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남해안 양식산 조피볼락에 기생한 Microcotyle sebastisci 의 감염률 변동
최상덕,공용근,방인철,심두생,백재민 한국어병학회 1996 한국어병학회지 Vol.9 No.2
1995년 4월부터 10월까지 전남 가막만과 경남 남해 해역에서 양식하는 조피볼락에 기생하는 아가미흡충 Microcotyle sebastisci의 기생률 변동 및 병리조직학적 변화를 조사하였다. M. sebastisci는 6∼7월에 나타나지 않았고, 주로 새엽 II∼III에 기생하였다. 기생된 부위는 미세한 출혈과 함께 점액이 다량 분비되었다. 남해의 경우 9월에 있어서 감염률, 상대 감염밀도, 평균 감염강도는 각각 40.0%, 30.7, 76.8을 나타내었다. 또한 가막만의 경우는 10월에 감염률, 상대 감염밀도, 평균 감염강도는 각각 46.0%, 40.5, 88.0으로 조사기간 중 가장 높게 나타났다. 병리조직학적 소견으로는 호흡상피세포의 증생, 비후, 새박판간상피세포의 중생과 새변의 곤봉화가 일어났다. 2차적으로 세균의 감염에 의한 아가미 부식도 관찰되었다. We report on the variation of infection and histopathological change of Microcotyle sebastisci parasitic on cultured rockfish, Sebastes schlegeli, in Namhae Islands and Kamak Bay from April to October in 1995. Microcotylosis due to Microcotyle sebastisci occurred among cultured rockfish, Sebastes schlegeli. This parasite was not found on the host fish from June to July. Parasite sites were mainly consist of 2nd and 3rd gill arch's filaments of rockfish. Also, the sites were secreted in large quantity of mucus with a very small bleeding. In Namhae Islands, maximum values of prevalence, relative density and mean intensity were found on September 1995, as 40.0%, 30.7 and 76.8, respectively. In Kamak Bay, maximum values of prevalence, relative density and mean intensity were obtained on October 1995, as 46.0%, 40.5 and 88.0, respectively. Histopathological changes of the heavily infested gills were showed necrosis, epithelium of the gill filaments underwent hyperplasia with fusion of the lamella and filamental clubbing. And a bacterial colony is invaded on the surface of lamella epithelium.
해산 녹조료 참홑파래 , Monostroma nitidum 의 원형질체 분리와 분화
조용철(Yong Chul Cho),공용근(Yong Gun Gong),윤장택(Jang Taek Yoon),선상미(Sang Mi Sun),정규화(Gyu Hwa Chung) 한국수산과학회 1999 한국수산과학회지 Vol.32 No.1
High yields of protoplasts were obtained following enzymatic digestion of the vegetative thalli of marine green alga Monostroma nitidum. The enzyme mixtures containing 4% Cellulase R-10+3% Macerozyme R-10+3% Abalone acetone powder produced 4.41×10^6 protoplasts per 300 ㎎ of fresh tissue. The highest yield of protoplasts was obtained by 270 minutes treatment of the thalli in enzyme solution. Freshly isolated protoplasts were spherical in shape and ranged between 13∼33 ㎛ in diameter. The high efficiency of differentiation were obtained by incubating freshly isolated protoplasts in 0.4 M mannitol f/2 medium for 7 days and then transferring to 0.2 M mannitol f/2 medium. Protoplasts began to form new cell walls three days after initial culture and began to germinate after 10 days, and then form a leafy thallus after further culture in f/2 medium. The addition of antibiotics in media inhibited the differentiation of protoplasts in culture.