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- 저자 : 권무식(Kwon, Moo-Sik) (검색결과 59 건)
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한국인 혈액응고 제 9인자의 제한효소 절편 길이 다형성 분석
권무식(Moo Sik Kwon),이정민(Jeong Min Lee),전봉균(Bong Kyun Jeon),오성관(Sung Gwan Oh),류종석(Chong Suk Ryou),오보훈(Bo Hoon Oh) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.3
N/A Objective : The purpose of this study was to investigate the methods for analysis of restriction fragment length polymorphisms of hemophilia B (coagulation factor Ⅸ) gene in Korean population. Methods : Genomic DNAs were extracted from 40 Korean females. In order to amplify genomic DNAs at the region of the polymorphic sites, two sets of primers (Hha I and Dde I) were synthesized. The primers were named as FIX1, FIX2 for Hha I, and Dde I 59, Dde I 39 for Dde I, respectively. Hha I primers annealed 3'-flanking region of the Factor Ⅸ gene and amplified 230 bp long fragment. The PCR fragment (230 bp) treated with Hha I endonuclease produced two fragments (150 bp and 80 bp), when the polymorphic site existed. Dde I primers annealed the region of the first intron of Factor Ⅸ gene and amplified 319 bp long fragments. People cases with Dde I polymorphic site are supposed to produce 369 bp long fragment. Results : It has been found that seven (14 X chromosomes) out of forty individuals showed Hha I polymorphism. However, none of the experimental People cases showed the Dde I polymorphism. Conclusion : By the analysis of 80 chromosomes, the PICs calculated from allele frequency of Hha I-RFLP (0.175/0.825) and that of Dde I-RFLP (0.0/1.0) were 0.289=[1-(0.1752+0.8252)] and 0=[1-(02+12)], respectively. From these results, it can be postulated that Hha I and Dde I polymorphisms of the Factor Ⅸ gene in Korean exhibited different patterns from those of Caucasian.
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권무식,Kwon, Mu-Sik 한국통신학회 2007 韓國通信學會論文誌 Vol.32 No.2C
영상에서 사람의 머리위치를 찾는 문제에 있어서 어깨선 정보를 이용하는 것은 아주 유용하다. 영상에서 머리 외곽선과 어깨선의 형태는 일정한 변형을 유지하면서 같이 움직이므로 이를 ASM(Active Shape Model) 기법을 사용해서 통계적으로 모델링 할 수 있다. 그러나 ASM 모델은 국부적인 에지나 그래디언트에 의존하므로 배경 에지나 클러터 성분에 민감하다. 한편 AAM(Active Appearance Model) 모델은 텍스쳐 등을 이용하지만, 사람의 피부색, 머리색깔, 옷 색깔 등의 차이로 인해서 통계적인 학습방법을 쓰기가 어렵고, 전체 비디오에서 외모(Appearance)가 시간적으로 변한다. 따라서, 본 논문에서는 외모(Apperance) 모델을 변화에 따라 바꾸는 대신, 영상의 각 화소를 머리, 어깨, 배경으로 구분하는 분별적 외모 모델(discriminative appearance)를 사용한다. 실험을 통해서 제안된 방법이 기존의 기법에 비해서 포즈변화와 가려짐, 조명의 변화 등에 강인함을 보여준다. 또한 제안된 기법은 실시간으로 작동하는 장점 또한 가진다. Associating the shoulder line with head location of the human body is useful in verifying, localizing and tracking persons in an image. Since the head line and the shoulder line, what we call ${\Omega}$-shape, move together in a consistent way within a limited range of deformation, we can build a statistical shape model using Active Shape Model (ASM). However, when the conventional ASM is applied to ${\Omega}$-shape fitting, it is very sensitive to background edges and clutter because it relies only on the local edge or gradient. Even though appearance is a good alternative feature for matching the target object to image, it is difficult to learn the appearance of the ${\Omega}$-shape because of the significant difference between people's skin, hair and clothes, and because appearance does not remain the same throughout the entire video. Therefore, instead of teaming appearance or updating appearance as it changes, we model the discriminative appearance where each pixel is classified into head, torso and background classes, and update the classifier to obtain the appropriate discriminative appearance in the current frame. Accordingly, we make use of two features in fitting ${\Omega}$-shape, edge gradient which is used for localization, and discriminative appearance which contributes to stability of the tracker. The simulation results show that the proposed method is very robust to pose change, occlusion, and illumination change in tracking the head and shoulder line of people. Another advantage is that the proposed method operates in real time.
