벼에서 RAPDs 분석에 의한 양친의 유전적 유사성과 잡종강세의 상관

안상낙,김윤선,Ahn, Sang-Nag,Kim, Yun-Sun Institute of Agricultural Science 2001 Korean Journal of Agricultural Science Vol.28 No.1

RAPDs 분석에 의한 양친의 유전적 유사성 정도가 F1 잡종강세 예측에 효율적으로 이용될 수 있는지 알아보고자 통일형 6 품종(밀양42, IR747, 백운찰벼, 삼강벼, 수원307, 이리356) 간 반이면 교배 F1 15조합을 작성하였다. 양친과 F1을 공시하여 조사한 수량 등 8개의 특성과 RAPDs 분석을 통해 구한 양친의 유전적거리와의 관계를 조사하였다. 120개의 Operon 프라이머를 6개 품종의 DNA 증폭에 이용한 결과 3367개의 밴드가 발생되었으며 이중 168개가 6개 품종 중 1개 이상의 품종에서 변이를 보였다. 6품종간 유전적 거리는 최소 0.157(백운찰벼와 수원 307호 간), 최대 0.383(삼강벼와 이리356호 간)의 분포를 보였다. 잡종강세의 정도는 정조수량(129%), 수당립수(125%), 수장(109%) 등이 높은 잡종강세를 보였고, 유전적거리는 수당립수와 통계적으로 유의한 부의 상관을 보였지만, 정조수량, 천립중 등과는 유의한 상관을 보이지 않았다. In this study we evaluated genetic divergence among six Tongil-type rice varieties and assessed the relationship between genetic distance and hybrid performance in all possible non-reciprocal crosses between them. The 15 F1 hybrids along with the six parents were evaluated for eight traits of agronomic importance including yield in a replicated field trial. The six parents were examined for DNA polymorphism using 120 random decamer oligonucleotide primers. A total of 168 polymorphic variants were generated and based on the polymorphism data, genetic distances (GDs) ranged from 0.157 to 0.383. Heterosis was observed in hybrids for most of the traits, and yield exhibited the highest heterosis among the eight traits examined. The correlation values of GDs with F1 performance were not significant. Also the correlations of GDs with midparent and better-parent heterosis were not significant except for spikelets per panicle. Our results have indicated that GDs based on the RAPDs markers may not be useful for predicting heterotic combinations in Tongil-type rice and are supportive of the idea that the level of correlations between hybrid performance and genetic divergence is dependent on the germlplasm employed.

Relationship Between Heterosis and Genetic Divergence in Tongil-type Rice(Oryza sativa L.)

Sang-Nag Ahn(안상낙),Yun-Sun Kim(김윤선) 충남대학교 농업과학연구소 2001 농업과학연구 Vol.28 No.1

Identification of AFLP Marker Linked to Clubroot-resistant Gene in Chinese Cabbage Using Bulked Segregant Analysis

Piao Zhong Yun(박종운),Sang Nag Ahn(안상낙),Chang Soon Jang(장창순),Yong Pyo Lim(임용표) 한국원예학회 2000 원예과학기술지 Vol.18 No.2

우리나라 재래벼의 유전적 다양성

권수진(Soo Jin Kwon),안상낙(Sang Nag Ahn),서정필(Jung Pil Suh),홍하철(Ha Cheol Hong),김연규(Yeon Kyu Kim),황흥구(Hung Goo Hwang),문헌팔(Huhn Pal Moon),최해춘(Hae Chune Choi) 한국육종학회 2000 한국육종학회지 Vol.32 No.2

Gas chromatography를 이용한 향미 유전자원의 2-acetyl-1-pyrroline 정량분석

김정순(Jeong-Soon Kim),박원성(One-Sung Park),안상낙(Sang-Nag Ahn),이정로(Jung-Ro Lee),곽재균(Jae-Gyun Gwag),김태산(Tae-San Kim),이석영(Sok-Young Lee) 한국식품과학회 2008 한국식품과학회지 Vol.40 No.5

