C3H 생쥐에서 자외선 B의 집중조사와 분할조사가 표피내 랑게르한스 세포의 회복에 미치는 영향

구상완,한승경,박윤기,조무연 ( Sang Wahn Koo,Seung Kyung Hann,Yoon Kee Park,Moo Yon Cho ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.4

N/A The Effect on the Recovery of Epidermal Langerhans Cells in C3H Mice after Single and Fractionated Exposure of Ultraviolet B Irradiation Sang-Wahn Koo, M.D., Seung Kyung Hann, M.D., Yoon-Kee Park, M.D., Moo Yon Cho, M.D. Department of Dermatology, Yonsei University College of Medicine Seoul, Korea The study was undertaken to see the recovery in epidermal Langerhans cells in relation to time after UVB irradiation through different amounts and ways of exposure in C3H mice. We irradiated the ears of C3H mice with UVB 200mJ/㎤ and 400mJ/㎤ in a single dose at one time and 5 fractionated doses for 5 days and 10 fractionated doses for 10 days and performed biopsies on the ears of the control and experimental groups after, 2, 7, 14, 21 days of irradiation and stained them by the immunoperoxidase method. The results are summarized as follows. 1. A more significant decrease in the number of Ia-positive epidermal Langerhans cells was observed in single-dose-exposed group than fractionated-dose-exposed groups on the second day of irradiation. 2. On the seventh day of exposure, the number of the Ia-positive epidermal cells in single-dose-exposed group returned to normal, but in fractionated-dose-exposed groups the number of Langerhans cells decreased most remarkably. 3. In fractionated-dose-exposed groups, the number of Langerhans cells returned to normal on the 14th or 21st day of expposure. (Kor J Dermatol 29(4) : 459-465, 1991)

자외선 조사에 따른 인체 피부의 노화현상 및 p53 표현성

구상완 ( Sang Wahn Koo ),박대현 ( Dae Hyun Ban ),김영근 ( Young Keun Kim ),한승경 ( Seung Kyung Hann ),박윤기 ( Yoon Kee Park ) 대한피부과학회 1998 대한피부과학회지 Vol.36 No.2

Background: Long-term phototherapy can induce the changes of photoaging and it is reported that there is an increased chance of cutaneous squamous cell carcinomas in patients exposed to large amounts of UV radiation. Objective : The main objective was to investigate the degree of photoaging and the presence of p53 mutations in normal skin in patients undergoing long-term phototherapy. Method: We performed hematoxylin-eosin and special stains, p53 and p21 immunohistochemical stains and polymerase chain reaction - single-strand conformation polymorphism (PCR-SSCP) on the normal skin of patients subject to long-term UV therapy. Result: 1. The typical features of photoaging were not observed in patients undergoing long-term UV therapy. 2. In p53 immunohistochemical staining performed at 1 week after cessation of long-term PUVA treatment, the patient group with a culmulated UV dosage of more than 1,000J/cm2 demonstrated an increased number of p53 positive epidermal cells compared to exposed as well as unexposed normal skins. 3. The patterns of p21 immunohistochemical staining performed at 1 week after cessation of long-term PUVA and UVB treatments were similar to that of p53 immunohistochemical staining performed at 1 week after cessation of phototherapy. 4. In p53 immunohistochemical staining performed at 4 months after cessation of UV treatment, the number of p53 positive epidermal cells decreased significantly compared to that of p53 positive epidermal cells found at 1 week after cessation of UV treatment. 5. The mutation of p53 genes was not found in PCR-SSCP analysis of biopsied skins done at 1 week after cessation of long-term PUVA and UVB treatment. Conclusion : Long-term phototherapy did not induce the typical changes of photoaging and p53 overexpression in the epidermis of UV treated skin was a reactive process. Therefore, UV therapy can be a relatively safe treatment modality, although a closer observation for cutaneous malignancy is warrented in the patients whose cumulated UV dosage is much higher than 1,000J/cm2. (Korean J Dermatol 1998;36(2): 224-235)

초선택 전색으로 치료한 안면 동정맥 기형

구상완,김동건,김동익 ( Sang Wahn Koo,Dong Kun Kim,Dong Ik Kim ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.5

