Metropolitan areas from May 10-31, 2010. Data were analyzed with chi-square test, fisher`s exact test, t-test and stepwise multiple regression SPSS 12.0. Male students higher frequency on the intake of eggs, meats and carbonated drinks than female students. The group residing at one`s own house showed higher frequency on the intake of beans, fruits and milk than the self-boarding group or other groups(p<0.05), while preferring sweet taste the most. Male students were significantly higher than female students in the amount of smoking and drinking(p<0.05). They had positive recognition on their health conditions as they have higher frequency on the intake of potatoes, seaweeds, green tea and milk with less frequency on the intake of eggs, fast foods and drinking amount. Accordingly, it was considered necessary to have continuous education on nutrition and social supports to prevent their dietary behaviors from leaning on convenience or preference.
경남지역 청소년들의 치과진료형태가 치과공포감에 미치는 영향
권수진(Soo-Jin Kwon),최유진(Yu-Jin Choi) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.2
본 연구는 청소년들의 치과진료형태에 따른 치과공포감의 영향관계에 대해 규명하여 청소년의 치과진료 시 불안, 공포감을 완화시켜줄 수 있는 방법을 모색하고자 하였다. 조사대상은 경남지역에 재학 중인 청소년을 대상으로 2009년 12월 2일부터 9일까지 8일간 설문조사를 실시하여 420부를 분석에 사용하였다. 연구결과 상관분석을 통한 치과진료 경험과 공포감의 관계에서 각 구성요소 간 유의한 상관성을 보였고, 회귀분석 결과 전체적인 공포감에는 치과보철 진료경험이, 치과진료 회피 공포감에는 치과보존 진료경험이, 생리적 반응요인 공포감에는 구강악안면 진료경험이, 치료자극 반응요인 공포감에는 치과보철 진료경험이 유의한 영향을 미치는 것으로 나타났다. 이에 향후 치과 의료기관은 청소년들이 치과진료에 대한 막연한 공포나 두려움을 없애고, 정기적인 치과방문을 통한 예방진료 등의 강화로 구강건강증진을 도모하고 건강한 삶을 영위할 수 있도록 진료형태별?대상별 치과 공포감을 없앨 수 있는 매뉴얼 개발 등의 노력이 필요할 것으로 생각된다 The purpose of this study was to examine the influential relationship between the type of dental treatment and the dental fear of teens in a bid to seek ways of relieving adolescents of dental anxiety and fear. The subjects were teenaged students in South Gyeongsang Province. After a survey was conducted for eight days from December 2 to 9, 2009, the answer sheets from 420 students were analyzed. The findings of the study were as follows: When a correlation analysis was employed to look for connections between dental treatment experiences and dental fear, the components of dental treatment experiences had a significant correlation to those of dental fear. As a result of making a regression analysis, overall dental fear was under the significant influence of prosthodontic treatment experiences, and avoidance of dentistry was significantly affected by conservative treatment experiences. Oral and maxillofacial treatment experiences had a significant impact on physiologic response, and dental stimulus was significantly affected by prosthodontic treatment experiences. Therefore dental institutions should direct their efforts into the development of manuals geared toward different types of treatment and different patients in order for adolescents to receive dental treatment without any anxiety or fear, to get a regular dental checkup, to receive more preventive treatment, to promote their oral health and ultimately to lead a healthy life.