본 실험은 국내 육성향미자원과 유전적 배경이 서로 다른 도입향미 유전자원의 2AP 함량을 비교분석하여, 2AP 함량이 높고 농업적 형질이 우수한 자원을 선발하여 우수한 향미자원의 육성에 필요한 기초 자료를 제공하고자 실시하였다. 3종류의 유기용매에 대한 추출효율은 ethanol>acetonitle>methanol 순으로 확인되었으며, ethnanol의 경우 30-90℃의 온도범위에서 3가지 유기용매 중 2AP 추출효과가 가장 뛰어났고, 특히 90℃에서 30분간 추출하였을 때 2AP 추출함량이 가장 높았고 추출 시간이 경과할수록 2AP의 함량이 낮았다. 현미의 분쇄정도에 따른 2AP 추출함량은 5초간 분쇄하였을 경우 가장 높았으며 분쇄 시간이 길어질수록 낮아졌다. 선발한 30품종 중 국내 향미자원과 자포니카형 도입자원의 평균 출수일은 각각 130.3일과 131.8일로 비슷하였으며 인디카형 도입자원은 약 137.1일로 가장 늦었다. 평균 1,000립중은 인디카형 도입자원이 19.9 g으로 가장 낮았으며, 국내 향미자원이 21.65 g이었고 자포니카형 도입자원은 25.5 g으로 높았다. 선발자원의 향에 대한 관능검사는 국내 향미자원 5품종 중향남벼 2호, 아랑향찰벼가 중간 정도의 향을 띄었고 인디카형 도입자원 19품종 중에서 11품종(57.9%)과 자포니카형 도입자원 6품종 중 2품종(33.3%)이 중간 이상의 향을 나타났다. 선발한 30품종의 평균 2AP 함량은 국내 육성 향미자원이 도입 향미자원에 비해 낮았으며 도입자원에서는 인디카형이 자포니카형보다 높은 2AP 함량을 나타내었다. 국내 육성 향미자원은 아랑향찰벼 외 4품종과 도입 향미자원 중 인디카형은 Basmati6313 외 18품종 및 자포니카형은 자향나 861 외 5품종을 선발하였다. This study was conducted to optimize the analysis condition and quantify the 2-acetyl-1-pyrroline (2AP) in the brown aroma rice. Extraction effect of the solvent for 2AP was the order of ethanol>acetonitrile>methanol in the range from 30 to 90℃. In the extraction time of 15, 30, 60, and 90 min, the 30 min had the highest 2AP concentration, and it was decreased according to lapse of time. At grinding time, 5 sec resulted in highest 2AP concentration. It was recommended that five sec grinding time, using ethanol, at 90℃ for 30 min was the optimization conditions to quantify the 2AP. Hyangmibyeo2ho and Aranghyangchalbyeo were mild aroma. In the foreign aroma rice, 11 of 19 accessions of Indica types and 2 of 6 accessions of Japonica types were more than mild aroma. Finally, 30 accessions of aroma rice were selected based on their 2AP concentration and agronomic traits.

일품벼×모로베레칸 조합 여교잡 BC3F2 계통 특성 평가

구홍광(Hong Guang Ju),윤동범(Dong Beom Yoon),사소파(Xiao Bo Xie),안상낙(Sang Nag Ahn),정오영(Oh Young Jeong),정응기(Eung Gee Jeong),최경자(Kyung Ja Choi) 한국육종학회 2004 한국육종학회지 Vol.36 No.5

During the progress of introgressive backcrossing between Ilpumbyeo as a recurrent parent and Moroberekan as a donor to develop promising lines resistant to rice blast, 279 BC₃F₁ 수식 이미지plants which were phenotypically similar to Ilpu

벼 일품벼/모로베레칸 조합의 이입계통을 이용한 내건성 유전자 탐지

강주원(Ju-Won Kang),구홍광(Hong-Guang Ju),양바오로(Paul Yang),안상낙(Sang-Nag Ahn) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2

This study was conducted to map quantitative trait loci (QTLs) related to drought stress tolerance. An introgression line population derived from a cross, “Ilpum” / “Moroberekan” was used in this study. F₁ plants were backcrossed three times to Ilpum to produce BC₃F₁ plants. These plants were advanced by selfing for four generation and a total of 117 BC₃F? introgression lines were developed. These lines were evaluated for percent seed set and spikelets per panicle under the control (field) and drought condition. To identify QTLs related to drought tolerance, 134 SSR markers showing polymorph isms between the parents were genotyped for the 117 BC₃F? lines. A total of 6 QTLs associated with drought stress were detected on chromosomes 1, 3, 4, 7 and 10. These include two QTLs for phenotypic acceptability, two QTLs for percent seed set (R² = 19.0 - 20.9%), and two QTLs for spikelets per panicle (R² = 22.3 - 23.10%). The Moroberekan alleles at three loci contributed the positive effect for drought tolerance. The SSR markers linked to drought stress tolerance can not only facilitate the selection of valuable genes from Moroberekan, but also allow identification of lines with drought tolerance.

벼 종간잡종 유래 근동질 유전자계통 이용 종자중 관여 유전자 분석

강주원(Ju-Won Kang),양바오로(Paul Yang),윤여태(Yeo-Tae Yun),안상낙(Sang-Nag Ahn) 한국육종학회 2011 한국육종학회지 Vol.43 No.4