Superselective embolization, which enables vital vessels to be protected, while embolizing feeding vessels to the nidus only, has been popularized by Serbinenko in 1971 and there after it has beem used in the treatment of arteriovenous malformation(AVM) mainly for two purposes;first, as a preoperative adjunct to decrease vascularity prior to excision and second, as the principal mode of therapy in unresectable AVM`s. A 23-year-old male was presented with a purple-colored nodule o? 4㎝ in diameter on the left cheek. Angiography revealed an AVM supplied with the oranches of the facial, transverse facial and maxillary arteries. Polyvinyl alcohol, a nonabsorbable permanent solid embolic material, was used to occlude the branches to the nidus, and after the procedure the angiogram demonstrated greatly reduced blood supply to the nidus of the tumor. After a month of the embolization, the tumor decreased considerably in size with the improvement of color.

비전형적인 임상양상을 보인 안면 백선증 2예

허원(Won Hur),구상완(Sang Wahn Koo),한승경(Seung Kyung Hann),방동식(Dong Sik Bang) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.3

Tinea faeiei is an unusual fungal infection which often is not recognized by clinieians. Because of light sensitivity, the frequent absence of typical clinical manifestation, and secondary changes due to steroid treatment, this condition is frequently misdiagnosed with other dermatoses. We described two patients who showed atypical facial lesions which were histologically diagnosed as tinea faciei. The case I was a 54 year old male who had multiple discoid plaques and nodules on the face. The other case was a 83 year old female who showed scaly plaques, nodules, pustules, and photosensitivity on the face. Histologic exarnination revealed fungus spores and mycelia within the inflamed hair follicles. (Kar J Dermatol 3): 414 418, 1901)

백서 피부에서 식이 아연결핍이 산소 free radical 방어체계에 관여하는 수종의 효소활성에 미치는 영향

김동건(Dong Kun Kim),구상완(Sang Wahn Koo),이은소(Eun So Lee),이정복(Jung Bock Lee) 대한피부과학회 1992 대한피부과학회지 Vol.30 No.3

The effect of dietary zinc deficiency on the enzymatic components of free radical defense system was observed in the skin of rats. We measured the concentration of serum zinc and the enzymatic activities of CuZn superoxide dismutase(CuZn SOD), glucose-6-phosphate dehydrogenase(GGPDH) and glutathione reductase (GSH-RD). The serum zinc level was sig nificantly lower in the zinc-deficient group compared to the zinc-supplemented group after 8 weeks of consuming the diet(P<0.01). CuZn SOD activity was not different between the two groups after 4 weeks. The Zn deficient group showed the significantly decreased activity of G6PDH after 4 and 8 weeks of consuming the diet(P<0.01). The activity of GSH-RD was increased in the zinc-deficient group compared to the supplemented group after 4 weeks of consuming the diet(P<0.01), but after 8 weeks the activity was not different between the two groups. From the results obtained, it could be concluded that GSH-RD may contribute to the oxygen free radical defense system in zinc deficiency in the earlier weeks of consum ing the zinc-deficient diet. (Kor J Dermatol 1992; 30(3): 334-339)

사람피부의 해부학적 위치에 따른 피부장벽 손상후 회복율 및 피부표면 지질조성의 차이

이승헌 ( Seung Hun Lee ),구상완 ( Sang Wahn Koo ),황미선 ( Mee Seon Hwang ),이원주 ( Won Joo Lee ),김도원 ( Do Won Kim ) 대한피부과학회 1996 대한피부과학회지 Vol.34 No.1

