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Virus Incidence of Sweet Potato in Korea from 2011 to 2014
김재덕,양정욱,곽혜련,김미경,서장균,정미남,이향언,이경보,남상식,김창석,이관석,김정수,이석찬,최홍수 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.5
A nationwide survey was performed to investigate the current incidence of viral diseases in Korean sweet potatoes for germplasm and growing fields from 2011 to 2014. A total of 83.8% of the germplasm in Korea was infected with viruses in 2011. Commercial cultivars that were used to supply growing fields were infected at a rate of 62.1% in 2012. Among surveyed viruses, the incidence of five Potyvirus species that infect sweet potato decreased between 2012 and 2013, and then increased again in 2014. Representatively, the incidence of Sweet potato feathery mottle virus (SPFMV) was 87.0% in 2012, 20.7% in 2013 and then increased to 35.3% in 2014. Unlike RNA viruses, DNA viruses were shown to decrease continuously. The incidence of Sweet potato leaf curl virus (SPLCV) was 5.5% in 2003, 59.5% in 2011, and 47.4% in 2012. It then decreased continuously year by year to 33.2% in 2013, and then 25.6% in 2014. While the infection rate of each virus species showed a tendency to decline, the virus infection status was more variable in 2013 and 2014. Nevertheless, the high rate of single infections and mixed infection combinations were more variable than the survey results from 2012. As shown in the results from 2013, the most prevalent virus infection was a single infection at 27.6%, with the highest rate of infection belonging to sweet potato symptomless virus-1 (SPSMV-1) (12.9%). Compared to 2013, infection combinations were more varied in 2014, with a total of 122 kinds of mixed infection.
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김지영,노현모,최준호,최성일,곽혜련 한국유전학회 1993 Genes & Genomics Vol.15 No.1
A rat liver cDNA library was screened for a prothrombin cDNA using a cDNA fragment coding human prothrombin as a probe. Among about 50,000 plaques, 30 positive plaques were obtained by hybridization with ^(32)P-labelled probe. The cDNA insert from two of the positive λ DNA were selected for further analysis of the prothrombin cDNA. The DNA sequence of the cDNA coding a rat prothrombin has been determined. It consists of 1950 nucleotides and encodes an ORF of 1851 nucleotides, 22 bp noncoding regions at 5' end and 97 bp noncoding regions at 3' end. It does not have poly A tail and poly A addition signal, AATAAA. Our cloned prothrombin cDNA differs by 6 nucleotides from already cloned rat prothrombin cDNA. It shows 80% homology with human prothrombin. Comparative analysis of region having considerable sequence homology between human and rat prothrombin suggests that the functional and structural domains and post translational modification sites in rat prothrombin cDNA can be mapped. The signal peptide, the cleavage sites, γ-carboxylated region, glycosylation attachment sites and the cleavage sites by Factor Xa in rat prothrombin can be presumed by comparative analysis. The rat prothrombin cDNA was expressed in E. coli as a fusion protein using glutathione S-transferase vector system.
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입원노인의 삶의 질, 죽음 불안, 영적 안녕에 관한 연구
김연숙,권영은,성기순,곽혜련 노인간호학회 2007 노인간호학회지 Vol.9 No.2
Purpose: The purpose of this study was to provide appropriate spiritual and comprehensive nursing care for elders admitted to hospital by identifying factors that affect their quality of life, anxiety about death, and spiritual well being and by identifying correlations between these variables. Method: From April 23 to June 30, 2007, 76 patients 65 years of age or older, admitted to the Bobath Memorial Hospital were enrolled in the study. The elders were questioned about their quality of life, anxiety about death, spiritual well being, and their demographic characteristics. The data were analyzed using t-t.est. ANOVA, and coefficient with SPSS for Window version 10.1. Result: This study shows that for most of the elders, quality of life was found to be higher than average at 3.29 out of 5.00. Also, anxiety about death was found to be higher than average at 2.45 out of 4.00. However, spiritual well being was found to be lower at 2.75 out of 6.00. The study showed that as quality of life improves, anxiety about death was reduced. There was no direct relation between quality of life and spiritual well being. There was no direct relation between anxiety about death and spiritual well being. Quality of life for elders was found to be related to gender (p=0.008), economic status (p=0.019), and relationship with family (p=O.OOO). Anxiety death was affected most by job (p= 0.024) and period of hospitalization (p =0.043). Spiritual well being was affected by education(p=0.014), religion (p=O.OOO), and children (p=0.028). Conclusion: The purpose of the study was to improve nursing services for elders admitted to hospital to improve the quality of their lives and spiritual well being and to reduce anxiety about death.
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Occurrence and Multiplex PCR Detection of Citrus Yellow Vein Clearing Virus in Korea
Taemin Jin,Ji-Kwang Kim,Hee-Seong Byun,Hong-Soo Choi,Byeongjin Cha,곽혜련,김미경 한국식물병리학회 2024 Plant Pathology Journal Vol.40 No.1
Citrus yellow vein clearing virus (CYVCV) is a member of the Alphaflexiviridae family that causes yellow vein clearing symptoms on citrus leaves. A total of 118 leaf samples from nine regions of six provinces in Korea were collected from various citrus species in 2020 and 2021. Viral diagnosis using next-generation sequencing and reverse transcription polymerase chain reaction (RT-PCR) identified four viruses: citrus tristeza virus, citrus leaf blotch virus, citrus vein enation virus, and CYVCV. A CYVCV incidence of 9.3% was observed in six host plants, including calamansi, kumquat, Persian lime, and Eureka lemon. Among the citrus infected by CYVCV, only three samples showed a single infection; the other showed a mixed infection with other viruses. Eureka lemon and Persian lime exhibited yellow vein clearing, leaf distortion, and water-soak symptom underside of the leaves, while the other hosts showed only yellowing symptoms on the leaves. The complete genome sequences were obtained from five CYVCV isolates. Comparison of the isolates reported from the different geographical regions and hosts revealed the high sequence identity (95.2% to 98.8%). Phylogenetic analysis indicated that all the five isolates from Korea were clustered into same clade but were not distinctly apart from isolates from China, Pakistan, India, and Türkiye. To develop an efficient diagnosis system for the four viruses, a simultaneous detection method was constructed using multiplex RT-PCR. Sensitivity evaluation, simplex RT-PCR, and stability testing were conducted to verify the multiplex RT-PCR system developed in this study. This information will be useful for developing effective disease management strategies for citrus growers in Korea.
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