1. 선행 연구에서 염색체 3번의 RM60~RM231 부근에서 종자중, 수당립수에 관여하는 QTL이 탐지되었고, 이를 확인하기 위하여 이 지역에 크기와 위치가 다른 O. glaberrima 단편이 이입된 5 계통을 선발하여 표현형을 조사하였다. 실험 결과 계통별로 출수기, 임실률, 수당립수, 종자중을 제외한 형태적 특성들이 밀양23호와 비슷한 양상을 보였다. 이는 대부분의 염색체 지역이 밀양23호로 회복되었기 때문이라고 보여진다. 2. 유전자의 위치를 자세히 알기 위해 RM60과 RM22 부위에 위치하는 SSR 마커를 이용하여 5개 계통의 유전자형을 검정하였다. 종자중과 수당립수에서 차이를 보이는 4계통, IL3, IL26, IL25와 IL51을 비교한 결과 종자중과 수당 립수를 조절하는 유전자는 RM60-RM523 사이의 재조환 지점과 단완 끝 부분 사이에 위치하는 것으로 판단되며 그 거리는 약 1.2-Mb이다. 3. 본 연구에서 탐지된 연관된 종자중과 수당립수 QTL은 재 배벼의 수량성 증진에 효과적으로 이용될 수 있을 것으로 판단된다. In previous studies, we reported QTLs for grain weight (GW), qGW3 and for spikelets per panicle (SPP), qSPP3 linked to RM60 on chromosome 3 using advanced backcross lines derived from a cross between Oryza sativa ssp. Indica cv. Milyang 23 and O. glaberrima. The O. glaberrima alleles at this locus increased GW and spikelets per panicle in the Milyang 23 background. To further confirm and narrow down the position of the QTLs on chromosome 3, substitution mapping was performed using five lines containing the target O. glaberrima segment on chromosome 3. The size and position of the O. glaberrima segment on chromosome 3 were different in each line. These lines possessed 3-10 non-target O. glaberrima introgressions in the Milyang 23 background. These five lines were evaluated for seven agronomic traits including 1,000 grain weight and spikelets per panicle and also genotyped with seven SSR markers. Four lines were informative in delimiting the position of QTLs, qGW3 and qSPP3. Two lines with the O. glaberrima segment flanked by SSR markers, RM60 and RM523 displayed significantly higher values than Milyang 23 in GW and SPP whereas two lines without that O. glaberrima segment displayed no difference in GW and SPP compared to Milyang 23. The result indicates that two QTL, qGW3 and qSPP3 are located in the interval between RM60 and RM523 which are 1.2-Mb apart. Introgression lines having QTLs, qGW3 and qSPP3 would be useful materials not only to indentify the relationship between these two yield QTLs, but also to develop high yielding variety via marker-aided selection technology.

Microsatellite를 이용한 자포니카 벼의 다양성 분석

나소(Luo Xiao),상세티(Sangshetty Balkunde),양바오로(Paul Yang),이현숙(Hyun-Sook Lee),안상낙(Sang-Nag Ahn) 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.1

The study was conducted to evaluate the genetic similarity among commercial japonica rice varieties in Korea and China and to develop markers to differentiate between japonica cultivars developed in Korea and China. The genetic similarity and cluster of 38 accessions were analyzed using 47 SSR(simple sequence repeat) markers. The number of alleles by 47 SSR markers ranged from 2 to 9 with an average of 3.6. A total of 169 alleles were detected among these tested rice varieties. The PIC value varied from 0.05 to 0.79 with an average of 0.44. The Chinese japonica cultivars could be differentiated from the japonica cultivars in Korea by combining 2 SSR markers, RM223 and RM266. Cluster analysis showed that 38 tested varieties could be distinguished into japonica and indica based on the genetic distance.

밀양23호/Oryza glaberrima 조합 이입계통의 내건성 특성 평가

강주원(Ju-Won Kang),김동민(Dong-Min Kim),상세티(Sangshetty),이현숙(Hyun-Sook Lee),안상낙(Sang-Nag Ahn) 한국육종학회 2015 한국육종학회지 Vol.47 No.1

We generated 55 introgression lines via backcrosses using Milyang23 as the recurrent parent and Oryza glaberrima as the donor. 141 SSR markers were used to genotype 55 introgression lines. The 55 introgression lines with Milyang23 were evaluated for physiological traits; fresh shoot weight (FSW), fresh root weight (FRW) and dry shoot weight (DSW) under the control and 30% PEG-treated condition. Three lines showing significant difference with Milyang23 were selected for further analysis. Three lines had four, four and two O. glaberrima homozygous segments, respectively. IL9 performed better than Milyang23 in all traits measured in the 30% PEG-treated condition. IL9 possessed four O. glaberrima introgressions on chromosomes 1, 2, 6 and 7. IL12 performed better than Milyang23 in FSW and FRW. IL55 contains two O. glaberrima introgressions on chromosomes 2 and 6. Three lines shared the O. glaberrima segment delimited by markers RM133-RM225 at chromosomes 6. This region corresponds to the QTL region for drought tolerance reported by other previous studies. Although IL9 and IL12 showed improved drought tolerance at the seedling and vegetative stage, they performed poor under the drought stress at the reproductive stage implying that the level of drought tolerance differs according to the growth stage in rice. IL55 had significantly higher no. of the total grain than Milyang 23. This result seems to indicate that IL55 will be a good resource for drought tolerance breeding. The population would be useful in developing drought tolerant lines in the breeding program.