Background: Lipids in the stratum corneum which acts as a barrier prevents transepidermal water loss and percutaneous absorption of a substance differs at different anatomic sites of skin. No study has yet been done on the recovery rate of the skin barrier after its destruction in relation to anatomic sites. Objectives : The purpose of this study was to measure the recovery rate of the skin barrier after its disruption in relation to anatomic sites and also to examine the lipid composition of skin surface lipids at different anatomic sites of skin. Methods : The study population consisted of 20 healthy male subjects. We used 3M Scotch tapes to remove the stratum corneum and examined the recovery rate by measuring the TEWL using an evaporimeter after 2.5, 6, 10, 24, 48 and 72 hours at eight different anatomic sites consisting of the post-auricular area, chest, abdomen, back, forearm, thigh and calf. The skin surface lipids were extracted using 99% ethanol at ten different anatomic sites including the face, chest, abdomen, back, upper arm, forearm, palm, thigh, shin and sole. The composition of skin surface lipids was determined by thin layer chromatography. Results : 1. The transepidermal water loss was highest in the post-auricular area and ahdomen, back, thigh, forearm, upper arm, thieh and chest in a decreasing order, although there was no statistical significance(p>0.05). 2. The recovery rate was highest in the post-auricular area after 2.5 hours with a statistical significance compared to other sites except for the abdomen(p<0.05). 3. The recovery rate was also highest in the post-auricular area after 6, 10, 24, 48 and 72hrs compared with other sites with a statistical significance (p<0.01). 4. Lipid analysis of ten anatomic sites demonstrated that the concentration of cholesterol sulfate was highest on the face, that of cholesterol was highest on the back and that of sphingolipid was highest on the abdomen but there was no statistical significance. Cpnclusion : The post-auricular area showed the fastest recovery rate after its barrier disruption which can be applied in the percutaneous absorption of substances. Also the composition of human skin surface lipids, especially epidermal lipids at different anatomic sites was not statistically different. (Kor J Dermatol 1996;34(1): 38-45)

비후형 심상성 루푸스

최용섭(Yong Seop Choi),한승경(Seung Kyung Hann),원진형(Jin Hyung Won),구상완(Sang Wahn Koo) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.3

We report a case of hypertrophic lupus vulgaris in the right knee of an 18-year-old man. The lesion, an erythematous scaly hypertrophic plaque, had grown for 12 years without any trestment leaving an atrophic scar. Histopathologically, the lesion consisted of s granuloma composed of epithelioid cells and lymphocytes. Lsboratory examinations including hematology, blood chemistry, urinalysis, and chest roentgenography were normal. The Mantoux test was strongly positive. Treatment with anti-tuberculosis medieation resulted in a good response. (Kor J Dermatol 20(3): 410 422,1001)

서울의 PPNG 발생 빈도(1991-1992)

김재홍,윤기범,박평원,김영진,전경민,김영태,김중환,곽호,구상완,송민석,유옥,지혜구,김동원,문상은,박영립,정승호,성범진,성순제,엄주용,황정열,이기홍,이주협,전태진 대한화학요법학회 1994 대한화학요법학회지 Vol.12 No.1

The prevalence of PPNG among pretreated gonorrhea cases isolated at the STD clinic of Choong-Ku Public Health Center in Seoul has been studied and reported annually since 1981. In 1991, 123 strains of N.gonorrhoeae were isolated, among which 58(47.1%) were PPNG. In 1992, 98 starains of N.gonorrhoeae were isolated, among which 51(52.0%) were PPNG. In all, 109(49.3%) strains were found to be PPNG among 221 strains isolated between 1991-1992. The prevalence of PPNG in Seoul showed increased tendency till 1989, thereafter, it has been stationary or slightly decreasing.

Rowell 증후군 1 예

김영근,구상완,최광성,원덕환,임태균 대한피부과학회 2001 대한피부과학회지 Vol.39 No.7

Rowell's syndrome is believed to be a distinct and rare clinical entity originally described as lupus erythematosus associated with erythema multiforme-like skin lesions, immunologic findings of speckled antinuclear antibodies, anti-La antibodies and a positive test for rheumatoid factor. We report a case of systemic lupus erythematosus in a 31-year-old woman with annular erythematous patches on both hands, feet and back. Also, she presented abnormal serologic and immunologic findings. We believed that our patient meets the criteria for this rarely reported entity.

정맥내 발생한 화농성 육아종 1예

김영근,구상완,이주흥,원덕환,반대현,최광성 대한피부과학회 1999 대한피부과학회지 Vol.37 No.11

An intravenous pyogenic granuloma is a rare, benign, intravascular tumor, which arises from the vein wall and protrudes into the lumen. This is characterized by a lobular proliferation of capillaries similar to the more common cutaneous pyogenic granulomas. We report a case of intravenous pyogenic granuloma which showed lobular capillary proliferation in the perivenous connective tissue.