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Pasteurella multocida의 외막 단백질 H에 의해 유도되는 방어적 항체와 면역
권무식,김영봉,이정민,Kwon, Moo-Sik,Kim, Young-Bong,Lee, Jeong-Min 한국미생물학회 2007 미생물학회지 Vol.43 No.1
Pasteurella multocida는 돼지에서 위축성 비염, 폐렴을 비롯한 다양한 호흡기 질환을 일으키는 병원균이다. 본 연구에서는 돼지 위축성 비염에 대한 효과적인 순수정제 백신을 개발하고자 하는 기초 연구로서 P. multocida의 외막 단백질 H에 의해 유도되는 방어적 항체와 면역을 확인하였다. P. multocida의 외막 단백질을 포함하는 분획은 호흡기 질병 혼합 백신에 대한 항혈청과 불활화된 사균 세포에 대한 항혈청 모두에서 면역학적으로 검출 가능하였다. 선행 연구에서 분리한 외막 단백질 H 유전자는 재조합 발현 백터 제작에 이용되어 대장균으로부터 재조합 외막단백질 H를 정제하였다. 실험 동물 면역과 항혈청의 교차반응, ELISA를 통한 항체 역가의 측정 및 공격접종을 통하여, 재조합 외막 단백질 H는 높은항원성을 가지며, 지속적인 체액성 면역을 유도하는 것을 확인하였다. 외막 단백질 H는 순수정제 항원으로서 P. multocida에 의한 호흡기 질환에 대한 효과적인 방어를 유도할 수 있는 단위 백신 후보 단백질로 여겨진다. Pasteurella multocida is one of the important animal pathogen causing widespread infections in various domestic animals. In swine, it causes severe respiratory diseases such as atrophic rhinitis and pneumonic pasteurellosis. To develop the efficient subunit vaccine against swine atrophic rhinitis, we investigated protective antibodies and humoral immunity of outer membrane protein H (OmpH) which is one of the major outer membrane proteins in P. multocida. Outer membrane fraction of P. multocida was immunologically detectable using antisera from both mice groups vaccinated by formalin-killed whole cells and by commercial vaccine. The expression vector for production of recombinant OmpH was constructed and the recombinant OmpH was expressed and purified from E. coli. Recombinant OmpH showed high antigenic and immunogenic properties in mice vaccination and ELISA with antisera.
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파스튜렐라(A : 3) 균주의 재조합 외막단백질 H에 의한 가금 콜레라 감염 생쥐의 면역성 검정
김영환,양주성,권무식,Kim, Younghwan,Yang, Joo-Sung,Kwon, Moosik 대한수의학회 2006 大韓獸醫學會誌 Vol.46 No.2
Pasteurella multocida is a terrible veterinary pathogen that causes widespread infections in husbandry. To induce homologous and/or heterologous immunity against the infections, outer membrane protein Hs (OmpH) in the envelope of different strains of P. multocida are thought to be attractive vaccine candidates. Previously we cloned and characterized a gene for OmpH from pathogenic P. multocida (A : 3) (In Press, Korean J. Microbiol. Biotechnol. 2005, 33, December). The gene is composed of 1,047 nucleotides (nt) coding 348 amino acids (aa) with signal peptide of 20 aa. The truncated ompH, a gene without nt coding for the signal peptide, was generated using pRSET A to name "pRSET A/OmpH-F2". This truncated ompH was well expressed in Escherichia coli BL21 (DE3). Truncated OmpH was purified for induction of immunity against live pathogen of fowl cholera (P. multocida A : 3) in mice. Some $50{\mu}g$ of the purified polypeptide was intraperitoneally injected into mice two times with 10 day interval. Lethal dose ($25{\mu}l$) of live P. multocida A : 3 was determined by directly injecting the pathogen into wild mice (n = 25). To demonstrate the vaccine candidate of the truncated OmpH, the live pathogen ($25{\mu}l$) was challenged with the OmpH-immunized mouse group as well as positive & negative controls (n = 80). The results show that the truncated OmpH can be used for an effective vaccine production to prevent fowl cholera caused by pathogenic P. multocida (A : 3).
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재래종 쌀보리의 형태적 특성 및 RAPD에 의한 유연 관계 분석
조원경,이정민,권무식,정태영,Cho, Won-Kyong,Lee, Jeong-Min,Kwon, Moo-Sik,Chung, Tae-Young 한국식물생명공학회 2002 식물생명공학회지 Vol.29 No.4
재래종 쌀보리 들은 추파형이며 내한성이 약하고 장간이면서 밀수, 장망으로 출수가 늦은 품종이 많았다. 그러나 파성, 내한성, 출수기, 간장, 수장의 변이가 컸으며, 내한성이 강한 품종, uzu 유전자, 찰성, 자색종피 등 다양한 유용 돌연변이를 보였다. 재래종들의 유연관계를 형태적 특성과 RAPD분석 결과, 112품종 모두 다른 품종으로 밝혀졌으며, 재래종 쌀보리와 겉보리의 유전적 특성을 비교한 결과, 일양성이 인정되어 쌀보리 품종들이 겉보리 재래종으로부터 돌연변이 되었던 것으로 추정할 수 있었다. Barley varieties collected from 1940 to 1951 allover the Korean peninsula by Dr. Takahashi Ryuhei were reintroduced from the Research Institute for Bioresouces in Okayama University, Japan, and the evaluation of morphological characteristics and RAPD analysis were performed. Totally, 493 varieties of Korean barley landraces were planted in the green house, from which seeds of 491 varieties were harvested and conserved in the seedbank of the Rural Development Administration. Majority of the naked barley varieties showed dense spikes with long awn, late heading, winter habits, and long plant height. However, variants having various phenotypes such as short awn, blue aleurone color, brachytic type and waxyness were also identified. Plant height, spike length, and cold-tolerance in the varieties were also highly variable among them. Homogeneity tests on the variation of growth habits, spike density, anthocyanin pigmentation on the seed coat, and hairiness on leaf sheath between naked and covered barley showed that the variations of naked barley were similar to those of covered barley. It maybe indicate that the most of naked barley landraces were mutated from the covered barley landraces. Korean landraces of naked barley were broadly divided into 4 groups by the dendrogram produced by morphological characteristics; however, the identities of the group were rather indistinct. Many varieties, belonged to the same group, were showed different band patterns in RAPD analysis using 5 different primer sets. These results indicate that the 112 varieties of naked barley landraces were different genotypes.
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